The influence of the route of inoculation on the development of the leptospiral infection in hamsters (Mesocricetus auratus) experimentally infected with Leptospira interrogans serovar pomona
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/6277 |
Resumo: | The influence of the route of inoculation on the development of the leptospiral infection was investigated in 60 hamsters (Mesocricetus auratos) experimentally infected with Leptospira interrogans serovar pomona. The routes of inoculation were intraperitoneal, subcutaneous, oral, conjuntival and scrapped skin. All animals received the leptospiral inoculum cultivated in Fletcher's medium, with 20 to 30 leptospires per field under 200x magnification. The controls were inoculated only with Fletcher's medium. Samples of blood sera and the kidney fragments were collected in the agonic stage of the disease or at 21st experimental day, when all survivors were killed. The antibodies detection was done by the microscopic agglutination test and the bacteria detection by the dark field microscopic examination and culture in Fletcher's medium, by the tenfold dilutions technique. The presence and development of disease was affected by the route of infection. The oral route was the less effective for promoting the disease. There was no association between the frequency of carrier state and the route of infection. |
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USP-49 |
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Brazilian Journal of Veterinary Research and Animal Science |
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The influence of the route of inoculation on the development of the leptospiral infection in hamsters (Mesocricetus auratus) experimentally infected with Leptospira interrogans serovar pomonaInfluência da via de inoculação sobre o estabelecimento e a evolução da leptospirose em hamsters (Mesocricetus auratus) experimentalmente infectados com Leptospira interrogans sorovar pomonaVia de inoculaçãoHamstersSorovar pomonaRout of inoculationHamstersSerovar pomonaThe influence of the route of inoculation on the development of the leptospiral infection was investigated in 60 hamsters (Mesocricetus auratos) experimentally infected with Leptospira interrogans serovar pomona. The routes of inoculation were intraperitoneal, subcutaneous, oral, conjuntival and scrapped skin. All animals received the leptospiral inoculum cultivated in Fletcher's medium, with 20 to 30 leptospires per field under 200x magnification. The controls were inoculated only with Fletcher's medium. Samples of blood sera and the kidney fragments were collected in the agonic stage of the disease or at 21st experimental day, when all survivors were killed. The antibodies detection was done by the microscopic agglutination test and the bacteria detection by the dark field microscopic examination and culture in Fletcher's medium, by the tenfold dilutions technique. The presence and development of disease was affected by the route of infection. The oral route was the less effective for promoting the disease. There was no association between the frequency of carrier state and the route of infection.Foi investigada a influência da via de inoculação sobre o estabelecimento e a evolução da leptospirose em hamsters (Mesocricetus auratus) experimentalmente infectados com Leptospira interrogans sorovar pomona. As vias de inoculação ensaiadas foram: intraperitoneal, subcutânea, oral, conjuntival e escarificação cutânea. O inóculo infeccioso foi constituído por uma cultura em meio de Fletcher, com 20 a 30 leptospiras por campo microscópio no aumento de 200 vezes. Os animais controle foram inoculados apenas com meio de Fletcher. Foram colhidas amostras de soro sanguíneo e fragmentos de rins na fase agônica da doença ou no 21º dia pós-infecção, quando todos os animais foram sacrificados. Para a pesquisa de leptospiras, foi feito o exame direto com microscopia óptica em campo escuro e cultivo em meio de Fletcher, pela técnica das diluições seriadas. A detecção de aglutininas anti-leptospiras foi realizada pela técnica de soroaglutinação microscópica. A instalação e evolução da leptospirose foram afetadas pela via de inoculação. A via oral foi a menos efetiva em estabelecer a infecção. Não foi observada associação estatística entre a freqüência de portadores e a via de inoculação.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2004-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/627710.1590/S1413-95962004000300008Brazilian Journal of Veterinary Research and Animal Science; Vol. 41 Núm. 3 (2004); 194-200 Brazilian Journal of Veterinary Research and Animal Science; Vol. 41 No. 3 (2004); 194-200 Brazilian Journal of Veterinary Research and Animal Science; v. 41 n. 3 (2004); 194-200 Brazilian Journal of Veterinary Research and Animal Science; V. 41 N. 3 (2004); 194-200 1678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/6277/7808Macedo, Nicodemos Alves deMorais, Zenáide Maria deCamargo, Cláudio Roberto de AlmeidaAlves, Clebert JoséAzevedo, Sérgio Santos deNümberger Júnior, RodolfoVasconcellos, Sílvio Arrudainfo:eu-repo/semantics/openAccess2020-06-23T04:29:33Zoai:revistas.usp.br:article/6277Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:35.117229Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
The influence of the route of inoculation on the development of the leptospiral infection in hamsters (Mesocricetus auratus) experimentally infected with Leptospira interrogans serovar pomona Influência da via de inoculação sobre o estabelecimento e a evolução da leptospirose em hamsters (Mesocricetus auratus) experimentalmente infectados com Leptospira interrogans sorovar pomona |
title |
The influence of the route of inoculation on the development of the leptospiral infection in hamsters (Mesocricetus auratus) experimentally infected with Leptospira interrogans serovar pomona |
spellingShingle |
The influence of the route of inoculation on the development of the leptospiral infection in hamsters (Mesocricetus auratus) experimentally infected with Leptospira interrogans serovar pomona Macedo, Nicodemos Alves de Via de inoculação Hamsters Sorovar pomona Rout of inoculation Hamsters Serovar pomona |
title_short |
The influence of the route of inoculation on the development of the leptospiral infection in hamsters (Mesocricetus auratus) experimentally infected with Leptospira interrogans serovar pomona |
title_full |
The influence of the route of inoculation on the development of the leptospiral infection in hamsters (Mesocricetus auratus) experimentally infected with Leptospira interrogans serovar pomona |
title_fullStr |
The influence of the route of inoculation on the development of the leptospiral infection in hamsters (Mesocricetus auratus) experimentally infected with Leptospira interrogans serovar pomona |
title_full_unstemmed |
The influence of the route of inoculation on the development of the leptospiral infection in hamsters (Mesocricetus auratus) experimentally infected with Leptospira interrogans serovar pomona |
title_sort |
The influence of the route of inoculation on the development of the leptospiral infection in hamsters (Mesocricetus auratus) experimentally infected with Leptospira interrogans serovar pomona |
author |
Macedo, Nicodemos Alves de |
author_facet |
Macedo, Nicodemos Alves de Morais, Zenáide Maria de Camargo, Cláudio Roberto de Almeida Alves, Clebert José Azevedo, Sérgio Santos de Nümberger Júnior, Rodolfo Vasconcellos, Sílvio Arruda |
author_role |
author |
author2 |
Morais, Zenáide Maria de Camargo, Cláudio Roberto de Almeida Alves, Clebert José Azevedo, Sérgio Santos de Nümberger Júnior, Rodolfo Vasconcellos, Sílvio Arruda |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Macedo, Nicodemos Alves de Morais, Zenáide Maria de Camargo, Cláudio Roberto de Almeida Alves, Clebert José Azevedo, Sérgio Santos de Nümberger Júnior, Rodolfo Vasconcellos, Sílvio Arruda |
dc.subject.por.fl_str_mv |
Via de inoculação Hamsters Sorovar pomona Rout of inoculation Hamsters Serovar pomona |
topic |
Via de inoculação Hamsters Sorovar pomona Rout of inoculation Hamsters Serovar pomona |
description |
The influence of the route of inoculation on the development of the leptospiral infection was investigated in 60 hamsters (Mesocricetus auratos) experimentally infected with Leptospira interrogans serovar pomona. The routes of inoculation were intraperitoneal, subcutaneous, oral, conjuntival and scrapped skin. All animals received the leptospiral inoculum cultivated in Fletcher's medium, with 20 to 30 leptospires per field under 200x magnification. The controls were inoculated only with Fletcher's medium. Samples of blood sera and the kidney fragments were collected in the agonic stage of the disease or at 21st experimental day, when all survivors were killed. The antibodies detection was done by the microscopic agglutination test and the bacteria detection by the dark field microscopic examination and culture in Fletcher's medium, by the tenfold dilutions technique. The presence and development of disease was affected by the route of infection. The oral route was the less effective for promoting the disease. There was no association between the frequency of carrier state and the route of infection. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/6277 10.1590/S1413-95962004000300008 |
url |
https://www.revistas.usp.br/bjvras/article/view/6277 |
identifier_str_mv |
10.1590/S1413-95962004000300008 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/6277/7808 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 41 Núm. 3 (2004); 194-200 Brazilian Journal of Veterinary Research and Animal Science; Vol. 41 No. 3 (2004); 194-200 Brazilian Journal of Veterinary Research and Animal Science; v. 41 n. 3 (2004); 194-200 Brazilian Journal of Veterinary Research and Animal Science; V. 41 N. 3 (2004); 194-200 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051556343840768 |