The influence of the route of inoculation on the development of the leptospiral infection in hamsters (Mesocricetus auratus) experimentally infected with Leptospira interrogans serovar pomona

Detalhes bibliográficos
Autor(a) principal: Macedo, Nicodemos Alves de
Data de Publicação: 2004
Outros Autores: Morais, Zenáide Maria de, Camargo, Cláudio Roberto de Almeida, Alves, Clebert José, Azevedo, Sérgio Santos de, Nümberger Júnior, Rodolfo, Vasconcellos, Sílvio Arruda
Tipo de documento: Artigo
Idioma: por
Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
Texto Completo: https://www.revistas.usp.br/bjvras/article/view/6277
Resumo: The influence of the route of inoculation on the development of the leptospiral infection was investigated in 60 hamsters (Mesocricetus auratos) experimentally infected with Leptospira interrogans serovar pomona. The routes of inoculation were intraperitoneal, subcutaneous, oral, conjuntival and scrapped skin. All animals received the leptospiral inoculum cultivated in Fletcher's medium, with 20 to 30 leptospires per field under 200x magnification. The controls were inoculated only with Fletcher's medium. Samples of blood sera and the kidney fragments were collected in the agonic stage of the disease or at 21st experimental day, when all survivors were killed. The antibodies detection was done by the microscopic agglutination test and the bacteria detection by the dark field microscopic examination and culture in Fletcher's medium, by the tenfold dilutions technique. The presence and development of disease was affected by the route of infection. The oral route was the less effective for promoting the disease. There was no association between the frequency of carrier state and the route of infection.
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spelling The influence of the route of inoculation on the development of the leptospiral infection in hamsters (Mesocricetus auratus) experimentally infected with Leptospira interrogans serovar pomonaInfluência da via de inoculação sobre o estabelecimento e a evolução da leptospirose em hamsters (Mesocricetus auratus) experimentalmente infectados com Leptospira interrogans sorovar pomonaVia de inoculaçãoHamstersSorovar pomonaRout of inoculationHamstersSerovar pomonaThe influence of the route of inoculation on the development of the leptospiral infection was investigated in 60 hamsters (Mesocricetus auratos) experimentally infected with Leptospira interrogans serovar pomona. The routes of inoculation were intraperitoneal, subcutaneous, oral, conjuntival and scrapped skin. All animals received the leptospiral inoculum cultivated in Fletcher's medium, with 20 to 30 leptospires per field under 200x magnification. The controls were inoculated only with Fletcher's medium. Samples of blood sera and the kidney fragments were collected in the agonic stage of the disease or at 21st experimental day, when all survivors were killed. The antibodies detection was done by the microscopic agglutination test and the bacteria detection by the dark field microscopic examination and culture in Fletcher's medium, by the tenfold dilutions technique. The presence and development of disease was affected by the route of infection. The oral route was the less effective for promoting the disease. There was no association between the frequency of carrier state and the route of infection.Foi investigada a influência da via de inoculação sobre o estabelecimento e a evolução da leptospirose em hamsters (Mesocricetus auratus) experimentalmente infectados com Leptospira interrogans sorovar pomona. As vias de inoculação ensaiadas foram: intraperitoneal, subcutânea, oral, conjuntival e escarificação cutânea. O inóculo infeccioso foi constituído por uma cultura em meio de Fletcher, com 20 a 30 leptospiras por campo microscópio no aumento de 200 vezes. Os animais controle foram inoculados apenas com meio de Fletcher. Foram colhidas amostras de soro sanguíneo e fragmentos de rins na fase agônica da doença ou no 21º dia pós-infecção, quando todos os animais foram sacrificados. Para a pesquisa de leptospiras, foi feito o exame direto com microscopia óptica em campo escuro e cultivo em meio de Fletcher, pela técnica das diluições seriadas. A detecção de aglutininas anti-leptospiras foi realizada pela técnica de soroaglutinação microscópica. A instalação e evolução da leptospirose foram afetadas pela via de inoculação. A via oral foi a menos efetiva em estabelecer a infecção. Não foi observada associação estatística entre a freqüência de portadores e a via de inoculação.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2004-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/627710.1590/S1413-95962004000300008Brazilian Journal of Veterinary Research and Animal Science; Vol. 41 Núm. 3 (2004); 194-200 Brazilian Journal of Veterinary Research and Animal Science; Vol. 41 No. 3 (2004); 194-200 Brazilian Journal of Veterinary Research and Animal Science; v. 41 n. 3 (2004); 194-200 Brazilian Journal of Veterinary Research and Animal Science; V. 41 N. 3 (2004); 194-200 1678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/6277/7808Macedo, Nicodemos Alves deMorais, Zenáide Maria deCamargo, Cláudio Roberto de AlmeidaAlves, Clebert JoséAzevedo, Sérgio Santos deNümberger Júnior, RodolfoVasconcellos, Sílvio Arrudainfo:eu-repo/semantics/openAccess2020-06-23T04:29:33Zoai:revistas.usp.br:article/6277Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:35.117229Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv The influence of the route of inoculation on the development of the leptospiral infection in hamsters (Mesocricetus auratus) experimentally infected with Leptospira interrogans serovar pomona
Influência da via de inoculação sobre o estabelecimento e a evolução da leptospirose em hamsters (Mesocricetus auratus) experimentalmente infectados com Leptospira interrogans sorovar pomona
title The influence of the route of inoculation on the development of the leptospiral infection in hamsters (Mesocricetus auratus) experimentally infected with Leptospira interrogans serovar pomona
spellingShingle The influence of the route of inoculation on the development of the leptospiral infection in hamsters (Mesocricetus auratus) experimentally infected with Leptospira interrogans serovar pomona
Macedo, Nicodemos Alves de
Via de inoculação
Hamsters
Sorovar pomona
Rout of inoculation
Hamsters
Serovar pomona
title_short The influence of the route of inoculation on the development of the leptospiral infection in hamsters (Mesocricetus auratus) experimentally infected with Leptospira interrogans serovar pomona
title_full The influence of the route of inoculation on the development of the leptospiral infection in hamsters (Mesocricetus auratus) experimentally infected with Leptospira interrogans serovar pomona
title_fullStr The influence of the route of inoculation on the development of the leptospiral infection in hamsters (Mesocricetus auratus) experimentally infected with Leptospira interrogans serovar pomona
title_full_unstemmed The influence of the route of inoculation on the development of the leptospiral infection in hamsters (Mesocricetus auratus) experimentally infected with Leptospira interrogans serovar pomona
title_sort The influence of the route of inoculation on the development of the leptospiral infection in hamsters (Mesocricetus auratus) experimentally infected with Leptospira interrogans serovar pomona
author Macedo, Nicodemos Alves de
author_facet Macedo, Nicodemos Alves de
Morais, Zenáide Maria de
Camargo, Cláudio Roberto de Almeida
Alves, Clebert José
Azevedo, Sérgio Santos de
Nümberger Júnior, Rodolfo
Vasconcellos, Sílvio Arruda
author_role author
author2 Morais, Zenáide Maria de
Camargo, Cláudio Roberto de Almeida
Alves, Clebert José
Azevedo, Sérgio Santos de
Nümberger Júnior, Rodolfo
Vasconcellos, Sílvio Arruda
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Macedo, Nicodemos Alves de
Morais, Zenáide Maria de
Camargo, Cláudio Roberto de Almeida
Alves, Clebert José
Azevedo, Sérgio Santos de
Nümberger Júnior, Rodolfo
Vasconcellos, Sílvio Arruda
dc.subject.por.fl_str_mv Via de inoculação
Hamsters
Sorovar pomona
Rout of inoculation
Hamsters
Serovar pomona
topic Via de inoculação
Hamsters
Sorovar pomona
Rout of inoculation
Hamsters
Serovar pomona
description The influence of the route of inoculation on the development of the leptospiral infection was investigated in 60 hamsters (Mesocricetus auratos) experimentally infected with Leptospira interrogans serovar pomona. The routes of inoculation were intraperitoneal, subcutaneous, oral, conjuntival and scrapped skin. All animals received the leptospiral inoculum cultivated in Fletcher's medium, with 20 to 30 leptospires per field under 200x magnification. The controls were inoculated only with Fletcher's medium. Samples of blood sera and the kidney fragments were collected in the agonic stage of the disease or at 21st experimental day, when all survivors were killed. The antibodies detection was done by the microscopic agglutination test and the bacteria detection by the dark field microscopic examination and culture in Fletcher's medium, by the tenfold dilutions technique. The presence and development of disease was affected by the route of infection. The oral route was the less effective for promoting the disease. There was no association between the frequency of carrier state and the route of infection.
publishDate 2004
dc.date.none.fl_str_mv 2004-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/6277
10.1590/S1413-95962004000300008
url https://www.revistas.usp.br/bjvras/article/view/6277
identifier_str_mv 10.1590/S1413-95962004000300008
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/6277/7808
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 41 Núm. 3 (2004); 194-200
Brazilian Journal of Veterinary Research and Animal Science; Vol. 41 No. 3 (2004); 194-200
Brazilian Journal of Veterinary Research and Animal Science; v. 41 n. 3 (2004); 194-200
Brazilian Journal of Veterinary Research and Animal Science; V. 41 N. 3 (2004); 194-200
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
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