Efficiency and effect of consecutive embryo recoveries on the reproductive system of goat donnors
Autor(a) principal: | |
---|---|
Data de Publicação: | 1999 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/5757 |
Resumo: | The purpose of this trial was to compare the efficiency and effect of consecutive embryo recoveries by three different methods (T1 - transcervical; T2 laparoscopy and T3 laparotomy) on the reproductive activity of goat donors. Ten goats were allocated into each treatment (T1, T2 and T3) and submitted to three consecutive embryo recoveries. These were performed 56 days apart. The superovulation begun on 8th day of oestrus synchronization and all goats received 250 UI of porcine FSH splited into eight decreasing dosages at 12 hours intervals. The embryo recovery took place on the 5th or 6th day from the last mating. Fifty-six days after the third recovery of embryos, the animals were sacrified and the genital tract was evaluated. The time spent to recovery the embryos was 21min 32sec; 37min 14sec and 56min 22sec, respectively to T1; T2 and T3 (p<0.01). T3 showed the highest recovery rate of washing solution (83.7%), followed by T2 (72.2%) and T1 (64.3%) (p<0.05). Embryo recovery rate had as mean values, 57.1; 81.1 and 27.3%, respectivily to T1, T2 and T3. The variation ranged between 0 and 100%. Several factors affected the embryo recovery rate, such as the presence of demised corpora lutea, the ovulation rate and the presence of adhesions in the genital tract. Nonetheless, embryo recevery rate was acceptable in all treatments. The T1 caused eversion of endometrium and adherence between the uterine horn and epiploon in one animal, the T2 caused 30, 40 and 60% of endometrium eversion and 10, 10 and 70% adherence on genital tract, respectively, for the first, second and third embryo recovery. The T3 caused 80% of adherence on genital tract after the first embryo recovery and 100% after the second. It is possible to use the methods T1 and T2 to recovery embryos in goats. |
id |
USP-49_53f3b62c1a4a92a9560eb1f388e33b5f |
---|---|
oai_identifier_str |
oai:revistas.usp.br:article/5757 |
network_acronym_str |
USP-49 |
network_name_str |
Brazilian Journal of Veterinary Research and Animal Science |
repository_id_str |
https://www.revistas.usp.br/bjvras/index |
spelling |
Efficiency and effect of consecutive embryo recoveries on the reproductive system of goat donnorsEficiência da recuperação de embriões e os efeitos de consecutivas colheitas sobre o aparelho reprodutor de doadoras da espécie caprinaCaprinosEmbriõesReproduçãoGoatEmbryoReproductionThe purpose of this trial was to compare the efficiency and effect of consecutive embryo recoveries by three different methods (T1 - transcervical; T2 laparoscopy and T3 laparotomy) on the reproductive activity of goat donors. Ten goats were allocated into each treatment (T1, T2 and T3) and submitted to three consecutive embryo recoveries. These were performed 56 days apart. The superovulation begun on 8th day of oestrus synchronization and all goats received 250 UI of porcine FSH splited into eight decreasing dosages at 12 hours intervals. The embryo recovery took place on the 5th or 6th day from the last mating. Fifty-six days after the third recovery of embryos, the animals were sacrified and the genital tract was evaluated. The time spent to recovery the embryos was 21min 32sec; 37min 14sec and 56min 22sec, respectively to T1; T2 and T3 (p<0.01). T3 showed the highest recovery rate of washing solution (83.7%), followed by T2 (72.2%) and T1 (64.3%) (p<0.05). Embryo recovery rate had as mean values, 57.1; 81.1 and 27.3%, respectivily to T1, T2 and T3. The variation ranged between 0 and 100%. Several factors affected the embryo recovery rate, such as the presence of demised corpora lutea, the ovulation rate and the presence of adhesions in the genital tract. Nonetheless, embryo recevery rate was acceptable in all treatments. The T1 caused eversion of endometrium and adherence between the uterine horn and epiploon in one animal, the T2 caused 30, 40 and 60% of endometrium eversion and 10, 10 and 70% adherence on genital tract, respectively, for the first, second and third embryo recovery. The T3 caused 80% of adherence on genital tract after the first embryo recovery and 100% after the second. It is possible to use the methods T1 and T2 to recovery embryos in goats.O objetivo deste experimento foi comparar a eficiência e o efeito de consecutivas colheitas de embriões, por três diferentes métodos (transcervical-T1, laparoscopia-T2 e laparotomia-T3), sobre a atividade reprodutiva de doadoras da espécie caprina. Utilizaram-se 10 cabras em cada método (T1, T2 e T3), sendo as colheitas de embriões repetidas três vezes consecutivas, nas mesmas fêmeas, com intervalo de 56 dias. As fêmeas foram sincronizadas com esponjas vaginais impregnadas com 60 mg de acetato de medroxiprogesterona durante 10 dias e 100 µg de cloprostenol aplicados pela via IM no oitavo dia da sincronização. No 8º dia, iniciou-se a superovulação com 250 UI de FSH de origem suína, divididas em oito doses decrescentes, aplicadas em intervalo de 12 horas. As fêmeas foram acasaladas e as colheitas de embriões realizadas no 5º ou 6º dia após a última cobertura. Após 56 dias da terceira colheita de embriões, foram realizados o abate e a necrópsia das doadoras. O tempo necessário para a colheita de embriões em cada método foi de 21 minutos e 32 segundos; 37 minutos e 14 segundos e 56 minutos e 22 segundos, respectivamente, para T1, T2 e T3 (p<0,01). A maior taxa de recuperação da solução de lavagem foi no T3 (83,7%), seguido por T2 (72,2%) e T1 (64,3%) (p<0,05). As taxas médias de recuperação dos embriões foram 57,1; 81,1 e 27,3% para T1, T2 e T3, respectivamente, com variação entre 0-100%. A taxa de recuperação de embriões sofreu influência de vários fatores, como a presença de corpos lúteos regredidos, a taxa de ovulação e a presença de aderências no sistema genital, mas, isoladamente, a taxa de recuperação de embriões foi satisfatória nos três métodos. O T1 causou eversão do endométrio e aderência entre o corno uterino e o epíploo em uma única fêmea, o T2 causou eversão do endométrio em 30%, 40% e 60% e aderências do sistema genital em 10%, 10% e 70% das fêmeas à 1ª, 2ª e 3ª colheitas, respectivamente. O T3 causou aderências no sistema genital em 80% das doadoras após a primeira e 100% após a segunda colheita. O T1 e o T2 permitem o uso de doadoras em repetidas colheitas de embriões, o que não ocorre com o T3, que causa aderências no sistema genital e órgãos circunvizinhos em 100% dos casos.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia1999-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/575710.1590/S1413-95961999000300006Brazilian Journal of Veterinary Research and Animal Science; Vol. 36 Núm. 3 (1999); 136-143Brazilian Journal of Veterinary Research and Animal Science; Vol. 36 No. 3 (1999); 136-143Brazilian Journal of Veterinary Research and Animal Science; v. 36 n. 3 (1999); 136-143Brazilian Journal of Veterinary Research and Animal Science; V. 36 N. 3 (1999); 136-1431678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/5757/64780Andrioli, AliceSimplicio, Aurino AlvesSoares, Adriana TrindadeVisintin, José Antonioinfo:eu-repo/semantics/openAccess2020-06-23T04:43:38Zoai:revistas.usp.br:article/5757Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:20.887388Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Efficiency and effect of consecutive embryo recoveries on the reproductive system of goat donnors Eficiência da recuperação de embriões e os efeitos de consecutivas colheitas sobre o aparelho reprodutor de doadoras da espécie caprina |
title |
Efficiency and effect of consecutive embryo recoveries on the reproductive system of goat donnors |
spellingShingle |
Efficiency and effect of consecutive embryo recoveries on the reproductive system of goat donnors Andrioli, Alice Caprinos Embriões Reprodução Goat Embryo Reproduction |
title_short |
Efficiency and effect of consecutive embryo recoveries on the reproductive system of goat donnors |
title_full |
Efficiency and effect of consecutive embryo recoveries on the reproductive system of goat donnors |
title_fullStr |
Efficiency and effect of consecutive embryo recoveries on the reproductive system of goat donnors |
title_full_unstemmed |
Efficiency and effect of consecutive embryo recoveries on the reproductive system of goat donnors |
title_sort |
Efficiency and effect of consecutive embryo recoveries on the reproductive system of goat donnors |
author |
Andrioli, Alice |
author_facet |
Andrioli, Alice Simplicio, Aurino Alves Soares, Adriana Trindade Visintin, José Antonio |
author_role |
author |
author2 |
Simplicio, Aurino Alves Soares, Adriana Trindade Visintin, José Antonio |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Andrioli, Alice Simplicio, Aurino Alves Soares, Adriana Trindade Visintin, José Antonio |
dc.subject.por.fl_str_mv |
Caprinos Embriões Reprodução Goat Embryo Reproduction |
topic |
Caprinos Embriões Reprodução Goat Embryo Reproduction |
description |
The purpose of this trial was to compare the efficiency and effect of consecutive embryo recoveries by three different methods (T1 - transcervical; T2 laparoscopy and T3 laparotomy) on the reproductive activity of goat donors. Ten goats were allocated into each treatment (T1, T2 and T3) and submitted to three consecutive embryo recoveries. These were performed 56 days apart. The superovulation begun on 8th day of oestrus synchronization and all goats received 250 UI of porcine FSH splited into eight decreasing dosages at 12 hours intervals. The embryo recovery took place on the 5th or 6th day from the last mating. Fifty-six days after the third recovery of embryos, the animals were sacrified and the genital tract was evaluated. The time spent to recovery the embryos was 21min 32sec; 37min 14sec and 56min 22sec, respectively to T1; T2 and T3 (p<0.01). T3 showed the highest recovery rate of washing solution (83.7%), followed by T2 (72.2%) and T1 (64.3%) (p<0.05). Embryo recovery rate had as mean values, 57.1; 81.1 and 27.3%, respectivily to T1, T2 and T3. The variation ranged between 0 and 100%. Several factors affected the embryo recovery rate, such as the presence of demised corpora lutea, the ovulation rate and the presence of adhesions in the genital tract. Nonetheless, embryo recevery rate was acceptable in all treatments. The T1 caused eversion of endometrium and adherence between the uterine horn and epiploon in one animal, the T2 caused 30, 40 and 60% of endometrium eversion and 10, 10 and 70% adherence on genital tract, respectively, for the first, second and third embryo recovery. The T3 caused 80% of adherence on genital tract after the first embryo recovery and 100% after the second. It is possible to use the methods T1 and T2 to recovery embryos in goats. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/5757 10.1590/S1413-95961999000300006 |
url |
https://www.revistas.usp.br/bjvras/article/view/5757 |
identifier_str_mv |
10.1590/S1413-95961999000300006 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/5757/64780 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 36 Núm. 3 (1999); 136-143 Brazilian Journal of Veterinary Research and Animal Science; Vol. 36 No. 3 (1999); 136-143 Brazilian Journal of Veterinary Research and Animal Science; v. 36 n. 3 (1999); 136-143 Brazilian Journal of Veterinary Research and Animal Science; V. 36 N. 3 (1999); 136-143 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051554711207936 |