Diagnostic efficiency of Brucella soluble antigens in immunodiffusion tests and ability to differentiate Brucella abortus strain 19 vaccinated cattle
Autor(a) principal: | |
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Data de Publicação: | 1999 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/5753 |
Resumo: | Three soluble antigens were compared by radial immunodiffusion (RID) and agar gel immunodiffusion (AGID) tests: a native haptene (NH) from Brucella melitensis 16M, and a polysaccharide (PS) from B. abortus 1119-3, both obtained by non-hydrolytic methods, and the (O-Chain) polysaccharide extracted also from B. abortus 1119-3 but using an hydrolytic method. Three groups of bovine sera were tested: a) Naturally infected (n = 76); b) Non-infected (n = 130) and c) S-19 vaccinated (n = 61); the sensitivity (Se), the specificity (Sp) and the ability to differentiate vaccinated (ADV) were determined in each group a, b and c respectively. The highest Se in the RID test (84.3%) was achieved by NH; while the three antigens gave 100% Sp. The O-Chain showed 100% ADV in this test. In the AGID test PS antigen showed the best Se (86.6%), and all antigens showed 100% of Sp and ADV. Finally, for its production qualities and efficiency the antigens PS and NH represent a promising alternative for complementary diagnosis of brucellosis. |
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Brazilian Journal of Veterinary Research and Animal Science |
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Diagnostic efficiency of Brucella soluble antigens in immunodiffusion tests and ability to differentiate Brucella abortus strain 19 vaccinated cattleEficiência diagnóstica de antígenos solúveis de Brucella em testes de imunodifusão e capacidade para diferenciar bovinos vacinados com Brucella abortus CEPA 19BruceloseTestes de imunodifusãoVacinaçãoBovinosrucellosisImmunodiffusion testsVaccinationCattleThree soluble antigens were compared by radial immunodiffusion (RID) and agar gel immunodiffusion (AGID) tests: a native haptene (NH) from Brucella melitensis 16M, and a polysaccharide (PS) from B. abortus 1119-3, both obtained by non-hydrolytic methods, and the (O-Chain) polysaccharide extracted also from B. abortus 1119-3 but using an hydrolytic method. Three groups of bovine sera were tested: a) Naturally infected (n = 76); b) Non-infected (n = 130) and c) S-19 vaccinated (n = 61); the sensitivity (Se), the specificity (Sp) and the ability to differentiate vaccinated (ADV) were determined in each group a, b and c respectively. The highest Se in the RID test (84.3%) was achieved by NH; while the three antigens gave 100% Sp. The O-Chain showed 100% ADV in this test. In the AGID test PS antigen showed the best Se (86.6%), and all antigens showed 100% of Sp and ADV. Finally, for its production qualities and efficiency the antigens PS and NH represent a promising alternative for complementary diagnosis of brucellosis.Foram comparados três antígenos solúveis: um hapteno nativo (NH) de B. melitensis 16M, um polissacarídeo (PS) obtido de B. abortus 1119-3 e outro polissacarídeo de cadeia O (O-Chain) originado também da última Brucella. Os testes de imunodifusão radial (RID) e imunodifusão em gel de ágar (AGID) foram confrontados com as três classes de soros bovinos: a) infectados naturalmente (n = 76), b) não infectados (n = 130) e c) vacinados com B19 (n = 61) reagindo a testes sorológicos clássicos. Foram determinadas a sensibilidade (Se), a especificidade (Sp) e a capacidade para discriminar vacinados (ADV). A Se mais alta (84,3%) no teste RID foi demonstrada pelo antígeno NH, enquanto os três antígenos tiveram 100% de Sp. O antígeno O-Chain teve 100% de ADV nesse teste. O teste AGID com estes antígenos demonstrou 100% Sp e ADV, enquanto o antígeno PS mostrou uma melhor Se (86,6%). Finalmente, por sua qualidade de produção e eficiência, os antígenos PS e NH representam uma alternativa segura e econômica para o diagnóstico suplementar da brucelose.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia1999-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/575310.1590/S1413-95961999000300002Brazilian Journal of Veterinary Research and Animal Science; Vol. 36 Núm. 3 (1999); 116-120Brazilian Journal of Veterinary Research and Animal Science; Vol. 36 No. 3 (1999); 116-120Brazilian Journal of Veterinary Research and Animal Science; v. 36 n. 3 (1999); 116-120Brazilian Journal of Veterinary Research and Animal Science; V. 36 N. 3 (1999); 116-1201678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/5753/64776Daffner, JoséAbalos, PedroPinochet, LautaroScortti, MarielaUrcelay, Santiagoinfo:eu-repo/semantics/openAccess2020-06-23T04:43:46Zoai:revistas.usp.br:article/5753Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:20.671808Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Diagnostic efficiency of Brucella soluble antigens in immunodiffusion tests and ability to differentiate Brucella abortus strain 19 vaccinated cattle Eficiência diagnóstica de antígenos solúveis de Brucella em testes de imunodifusão e capacidade para diferenciar bovinos vacinados com Brucella abortus CEPA 19 |
title |
Diagnostic efficiency of Brucella soluble antigens in immunodiffusion tests and ability to differentiate Brucella abortus strain 19 vaccinated cattle |
spellingShingle |
Diagnostic efficiency of Brucella soluble antigens in immunodiffusion tests and ability to differentiate Brucella abortus strain 19 vaccinated cattle Daffner, José Brucelose Testes de imunodifusão Vacinação Bovinos rucellosis Immunodiffusion tests Vaccination Cattle |
title_short |
Diagnostic efficiency of Brucella soluble antigens in immunodiffusion tests and ability to differentiate Brucella abortus strain 19 vaccinated cattle |
title_full |
Diagnostic efficiency of Brucella soluble antigens in immunodiffusion tests and ability to differentiate Brucella abortus strain 19 vaccinated cattle |
title_fullStr |
Diagnostic efficiency of Brucella soluble antigens in immunodiffusion tests and ability to differentiate Brucella abortus strain 19 vaccinated cattle |
title_full_unstemmed |
Diagnostic efficiency of Brucella soluble antigens in immunodiffusion tests and ability to differentiate Brucella abortus strain 19 vaccinated cattle |
title_sort |
Diagnostic efficiency of Brucella soluble antigens in immunodiffusion tests and ability to differentiate Brucella abortus strain 19 vaccinated cattle |
author |
Daffner, José |
author_facet |
Daffner, José Abalos, Pedro Pinochet, Lautaro Scortti, Mariela Urcelay, Santiago |
author_role |
author |
author2 |
Abalos, Pedro Pinochet, Lautaro Scortti, Mariela Urcelay, Santiago |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Daffner, José Abalos, Pedro Pinochet, Lautaro Scortti, Mariela Urcelay, Santiago |
dc.subject.por.fl_str_mv |
Brucelose Testes de imunodifusão Vacinação Bovinos rucellosis Immunodiffusion tests Vaccination Cattle |
topic |
Brucelose Testes de imunodifusão Vacinação Bovinos rucellosis Immunodiffusion tests Vaccination Cattle |
description |
Three soluble antigens were compared by radial immunodiffusion (RID) and agar gel immunodiffusion (AGID) tests: a native haptene (NH) from Brucella melitensis 16M, and a polysaccharide (PS) from B. abortus 1119-3, both obtained by non-hydrolytic methods, and the (O-Chain) polysaccharide extracted also from B. abortus 1119-3 but using an hydrolytic method. Three groups of bovine sera were tested: a) Naturally infected (n = 76); b) Non-infected (n = 130) and c) S-19 vaccinated (n = 61); the sensitivity (Se), the specificity (Sp) and the ability to differentiate vaccinated (ADV) were determined in each group a, b and c respectively. The highest Se in the RID test (84.3%) was achieved by NH; while the three antigens gave 100% Sp. The O-Chain showed 100% ADV in this test. In the AGID test PS antigen showed the best Se (86.6%), and all antigens showed 100% of Sp and ADV. Finally, for its production qualities and efficiency the antigens PS and NH represent a promising alternative for complementary diagnosis of brucellosis. |
publishDate |
1999 |
dc.date.none.fl_str_mv |
1999-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/5753 10.1590/S1413-95961999000300002 |
url |
https://www.revistas.usp.br/bjvras/article/view/5753 |
identifier_str_mv |
10.1590/S1413-95961999000300002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/5753/64776 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 36 Núm. 3 (1999); 116-120 Brazilian Journal of Veterinary Research and Animal Science; Vol. 36 No. 3 (1999); 116-120 Brazilian Journal of Veterinary Research and Animal Science; v. 36 n. 3 (1999); 116-120 Brazilian Journal of Veterinary Research and Animal Science; V. 36 N. 3 (1999); 116-120 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051554701770752 |