Expression of progranulin during the first stages of liver development in rat Fischer 344
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/74680 |
Resumo: | Transplants are the only effective therapy for the treatment of advanced liver diseases such as cirrhosis. Given the limited number of organ donors, regenerative medicine has sought for sources of cells and tissues for replacement therapy. Embryonic stem cells are a promising source of material for transplantation because of their exclusive property of being expanded indefinitely in culture, thus, they are a source of replacement tissue. Moreover, they are capable of differentiating into practically all cell types, and may be utilized in replacement therapy in various diseases. The liver bud has bipotent stem cells that have not yet differentiated into hepatocytes or biliary duct cells; however, they have great potential of proliferation and differentiation. Thus, the challenge is to identify methods that promote their differentiation in specific and functional strains. This study aimed to evaluate the role of the progranulin growth factor PGRN during the liver development of rats F344, since this growth factor could be utilized in protocols of differentiation of stem cells of the liver bud in functional hepatocytes. The results showed that PGRN is present during different periods of hepatogenesis in F344 rats, and that this growth factor should be involved in the process of differentiation of hepatoblasts into hepatocytes after activation by HNF4α , however, PGRN seems not to exert a cellular proliferation function during the hepatogenesis. Thus, PGRN can be used in future protocols of liver cell differentiation directed toward cellular therapy in Regenerative Medicine. |
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Brazilian Journal of Veterinary Research and Animal Science |
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Expression of progranulin during the first stages of liver development in rat Fischer 344Expressão da progranulina durante os primeiros estágios de desenvolvimento hepático em ratos Fischer 344ProgranulinaCélulas-tronco embrionáriasHNF4αBroto hepáticoPCNAProgranulinEmbryonic stem cellsHNF4αLiver budPCNA.Transplants are the only effective therapy for the treatment of advanced liver diseases such as cirrhosis. Given the limited number of organ donors, regenerative medicine has sought for sources of cells and tissues for replacement therapy. Embryonic stem cells are a promising source of material for transplantation because of their exclusive property of being expanded indefinitely in culture, thus, they are a source of replacement tissue. Moreover, they are capable of differentiating into practically all cell types, and may be utilized in replacement therapy in various diseases. The liver bud has bipotent stem cells that have not yet differentiated into hepatocytes or biliary duct cells; however, they have great potential of proliferation and differentiation. Thus, the challenge is to identify methods that promote their differentiation in specific and functional strains. This study aimed to evaluate the role of the progranulin growth factor PGRN during the liver development of rats F344, since this growth factor could be utilized in protocols of differentiation of stem cells of the liver bud in functional hepatocytes. The results showed that PGRN is present during different periods of hepatogenesis in F344 rats, and that this growth factor should be involved in the process of differentiation of hepatoblasts into hepatocytes after activation by HNF4α , however, PGRN seems not to exert a cellular proliferation function during the hepatogenesis. Thus, PGRN can be used in future protocols of liver cell differentiation directed toward cellular therapy in Regenerative Medicine.Os transplantes são a única terapia eficaz para o tratamento de doenças hepáticas avançadas, como a cirrose. Dado o número limitado de doadores de órgãos, a medicina regenerativa tem procurado fontes de células para a terapia de substituição. As células embrionárias são uma fonte promissora de material para o transplante devido à sua propriedade exclusiva de ser expandida indefinidamente em cultura, assim, elas são uma fonte de tecido de substituição. Além disso, são capazes de se diferenciar em praticamente todos os tipos celulares, e podem ser utilizadas na terapia de substituição em várias doenças. O broto hepático tem células-tronco (CT) bipotenciais que ainda não se diferenciam em hepatócitos ou células do ducto biliar, contudo, elas têm um grande potencial de proliferação e de diferenciação. Desse modo, o desafio é identificar métodos que promovam sua diferenciação em linhagens específicas e funcionais. Este estudo teve como objetivo avaliar o papel do fator de crescimento progranulina (PGRN) durante o desenvolvimento hepático em ratos F344, uma vez que a PGRN poderia ser utilizada em protocolos de diferenciação de CT do broto hepático em hepatócitos funcionais. Os resultados mostraram que PGRN está presente durante diferentes períodos da hepatogênese em ratos F344, e que a mesma deve estar envolvida no processo de diferenciação de hepatoblastos em hepatócitos após ativação por HNF4α, no entanto, a PGRN parece não desempenhar uma função de proliferação celular durante a hepatogênese. Assim, a PGRN pode ser usada em futuros protocolos de diferenciação de células hepáticas voltadas para a terapia celular na medicina regenerativa.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2013-08-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/7468010.11606/issn.2318-3659.v50i4p270-278Brazilian Journal of Veterinary Research and Animal Science; Vol. 50 Núm. 4 (2013); 270-278Brazilian Journal of Veterinary Research and Animal Science; Vol. 50 No. 4 (2013); 270-278Brazilian Journal of Veterinary Research and Animal Science; v. 50 n. 4 (2013); 270-278Brazilian Journal of Veterinary Research and Animal Science; V. 50 N. 4 (2013); 270-2781678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/74680/79054Lima, Arthur C. de L.Passos, Cristiane C.Ferreira, Amanda O.Bateman, AndrewMiglino, Maria A.Guerra, Ricardo R.info:eu-repo/semantics/openAccess2020-06-23T04:07:06Zoai:revistas.usp.br:article/74680Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:50.892706Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Expression of progranulin during the first stages of liver development in rat Fischer 344 Expressão da progranulina durante os primeiros estágios de desenvolvimento hepático em ratos Fischer 344 |
title |
Expression of progranulin during the first stages of liver development in rat Fischer 344 |
spellingShingle |
Expression of progranulin during the first stages of liver development in rat Fischer 344 Lima, Arthur C. de L. Progranulina Células-tronco embrionárias HNF4α Broto hepático PCNA Progranulin Embryonic stem cells HNF4α Liver bud PCNA. |
title_short |
Expression of progranulin during the first stages of liver development in rat Fischer 344 |
title_full |
Expression of progranulin during the first stages of liver development in rat Fischer 344 |
title_fullStr |
Expression of progranulin during the first stages of liver development in rat Fischer 344 |
title_full_unstemmed |
Expression of progranulin during the first stages of liver development in rat Fischer 344 |
title_sort |
Expression of progranulin during the first stages of liver development in rat Fischer 344 |
author |
Lima, Arthur C. de L. |
author_facet |
Lima, Arthur C. de L. Passos, Cristiane C. Ferreira, Amanda O. Bateman, Andrew Miglino, Maria A. Guerra, Ricardo R. |
author_role |
author |
author2 |
Passos, Cristiane C. Ferreira, Amanda O. Bateman, Andrew Miglino, Maria A. Guerra, Ricardo R. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Lima, Arthur C. de L. Passos, Cristiane C. Ferreira, Amanda O. Bateman, Andrew Miglino, Maria A. Guerra, Ricardo R. |
dc.subject.por.fl_str_mv |
Progranulina Células-tronco embrionárias HNF4α Broto hepático PCNA Progranulin Embryonic stem cells HNF4α Liver bud PCNA. |
topic |
Progranulina Células-tronco embrionárias HNF4α Broto hepático PCNA Progranulin Embryonic stem cells HNF4α Liver bud PCNA. |
description |
Transplants are the only effective therapy for the treatment of advanced liver diseases such as cirrhosis. Given the limited number of organ donors, regenerative medicine has sought for sources of cells and tissues for replacement therapy. Embryonic stem cells are a promising source of material for transplantation because of their exclusive property of being expanded indefinitely in culture, thus, they are a source of replacement tissue. Moreover, they are capable of differentiating into practically all cell types, and may be utilized in replacement therapy in various diseases. The liver bud has bipotent stem cells that have not yet differentiated into hepatocytes or biliary duct cells; however, they have great potential of proliferation and differentiation. Thus, the challenge is to identify methods that promote their differentiation in specific and functional strains. This study aimed to evaluate the role of the progranulin growth factor PGRN during the liver development of rats F344, since this growth factor could be utilized in protocols of differentiation of stem cells of the liver bud in functional hepatocytes. The results showed that PGRN is present during different periods of hepatogenesis in F344 rats, and that this growth factor should be involved in the process of differentiation of hepatoblasts into hepatocytes after activation by HNF4α , however, PGRN seems not to exert a cellular proliferation function during the hepatogenesis. Thus, PGRN can be used in future protocols of liver cell differentiation directed toward cellular therapy in Regenerative Medicine. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-08-17 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/74680 10.11606/issn.2318-3659.v50i4p270-278 |
url |
https://www.revistas.usp.br/bjvras/article/view/74680 |
identifier_str_mv |
10.11606/issn.2318-3659.v50i4p270-278 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/74680/79054 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 50 Núm. 4 (2013); 270-278 Brazilian Journal of Veterinary Research and Animal Science; Vol. 50 No. 4 (2013); 270-278 Brazilian Journal of Veterinary Research and Animal Science; v. 50 n. 4 (2013); 270-278 Brazilian Journal of Veterinary Research and Animal Science; V. 50 N. 4 (2013); 270-278 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
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1797051566469939200 |