Use of therapeutic antirabic serum in the production of conjugate for the direct immunofluorescence of rabies
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 1991 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
| DOI: | 10.11606/issn.1678-4456.bjvras.1991.51945 |
| Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/51945 |
Resumo: | A procedure for the production of antirabies fluorescent antibody conjugate using equine therapeutic hyperimmune serum is described. The IgG fraction is rapid and easily purified and after labelled with the fluorochrome. By this procedure, laboratories without facilities to take up the work of raising antirabic serum can obtain high quality conjugates. |
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Brazilian Journal of Veterinary Research and Animal Science |
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Use of therapeutic antirabic serum in the production of conjugate for the direct immunofluorescence of rabiesEmprego de soro anti-rábico terapêutico na produção de conjugado para a reação de imunofluorescência na raivaRaiva (diagnóstico)ImunofluorescênciaSoros imunes (soro hiperimune de equino)IgG (purificação)Soro anti-rábico terapêuticoRabies (diagnosis)Fluorescent antibody techniqueImmune sera (equine hyperimmune serum)IgG (purification)Therapeutic antirabic serumA procedure for the production of antirabies fluorescent antibody conjugate using equine therapeutic hyperimmune serum is described. The IgG fraction is rapid and easily purified and after labelled with the fluorochrome. By this procedure, laboratories without facilities to take up the work of raising antirabic serum can obtain high quality conjugates.Uma metodologia de produção de conjugado imunofluorescente anti-rábico a partir de soro hiperimune equino para fins terapêuticos é descrita. A fração IgG do soro é purificada de forma simples e rápida e posteriormente marcada com o fluoro cromo. Através dessa metodologia, laboratórios que não disponham de infraestrutura para produção de soro hiperimune anti-rábico podem obter conjugados de boa qualidade.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia1991-12-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/5194510.11606/issn.1678-4456.bjvras.1991.51945Brazilian Journal of Veterinary Research and Animal Science; Vol. 28 Núm. 2 (1991); 249-250Brazilian Journal of Veterinary Research and Animal Science; Vol. 28 No. 2 (1991); 249-250Brazilian Journal of Veterinary Research and Animal Science; v. 28 n. 2 (1991); 249-250Brazilian Journal of Veterinary Research and Animal Science; V. 28 N. 2 (1991); 249-2501678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/51945/55996Richtzenhain, Leonardo JoséIto, Fumio HonmaJerez, José AntonioMavatari, Ana Rita YumiAlbas, Avelinoinfo:eu-repo/semantics/openAccess2020-06-23T04:56:14Zoai:revistas.usp.br:article/51945Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:23.882228Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
| dc.title.none.fl_str_mv |
Use of therapeutic antirabic serum in the production of conjugate for the direct immunofluorescence of rabies Emprego de soro anti-rábico terapêutico na produção de conjugado para a reação de imunofluorescência na raiva |
| title |
Use of therapeutic antirabic serum in the production of conjugate for the direct immunofluorescence of rabies |
| spellingShingle |
Use of therapeutic antirabic serum in the production of conjugate for the direct immunofluorescence of rabies Use of therapeutic antirabic serum in the production of conjugate for the direct immunofluorescence of rabies Richtzenhain, Leonardo José Raiva (diagnóstico) Imunofluorescência Soros imunes (soro hiperimune de equino) IgG (purificação) Soro anti-rábico terapêutico Rabies (diagnosis) Fluorescent antibody technique Immune sera (equine hyperimmune serum) IgG (purification) Therapeutic antirabic serum Richtzenhain, Leonardo José Raiva (diagnóstico) Imunofluorescência Soros imunes (soro hiperimune de equino) IgG (purificação) Soro anti-rábico terapêutico Rabies (diagnosis) Fluorescent antibody technique Immune sera (equine hyperimmune serum) IgG (purification) Therapeutic antirabic serum |
| title_short |
Use of therapeutic antirabic serum in the production of conjugate for the direct immunofluorescence of rabies |
| title_full |
Use of therapeutic antirabic serum in the production of conjugate for the direct immunofluorescence of rabies |
| title_fullStr |
Use of therapeutic antirabic serum in the production of conjugate for the direct immunofluorescence of rabies Use of therapeutic antirabic serum in the production of conjugate for the direct immunofluorescence of rabies |
| title_full_unstemmed |
Use of therapeutic antirabic serum in the production of conjugate for the direct immunofluorescence of rabies Use of therapeutic antirabic serum in the production of conjugate for the direct immunofluorescence of rabies |
| title_sort |
Use of therapeutic antirabic serum in the production of conjugate for the direct immunofluorescence of rabies |
| author |
Richtzenhain, Leonardo José |
| author_facet |
Richtzenhain, Leonardo José Richtzenhain, Leonardo José Ito, Fumio Honma Jerez, José Antonio Mavatari, Ana Rita Yumi Albas, Avelino Ito, Fumio Honma Jerez, José Antonio Mavatari, Ana Rita Yumi Albas, Avelino |
| author_role |
author |
| author2 |
Ito, Fumio Honma Jerez, José Antonio Mavatari, Ana Rita Yumi Albas, Avelino |
| author2_role |
author author author author |
| dc.contributor.author.fl_str_mv |
Richtzenhain, Leonardo José Ito, Fumio Honma Jerez, José Antonio Mavatari, Ana Rita Yumi Albas, Avelino |
| dc.subject.por.fl_str_mv |
Raiva (diagnóstico) Imunofluorescência Soros imunes (soro hiperimune de equino) IgG (purificação) Soro anti-rábico terapêutico Rabies (diagnosis) Fluorescent antibody technique Immune sera (equine hyperimmune serum) IgG (purification) Therapeutic antirabic serum |
| topic |
Raiva (diagnóstico) Imunofluorescência Soros imunes (soro hiperimune de equino) IgG (purificação) Soro anti-rábico terapêutico Rabies (diagnosis) Fluorescent antibody technique Immune sera (equine hyperimmune serum) IgG (purification) Therapeutic antirabic serum |
| description |
A procedure for the production of antirabies fluorescent antibody conjugate using equine therapeutic hyperimmune serum is described. The IgG fraction is rapid and easily purified and after labelled with the fluorochrome. By this procedure, laboratories without facilities to take up the work of raising antirabic serum can obtain high quality conjugates. |
| publishDate |
1991 |
| dc.date.none.fl_str_mv |
1991-12-02 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/51945 10.11606/issn.1678-4456.bjvras.1991.51945 |
| url |
https://www.revistas.usp.br/bjvras/article/view/51945 |
| identifier_str_mv |
10.11606/issn.1678-4456.bjvras.1991.51945 |
| dc.language.iso.fl_str_mv |
por |
| language |
por |
| dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/51945/55996 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
| publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
| dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 28 Núm. 2 (1991); 249-250 Brazilian Journal of Veterinary Research and Animal Science; Vol. 28 No. 2 (1991); 249-250 Brazilian Journal of Veterinary Research and Animal Science; v. 28 n. 2 (1991); 249-250 Brazilian Journal of Veterinary Research and Animal Science; V. 28 N. 2 (1991); 249-250 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
| instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
| instacron_str |
USP |
| institution |
USP |
| reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
| collection |
Brazilian Journal of Veterinary Research and Animal Science |
| repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
| repository.mail.fl_str_mv |
bjvras@usp.br |
| _version_ |
1822182148723965952 |
| dc.identifier.doi.none.fl_str_mv |
10.11606/issn.1678-4456.bjvras.1991.51945 |