Comparação da eficácia de dois métodos de extração para determinação do perfil lipídico de ácidos graxos do sêmen ovino por meio da Cromatografia Líquida de Alta Eficiência
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/34356 |
Resumo: | The sheep industry for wool, milk and meat production is of increasing importance and new technologies for assessment of semen are in course for the elucidation of male infertility causes. It is well known that damage to the sperm membrane decreases semen quality. Considering that sperm membranes are composed by a phospholipid bilayer, and that lipid peroxidation is a major cause of cell damage, studieson the lipid components of semen are relevant. Lipid peroxidation is a consequence of the reaction between lipids and reactive oxygen species. This event may be reduced in the presence of antioxidants in semen. Semen was collected with an artificial vagina and, after sperm evaluation, samples were centrifuged to separate the sample into two fractions: seminal plasma and spermatozoa pellet. Both had their lipids extracted by two different methods based on Folch, Less& Stanley method modified, using chloroform e methanol as solvents. After the extraction, some esterified fatty acids were qualified and quantified for the sensitivity and specificity to the high performance liquid chromatography in order to determine the most efficient extraction technique in quantitative and qualitative aspects. Statistical analysis was performed using SAS software for Windows. The predominant saturated fatty acid in sperm under these experimental conditions was the myristic and the most abundant insaturated fatty acid in both extractions was DHA. In seminal plasma, in both methods, the prevailing fatty acid is the saturated palmitic and the unsaturated oleic. Among the methods evaluated, we obtained the best results of identification and quantification of fatty acids in Method 1. |
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Brazilian Journal of Veterinary Research and Animal Science |
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Comparação da eficácia de dois métodos de extração para determinação do perfil lipídico de ácidos graxos do sêmen ovino por meio da Cromatografia Líquida de Alta EficiênciaComparison of the efficacy of two extraction methods for determination of lipid fatty acids of sheep semen by high performance liquid chromatographySêmenOvinoPerfil lipídioHPLCExtraçãoSemenSheepLipid profileHPLCExtractionThe sheep industry for wool, milk and meat production is of increasing importance and new technologies for assessment of semen are in course for the elucidation of male infertility causes. It is well known that damage to the sperm membrane decreases semen quality. Considering that sperm membranes are composed by a phospholipid bilayer, and that lipid peroxidation is a major cause of cell damage, studieson the lipid components of semen are relevant. Lipid peroxidation is a consequence of the reaction between lipids and reactive oxygen species. This event may be reduced in the presence of antioxidants in semen. Semen was collected with an artificial vagina and, after sperm evaluation, samples were centrifuged to separate the sample into two fractions: seminal plasma and spermatozoa pellet. Both had their lipids extracted by two different methods based on Folch, Less& Stanley method modified, using chloroform e methanol as solvents. After the extraction, some esterified fatty acids were qualified and quantified for the sensitivity and specificity to the high performance liquid chromatography in order to determine the most efficient extraction technique in quantitative and qualitative aspects. Statistical analysis was performed using SAS software for Windows. The predominant saturated fatty acid in sperm under these experimental conditions was the myristic and the most abundant insaturated fatty acid in both extractions was DHA. In seminal plasma, in both methods, the prevailing fatty acid is the saturated palmitic and the unsaturated oleic. Among the methods evaluated, we obtained the best results of identification and quantification of fatty acids in Method 1.A indústria de lã, leite, carne e pele de ovinos tem crescido em importância e novas biotecnologias do sêmen estão sendo estudadas para a elucidação de causas de infertilidade em machos. Sabe-se que danos causados na membrana espermática diminuem a qualidade seminal. Considerando que estas são compostas por uma bicamada de fosfolipídios e que a peroxidação lipídica é causadora de lesão celular, explica-se a importância de estudos sobre os lipídios constituintes do sêmen. A peroxidação lipídica é consequente da reação entre os lipídios e as espécies reativas de oxigênio. Esse quadro pode ser controlado pela presença de antioxidantes no sêmen. O sêmen foi coletado pela técnica da vagina artificial e após análise imediata e mediata, foi separado em duas frações: plasma seminal e pellet de espermatozoide. Seus lipídios foram extraídos por dois métodosbaseados no método Folch, Lesse Stanley modificado diferentes, utilizando clorofórmio e metanol como solventes. Após foram qualificados e quantificados pela especificidade e sensibilidade da Cromatografia Liquida de Alta Eficiência e escolheu-se a melhor extração. A análise estatística dos dados foi realizada através do programa SAS for Windows. O AG saturado predominante no espermatozoide é o mirístico e o AG insaturado predominante é o DHA, em ambas as extrações. No plasma seminal, nos dois métodos, o AG saturado que prevalece é o palmítico e o insaturado é o oleico. Dentre os dois métodos estudados, o que obtivemos melhores resultados na identificação e quantificação dos AG foi a Método 1.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/3435610.11606/S1413-95962011000600007Brazilian Journal of Veterinary Research and Animal Science; Vol. 48 Núm. 6 (2011); 486-494Brazilian Journal of Veterinary Research and Animal Science; Vol. 48 No. 6 (2011); 486-494Brazilian Journal of Veterinary Research and Animal Science; v. 48 n. 6 (2011); 486-494Brazilian Journal of Veterinary Research and Animal Science; V. 48 N. 6 (2011); 486-4941678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/34356/37094Cardoso, Patrícia Barbosa SallaNichi, MarcílioZogno, Maria AméliaFujiwara, HaroldoBarnabe, Valquíria HyppolitoBarnabe, Renato Camparanutinfo:eu-repo/semantics/openAccess2020-06-23T04:08:52Zoai:revistas.usp.br:article/34356Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:08.855729Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Comparação da eficácia de dois métodos de extração para determinação do perfil lipídico de ácidos graxos do sêmen ovino por meio da Cromatografia Líquida de Alta Eficiência Comparison of the efficacy of two extraction methods for determination of lipid fatty acids of sheep semen by high performance liquid chromatography |
title |
Comparação da eficácia de dois métodos de extração para determinação do perfil lipídico de ácidos graxos do sêmen ovino por meio da Cromatografia Líquida de Alta Eficiência |
spellingShingle |
Comparação da eficácia de dois métodos de extração para determinação do perfil lipídico de ácidos graxos do sêmen ovino por meio da Cromatografia Líquida de Alta Eficiência Cardoso, Patrícia Barbosa Salla Sêmen Ovino Perfil lipídio HPLC Extração Semen Sheep Lipid profile HPLC Extraction |
title_short |
Comparação da eficácia de dois métodos de extração para determinação do perfil lipídico de ácidos graxos do sêmen ovino por meio da Cromatografia Líquida de Alta Eficiência |
title_full |
Comparação da eficácia de dois métodos de extração para determinação do perfil lipídico de ácidos graxos do sêmen ovino por meio da Cromatografia Líquida de Alta Eficiência |
title_fullStr |
Comparação da eficácia de dois métodos de extração para determinação do perfil lipídico de ácidos graxos do sêmen ovino por meio da Cromatografia Líquida de Alta Eficiência |
title_full_unstemmed |
Comparação da eficácia de dois métodos de extração para determinação do perfil lipídico de ácidos graxos do sêmen ovino por meio da Cromatografia Líquida de Alta Eficiência |
title_sort |
Comparação da eficácia de dois métodos de extração para determinação do perfil lipídico de ácidos graxos do sêmen ovino por meio da Cromatografia Líquida de Alta Eficiência |
author |
Cardoso, Patrícia Barbosa Salla |
author_facet |
Cardoso, Patrícia Barbosa Salla Nichi, Marcílio Zogno, Maria Amélia Fujiwara, Haroldo Barnabe, Valquíria Hyppolito Barnabe, Renato Camparanut |
author_role |
author |
author2 |
Nichi, Marcílio Zogno, Maria Amélia Fujiwara, Haroldo Barnabe, Valquíria Hyppolito Barnabe, Renato Camparanut |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Cardoso, Patrícia Barbosa Salla Nichi, Marcílio Zogno, Maria Amélia Fujiwara, Haroldo Barnabe, Valquíria Hyppolito Barnabe, Renato Camparanut |
dc.subject.por.fl_str_mv |
Sêmen Ovino Perfil lipídio HPLC Extração Semen Sheep Lipid profile HPLC Extraction |
topic |
Sêmen Ovino Perfil lipídio HPLC Extração Semen Sheep Lipid profile HPLC Extraction |
description |
The sheep industry for wool, milk and meat production is of increasing importance and new technologies for assessment of semen are in course for the elucidation of male infertility causes. It is well known that damage to the sperm membrane decreases semen quality. Considering that sperm membranes are composed by a phospholipid bilayer, and that lipid peroxidation is a major cause of cell damage, studieson the lipid components of semen are relevant. Lipid peroxidation is a consequence of the reaction between lipids and reactive oxygen species. This event may be reduced in the presence of antioxidants in semen. Semen was collected with an artificial vagina and, after sperm evaluation, samples were centrifuged to separate the sample into two fractions: seminal plasma and spermatozoa pellet. Both had their lipids extracted by two different methods based on Folch, Less& Stanley method modified, using chloroform e methanol as solvents. After the extraction, some esterified fatty acids were qualified and quantified for the sensitivity and specificity to the high performance liquid chromatography in order to determine the most efficient extraction technique in quantitative and qualitative aspects. Statistical analysis was performed using SAS software for Windows. The predominant saturated fatty acid in sperm under these experimental conditions was the myristic and the most abundant insaturated fatty acid in both extractions was DHA. In seminal plasma, in both methods, the prevailing fatty acid is the saturated palmitic and the unsaturated oleic. Among the methods evaluated, we obtained the best results of identification and quantification of fatty acids in Method 1. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/34356 10.11606/S1413-95962011000600007 |
url |
https://www.revistas.usp.br/bjvras/article/view/34356 |
identifier_str_mv |
10.11606/S1413-95962011000600007 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/34356/37094 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 48 Núm. 6 (2011); 486-494 Brazilian Journal of Veterinary Research and Animal Science; Vol. 48 No. 6 (2011); 486-494 Brazilian Journal of Veterinary Research and Animal Science; v. 48 n. 6 (2011); 486-494 Brazilian Journal of Veterinary Research and Animal Science; V. 48 N. 6 (2011); 486-494 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051559957233664 |