Comparison of five extenders for canine semen freezing

Detalhes bibliográficos
Autor(a) principal: Santos, Silvia Edelweiss Crusco dos
Data de Publicação: 1999
Outros Autores: Vannuchi, Camila Infantosi, Satzinger, Sandra, Assumpção, Mayra Elena Ortiz D’Avila, Visintin, José Antonio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
Texto Completo: https://www.revistas.usp.br/bjvras/article/view/5735
Resumo: Semen was collected from six stud dogs to compare five extenders in the semen freezing process. Each ejaculate was divided in five parts and added to tris-fructose-citric acid, glicine, lactose, skim milk and tris-fructose-citrate extenders. The semen was diluted at 37°C in extenders without glycerol, in the ratio 1:1 and cooled for 60 minutes to reach a 5°C temperature. Then, extenders with glycerol in the ratio of 2:1 were added to give the final prefreezing concentration of 4% of glycerol. The diluted semen with the cryoprotectant was maintained for a further 60 minutes in refrigeration to equilibrate the spermatozoa in the glycerol and packaged in 0.5 ml plastic straws. The straws were maintained for 30 minutes in vapor, plunged and stored in liquid nitrogen. Sperm morphology was evaluated before and after freezing, whereas progressive motility (%) and velocity of forward progression (0-5) were appraised in different periods of the freezing process. The extender tris-fructose-citric acid showed the best post thaw progressive motility and velocity of forward progression compared to the others extenders. Semen freezing increased major sperm morphological abnormalities, regardless of the extender.
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spelling Comparison of five extenders for canine semen freezingComparação entre cinco diluidores na congelação de sêmen de cãesDiluentesCongelamentoSemenCdes.DiluentsFreezingSemenDogsSemen was collected from six stud dogs to compare five extenders in the semen freezing process. Each ejaculate was divided in five parts and added to tris-fructose-citric acid, glicine, lactose, skim milk and tris-fructose-citrate extenders. The semen was diluted at 37°C in extenders without glycerol, in the ratio 1:1 and cooled for 60 minutes to reach a 5°C temperature. Then, extenders with glycerol in the ratio of 2:1 were added to give the final prefreezing concentration of 4% of glycerol. The diluted semen with the cryoprotectant was maintained for a further 60 minutes in refrigeration to equilibrate the spermatozoa in the glycerol and packaged in 0.5 ml plastic straws. The straws were maintained for 30 minutes in vapor, plunged and stored in liquid nitrogen. Sperm morphology was evaluated before and after freezing, whereas progressive motility (%) and velocity of forward progression (0-5) were appraised in different periods of the freezing process. The extender tris-fructose-citric acid showed the best post thaw progressive motility and velocity of forward progression compared to the others extenders. Semen freezing increased major sperm morphological abnormalities, regardless of the extender.Foram utilizados ejaculados de 6 cães para comparar cinco diluidores no processo de congelação de sêmen. Cada ejaculado foi dividido em 5 partes e adicionadas aos diluidores tris-fructose-ácido cítrico, glicina, lactose, leite desnatado e tris-fructose-ácido cítrico. O sêmen foi diluído a 37°C sem adição de glicerol na proporção 1:1 (fração A) e refrigerado durante 60 minutos até atingir a temperatura de 5ºC, quando foi adicionada a fração dos diluidores contendo glicerol (fração B) na proporção 2:1, atingindo a concentração final de 4% de glicerol. O sêmen diluído permaneceu por 60 minutos em refrigeração para equilíbrio no glicerol, sendo envasado em palhetas de 0,5 ml, mantido por 30 minutos no vapor de nitrogênio e imerso e armazenado em nitrogênio líquido. Foram avaliados a motilidade progressiva retilínea, o vigor e os defeitos espermáticos antes da congelação e após a descongelação do sêmen em água a 37°C. Os resultados mostraram que os diluidores tris-fructose-ácido cítrico e glicina apresentaram as melhores médias de motilidade progressiva retilínea e de vigor espermático após a descongelação. A congelação aumentou a freqüência dos defeitos espermáticos maiores independente do diluidor.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia1999-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/573510.1590/S1413-95961999000500004Brazilian Journal of Veterinary Research and Animal Science; Vol. 36 Núm. 5 (1999); 244-249Brazilian Journal of Veterinary Research and Animal Science; Vol. 36 No. 5 (1999); 244-249Brazilian Journal of Veterinary Research and Animal Science; v. 36 n. 5 (1999); 244-249Brazilian Journal of Veterinary Research and Animal Science; V. 36 N. 5 (1999); 244-2491678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/5735/64798Santos, Silvia Edelweiss Crusco dosVannuchi, Camila InfantosiSatzinger, SandraAssumpção, Mayra Elena Ortiz D’AvilaVisintin, José Antonioinfo:eu-repo/semantics/openAccess2020-06-23T04:44:21Zoai:revistas.usp.br:article/5735Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:19.768063Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv Comparison of five extenders for canine semen freezing
Comparação entre cinco diluidores na congelação de sêmen de cães
title Comparison of five extenders for canine semen freezing
spellingShingle Comparison of five extenders for canine semen freezing
Santos, Silvia Edelweiss Crusco dos
Diluentes
Congelamento
Semen
Cdes.
Diluents
Freezing
Semen
Dogs
title_short Comparison of five extenders for canine semen freezing
title_full Comparison of five extenders for canine semen freezing
title_fullStr Comparison of five extenders for canine semen freezing
title_full_unstemmed Comparison of five extenders for canine semen freezing
title_sort Comparison of five extenders for canine semen freezing
author Santos, Silvia Edelweiss Crusco dos
author_facet Santos, Silvia Edelweiss Crusco dos
Vannuchi, Camila Infantosi
Satzinger, Sandra
Assumpção, Mayra Elena Ortiz D’Avila
Visintin, José Antonio
author_role author
author2 Vannuchi, Camila Infantosi
Satzinger, Sandra
Assumpção, Mayra Elena Ortiz D’Avila
Visintin, José Antonio
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Santos, Silvia Edelweiss Crusco dos
Vannuchi, Camila Infantosi
Satzinger, Sandra
Assumpção, Mayra Elena Ortiz D’Avila
Visintin, José Antonio
dc.subject.por.fl_str_mv Diluentes
Congelamento
Semen
Cdes.
Diluents
Freezing
Semen
Dogs
topic Diluentes
Congelamento
Semen
Cdes.
Diluents
Freezing
Semen
Dogs
description Semen was collected from six stud dogs to compare five extenders in the semen freezing process. Each ejaculate was divided in five parts and added to tris-fructose-citric acid, glicine, lactose, skim milk and tris-fructose-citrate extenders. The semen was diluted at 37°C in extenders without glycerol, in the ratio 1:1 and cooled for 60 minutes to reach a 5°C temperature. Then, extenders with glycerol in the ratio of 2:1 were added to give the final prefreezing concentration of 4% of glycerol. The diluted semen with the cryoprotectant was maintained for a further 60 minutes in refrigeration to equilibrate the spermatozoa in the glycerol and packaged in 0.5 ml plastic straws. The straws were maintained for 30 minutes in vapor, plunged and stored in liquid nitrogen. Sperm morphology was evaluated before and after freezing, whereas progressive motility (%) and velocity of forward progression (0-5) were appraised in different periods of the freezing process. The extender tris-fructose-citric acid showed the best post thaw progressive motility and velocity of forward progression compared to the others extenders. Semen freezing increased major sperm morphological abnormalities, regardless of the extender.
publishDate 1999
dc.date.none.fl_str_mv 1999-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/5735
10.1590/S1413-95961999000500004
url https://www.revistas.usp.br/bjvras/article/view/5735
identifier_str_mv 10.1590/S1413-95961999000500004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/5735/64798
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 36 Núm. 5 (1999); 244-249
Brazilian Journal of Veterinary Research and Animal Science; Vol. 36 No. 5 (1999); 244-249
Brazilian Journal of Veterinary Research and Animal Science; v. 36 n. 5 (1999); 244-249
Brazilian Journal of Veterinary Research and Animal Science; V. 36 N. 5 (1999); 244-249
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
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