Gene expression profile of Protamines and Transition Nuclear Proteins in bovine testis
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/74726 |
Resumo: | Protamines (PRM) are the major DNA-binding proteins in the sperm nucleus and can pack the DNA into less than 5% of the volume of a somatic cell nucleus. It is already known that bulls only have the PRM1 protein on mature spermatozoa while most mammals also have the PRM2. Transition nuclear proteins (Tnps) and PRMs are fundamental to DNA integrity. It has already been reported the influence of PRM on chromatin structures, generating low fertility. However, molecular mechanisms underlying these effects are not known. The relative expression of PRM1, PRM2, PRM3, Tnp1 and Tnp2 was determined by real time RT-PCR, using bovine specific primers and β-actin as endogenous control. Quantification of mRNA relative expression showed a higher expression of PRM1 compared to the other genes. The PRM3 mRNA had the lowest relative expression. A significant (p < 0.05) and positive correlation was found between PRM1 and PRM2 (r = 0.518), PRM2 and Tnp1 (r = 0.750), PRM2 and Tnp2 (r = 0.706), PRM3 and Tnp1 (r = 0.542), PRM3 and Tnp2 (r = 0.731) and between Tnp1 and Tnp2 (r = 0.820). Since most of the knowledge about protamine 2 in bovine is based on a work from 1990 and according to new studies we know that PRM1 and PRM2 are important to bull fertility, more research is needed to elucidate the real function of protamines on bovines. |
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oai:revistas.usp.br:article/74726 |
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USP-49 |
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Brazilian Journal of Veterinary Research and Animal Science |
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Gene expression profile of Protamines and Transition Nuclear Proteins in bovine testisExpressão gênica de protaminas e proteínas nucleares de transição em testículos bovinosTouroTestículoProtaminaRT-PCR em tempo realBullSpermatozoaProtamineReal time RT-PCRProtamines (PRM) are the major DNA-binding proteins in the sperm nucleus and can pack the DNA into less than 5% of the volume of a somatic cell nucleus. It is already known that bulls only have the PRM1 protein on mature spermatozoa while most mammals also have the PRM2. Transition nuclear proteins (Tnps) and PRMs are fundamental to DNA integrity. It has already been reported the influence of PRM on chromatin structures, generating low fertility. However, molecular mechanisms underlying these effects are not known. The relative expression of PRM1, PRM2, PRM3, Tnp1 and Tnp2 was determined by real time RT-PCR, using bovine specific primers and β-actin as endogenous control. Quantification of mRNA relative expression showed a higher expression of PRM1 compared to the other genes. The PRM3 mRNA had the lowest relative expression. A significant (p < 0.05) and positive correlation was found between PRM1 and PRM2 (r = 0.518), PRM2 and Tnp1 (r = 0.750), PRM2 and Tnp2 (r = 0.706), PRM3 and Tnp1 (r = 0.542), PRM3 and Tnp2 (r = 0.731) and between Tnp1 and Tnp2 (r = 0.820). Since most of the knowledge about protamine 2 in bovine is based on a work from 1990 and according to new studies we know that PRM1 and PRM2 are important to bull fertility, more research is needed to elucidate the real function of protamines on bovines.Protaminas (PRM) são as principais proteínas ligantes do DNA espermático e podem compactar o núcleo do espermatozoide em menos de 5% do volume de uma célula somática. Já se sabe que o touro produz apenas a PRM1 em espermatozoide maduro, enquanto a maioria dos mamíferos também produz a PRM2. As proteínas nucleares de transição (Tnps) e as PRMs são fundamentais para a integridade do DNA. Já foi descrita a influência das protaminas na estrutura da cromatina e a associação destas com a fertilidade. Entretanto, os mecanismos moleculares que geram mudanças na cromatina espermática são desconhecidos. A expressão relativa da PRM1, PRM2, PRM3, Tnp1 e Tnp2 foi determinada para dez testículos de touros oriundos de matadouros comerciais, utilizando a técnica de RT-PCR em tempo real, com primers específicos para bovinos e a β-actina como controle endógeno. Ao quantificar a expressão relativa do RNAm, detectou-se alta expressão relativa da PRM1, em comparação aos outros genes. A expressão relativa da PRM3 foi a menor de todos os genes. Foram encontradas correlações positivas e significantes (p < 0,05) entre PRM1 e PRM2 (r = 0,518), PRM2 e Tnp1 (r = 0,750), PRM2 e Tnp2 (r = 0,706), PRM3 e Tnp1 (r = 0,542), PRM3 e Tnp2 (r = 0,731) e entre Tnp1 e Tnp2 (r = 0,820). Visto que a maioria dos conhecimentos sobre a PRM2 estão baseados em um trabalho de 1990 e, de acordo com recentes estudos se sabe que a PRM1 e a PRM2 são importantes para a fertilidade do touro, mais estudos são necessários para determinar a real função das protaminas em touros.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2013-08-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/7472610.11606/issn.2318-3659.v50i4p316-322Brazilian Journal of Veterinary Research and Animal Science; Vol. 50 Núm. 4 (2013); 316-322Brazilian Journal of Veterinary Research and Animal Science; Vol. 50 No. 4 (2013); 316-322Brazilian Journal of Veterinary Research and Animal Science; v. 50 n. 4 (2013); 316-322Brazilian Journal of Veterinary Research and Animal Science; V. 50 N. 4 (2013); 316-3221678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/74726/79062Ferraz, Marcia de Almeida Monteiro MeloSimões, RenataBarros, Flávia de OliveiraMillazzoto, Marcella PercoraVisintin, José AntonioAssumpção, Mayra Elena Ortiz D’Ávilainfo:eu-repo/semantics/openAccess2020-06-23T04:07:00Zoai:revistas.usp.br:article/74726Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:51.136831Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Gene expression profile of Protamines and Transition Nuclear Proteins in bovine testis Expressão gênica de protaminas e proteínas nucleares de transição em testículos bovinos |
title |
Gene expression profile of Protamines and Transition Nuclear Proteins in bovine testis |
spellingShingle |
Gene expression profile of Protamines and Transition Nuclear Proteins in bovine testis Ferraz, Marcia de Almeida Monteiro Melo Touro Testículo Protamina RT-PCR em tempo real Bull Spermatozoa Protamine Real time RT-PCR |
title_short |
Gene expression profile of Protamines and Transition Nuclear Proteins in bovine testis |
title_full |
Gene expression profile of Protamines and Transition Nuclear Proteins in bovine testis |
title_fullStr |
Gene expression profile of Protamines and Transition Nuclear Proteins in bovine testis |
title_full_unstemmed |
Gene expression profile of Protamines and Transition Nuclear Proteins in bovine testis |
title_sort |
Gene expression profile of Protamines and Transition Nuclear Proteins in bovine testis |
author |
Ferraz, Marcia de Almeida Monteiro Melo |
author_facet |
Ferraz, Marcia de Almeida Monteiro Melo Simões, Renata Barros, Flávia de Oliveira Millazzoto, Marcella Percora Visintin, José Antonio Assumpção, Mayra Elena Ortiz D’Ávila |
author_role |
author |
author2 |
Simões, Renata Barros, Flávia de Oliveira Millazzoto, Marcella Percora Visintin, José Antonio Assumpção, Mayra Elena Ortiz D’Ávila |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Ferraz, Marcia de Almeida Monteiro Melo Simões, Renata Barros, Flávia de Oliveira Millazzoto, Marcella Percora Visintin, José Antonio Assumpção, Mayra Elena Ortiz D’Ávila |
dc.subject.por.fl_str_mv |
Touro Testículo Protamina RT-PCR em tempo real Bull Spermatozoa Protamine Real time RT-PCR |
topic |
Touro Testículo Protamina RT-PCR em tempo real Bull Spermatozoa Protamine Real time RT-PCR |
description |
Protamines (PRM) are the major DNA-binding proteins in the sperm nucleus and can pack the DNA into less than 5% of the volume of a somatic cell nucleus. It is already known that bulls only have the PRM1 protein on mature spermatozoa while most mammals also have the PRM2. Transition nuclear proteins (Tnps) and PRMs are fundamental to DNA integrity. It has already been reported the influence of PRM on chromatin structures, generating low fertility. However, molecular mechanisms underlying these effects are not known. The relative expression of PRM1, PRM2, PRM3, Tnp1 and Tnp2 was determined by real time RT-PCR, using bovine specific primers and β-actin as endogenous control. Quantification of mRNA relative expression showed a higher expression of PRM1 compared to the other genes. The PRM3 mRNA had the lowest relative expression. A significant (p < 0.05) and positive correlation was found between PRM1 and PRM2 (r = 0.518), PRM2 and Tnp1 (r = 0.750), PRM2 and Tnp2 (r = 0.706), PRM3 and Tnp1 (r = 0.542), PRM3 and Tnp2 (r = 0.731) and between Tnp1 and Tnp2 (r = 0.820). Since most of the knowledge about protamine 2 in bovine is based on a work from 1990 and according to new studies we know that PRM1 and PRM2 are important to bull fertility, more research is needed to elucidate the real function of protamines on bovines. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-08-17 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/74726 10.11606/issn.2318-3659.v50i4p316-322 |
url |
https://www.revistas.usp.br/bjvras/article/view/74726 |
identifier_str_mv |
10.11606/issn.2318-3659.v50i4p316-322 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/74726/79062 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 50 Núm. 4 (2013); 316-322 Brazilian Journal of Veterinary Research and Animal Science; Vol. 50 No. 4 (2013); 316-322 Brazilian Journal of Veterinary Research and Animal Science; v. 50 n. 4 (2013); 316-322 Brazilian Journal of Veterinary Research and Animal Science; V. 50 N. 4 (2013); 316-322 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051566484619264 |