A fast immunoassay for the screening of alfa-trenbolone in bovine urine

Detalhes bibliográficos
Autor(a) principal: Duarte, Keila Maria Roncato
Data de Publicação: 2006
Outros Autores: Gomes, Luiz Humberto, Paschoal, Jonas Augusto Rizzato, Meirelles, Cyro Ferreira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
Texto Completo: https://www.revistas.usp.br/bjvras/article/view/26538
Resumo: Brazil is one of the many countries that forbids the use of anabolic compounds, which generates difficulties on monitoring its use, once it has one of the biggest cattle herd. Therefore, several anabolic compounds are used, including trenbolone acetate. With the agreement of "Ministério da Agricultura, Pecuária e Abastecimento", an ELISA based test was done with the production of polyclonal antibodies in rabbits and testing in steers urine which received trenbolone acetate implants. The test showed to be cheap, easy and reliable to use in bovine urine to determine alfa- trenbolone, trenbolone acetate major metabolite, released in the animals urine until 60 days after implant was been injected. The results in comparison to the ones obtained by the commercial kit used by the Brazilian "Ministério da Agricultura, Pecuária e Abastecimento" were similar, with no significant differences.
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spelling A fast immunoassay for the screening of alfa-trenbolone in bovine urineUm imunoensaio rápido para a detecção de alfa-trembolona em urina bovinaTrembolonaELISABovinosTrenboloneELISACattleBrazil is one of the many countries that forbids the use of anabolic compounds, which generates difficulties on monitoring its use, once it has one of the biggest cattle herd. Therefore, several anabolic compounds are used, including trenbolone acetate. With the agreement of "Ministério da Agricultura, Pecuária e Abastecimento", an ELISA based test was done with the production of polyclonal antibodies in rabbits and testing in steers urine which received trenbolone acetate implants. The test showed to be cheap, easy and reliable to use in bovine urine to determine alfa- trenbolone, trenbolone acetate major metabolite, released in the animals urine until 60 days after implant was been injected. The results in comparison to the ones obtained by the commercial kit used by the Brazilian "Ministério da Agricultura, Pecuária e Abastecimento" were similar, with no significant differences.O Brasil está entre os países que mantêm o uso de anabolizantes proibido, o que gera dificuldades muito grandes no monitoramento destas substâncias, uma vez que possui o maior rebanho bovino. Contudo, diversas substâncias são largamente utilizadas, entre as quais o acetato de trembolona. Com o consentimento do Ministério da Agricultura, Pecuária e Abastecimento, foram produzidos anticorpos policlonais para um teste baseado em ELISA ("enzyme linked immunosorbent assay") capazes de detectar acetato de trembolona na urina de bovinos tratados com trembolona. O teste apresentou baixo custo e de fácil execução para a detecção de alfa-trembolone, o principal metabólito na absorção do acetato de trembolona, liberado na urina dos animais. Os resultados foram similares aos obtidos com o "kit" comercial usado pelo Ministério da Agricultura, Pecuária e Abastecimento do Brasil.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2006-12-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/2653810.11606/issn.1678-4456.bjvras.2006.26538Brazilian Journal of Veterinary Research and Animal Science; Vol. 43 (2006): Suplemento; 74-79Brazilian Journal of Veterinary Research and Animal Science; Vol. 43 (2006): Suplemento; 74-79Brazilian Journal of Veterinary Research and Animal Science; v. 43 (2006): Suplemento; 74-79Brazilian Journal of Veterinary Research and Animal Science; V. 43 (2006): Suplemento; 74-791678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/26538/28321Duarte, Keila Maria RoncatoGomes, Luiz HumbertoPaschoal, Jonas Augusto RizzatoMeirelles, Cyro Ferreirainfo:eu-repo/semantics/openAccess2020-06-23T04:16:01Zoai:revistas.usp.br:article/26538Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:48.943126Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv A fast immunoassay for the screening of alfa-trenbolone in bovine urine
Um imunoensaio rápido para a detecção de alfa-trembolona em urina bovina
title A fast immunoassay for the screening of alfa-trenbolone in bovine urine
spellingShingle A fast immunoassay for the screening of alfa-trenbolone in bovine urine
Duarte, Keila Maria Roncato
Trembolona
ELISA
Bovinos
Trenbolone
ELISA
Cattle
title_short A fast immunoassay for the screening of alfa-trenbolone in bovine urine
title_full A fast immunoassay for the screening of alfa-trenbolone in bovine urine
title_fullStr A fast immunoassay for the screening of alfa-trenbolone in bovine urine
title_full_unstemmed A fast immunoassay for the screening of alfa-trenbolone in bovine urine
title_sort A fast immunoassay for the screening of alfa-trenbolone in bovine urine
author Duarte, Keila Maria Roncato
author_facet Duarte, Keila Maria Roncato
Gomes, Luiz Humberto
Paschoal, Jonas Augusto Rizzato
Meirelles, Cyro Ferreira
author_role author
author2 Gomes, Luiz Humberto
Paschoal, Jonas Augusto Rizzato
Meirelles, Cyro Ferreira
author2_role author
author
author
dc.contributor.author.fl_str_mv Duarte, Keila Maria Roncato
Gomes, Luiz Humberto
Paschoal, Jonas Augusto Rizzato
Meirelles, Cyro Ferreira
dc.subject.por.fl_str_mv Trembolona
ELISA
Bovinos
Trenbolone
ELISA
Cattle
topic Trembolona
ELISA
Bovinos
Trenbolone
ELISA
Cattle
description Brazil is one of the many countries that forbids the use of anabolic compounds, which generates difficulties on monitoring its use, once it has one of the biggest cattle herd. Therefore, several anabolic compounds are used, including trenbolone acetate. With the agreement of "Ministério da Agricultura, Pecuária e Abastecimento", an ELISA based test was done with the production of polyclonal antibodies in rabbits and testing in steers urine which received trenbolone acetate implants. The test showed to be cheap, easy and reliable to use in bovine urine to determine alfa- trenbolone, trenbolone acetate major metabolite, released in the animals urine until 60 days after implant was been injected. The results in comparison to the ones obtained by the commercial kit used by the Brazilian "Ministério da Agricultura, Pecuária e Abastecimento" were similar, with no significant differences.
publishDate 2006
dc.date.none.fl_str_mv 2006-12-03
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/26538
10.11606/issn.1678-4456.bjvras.2006.26538
url https://www.revistas.usp.br/bjvras/article/view/26538
identifier_str_mv 10.11606/issn.1678-4456.bjvras.2006.26538
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/26538/28321
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 43 (2006): Suplemento; 74-79
Brazilian Journal of Veterinary Research and Animal Science; Vol. 43 (2006): Suplemento; 74-79
Brazilian Journal of Veterinary Research and Animal Science; v. 43 (2006): Suplemento; 74-79
Brazilian Journal of Veterinary Research and Animal Science; V. 43 (2006): Suplemento; 74-79
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
_version_ 1797051557842255872

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