Efeitos do fluoreto de sódio no epitélio da brânquia do peixe Guaru (Poecilia vivipara)
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/6288 |
Resumo: | Fluorine is an element much used by man and easily found in nature. Some studies have been carried out on the toxicity of fluorine and its cumulative effect on animal tissues. Sodium fluoride is described as being used in controlling the host of schistosomiasis (Biomphalaria glabrata). With a view to preserve the aquatic environment, it is proposed to verify the effect of sodium fluoride on fish (Poecilia vivipara). Ten fishes were divided into two groups. The first group was exposed to freshwater with sodium fluoride with a concentration of 5 ppm for a period of 21 days; the second group was exposed to freshwater. After 21 days the animals were sacrificed and their gills removed and processed according to histochemical/histologic methods and ultrastructural study. The gill leaflets were diaphanized in xylol. Histologic analyses using hematoxyline-eosin identified branchial epithelium and revealed mucous and chloride cells. The histochemical methods to detect glycoconjugate contents of mucous cells used: P.A.S, P.A.S. + salivar amylase; P.A.S. + acetylation ; P.A.S. + reversible acetylation; Alcian blue pH 2.5; Alcian blue pH 0.5; Alcian blue pH 2.5 + methylation; and Alcian blue pH 2.5 + reversible methylation. An increase in secretion of mucus and an alteration to the content of the granules were also observed, suggesting behavior changes of mucous type to enable these animals to adapt to the environment, thus altering the concentration of sodium fluoride. |
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USP-49 |
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Brazilian Journal of Veterinary Research and Animal Science |
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Efeitos do fluoreto de sódio no epitélio da brânquia do peixe Guaru (Poecilia vivipara)Effects of sodium fluoride in gill epithelium of Guppy fish (Poecilia vivipara)BrânquiasCélulas do cloroCélulas mucosasEsquistossomoseFluoreto de sódioChloride cellsFluorineGillsMucous cellsSchistosomiasisFluorine is an element much used by man and easily found in nature. Some studies have been carried out on the toxicity of fluorine and its cumulative effect on animal tissues. Sodium fluoride is described as being used in controlling the host of schistosomiasis (Biomphalaria glabrata). With a view to preserve the aquatic environment, it is proposed to verify the effect of sodium fluoride on fish (Poecilia vivipara). Ten fishes were divided into two groups. The first group was exposed to freshwater with sodium fluoride with a concentration of 5 ppm for a period of 21 days; the second group was exposed to freshwater. After 21 days the animals were sacrificed and their gills removed and processed according to histochemical/histologic methods and ultrastructural study. The gill leaflets were diaphanized in xylol. Histologic analyses using hematoxyline-eosin identified branchial epithelium and revealed mucous and chloride cells. The histochemical methods to detect glycoconjugate contents of mucous cells used: P.A.S, P.A.S. + salivar amylase; P.A.S. + acetylation ; P.A.S. + reversible acetylation; Alcian blue pH 2.5; Alcian blue pH 0.5; Alcian blue pH 2.5 + methylation; and Alcian blue pH 2.5 + reversible methylation. An increase in secretion of mucus and an alteration to the content of the granules were also observed, suggesting behavior changes of mucous type to enable these animals to adapt to the environment, thus altering the concentration of sodium fluoride.O flúor é muito utilizado pelo homem e facilmente encontrado na natureza. Por isso, vários estudos foram realizados visando a toxicidade e o seu efeito cumulativo nos tecidos animais. É descrito que o fluoreto de sódio pode ser empregado no controle do hospedeiro da esquistossomose (Biomphalaria glabrata). Visando a preservação do meio aquático, propõe-se a verificação do efeito do fluoreto de sódio em peixes (Poecilia vivipara). Dez peixes foram submetidos à água declorinada (GC) e o restante foi submetido à água contendo fluoreto de sódio na concentração de 5 ppm (GE), por vinte e um dias. Após esse período, os animais foram sacrificados e suas brânquias foram retiradas. As brânquias foram fixadas, pós-fixadas e desidratadas de acordo com o protocolo das técnicas de histoquímica e análise ultra-estrutural, respectivamente. As peças foram diafanizadas em xilol. Para observação histológica das células mucosas e células do cloro, foi utilizada a técnica de coloração de Hematoxilina + eosina. E para a detecção histoquímica de glicoconjugados utilizou-se as técnicas de P.A.S., P.A.S + amilase salivar, P.A.S. + acetilação, P.A.S. + acetilação reversível, alcian Blue (AB) pH 2,5 + metilação, AB pH 2,5 + metilação reversível, AB pH 2,5 e AB pH 0,5. Observou-se aumento na secreção de muco e na quantidade de células mucosas do epitélio branquial, além de diferenças na reatividade histoquímica das células mucosas do GE comparados ao GC, sugerindo uma resposta adaptativa desses animais ao novo ambiente.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2004-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/628810.1590/S1413-95962004000400009Brazilian Journal of Veterinary Research and Animal Science; Vol. 41 Núm. 4 (2004); 274-280 Brazilian Journal of Veterinary Research and Animal Science; Vol. 41 No. 4 (2004); 274-280 Brazilian Journal of Veterinary Research and Animal Science; v. 41 n. 4 (2004); 274-280 Brazilian Journal of Veterinary Research and Animal Science; V. 41 N. 4 (2004); 274-280 1678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/6288/7819Breseghelo, LeandroCardoso, Márcia PereiraBorges-de-Oliveira, RodinelliCosta, Marcelo Ferreira daBarreto, José Clecildo BezerraSabóia-Morais, Simone Maria Teixiera deYamada, Áureo Tatsumiinfo:eu-repo/semantics/openAccess2020-06-23T04:29:15Zoai:revistas.usp.br:article/6288Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:35.732107Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Efeitos do fluoreto de sódio no epitélio da brânquia do peixe Guaru (Poecilia vivipara) Effects of sodium fluoride in gill epithelium of Guppy fish (Poecilia vivipara) |
title |
Efeitos do fluoreto de sódio no epitélio da brânquia do peixe Guaru (Poecilia vivipara) |
spellingShingle |
Efeitos do fluoreto de sódio no epitélio da brânquia do peixe Guaru (Poecilia vivipara) Breseghelo, Leandro Brânquias Células do cloro Células mucosas Esquistossomose Fluoreto de sódio Chloride cells Fluorine Gills Mucous cells Schistosomiasis |
title_short |
Efeitos do fluoreto de sódio no epitélio da brânquia do peixe Guaru (Poecilia vivipara) |
title_full |
Efeitos do fluoreto de sódio no epitélio da brânquia do peixe Guaru (Poecilia vivipara) |
title_fullStr |
Efeitos do fluoreto de sódio no epitélio da brânquia do peixe Guaru (Poecilia vivipara) |
title_full_unstemmed |
Efeitos do fluoreto de sódio no epitélio da brânquia do peixe Guaru (Poecilia vivipara) |
title_sort |
Efeitos do fluoreto de sódio no epitélio da brânquia do peixe Guaru (Poecilia vivipara) |
author |
Breseghelo, Leandro |
author_facet |
Breseghelo, Leandro Cardoso, Márcia Pereira Borges-de-Oliveira, Rodinelli Costa, Marcelo Ferreira da Barreto, José Clecildo Bezerra Sabóia-Morais, Simone Maria Teixiera de Yamada, Áureo Tatsumi |
author_role |
author |
author2 |
Cardoso, Márcia Pereira Borges-de-Oliveira, Rodinelli Costa, Marcelo Ferreira da Barreto, José Clecildo Bezerra Sabóia-Morais, Simone Maria Teixiera de Yamada, Áureo Tatsumi |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Breseghelo, Leandro Cardoso, Márcia Pereira Borges-de-Oliveira, Rodinelli Costa, Marcelo Ferreira da Barreto, José Clecildo Bezerra Sabóia-Morais, Simone Maria Teixiera de Yamada, Áureo Tatsumi |
dc.subject.por.fl_str_mv |
Brânquias Células do cloro Células mucosas Esquistossomose Fluoreto de sódio Chloride cells Fluorine Gills Mucous cells Schistosomiasis |
topic |
Brânquias Células do cloro Células mucosas Esquistossomose Fluoreto de sódio Chloride cells Fluorine Gills Mucous cells Schistosomiasis |
description |
Fluorine is an element much used by man and easily found in nature. Some studies have been carried out on the toxicity of fluorine and its cumulative effect on animal tissues. Sodium fluoride is described as being used in controlling the host of schistosomiasis (Biomphalaria glabrata). With a view to preserve the aquatic environment, it is proposed to verify the effect of sodium fluoride on fish (Poecilia vivipara). Ten fishes were divided into two groups. The first group was exposed to freshwater with sodium fluoride with a concentration of 5 ppm for a period of 21 days; the second group was exposed to freshwater. After 21 days the animals were sacrificed and their gills removed and processed according to histochemical/histologic methods and ultrastructural study. The gill leaflets were diaphanized in xylol. Histologic analyses using hematoxyline-eosin identified branchial epithelium and revealed mucous and chloride cells. The histochemical methods to detect glycoconjugate contents of mucous cells used: P.A.S, P.A.S. + salivar amylase; P.A.S. + acetylation ; P.A.S. + reversible acetylation; Alcian blue pH 2.5; Alcian blue pH 0.5; Alcian blue pH 2.5 + methylation; and Alcian blue pH 2.5 + reversible methylation. An increase in secretion of mucus and an alteration to the content of the granules were also observed, suggesting behavior changes of mucous type to enable these animals to adapt to the environment, thus altering the concentration of sodium fluoride. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/6288 10.1590/S1413-95962004000400009 |
url |
https://www.revistas.usp.br/bjvras/article/view/6288 |
identifier_str_mv |
10.1590/S1413-95962004000400009 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/6288/7819 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 41 Núm. 4 (2004); 274-280 Brazilian Journal of Veterinary Research and Animal Science; Vol. 41 No. 4 (2004); 274-280 Brazilian Journal of Veterinary Research and Animal Science; v. 41 n. 4 (2004); 274-280 Brazilian Journal of Veterinary Research and Animal Science; V. 41 N. 4 (2004); 274-280 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051556372152320 |