Evaluation of oocyte maturation conditions and bull effect on the in vitro bovine embryo production

Detalhes bibliográficos
Autor(a) principal: Coelho, Lia de Alencar
Data de Publicação: 1998
Outros Autores: Esper, Cesar Roberto, Garcia, Joaquim Mansano, Vantini, Roberta, Silva Filho, Izaltino Rocha, Almeida Jr., Ivo Luis
Tipo de documento: Artigo
Idioma: por
Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
Texto Completo: https://www.revistas.usp.br/bjvras/article/view/5706
Resumo: The conditions of in vitro maturation of bovine oocytes (Exp. I) and bull effect (Exp. II) on cleavage (CR) and the in vitro morulae and blastocysts (MO/BL) rates were evaluated. Imatured oocytes were matured in vitro under different conditions: B-199 medium with 5% estrous cow serum (ECS), 5% fetal calf serum (FCS) and granulosa cells (T1/Exp. I) or B-199 medium with 10% FCS, 14 IU/ml PMSG and 7 IU/ml hCG (T2/Exp I). After 24 h culture, the mature oocytes were inseminated with frozen-thawed semen from two bulls (R1 and R2/Exp. II). In the Exp. I, the T1 and T2 oocytes were inseminated with semen from R1/Exp. II and in the exp. II the semen from both bulls inseminated T1/Exp. I oocytes. After 48 h, the 2-4 cells embryos were transferred into embryo culture droplets and were further cultured for 9 days. The CR of T2/Exp I was higher (p<0.01) than T1/Exp. I. There was a significant difference (p<0.05) between bulls on CR and Mo/Bl production. The use of hormones in a maturation medium has no effect on embryo production, however the procedure seems to be simpler than with granulosa cells. The use of semen from different bulls for in vitro fertilization has affected the subsequent in vitro production of embryos.
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spelling Evaluation of oocyte maturation conditions and bull effect on the in vitro bovine embryo productionAvaliação das condições de maturação oocitária e do efeito do reprodutor na produção in vitro de embriões bovinosMaturaçãoFertilizaçãoIn vitroOócitosBovinosMaturationFertilizationIn vitroOocytesBovineThe conditions of in vitro maturation of bovine oocytes (Exp. I) and bull effect (Exp. II) on cleavage (CR) and the in vitro morulae and blastocysts (MO/BL) rates were evaluated. Imatured oocytes were matured in vitro under different conditions: B-199 medium with 5% estrous cow serum (ECS), 5% fetal calf serum (FCS) and granulosa cells (T1/Exp. I) or B-199 medium with 10% FCS, 14 IU/ml PMSG and 7 IU/ml hCG (T2/Exp I). After 24 h culture, the mature oocytes were inseminated with frozen-thawed semen from two bulls (R1 and R2/Exp. II). In the Exp. I, the T1 and T2 oocytes were inseminated with semen from R1/Exp. II and in the exp. II the semen from both bulls inseminated T1/Exp. I oocytes. After 48 h, the 2-4 cells embryos were transferred into embryo culture droplets and were further cultured for 9 days. The CR of T2/Exp I was higher (p<0.01) than T1/Exp. I. There was a significant difference (p<0.05) between bulls on CR and Mo/Bl production. The use of hormones in a maturation medium has no effect on embryo production, however the procedure seems to be simpler than with granulosa cells. The use of semen from different bulls for in vitro fertilization has affected the subsequent in vitro production of embryos.Avaliaram-se as condições de cultura para maturação dos oócitos (Exp. I) e o efeito do reprodutor (Exp. II) sobre as taxas de clivagem (TC) e de mórulas e blastocistos (MO/BL) produzidos in vitro. Oócitos imaturos foram submetidos a maturação in vitro sob diferentes condições: meio B-199, acrescido de 5% de soro de vaca em estro (SVE), 5% de soro fetal bovino (SFB) e células da granulosa (T1/Exp. I) ou meio B-199, acrescido de 10% de SFB, 14 UI/ml de PMSG e 7 UI/ml de hCG (T2/Exp I). Após 24 h de cultura, os oócitos maturos foram inseminados com sêmen descongelado de dois reprodutores (R1 e R2 /Exp. II) e, após 48 h de incubação, os embriões de 2 e 4 células foram transferidos para placas de cultivo in vitro, onde permaneceram em cultura por mais 9 dias. No Exp. I, os oócitos de T1 e T2 foram inseminados com sêmen do R1/Exp. II. No Exp. II, os oócitos do T1/Exp. I foram inseminados com sêmen de R1 e R2/Exp. II. No Exp. I, a TC foi superior (p<0,01) para T2, quando comparado com T1. A produção de MO/BL não foi diferente (p>;0,05) entre tratamentos. No Exp. II, a TC e a taxa de MO/BL diferiram (p<0,05) para cada reprodutor. A adição de hormônios no meio de maturação, embora não tenha interferido na produção de embriões, parece ser um procedimento mais prático que o uso de células da granulosa. A utilização de sêmen de diferentes reprodutores afetou o subseqüente desenvolvimento embrionário in vitro.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia1998-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/570610.1590/S1413-95961998000300005Brazilian Journal of Veterinary Research and Animal Science; Vol. 35 Núm. 3 (1998); 120-122Brazilian Journal of Veterinary Research and Animal Science; Vol. 35 No. 3 (1998); 120-122Brazilian Journal of Veterinary Research and Animal Science; v. 35 n. 3 (1998); 120-122Brazilian Journal of Veterinary Research and Animal Science; V. 35 N. 3 (1998); 120-1221678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/5706/7237Coelho, Lia de AlencarEsper, Cesar RobertoGarcia, Joaquim MansanoVantini, RobertaSilva Filho, Izaltino RochaAlmeida Jr., Ivo Luisinfo:eu-repo/semantics/openAccess2020-06-23T04:45:21Zoai:revistas.usp.br:article/5706Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:18.342995Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv Evaluation of oocyte maturation conditions and bull effect on the in vitro bovine embryo production
Avaliação das condições de maturação oocitária e do efeito do reprodutor na produção in vitro de embriões bovinos
title Evaluation of oocyte maturation conditions and bull effect on the in vitro bovine embryo production
spellingShingle Evaluation of oocyte maturation conditions and bull effect on the in vitro bovine embryo production
Coelho, Lia de Alencar
Maturação
Fertilização
In vitro
Oócitos
Bovinos
Maturation
Fertilization
In vitro
Oocytes
Bovine
title_short Evaluation of oocyte maturation conditions and bull effect on the in vitro bovine embryo production
title_full Evaluation of oocyte maturation conditions and bull effect on the in vitro bovine embryo production
title_fullStr Evaluation of oocyte maturation conditions and bull effect on the in vitro bovine embryo production
title_full_unstemmed Evaluation of oocyte maturation conditions and bull effect on the in vitro bovine embryo production
title_sort Evaluation of oocyte maturation conditions and bull effect on the in vitro bovine embryo production
author Coelho, Lia de Alencar
author_facet Coelho, Lia de Alencar
Esper, Cesar Roberto
Garcia, Joaquim Mansano
Vantini, Roberta
Silva Filho, Izaltino Rocha
Almeida Jr., Ivo Luis
author_role author
author2 Esper, Cesar Roberto
Garcia, Joaquim Mansano
Vantini, Roberta
Silva Filho, Izaltino Rocha
Almeida Jr., Ivo Luis
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Coelho, Lia de Alencar
Esper, Cesar Roberto
Garcia, Joaquim Mansano
Vantini, Roberta
Silva Filho, Izaltino Rocha
Almeida Jr., Ivo Luis
dc.subject.por.fl_str_mv Maturação
Fertilização
In vitro
Oócitos
Bovinos
Maturation
Fertilization
In vitro
Oocytes
Bovine
topic Maturação
Fertilização
In vitro
Oócitos
Bovinos
Maturation
Fertilization
In vitro
Oocytes
Bovine
description The conditions of in vitro maturation of bovine oocytes (Exp. I) and bull effect (Exp. II) on cleavage (CR) and the in vitro morulae and blastocysts (MO/BL) rates were evaluated. Imatured oocytes were matured in vitro under different conditions: B-199 medium with 5% estrous cow serum (ECS), 5% fetal calf serum (FCS) and granulosa cells (T1/Exp. I) or B-199 medium with 10% FCS, 14 IU/ml PMSG and 7 IU/ml hCG (T2/Exp I). After 24 h culture, the mature oocytes were inseminated with frozen-thawed semen from two bulls (R1 and R2/Exp. II). In the Exp. I, the T1 and T2 oocytes were inseminated with semen from R1/Exp. II and in the exp. II the semen from both bulls inseminated T1/Exp. I oocytes. After 48 h, the 2-4 cells embryos were transferred into embryo culture droplets and were further cultured for 9 days. The CR of T2/Exp I was higher (p<0.01) than T1/Exp. I. There was a significant difference (p<0.05) between bulls on CR and Mo/Bl production. The use of hormones in a maturation medium has no effect on embryo production, however the procedure seems to be simpler than with granulosa cells. The use of semen from different bulls for in vitro fertilization has affected the subsequent in vitro production of embryos.
publishDate 1998
dc.date.none.fl_str_mv 1998-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/5706
10.1590/S1413-95961998000300005
url https://www.revistas.usp.br/bjvras/article/view/5706
identifier_str_mv 10.1590/S1413-95961998000300005
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/5706/7237
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 35 Núm. 3 (1998); 120-122
Brazilian Journal of Veterinary Research and Animal Science; Vol. 35 No. 3 (1998); 120-122
Brazilian Journal of Veterinary Research and Animal Science; v. 35 n. 3 (1998); 120-122
Brazilian Journal of Veterinary Research and Animal Science; V. 35 N. 3 (1998); 120-122
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
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