Efficiency of salivary progesterone measurement to identify the ovulation in bitches
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/51707 |
Resumo: | Several authors have emphasized the importance of monitoring estrous cycle in bitches and mentioned technique examples of how it can be done. The aim of this study was to evaluate the salivary progesterone quantification technique in order to identify ovulation in this species. To compound the experimental group, 13 bitches of different breeds (no specific breed, English Bulldog, Bernesse Mountain Dog)and different ages (from 11 months to 9 years old) were used. Blood and saliva samples were collected simultaneously in all animals, starting about the first day of proestrus clinical signs. Salivary samples were collected with a specific commercial device (Salivette®). This method was effective, since it has become possible to obtain enough volume in almost all samples to quantify salivary progesterone (100 μLfor duplicate quantification). Serum progesterone was quantified by radioimmunoassay and salivary progesterone by enzyme immunoassay, both of them with commercial kits. There was an increasing, linear and positive correlation between salivary and serum progesterone (r=0.704; p<0.0001) in bitches. One of the animals had an anovulatory cycle, in which, for 13 days, seric progesterone levels were maintained between 1.67 and 3.76 ng/mL and salivary progesterone levels were maintained between 19.55 and 236.77 pg/mL. It was concluded that salivary progesterone levels measured by enzyme immunoassay is a technique that can be used to evaluate the presence or absence of ovulation in bitches. |
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Brazilian Journal of Veterinary Research and Animal Science |
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Efficiency of salivary progesterone measurement to identify the ovulation in bitchesEficiência da mensuração de progesterona salivar para identificar a ovulação em cadelasImunoensaioProgesteronaSalivaCadelasReproduçãoImmunoassayProgesteroneSalivaBitchesReproductionSeveral authors have emphasized the importance of monitoring estrous cycle in bitches and mentioned technique examples of how it can be done. The aim of this study was to evaluate the salivary progesterone quantification technique in order to identify ovulation in this species. To compound the experimental group, 13 bitches of different breeds (no specific breed, English Bulldog, Bernesse Mountain Dog)and different ages (from 11 months to 9 years old) were used. Blood and saliva samples were collected simultaneously in all animals, starting about the first day of proestrus clinical signs. Salivary samples were collected with a specific commercial device (Salivette®). This method was effective, since it has become possible to obtain enough volume in almost all samples to quantify salivary progesterone (100 μLfor duplicate quantification). Serum progesterone was quantified by radioimmunoassay and salivary progesterone by enzyme immunoassay, both of them with commercial kits. There was an increasing, linear and positive correlation between salivary and serum progesterone (r=0.704; p<0.0001) in bitches. One of the animals had an anovulatory cycle, in which, for 13 days, seric progesterone levels were maintained between 1.67 and 3.76 ng/mL and salivary progesterone levels were maintained between 19.55 and 236.77 pg/mL. It was concluded that salivary progesterone levels measured by enzyme immunoassay is a technique that can be used to evaluate the presence or absence of ovulation in bitches.Vários autores enfatizaram a importância do monitoramento do ciclo estral em cadelas e citaram exemplos de como realizar tal procedimento. O objetivo deste estudo foi avaliar a técnica de dosagem de progesterona salivar em cadelas para identificar a ovulação nesta espécie. Para composição do grupo experimental, foram utilizadas 13 cadelas, de diferentes raças (sem raça definida, Buldogue Inglês, Bernesse Mountain Dog) e diferentes idades (11 meses a 9 anos).Amostras de sangue e saliva foram colhidas simultaneamente em todos os animais, a partir dos primeiros sinais clínicos de proestro. Amostras salivares foram obtidas com o uso de dispositivo comercial específico (Salivette®). Este método foi eficaz, visto ter tornado possível obter volume suficiente para dosagem de progesterona na grande maioria das amostras(100 μL para dosagens em duplicata). As concentrações de progesterona no soro foram determinadas pela técnica de radioimunoensaio e na saliva por enzimaimunoensaio, ambas com kits comerciais. Observou-se uma relação linear crescente e positiva entre a progesterona sérica e salivar (r=0,704; p<0,0001) em cadelas. Uma das cadelas apresentou ciclo anovulatório, no qual, durante 13 dias, as concentrações séricas de progesterona sérica se mantiveram entre 1,67 e3,76 ng/mL e as concentrações salivares de progesterona entre 19,55 e 236,77 pg/mL. Conclui-se que as concentrações de progesterona salivar mensuradas pela técnica de enzimaimunoensaio podem ser utilizadas para avaliar a ocorrência ou ausência de ovulação em cadelas.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2012-08-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/5170710.11606/issn.1678-4456.v49i4p269-276Brazilian Journal of Veterinary Research and Animal Science; Vol. 49 Núm. 4 (2012); 269-276Brazilian Journal of Veterinary Research and Animal Science; Vol. 49 No. 4 (2012); 269-276Brazilian Journal of Veterinary Research and Animal Science; v. 49 n. 4 (2012); 269-276Brazilian Journal of Veterinary Research and Animal Science; V. 49 N. 4 (2012); 269-2761678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/51707/55768Lopes, Patricia RottaFurtado, Priscila ViauOliveira, Claudio Alvarenga deinfo:eu-repo/semantics/openAccess2020-06-23T04:08:13Zoai:revistas.usp.br:article/51707Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:20.283595Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Efficiency of salivary progesterone measurement to identify the ovulation in bitches Eficiência da mensuração de progesterona salivar para identificar a ovulação em cadelas |
title |
Efficiency of salivary progesterone measurement to identify the ovulation in bitches |
spellingShingle |
Efficiency of salivary progesterone measurement to identify the ovulation in bitches Lopes, Patricia Rotta Imunoensaio Progesterona Saliva Cadelas Reprodução Immunoassay Progesterone Saliva Bitches Reproduction |
title_short |
Efficiency of salivary progesterone measurement to identify the ovulation in bitches |
title_full |
Efficiency of salivary progesterone measurement to identify the ovulation in bitches |
title_fullStr |
Efficiency of salivary progesterone measurement to identify the ovulation in bitches |
title_full_unstemmed |
Efficiency of salivary progesterone measurement to identify the ovulation in bitches |
title_sort |
Efficiency of salivary progesterone measurement to identify the ovulation in bitches |
author |
Lopes, Patricia Rotta |
author_facet |
Lopes, Patricia Rotta Furtado, Priscila Viau Oliveira, Claudio Alvarenga de |
author_role |
author |
author2 |
Furtado, Priscila Viau Oliveira, Claudio Alvarenga de |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Lopes, Patricia Rotta Furtado, Priscila Viau Oliveira, Claudio Alvarenga de |
dc.subject.por.fl_str_mv |
Imunoensaio Progesterona Saliva Cadelas Reprodução Immunoassay Progesterone Saliva Bitches Reproduction |
topic |
Imunoensaio Progesterona Saliva Cadelas Reprodução Immunoassay Progesterone Saliva Bitches Reproduction |
description |
Several authors have emphasized the importance of monitoring estrous cycle in bitches and mentioned technique examples of how it can be done. The aim of this study was to evaluate the salivary progesterone quantification technique in order to identify ovulation in this species. To compound the experimental group, 13 bitches of different breeds (no specific breed, English Bulldog, Bernesse Mountain Dog)and different ages (from 11 months to 9 years old) were used. Blood and saliva samples were collected simultaneously in all animals, starting about the first day of proestrus clinical signs. Salivary samples were collected with a specific commercial device (Salivette®). This method was effective, since it has become possible to obtain enough volume in almost all samples to quantify salivary progesterone (100 μLfor duplicate quantification). Serum progesterone was quantified by radioimmunoassay and salivary progesterone by enzyme immunoassay, both of them with commercial kits. There was an increasing, linear and positive correlation between salivary and serum progesterone (r=0.704; p<0.0001) in bitches. One of the animals had an anovulatory cycle, in which, for 13 days, seric progesterone levels were maintained between 1.67 and 3.76 ng/mL and salivary progesterone levels were maintained between 19.55 and 236.77 pg/mL. It was concluded that salivary progesterone levels measured by enzyme immunoassay is a technique that can be used to evaluate the presence or absence of ovulation in bitches. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-08-22 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/51707 10.11606/issn.1678-4456.v49i4p269-276 |
url |
https://www.revistas.usp.br/bjvras/article/view/51707 |
identifier_str_mv |
10.11606/issn.1678-4456.v49i4p269-276 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/51707/55768 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 49 Núm. 4 (2012); 269-276 Brazilian Journal of Veterinary Research and Animal Science; Vol. 49 No. 4 (2012); 269-276 Brazilian Journal of Veterinary Research and Animal Science; v. 49 n. 4 (2012); 269-276 Brazilian Journal of Veterinary Research and Animal Science; V. 49 N. 4 (2012); 269-276 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051563154341888 |