Cryopreservation of mouse morulae in glycerol, sucrose and honeybee royal jelly
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/5804 |
Resumo: | Compacted mouse morulae were frozen at 0.3ºC/min. or 0.5ºC/min. from -6ºC to -24ºC or -32ºC in 10% of glycerol plus different sucrose concentrations with or without 0.1% of honeybee royal jelly. Embryos were thawed in water bath at 22ºC for 20 seconds and cryoprotectant dilution was done in three steps. Embryos were cultured in Whittens medium for 24, 48 and 72 hours at 37ºC, 5% of CO2 and 100% of humidity. The in vitro development ranged from 56.6% to 100% after 72 hours. Expanded blastocysts were transferred to pseudopregnant recipients on the third day of the estrous cycle. Viable fetuses rates for embryos frozen to -24 or -32ºC at 0.3ºC/minute in 10% glycerol + 10% sucrose, 10% glycerol + 10% sucrose + 0.1% honeybee royal jelly, 10% glycerol + 0.1% honeybee royal jelly or 10% glycerol were respectively: 28.1% and 13.6%, 48.7% and 31.9%, 28.6% and 13.2%, 20.0% and 42.4%. Viable fetuses for embryos frozen to -24ºC or -32ºC at 0.5ºC/minute in 10% glycerol + 10% sucrose or 10% glycerol + 10% sucrose + 0.1% honeybee royal jelly were respectively 29.0% and 15.3%, 48.8% and 32.0%. We can conclude that addition of 10% sucrose to 10% glycerol was efficient for embryo freezing at 0.3 or 0.5ºC/minute and plunged in liquid nitrogen at -24ºC. The honeybee royal jelly addition provided higher viable fetuses rates when embryos were cooled at 0.3 or 0.5ºC/minute and plunged in liquid nitrogen at -24ºC. |
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Brazilian Journal of Veterinary Research and Animal Science |
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Cryopreservation of mouse morulae in glycerol, sucrose and honeybee royal jellyCriopreservação de mórulas de camundongos em glicerol, sacarose e geléia realCamundongosEmbriõesCriopreservaçãoSacaroseGeléia realMiceEmbryoCryopreservationSucroseRoyal jellyCompacted mouse morulae were frozen at 0.3ºC/min. or 0.5ºC/min. from -6ºC to -24ºC or -32ºC in 10% of glycerol plus different sucrose concentrations with or without 0.1% of honeybee royal jelly. Embryos were thawed in water bath at 22ºC for 20 seconds and cryoprotectant dilution was done in three steps. Embryos were cultured in Whittens medium for 24, 48 and 72 hours at 37ºC, 5% of CO2 and 100% of humidity. The in vitro development ranged from 56.6% to 100% after 72 hours. Expanded blastocysts were transferred to pseudopregnant recipients on the third day of the estrous cycle. Viable fetuses rates for embryos frozen to -24 or -32ºC at 0.3ºC/minute in 10% glycerol + 10% sucrose, 10% glycerol + 10% sucrose + 0.1% honeybee royal jelly, 10% glycerol + 0.1% honeybee royal jelly or 10% glycerol were respectively: 28.1% and 13.6%, 48.7% and 31.9%, 28.6% and 13.2%, 20.0% and 42.4%. Viable fetuses for embryos frozen to -24ºC or -32ºC at 0.5ºC/minute in 10% glycerol + 10% sucrose or 10% glycerol + 10% sucrose + 0.1% honeybee royal jelly were respectively 29.0% and 15.3%, 48.8% and 32.0%. We can conclude that addition of 10% sucrose to 10% glycerol was efficient for embryo freezing at 0.3 or 0.5ºC/minute and plunged in liquid nitrogen at -24ºC. The honeybee royal jelly addition provided higher viable fetuses rates when embryos were cooled at 0.3 or 0.5ºC/minute and plunged in liquid nitrogen at -24ºC.Embriões de camundongos foram congelados em 10% de glicerol adicionado de várias concentrações de sacarose e/ou 0,1% de geléia real, nas velocidades de 0,3ºC ou 0,5ºC/minuto até -24ºC ou -32ºC e descongelados em água a 22ºC durante 20 segundos. A diluição dos crioprotetores foi realizada em três etapas e o cultivo dos embriões no meio de Whitten em estufa com 5% de CO2, 100% de umidade e 37ºC por 72 horas. O desenvolvimento in vitro até blastocisto variou entre 56,6% e 93,4%, mostrando que 10% de sacarose + 10% de glicerol foi eficiente na congelação. Blastocistos expandidos oriundos de embriões congelados até -24 ou -32ºC à velocidade de 0,3ºC/minuto em soluções contendo 10% de glicerol + 10% de sacarose; 10% de glicerol + 10% de sacarose + 0,1% de geléia real; 10% de glicerol + 0,1% de geléia real ou 10% de glicerol, transferidos para receptoras pseudoprenhes, apresentaram, respectivamente, taxas de fetos viáveis de 28,1% e 13,6%, 48,7% e 31,9%, 28,6% e 13,2%, 20,0% e 42,4%. Embriões congelados até -24ºC ou -32ºC a 0,5ºC/minuto nas soluções de 10% de glicerol + 10% de sacarose ou 10% de glicerol + 10% de sacarose + 0,1% de geléia real apresentaram, respectivamente, taxas de fetos viáveis de 29,0% e 15,3%, 48,8% e 32,0%. Adição de sacarose e de geléia real ao meio de congelação contendo 10% de glicerol proporcionou maiores taxas de fetos viáveis a 0,3ºC e 0,5ºC/minuto e imersão em nitrogênio líquido a -24ºC.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2000-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/580410.1590/S1413-95962000000400009Brazilian Journal of Veterinary Research and Animal Science; Vol. 37 Núm. 4 (2000); 307-311Brazilian Journal of Veterinary Research and Animal Science; Vol. 37 No. 4 (2000); 307-311Brazilian Journal of Veterinary Research and Animal Science; v. 37 n. 4 (2000); 307-311Brazilian Journal of Veterinary Research and Animal Science; V. 37 N. 4 (2000); 307-3111678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/5804/62491Visintin, José AntônioGarcia, José FernandoPantano, ThaisD'Avila Assumpção, Mayra Elena Ortizinfo:eu-repo/semantics/openAccess2020-06-23T04:42:06Zoai:revistas.usp.br:article/5804Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:23.247010Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Cryopreservation of mouse morulae in glycerol, sucrose and honeybee royal jelly Criopreservação de mórulas de camundongos em glicerol, sacarose e geléia real |
title |
Cryopreservation of mouse morulae in glycerol, sucrose and honeybee royal jelly |
spellingShingle |
Cryopreservation of mouse morulae in glycerol, sucrose and honeybee royal jelly Visintin, José Antônio Camundongos Embriões Criopreservação Sacarose Geléia real Mice Embryo Cryopreservation Sucrose Royal jelly |
title_short |
Cryopreservation of mouse morulae in glycerol, sucrose and honeybee royal jelly |
title_full |
Cryopreservation of mouse morulae in glycerol, sucrose and honeybee royal jelly |
title_fullStr |
Cryopreservation of mouse morulae in glycerol, sucrose and honeybee royal jelly |
title_full_unstemmed |
Cryopreservation of mouse morulae in glycerol, sucrose and honeybee royal jelly |
title_sort |
Cryopreservation of mouse morulae in glycerol, sucrose and honeybee royal jelly |
author |
Visintin, José Antônio |
author_facet |
Visintin, José Antônio Garcia, José Fernando Pantano, Thais D'Avila Assumpção, Mayra Elena Ortiz |
author_role |
author |
author2 |
Garcia, José Fernando Pantano, Thais D'Avila Assumpção, Mayra Elena Ortiz |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Visintin, José Antônio Garcia, José Fernando Pantano, Thais D'Avila Assumpção, Mayra Elena Ortiz |
dc.subject.por.fl_str_mv |
Camundongos Embriões Criopreservação Sacarose Geléia real Mice Embryo Cryopreservation Sucrose Royal jelly |
topic |
Camundongos Embriões Criopreservação Sacarose Geléia real Mice Embryo Cryopreservation Sucrose Royal jelly |
description |
Compacted mouse morulae were frozen at 0.3ºC/min. or 0.5ºC/min. from -6ºC to -24ºC or -32ºC in 10% of glycerol plus different sucrose concentrations with or without 0.1% of honeybee royal jelly. Embryos were thawed in water bath at 22ºC for 20 seconds and cryoprotectant dilution was done in three steps. Embryos were cultured in Whittens medium for 24, 48 and 72 hours at 37ºC, 5% of CO2 and 100% of humidity. The in vitro development ranged from 56.6% to 100% after 72 hours. Expanded blastocysts were transferred to pseudopregnant recipients on the third day of the estrous cycle. Viable fetuses rates for embryos frozen to -24 or -32ºC at 0.3ºC/minute in 10% glycerol + 10% sucrose, 10% glycerol + 10% sucrose + 0.1% honeybee royal jelly, 10% glycerol + 0.1% honeybee royal jelly or 10% glycerol were respectively: 28.1% and 13.6%, 48.7% and 31.9%, 28.6% and 13.2%, 20.0% and 42.4%. Viable fetuses for embryos frozen to -24ºC or -32ºC at 0.5ºC/minute in 10% glycerol + 10% sucrose or 10% glycerol + 10% sucrose + 0.1% honeybee royal jelly were respectively 29.0% and 15.3%, 48.8% and 32.0%. We can conclude that addition of 10% sucrose to 10% glycerol was efficient for embryo freezing at 0.3 or 0.5ºC/minute and plunged in liquid nitrogen at -24ºC. The honeybee royal jelly addition provided higher viable fetuses rates when embryos were cooled at 0.3 or 0.5ºC/minute and plunged in liquid nitrogen at -24ºC. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/5804 10.1590/S1413-95962000000400009 |
url |
https://www.revistas.usp.br/bjvras/article/view/5804 |
identifier_str_mv |
10.1590/S1413-95962000000400009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/5804/62491 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 37 Núm. 4 (2000); 307-311 Brazilian Journal of Veterinary Research and Animal Science; Vol. 37 No. 4 (2000); 307-311 Brazilian Journal of Veterinary Research and Animal Science; v. 37 n. 4 (2000); 307-311 Brazilian Journal of Veterinary Research and Animal Science; V. 37 N. 4 (2000); 307-311 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051555156852736 |