Effects of dietary nitrogen level on semen characteristics of sheep
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
DOI: | 10.1590/S1413-95962000000500008 |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/5789 |
Resumo: | The research looked upon the influence of dietary nitrogen level or equivalent ruminal degradable protein (RDP) on semen biochemistry characteristics and freezability of sheep spermatozoa. Eighteen young rams, were allocated in tree blocs of six animals each, according to scrotal circumference. The rams were randomly distributed to three treatments: A (control) a maintenance diet, B and C, maintenance diet added 20 g and 40 g of urea (75% and 150% of RDP), respectively. Three weeks after dietary adaptation, blood and semen samples were collected every week for nine weeks, to analyze blood plasma and semen levels of urea, transaminases (AST and ALT), acid phosphatases, frutose and citric acid. Once a week for six weeks the motility percent, motility rates, abnormal acrosome percent and abnormal total percent between pre-freezing and post-thaw of cryoprotected semen samples were freezing, evaluated and calculated to mathematical difference. There was a linear regression (p < 0.01) between levels of UP 18.26 mg%, 34.42 mg% and 49.77 mg%, and levels of semen urea 32.47 mg%, 51.44 mg% e 60.46 mg%, for treatments A, B and C respectively. There was no effect of treatments on fructose, citric acid or enzymes acid phosphatase and transaminases levels, and of motility percent, motility rating, abnormal acrosome percent and abnormal total percent differences. The excess of nitrogen or RDP dietary levels elevates urea in blood plasma and semen, but did not affect biochemistry characteristics and freezability of rams spermatozoa. |
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Brazilian Journal of Veterinary Research and Animal Science |
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Effects of dietary nitrogen level on semen characteristics of sheepEfeitos do nível de nitrogênio na dieta sobre características do sêmen de ovinosSêmen animalNitrogênioReprodução animalBioquímicaOvinosSemenNitrogenAnimal ReproductionBiochemistrySheepThe research looked upon the influence of dietary nitrogen level or equivalent ruminal degradable protein (RDP) on semen biochemistry characteristics and freezability of sheep spermatozoa. Eighteen young rams, were allocated in tree blocs of six animals each, according to scrotal circumference. The rams were randomly distributed to three treatments: A (control) a maintenance diet, B and C, maintenance diet added 20 g and 40 g of urea (75% and 150% of RDP), respectively. Three weeks after dietary adaptation, blood and semen samples were collected every week for nine weeks, to analyze blood plasma and semen levels of urea, transaminases (AST and ALT), acid phosphatases, frutose and citric acid. Once a week for six weeks the motility percent, motility rates, abnormal acrosome percent and abnormal total percent between pre-freezing and post-thaw of cryoprotected semen samples were freezing, evaluated and calculated to mathematical difference. There was a linear regression (p < 0.01) between levels of UP 18.26 mg%, 34.42 mg% and 49.77 mg%, and levels of semen urea 32.47 mg%, 51.44 mg% e 60.46 mg%, for treatments A, B and C respectively. There was no effect of treatments on fructose, citric acid or enzymes acid phosphatase and transaminases levels, and of motility percent, motility rating, abnormal acrosome percent and abnormal total percent differences. The excess of nitrogen or RDP dietary levels elevates urea in blood plasma and semen, but did not affect biochemistry characteristics and freezability of rams spermatozoa.No presente experimento foi pesquisada a influência do nível de nitrogênio ou de equivalente em proteína degradável no rúmen (PDR) da ração sobre características bioquímicas do sêmen e da congelação dos espermatozóides de carneiros deslanados. Empregaram-se 18 carneiros jovens divididos em três blocos de seis animais cada, conforme seus perímetros escrotais, e distribuídos ao acaso para três tratamentos: A (controle), alimentados com ração de manutenção, B e C alimentados com ração de manutenção e acréscimo de 20 g e 40 g de uréia (75% de PDR e 150% de PDR), respectivamente. Após três semanas de adaptação, coletaram-se amostras de sangue e sêmen (vagina artificial) de cada animal, semanalmente durante nove semanas, e determinados os níveis de uréia no plasma sangüíneo (UP) e de uréia, transaminases (AST, ALT), fosfatase ácida, frutose e ácido cítrico no sêmen. Durante seis semanas, foram determinadas as diferenças de motilidade, vigor, patologia de acrossoma e patologia total entre a pré e a pós-congelação de amostras de sêmen crioprotegido. Houve regressão linear (p < 0,01) para os níveis de UP, 18,26 mg%, 34,42 mg% e 49,77 mg%, e para os níveis de uréia no sêmen 32,47 mg%, 51,44 mg% e 60,46 mg%, respectivamente para os tratamentos A, B e C. Não houve efeito dos tratamentos sobre concentrações seminais de frutose, ácido cítrico ou das enzimas fosfatase ácida e transaminases nem sobre diferenças na motilidade, vigor, patologia de acrossoma e patologia total dos espermatozóides. Os teores elevados de nitrogênio ou PDR na ração de carneiros deslanados elevaram os níveis de uréia no plasma sangüíneo e sêmen, mas não afetaram características bioquímicas do sêmen ou de congelabilidade dos espermatozóides.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2000-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/578910.1590/S1413-95962000000500008Brazilian Journal of Veterinary Research and Animal Science; Vol. 37 Núm. 5 (2000); 388-394Brazilian Journal of Veterinary Research and Animal Science; Vol. 37 No. 5 (2000); 388-394Brazilian Journal of Veterinary Research and Animal Science; v. 37 n. 5 (2000); 388-394Brazilian Journal of Veterinary Research and Animal Science; V. 37 N. 5 (2000); 388-3941678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/5789/62835Gonzales, Rodrigo Alonso ForeroLucci, Carlos de SousaCortada, Carmen Neusa MartinsRodrigues, Paulo Henrique MazzaRodrigues, Renato Ranziniinfo:eu-repo/semantics/openAccess2020-06-23T04:42:35Zoai:revistas.usp.br:article/5789Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:22.508941Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Effects of dietary nitrogen level on semen characteristics of sheep Efeitos do nível de nitrogênio na dieta sobre características do sêmen de ovinos |
title |
Effects of dietary nitrogen level on semen characteristics of sheep |
spellingShingle |
Effects of dietary nitrogen level on semen characteristics of sheep Effects of dietary nitrogen level on semen characteristics of sheep Gonzales, Rodrigo Alonso Forero Sêmen animal Nitrogênio Reprodução animal Bioquímica Ovinos Semen Nitrogen Animal Reproduction Biochemistry Sheep Gonzales, Rodrigo Alonso Forero Sêmen animal Nitrogênio Reprodução animal Bioquímica Ovinos Semen Nitrogen Animal Reproduction Biochemistry Sheep |
title_short |
Effects of dietary nitrogen level on semen characteristics of sheep |
title_full |
Effects of dietary nitrogen level on semen characteristics of sheep |
title_fullStr |
Effects of dietary nitrogen level on semen characteristics of sheep Effects of dietary nitrogen level on semen characteristics of sheep |
title_full_unstemmed |
Effects of dietary nitrogen level on semen characteristics of sheep Effects of dietary nitrogen level on semen characteristics of sheep |
title_sort |
Effects of dietary nitrogen level on semen characteristics of sheep |
author |
Gonzales, Rodrigo Alonso Forero |
author_facet |
Gonzales, Rodrigo Alonso Forero Gonzales, Rodrigo Alonso Forero Lucci, Carlos de Sousa Cortada, Carmen Neusa Martins Rodrigues, Paulo Henrique Mazza Rodrigues, Renato Ranzini Lucci, Carlos de Sousa Cortada, Carmen Neusa Martins Rodrigues, Paulo Henrique Mazza Rodrigues, Renato Ranzini |
author_role |
author |
author2 |
Lucci, Carlos de Sousa Cortada, Carmen Neusa Martins Rodrigues, Paulo Henrique Mazza Rodrigues, Renato Ranzini |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Gonzales, Rodrigo Alonso Forero Lucci, Carlos de Sousa Cortada, Carmen Neusa Martins Rodrigues, Paulo Henrique Mazza Rodrigues, Renato Ranzini |
dc.subject.por.fl_str_mv |
Sêmen animal Nitrogênio Reprodução animal Bioquímica Ovinos Semen Nitrogen Animal Reproduction Biochemistry Sheep |
topic |
Sêmen animal Nitrogênio Reprodução animal Bioquímica Ovinos Semen Nitrogen Animal Reproduction Biochemistry Sheep |
description |
The research looked upon the influence of dietary nitrogen level or equivalent ruminal degradable protein (RDP) on semen biochemistry characteristics and freezability of sheep spermatozoa. Eighteen young rams, were allocated in tree blocs of six animals each, according to scrotal circumference. The rams were randomly distributed to three treatments: A (control) a maintenance diet, B and C, maintenance diet added 20 g and 40 g of urea (75% and 150% of RDP), respectively. Three weeks after dietary adaptation, blood and semen samples were collected every week for nine weeks, to analyze blood plasma and semen levels of urea, transaminases (AST and ALT), acid phosphatases, frutose and citric acid. Once a week for six weeks the motility percent, motility rates, abnormal acrosome percent and abnormal total percent between pre-freezing and post-thaw of cryoprotected semen samples were freezing, evaluated and calculated to mathematical difference. There was a linear regression (p < 0.01) between levels of UP 18.26 mg%, 34.42 mg% and 49.77 mg%, and levels of semen urea 32.47 mg%, 51.44 mg% e 60.46 mg%, for treatments A, B and C respectively. There was no effect of treatments on fructose, citric acid or enzymes acid phosphatase and transaminases levels, and of motility percent, motility rating, abnormal acrosome percent and abnormal total percent differences. The excess of nitrogen or RDP dietary levels elevates urea in blood plasma and semen, but did not affect biochemistry characteristics and freezability of rams spermatozoa. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/5789 10.1590/S1413-95962000000500008 |
url |
https://www.revistas.usp.br/bjvras/article/view/5789 |
identifier_str_mv |
10.1590/S1413-95962000000500008 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/5789/62835 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 37 Núm. 5 (2000); 388-394 Brazilian Journal of Veterinary Research and Animal Science; Vol. 37 No. 5 (2000); 388-394 Brazilian Journal of Veterinary Research and Animal Science; v. 37 n. 5 (2000); 388-394 Brazilian Journal of Veterinary Research and Animal Science; V. 37 N. 5 (2000); 388-394 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1822181027304439809 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S1413-95962000000500008 |