Developmental expression of sexual differences in open-field behaviour and plasma cholinesterase activity in male, female and masculinized female rats

Detalhes bibliográficos
Autor(a) principal: Felicio, Luciano Freitas
Data de Publicação: 1995
Outros Autores: Spinosa, Helenice de Souza, Rocha, Luiz Carlos de Sa, Hucke, Erica Engelberg Teixeira da Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
Texto Completo: https://www.revistas.usp.br/bjvras/article/view/52082
Resumo: Sexual differences in behaviour and metabolism are well recognized. While some of these differences are related to testosterone exposure during neonatal life, others do not depend on the organizational action of androgens during early development. The objective of the following experiments was to study the development of sexual differences in plasma cholinesterase activity and to determine if these differences were related to testosterone exposure postnatally. Open-field activity was also recorded as a behavioral indicator of the actions of testosterone on sexual differentiation of the central nervous system. Three treatment groups of animals were used: normal male, normal female, and masculinized female rats (1 mg testosterone, SC, on day 2 of postnatal life). Open-field behaviour was measured on three consecutive days just after weaning (21 -23 days of age), in association with the onset of puberty (30 - 36 days of age), or as adults (90 - 110 days of age); plasma cholinesterase activity was measured at 22, 30 - 36, and 90 - 110 days of age. As expected a sex difference in open-field activity was found between normal males and females. Postnatal androgen treatment in females decreased open-field activity in adulthood to levels similar to those found in normal males. Similar differences were observed just after weaning, but not at 30-36 days of age. In contrast, significant differences in cholinesterase activity were observed in adult animals, but not at days 22 and 30 - 33 of age. Masculinized female rats showed no differences in plasma cholinesterase activity when compared to normal female rats, both groups differing from males. These data suggest that sexual differences in plasma cholinesterase activity in adult rats, unlike differences in open-field behaviour, are not dependent on testosterone exposure during postnatal life. In addition, the results have shown that under stress (weaning) sex differences in open-field behaviour can be observed as early as at 21 -23 days of age.
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spelling Developmental expression of sexual differences in open-field behaviour and plasma cholinesterase activity in male, female and masculinized female ratsExpressão comportamental das diferenças sexuais no campo aberto e na atividade da colinesterase plasmática de ratos machos, fêmeas e fêmeas masculinizadasCaracterísticas sexuaisColinesteraseTestosteronaComportamento animalSex characteristicCholinesteraseTestosteroneAnimal behaviorSexual differences in behaviour and metabolism are well recognized. While some of these differences are related to testosterone exposure during neonatal life, others do not depend on the organizational action of androgens during early development. The objective of the following experiments was to study the development of sexual differences in plasma cholinesterase activity and to determine if these differences were related to testosterone exposure postnatally. Open-field activity was also recorded as a behavioral indicator of the actions of testosterone on sexual differentiation of the central nervous system. Three treatment groups of animals were used: normal male, normal female, and masculinized female rats (1 mg testosterone, SC, on day 2 of postnatal life). Open-field behaviour was measured on three consecutive days just after weaning (21 -23 days of age), in association with the onset of puberty (30 - 36 days of age), or as adults (90 - 110 days of age); plasma cholinesterase activity was measured at 22, 30 - 36, and 90 - 110 days of age. As expected a sex difference in open-field activity was found between normal males and females. Postnatal androgen treatment in females decreased open-field activity in adulthood to levels similar to those found in normal males. Similar differences were observed just after weaning, but not at 30-36 days of age. In contrast, significant differences in cholinesterase activity were observed in adult animals, but not at days 22 and 30 - 33 of age. Masculinized female rats showed no differences in plasma cholinesterase activity when compared to normal female rats, both groups differing from males. These data suggest that sexual differences in plasma cholinesterase activity in adult rats, unlike differences in open-field behaviour, are not dependent on testosterone exposure during postnatal life. In addition, the results have shown that under stress (weaning) sex differences in open-field behaviour can be observed as early as at 21 -23 days of age.Sabe-se que algumas diferenças sexuais no metabolismo e no comportamento estão relacionadas com o efeito neonatal da testosterona e outras não sofrem esse tipo de influência. O objetivo desses experimentos foi estudar o desenvolvimento das diferenças sexuais na atividade da colinesterase plasmática e de determinar se essas diferenças seriam relacionadas com a exposição pós-natal à testosterona em ratos. A atividade geral no campo aberto foi também verificada como um indicador comportamental das ações da testosterona na diferenciação sexual do sistema nervoso central. Foram usados três grupos de animais: machos normais, fêmeas normais e fêmeas masculinizadas (1 mg testosterona. SC, no 2º. dia de vida pós-natal). A atividade geral no campo aberto foi medida durante três dias consecutivos logo após o desmame (21 - 23 dias de idade), durante o início da puberdade (30 - 36 dias de idade) e nos adultos (90 - 110 dias de idade); a atividade da colinesterase plasmática foi medida aos 22, 30 - 36 e 90 - 110 dias de idade. Como esperado, foi encontrada uma diferença sexual no campo aberto entre machos e fêmeas normais. O tratamento pós-natal com andrógeno nas fêmeas diminuiu a atividade no campo aberto na idade adulta a padrões similares àqueles observados para machos normais. Foram observadas diferenças similares logo após o desmame, mas não aos 22 e aos 30 - 36 dias de idade. Em contraste, foram observadas diferenças significantes na atividade da colinesterase de animais adultos mas não nos dias 22 e 30 - 33 de idade. Quando comparadas às fêmeas normais, as fêmeas masculinizadas não apresentaram diferenças na atividade da colinesterase plasmática, sendo que esses dois grupos foram diferentes dos machos. Esses resultados sugerem que as diferenças sexuais na atividade da colinesterase plasmática de ratos adultos não são dependentes de exposição a testosterona durante o início da vida pós-natal. Além disso os resultados demonstram que sob estresse (desmame) as diferenças sexuais no comportamento no campo aberto podem ser observadas já aos 21-23 dias de idade.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia1995-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/5208210.11606/issn.1678-4456.bjvras.1994.52082Brazilian Journal of Veterinary Research and Animal Science; Vol. 32 Núm. 1 (1995); 5-10Brazilian Journal of Veterinary Research and Animal Science; Vol. 32 No. 1 (1995); 5-10Brazilian Journal of Veterinary Research and Animal Science; v. 32 n. 1 (1995); 5-10Brazilian Journal of Veterinary Research and Animal Science; V. 32 N. 1 (1995); 5-101678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/52082/56132Felicio, Luciano FreitasSpinosa, Helenice de SouzaRocha, Luiz Carlos de SaHucke, Erica Engelberg Teixeira da Silvainfo:eu-repo/semantics/openAccess2020-06-23T04:51:58Zoai:revistas.usp.br:article/52082Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:29.444373Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv Developmental expression of sexual differences in open-field behaviour and plasma cholinesterase activity in male, female and masculinized female rats
Expressão comportamental das diferenças sexuais no campo aberto e na atividade da colinesterase plasmática de ratos machos, fêmeas e fêmeas masculinizadas
title Developmental expression of sexual differences in open-field behaviour and plasma cholinesterase activity in male, female and masculinized female rats
spellingShingle Developmental expression of sexual differences in open-field behaviour and plasma cholinesterase activity in male, female and masculinized female rats
Felicio, Luciano Freitas
Características sexuais
Colinesterase
Testosterona
Comportamento animal
Sex characteristic
Cholinesterase
Testosterone
Animal behavior
title_short Developmental expression of sexual differences in open-field behaviour and plasma cholinesterase activity in male, female and masculinized female rats
title_full Developmental expression of sexual differences in open-field behaviour and plasma cholinesterase activity in male, female and masculinized female rats
title_fullStr Developmental expression of sexual differences in open-field behaviour and plasma cholinesterase activity in male, female and masculinized female rats
title_full_unstemmed Developmental expression of sexual differences in open-field behaviour and plasma cholinesterase activity in male, female and masculinized female rats
title_sort Developmental expression of sexual differences in open-field behaviour and plasma cholinesterase activity in male, female and masculinized female rats
author Felicio, Luciano Freitas
author_facet Felicio, Luciano Freitas
Spinosa, Helenice de Souza
Rocha, Luiz Carlos de Sa
Hucke, Erica Engelberg Teixeira da Silva
author_role author
author2 Spinosa, Helenice de Souza
Rocha, Luiz Carlos de Sa
Hucke, Erica Engelberg Teixeira da Silva
author2_role author
author
author
dc.contributor.author.fl_str_mv Felicio, Luciano Freitas
Spinosa, Helenice de Souza
Rocha, Luiz Carlos de Sa
Hucke, Erica Engelberg Teixeira da Silva
dc.subject.por.fl_str_mv Características sexuais
Colinesterase
Testosterona
Comportamento animal
Sex characteristic
Cholinesterase
Testosterone
Animal behavior
topic Características sexuais
Colinesterase
Testosterona
Comportamento animal
Sex characteristic
Cholinesterase
Testosterone
Animal behavior
description Sexual differences in behaviour and metabolism are well recognized. While some of these differences are related to testosterone exposure during neonatal life, others do not depend on the organizational action of androgens during early development. The objective of the following experiments was to study the development of sexual differences in plasma cholinesterase activity and to determine if these differences were related to testosterone exposure postnatally. Open-field activity was also recorded as a behavioral indicator of the actions of testosterone on sexual differentiation of the central nervous system. Three treatment groups of animals were used: normal male, normal female, and masculinized female rats (1 mg testosterone, SC, on day 2 of postnatal life). Open-field behaviour was measured on three consecutive days just after weaning (21 -23 days of age), in association with the onset of puberty (30 - 36 days of age), or as adults (90 - 110 days of age); plasma cholinesterase activity was measured at 22, 30 - 36, and 90 - 110 days of age. As expected a sex difference in open-field activity was found between normal males and females. Postnatal androgen treatment in females decreased open-field activity in adulthood to levels similar to those found in normal males. Similar differences were observed just after weaning, but not at 30-36 days of age. In contrast, significant differences in cholinesterase activity were observed in adult animals, but not at days 22 and 30 - 33 of age. Masculinized female rats showed no differences in plasma cholinesterase activity when compared to normal female rats, both groups differing from males. These data suggest that sexual differences in plasma cholinesterase activity in adult rats, unlike differences in open-field behaviour, are not dependent on testosterone exposure during postnatal life. In addition, the results have shown that under stress (weaning) sex differences in open-field behaviour can be observed as early as at 21 -23 days of age.
publishDate 1995
dc.date.none.fl_str_mv 1995-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/52082
10.11606/issn.1678-4456.bjvras.1994.52082
url https://www.revistas.usp.br/bjvras/article/view/52082
identifier_str_mv 10.11606/issn.1678-4456.bjvras.1994.52082
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/52082/56132
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 32 Núm. 1 (1995); 5-10
Brazilian Journal of Veterinary Research and Animal Science; Vol. 32 No. 1 (1995); 5-10
Brazilian Journal of Veterinary Research and Animal Science; v. 32 n. 1 (1995); 5-10
Brazilian Journal of Veterinary Research and Animal Science; V. 32 N. 1 (1995); 5-10
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
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