Sheep erythrocyte sensitisation and restriction to lysis by the complement system from equine and sheep serum

Detalhes bibliográficos
Autor(a) principal: Pereira, Gislene
Data de Publicação: 1995
Outros Autores: Valim, Yara Maria Lucisano, Azzolini, Ana Elisa Caleiro Seixas, Pandochi, Ana Isabel de Assis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
Texto Completo: https://www.revistas.usp.br/bjvras/article/view/52102
Resumo: The effect of the degree of sheep red cell sensitisation by antibody in the resistance to lysis by sheep and horse complement was analysed. The results showed that, as the level of sensitisation increased, so did the homologous complement activity, eventually leading to one hundred percent of lysis; the same was observed for horse serum, generally considered as not efficient at lysing these cells. Therefore, the homologous and heterologous restriction can be overridden by this treatment. This may be of interest for the comprehension of the mechanisms involved in the modulation of the restriction phenomenon in health an in diseases involving complement in domestic animals and in human, as well as in laboratory diagnosis where hemolytic assays are used for measurement of complement activity and evaluation of presence of antibodies in serum samples.
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spelling Sheep erythrocyte sensitisation and restriction to lysis by the complement system from equine and sheep serumSensibilização de hemácias de carneiro e restrição à lise pelo sistema complemento do soro eqüino e de carneiroLysisErythrocytesAntibodiesHemolysisLiseEritrócitosAnticorposHemóliseThe effect of the degree of sheep red cell sensitisation by antibody in the resistance to lysis by sheep and horse complement was analysed. The results showed that, as the level of sensitisation increased, so did the homologous complement activity, eventually leading to one hundred percent of lysis; the same was observed for horse serum, generally considered as not efficient at lysing these cells. Therefore, the homologous and heterologous restriction can be overridden by this treatment. This may be of interest for the comprehension of the mechanisms involved in the modulation of the restriction phenomenon in health an in diseases involving complement in domestic animals and in human, as well as in laboratory diagnosis where hemolytic assays are used for measurement of complement activity and evaluation of presence of antibodies in serum samples.Neste trabalho foi analisado o efeito do grau de sensibilização de hemácias de carneiro por anticorpo, na resistência à lise pelo complemento do soro eqüino e de carneiro. Os resultados mostraram que, à medida que houve um aumento do nível de sensibilização, ocorreu também um aumento da atividade do complemento homólogo, levando a 100% de lise; o mesmo foi observado para o complemento do soro eqüino, geralmente considerado ineficiente na lise destas células. A restrição à lise pode, assim, ser sobrepujada por este tratamento. Estes dados podem ser de interesse para a compreensão dos mecanismos envolvidos na modulação do fenômeno de restrição, na saúde e em doenças envolvendo o sistema complemento em animais domésticos e no homem, bem como em diagnóstico laboratorial, onde são utilizados ensaios hemolíticos para medida de atividade do complemento e pesquisa de anticorpos em amostras de soro.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia1995-09-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/5210210.11606/issn.1678-4456.bjvras.1994.52102Brazilian Journal of Veterinary Research and Animal Science; Vol. 32 Núm. 3 (1995); 155-159Brazilian Journal of Veterinary Research and Animal Science; Vol. 32 No. 3 (1995); 155-159Brazilian Journal of Veterinary Research and Animal Science; v. 32 n. 3 (1995); 155-159Brazilian Journal of Veterinary Research and Animal Science; V. 32 N. 3 (1995); 155-1591678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/52102/56152Pereira, GisleneValim, Yara Maria LucisanoAzzolini, Ana Elisa Caleiro SeixasPandochi, Ana Isabel de Assisinfo:eu-repo/semantics/openAccess2020-06-23T04:51:16Zoai:revistas.usp.br:article/52102Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:30.379026Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv Sheep erythrocyte sensitisation and restriction to lysis by the complement system from equine and sheep serum
Sensibilização de hemácias de carneiro e restrição à lise pelo sistema complemento do soro eqüino e de carneiro
title Sheep erythrocyte sensitisation and restriction to lysis by the complement system from equine and sheep serum
spellingShingle Sheep erythrocyte sensitisation and restriction to lysis by the complement system from equine and sheep serum
Pereira, Gislene
Lysis
Erythrocytes
Antibodies
Hemolysis
Lise
Eritrócitos
Anticorpos
Hemólise
title_short Sheep erythrocyte sensitisation and restriction to lysis by the complement system from equine and sheep serum
title_full Sheep erythrocyte sensitisation and restriction to lysis by the complement system from equine and sheep serum
title_fullStr Sheep erythrocyte sensitisation and restriction to lysis by the complement system from equine and sheep serum
title_full_unstemmed Sheep erythrocyte sensitisation and restriction to lysis by the complement system from equine and sheep serum
title_sort Sheep erythrocyte sensitisation and restriction to lysis by the complement system from equine and sheep serum
author Pereira, Gislene
author_facet Pereira, Gislene
Valim, Yara Maria Lucisano
Azzolini, Ana Elisa Caleiro Seixas
Pandochi, Ana Isabel de Assis
author_role author
author2 Valim, Yara Maria Lucisano
Azzolini, Ana Elisa Caleiro Seixas
Pandochi, Ana Isabel de Assis
author2_role author
author
author
dc.contributor.author.fl_str_mv Pereira, Gislene
Valim, Yara Maria Lucisano
Azzolini, Ana Elisa Caleiro Seixas
Pandochi, Ana Isabel de Assis
dc.subject.por.fl_str_mv Lysis
Erythrocytes
Antibodies
Hemolysis
Lise
Eritrócitos
Anticorpos
Hemólise
topic Lysis
Erythrocytes
Antibodies
Hemolysis
Lise
Eritrócitos
Anticorpos
Hemólise
description The effect of the degree of sheep red cell sensitisation by antibody in the resistance to lysis by sheep and horse complement was analysed. The results showed that, as the level of sensitisation increased, so did the homologous complement activity, eventually leading to one hundred percent of lysis; the same was observed for horse serum, generally considered as not efficient at lysing these cells. Therefore, the homologous and heterologous restriction can be overridden by this treatment. This may be of interest for the comprehension of the mechanisms involved in the modulation of the restriction phenomenon in health an in diseases involving complement in domestic animals and in human, as well as in laboratory diagnosis where hemolytic assays are used for measurement of complement activity and evaluation of presence of antibodies in serum samples.
publishDate 1995
dc.date.none.fl_str_mv 1995-09-03
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/52102
10.11606/issn.1678-4456.bjvras.1994.52102
url https://www.revistas.usp.br/bjvras/article/view/52102
identifier_str_mv 10.11606/issn.1678-4456.bjvras.1994.52102
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/52102/56152
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 32 Núm. 3 (1995); 155-159
Brazilian Journal of Veterinary Research and Animal Science; Vol. 32 No. 3 (1995); 155-159
Brazilian Journal of Veterinary Research and Animal Science; v. 32 n. 3 (1995); 155-159
Brazilian Journal of Veterinary Research and Animal Science; V. 32 N. 3 (1995); 155-159
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
_version_ 1797051564304629760

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