Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells

Detalhes bibliográficos
Autor(a) principal: Laurenti, Márcia Dalastra
Data de Publicação: 2005
Outros Autores: Örn, Andres, Sinhorini, Idércio Luiz, Corbett, Carlos Eduardo Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
DOI: 10.11606/issn.1678-4456.bjvras.2005.26440
Texto Completo: https://www.revistas.usp.br/bjvras/article/view/26440
Resumo: BALBI c mice depleted and non-depleted of Natural Killer (NK) cells were infected subcutaneously with 107 stationary phase promastigotes of Leishmania (Leishmania) amazonensis and samples were taken at 24 hours and 7 days after infection. In NK cell-depleted mice, the NK cytotoxic activity of spleen cells decreased at 7 days after infection and more parasites were found in the lesion. The NK cell populations were analyzed by immunohistochemistry in spleen cryosections. An increase of NK1.1+ expression and a decrease of NK5E6+ antigen expression was observed in NK cell-depleted mice compared to non-depleted mice. When the presence of IFN-g, IL-12 and IL-4 at the site of parasite inoculation was analyzed by immunohistochemistry, a large amount of cytokines was detected in K cell-depleted mice at 24 hours and 7 days after infection. In non-depleted mice, there was a small amount of IL-12 at 24 hours and of IL-4 at 7 days after infection. These data cells suggest that K cell depletion by 90Srresults in increased parasitism in the lesion. The increase of NK1.1+ expression, which mainly produces IL-4, may take part in the progression of the infection.
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spelling Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cellsDetecção de citocinas no sítio de inoculação subcutânea de Leishmania (Leishmania) amazonensis em camundongos depletados de células Natural KillerCélulas Natural KillerCitocinasLeishmaniose cutâneaLeishmania (Leishmania) amazonensisImunopatologia90 EstrôncioNatural Killer cellsCytokinesCutaneous LeishmaniasisLeishmania (Leishmania) amazonensisImmunopathology90 Strontium BALBI c mice depleted and non-depleted of Natural Killer (NK) cells were infected subcutaneously with 107 stationary phase promastigotes of Leishmania (Leishmania) amazonensis and samples were taken at 24 hours and 7 days after infection. In NK cell-depleted mice, the NK cytotoxic activity of spleen cells decreased at 7 days after infection and more parasites were found in the lesion. The NK cell populations were analyzed by immunohistochemistry in spleen cryosections. An increase of NK1.1+ expression and a decrease of NK5E6+ antigen expression was observed in NK cell-depleted mice compared to non-depleted mice. When the presence of IFN-g, IL-12 and IL-4 at the site of parasite inoculation was analyzed by immunohistochemistry, a large amount of cytokines was detected in K cell-depleted mice at 24 hours and 7 days after infection. In non-depleted mice, there was a small amount of IL-12 at 24 hours and of IL-4 at 7 days after infection. These data cells suggest that K cell depletion by 90Srresults in increased parasitism in the lesion. The increase of NK1.1+ expression, which mainly produces IL-4, may take part in the progression of the infection. Camundongos BALB/ c depletados e não depletados em células Natural IVller (NK) foram infectados subcutaneamente com 107 promastigotas de Leishmania (Leishmania) amazonensis em fase estacionária de crescimento e amostras foram colhidas às 24 horas e 7 dias de infecção. Nos camundongos depletados em células NK, a atividade citotóxica NK de células esplênicas estava diminuída aos 7 dias de infecção e mais parasitos foram encontrados na lesão. Populações de células NK foram analisadas por imuno-histoquimica em cortes congelados de baço. Foi observado aumento na expressão de células NK1.1+ e diminuição na expressão do antígeno NK5E6+ nos animais depletados em células NK comparados aos camundongos não depletados. A presença de IFN-g, IL-12 e IL-4, analisada por imunohistoquimica no sítio de inoculação dos parasitos, mostrou que maior quantidade de citocinas foram detectadas nos camundongos depletados em células NK às 24 horas e 7 dias depois da infecção. Nos camundongos não depletados, havia pequenas quantidades de IL-12 às 24 horas e de IL-4 aos 7 dias de infecção. Estes dados sugerem que a depleção de células NK por 90Sr resulta em aumento de parasitismo na lesão. O aumento na expressão de células NK1.1+, as quais produzem principalmente IL-4, pode representar um dos mecanismos que colaborariam para a progressão da infecção. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2005-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/2644010.11606/issn.1678-4456.bjvras.2005.26440Brazilian Journal of Veterinary Research and Animal Science; Vol. 42 Núm. 2 (2005); 105-112Brazilian Journal of Veterinary Research and Animal Science; Vol. 42 No. 2 (2005); 105-112Brazilian Journal of Veterinary Research and Animal Science; v. 42 n. 2 (2005); 105-112Brazilian Journal of Veterinary Research and Animal Science; V. 42 N. 2 (2005); 105-1121678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/26440/62080Laurenti, Márcia DalastraÖrn, AndresSinhorini, Idércio LuizCorbett, Carlos Eduardo Pereirainfo:eu-repo/semantics/openAccess2020-06-23T04:20:24Zoai:revistas.usp.br:article/26440Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:43.730896Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells
Detecção de citocinas no sítio de inoculação subcutânea de Leishmania (Leishmania) amazonensis em camundongos depletados de células Natural Killer
title Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells
spellingShingle Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells
Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells
Laurenti, Márcia Dalastra
Células Natural Killer
Citocinas
Leishmaniose cutânea
Leishmania (Leishmania) amazonensis
Imunopatologia
90 Estrôncio
Natural Killer cells
Cytokines
Cutaneous Leishmaniasis
Leishmania (Leishmania) amazonensis
Immunopathology
90 Strontium
Laurenti, Márcia Dalastra
Células Natural Killer
Citocinas
Leishmaniose cutânea
Leishmania (Leishmania) amazonensis
Imunopatologia
90 Estrôncio
Natural Killer cells
Cytokines
Cutaneous Leishmaniasis
Leishmania (Leishmania) amazonensis
Immunopathology
90 Strontium
title_short Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells
title_full Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells
title_fullStr Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells
Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells
title_full_unstemmed Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells
Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells
title_sort Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells
author Laurenti, Márcia Dalastra
author_facet Laurenti, Márcia Dalastra
Laurenti, Márcia Dalastra
Örn, Andres
Sinhorini, Idércio Luiz
Corbett, Carlos Eduardo Pereira
Örn, Andres
Sinhorini, Idércio Luiz
Corbett, Carlos Eduardo Pereira
author_role author
author2 Örn, Andres
Sinhorini, Idércio Luiz
Corbett, Carlos Eduardo Pereira
author2_role author
author
author
dc.contributor.author.fl_str_mv Laurenti, Márcia Dalastra
Örn, Andres
Sinhorini, Idércio Luiz
Corbett, Carlos Eduardo Pereira
dc.subject.por.fl_str_mv Células Natural Killer
Citocinas
Leishmaniose cutânea
Leishmania (Leishmania) amazonensis
Imunopatologia
90 Estrôncio
Natural Killer cells
Cytokines
Cutaneous Leishmaniasis
Leishmania (Leishmania) amazonensis
Immunopathology
90 Strontium
topic Células Natural Killer
Citocinas
Leishmaniose cutânea
Leishmania (Leishmania) amazonensis
Imunopatologia
90 Estrôncio
Natural Killer cells
Cytokines
Cutaneous Leishmaniasis
Leishmania (Leishmania) amazonensis
Immunopathology
90 Strontium
description BALBI c mice depleted and non-depleted of Natural Killer (NK) cells were infected subcutaneously with 107 stationary phase promastigotes of Leishmania (Leishmania) amazonensis and samples were taken at 24 hours and 7 days after infection. In NK cell-depleted mice, the NK cytotoxic activity of spleen cells decreased at 7 days after infection and more parasites were found in the lesion. The NK cell populations were analyzed by immunohistochemistry in spleen cryosections. An increase of NK1.1+ expression and a decrease of NK5E6+ antigen expression was observed in NK cell-depleted mice compared to non-depleted mice. When the presence of IFN-g, IL-12 and IL-4 at the site of parasite inoculation was analyzed by immunohistochemistry, a large amount of cytokines was detected in K cell-depleted mice at 24 hours and 7 days after infection. In non-depleted mice, there was a small amount of IL-12 at 24 hours and of IL-4 at 7 days after infection. These data cells suggest that K cell depletion by 90Srresults in increased parasitism in the lesion. The increase of NK1.1+ expression, which mainly produces IL-4, may take part in the progression of the infection.
publishDate 2005
dc.date.none.fl_str_mv 2005-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/26440
10.11606/issn.1678-4456.bjvras.2005.26440
url https://www.revistas.usp.br/bjvras/article/view/26440
identifier_str_mv 10.11606/issn.1678-4456.bjvras.2005.26440
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/26440/62080
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 42 Núm. 2 (2005); 105-112
Brazilian Journal of Veterinary Research and Animal Science; Vol. 42 No. 2 (2005); 105-112
Brazilian Journal of Veterinary Research and Animal Science; v. 42 n. 2 (2005); 105-112
Brazilian Journal of Veterinary Research and Animal Science; V. 42 N. 2 (2005); 105-112
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
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dc.identifier.doi.none.fl_str_mv 10.11606/issn.1678-4456.bjvras.2005.26440