Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
DOI: | 10.11606/issn.1678-4456.bjvras.2005.26440 |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/26440 |
Resumo: | BALBI c mice depleted and non-depleted of Natural Killer (NK) cells were infected subcutaneously with 107 stationary phase promastigotes of Leishmania (Leishmania) amazonensis and samples were taken at 24 hours and 7 days after infection. In NK cell-depleted mice, the NK cytotoxic activity of spleen cells decreased at 7 days after infection and more parasites were found in the lesion. The NK cell populations were analyzed by immunohistochemistry in spleen cryosections. An increase of NK1.1+ expression and a decrease of NK5E6+ antigen expression was observed in NK cell-depleted mice compared to non-depleted mice. When the presence of IFN-g, IL-12 and IL-4 at the site of parasite inoculation was analyzed by immunohistochemistry, a large amount of cytokines was detected in K cell-depleted mice at 24 hours and 7 days after infection. In non-depleted mice, there was a small amount of IL-12 at 24 hours and of IL-4 at 7 days after infection. These data cells suggest that K cell depletion by 90Srresults in increased parasitism in the lesion. The increase of NK1.1+ expression, which mainly produces IL-4, may take part in the progression of the infection. |
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Brazilian Journal of Veterinary Research and Animal Science |
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Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cellsDetecção de citocinas no sítio de inoculação subcutânea de Leishmania (Leishmania) amazonensis em camundongos depletados de células Natural KillerCélulas Natural KillerCitocinasLeishmaniose cutâneaLeishmania (Leishmania) amazonensisImunopatologia90 EstrôncioNatural Killer cellsCytokinesCutaneous LeishmaniasisLeishmania (Leishmania) amazonensisImmunopathology90 Strontium BALBI c mice depleted and non-depleted of Natural Killer (NK) cells were infected subcutaneously with 107 stationary phase promastigotes of Leishmania (Leishmania) amazonensis and samples were taken at 24 hours and 7 days after infection. In NK cell-depleted mice, the NK cytotoxic activity of spleen cells decreased at 7 days after infection and more parasites were found in the lesion. The NK cell populations were analyzed by immunohistochemistry in spleen cryosections. An increase of NK1.1+ expression and a decrease of NK5E6+ antigen expression was observed in NK cell-depleted mice compared to non-depleted mice. When the presence of IFN-g, IL-12 and IL-4 at the site of parasite inoculation was analyzed by immunohistochemistry, a large amount of cytokines was detected in K cell-depleted mice at 24 hours and 7 days after infection. In non-depleted mice, there was a small amount of IL-12 at 24 hours and of IL-4 at 7 days after infection. These data cells suggest that K cell depletion by 90Srresults in increased parasitism in the lesion. The increase of NK1.1+ expression, which mainly produces IL-4, may take part in the progression of the infection. Camundongos BALB/ c depletados e não depletados em células Natural IVller (NK) foram infectados subcutaneamente com 107 promastigotas de Leishmania (Leishmania) amazonensis em fase estacionária de crescimento e amostras foram colhidas às 24 horas e 7 dias de infecção. Nos camundongos depletados em células NK, a atividade citotóxica NK de células esplênicas estava diminuída aos 7 dias de infecção e mais parasitos foram encontrados na lesão. Populações de células NK foram analisadas por imuno-histoquimica em cortes congelados de baço. Foi observado aumento na expressão de células NK1.1+ e diminuição na expressão do antígeno NK5E6+ nos animais depletados em células NK comparados aos camundongos não depletados. A presença de IFN-g, IL-12 e IL-4, analisada por imunohistoquimica no sítio de inoculação dos parasitos, mostrou que maior quantidade de citocinas foram detectadas nos camundongos depletados em células NK às 24 horas e 7 dias depois da infecção. Nos camundongos não depletados, havia pequenas quantidades de IL-12 às 24 horas e de IL-4 aos 7 dias de infecção. Estes dados sugerem que a depleção de células NK por 90Sr resulta em aumento de parasitismo na lesão. O aumento na expressão de células NK1.1+, as quais produzem principalmente IL-4, pode representar um dos mecanismos que colaborariam para a progressão da infecção. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2005-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/2644010.11606/issn.1678-4456.bjvras.2005.26440Brazilian Journal of Veterinary Research and Animal Science; Vol. 42 Núm. 2 (2005); 105-112Brazilian Journal of Veterinary Research and Animal Science; Vol. 42 No. 2 (2005); 105-112Brazilian Journal of Veterinary Research and Animal Science; v. 42 n. 2 (2005); 105-112Brazilian Journal of Veterinary Research and Animal Science; V. 42 N. 2 (2005); 105-1121678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/26440/62080Laurenti, Márcia DalastraÖrn, AndresSinhorini, Idércio LuizCorbett, Carlos Eduardo Pereirainfo:eu-repo/semantics/openAccess2020-06-23T04:20:24Zoai:revistas.usp.br:article/26440Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:43.730896Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells Detecção de citocinas no sítio de inoculação subcutânea de Leishmania (Leishmania) amazonensis em camundongos depletados de células Natural Killer |
title |
Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells |
spellingShingle |
Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells Laurenti, Márcia Dalastra Células Natural Killer Citocinas Leishmaniose cutânea Leishmania (Leishmania) amazonensis Imunopatologia 90 Estrôncio Natural Killer cells Cytokines Cutaneous Leishmaniasis Leishmania (Leishmania) amazonensis Immunopathology 90 Strontium Laurenti, Márcia Dalastra Células Natural Killer Citocinas Leishmaniose cutânea Leishmania (Leishmania) amazonensis Imunopatologia 90 Estrôncio Natural Killer cells Cytokines Cutaneous Leishmaniasis Leishmania (Leishmania) amazonensis Immunopathology 90 Strontium |
title_short |
Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells |
title_full |
Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells |
title_fullStr |
Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells |
title_full_unstemmed |
Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells |
title_sort |
Cytokine detection at the site of l.(l.) Amazonensis subcutaneous inoculation in mice depleted of Natural Killer cells |
author |
Laurenti, Márcia Dalastra |
author_facet |
Laurenti, Márcia Dalastra Laurenti, Márcia Dalastra Örn, Andres Sinhorini, Idércio Luiz Corbett, Carlos Eduardo Pereira Örn, Andres Sinhorini, Idércio Luiz Corbett, Carlos Eduardo Pereira |
author_role |
author |
author2 |
Örn, Andres Sinhorini, Idércio Luiz Corbett, Carlos Eduardo Pereira |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Laurenti, Márcia Dalastra Örn, Andres Sinhorini, Idércio Luiz Corbett, Carlos Eduardo Pereira |
dc.subject.por.fl_str_mv |
Células Natural Killer Citocinas Leishmaniose cutânea Leishmania (Leishmania) amazonensis Imunopatologia 90 Estrôncio Natural Killer cells Cytokines Cutaneous Leishmaniasis Leishmania (Leishmania) amazonensis Immunopathology 90 Strontium |
topic |
Células Natural Killer Citocinas Leishmaniose cutânea Leishmania (Leishmania) amazonensis Imunopatologia 90 Estrôncio Natural Killer cells Cytokines Cutaneous Leishmaniasis Leishmania (Leishmania) amazonensis Immunopathology 90 Strontium |
description |
BALBI c mice depleted and non-depleted of Natural Killer (NK) cells were infected subcutaneously with 107 stationary phase promastigotes of Leishmania (Leishmania) amazonensis and samples were taken at 24 hours and 7 days after infection. In NK cell-depleted mice, the NK cytotoxic activity of spleen cells decreased at 7 days after infection and more parasites were found in the lesion. The NK cell populations were analyzed by immunohistochemistry in spleen cryosections. An increase of NK1.1+ expression and a decrease of NK5E6+ antigen expression was observed in NK cell-depleted mice compared to non-depleted mice. When the presence of IFN-g, IL-12 and IL-4 at the site of parasite inoculation was analyzed by immunohistochemistry, a large amount of cytokines was detected in K cell-depleted mice at 24 hours and 7 days after infection. In non-depleted mice, there was a small amount of IL-12 at 24 hours and of IL-4 at 7 days after infection. These data cells suggest that K cell depletion by 90Srresults in increased parasitism in the lesion. The increase of NK1.1+ expression, which mainly produces IL-4, may take part in the progression of the infection. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/26440 10.11606/issn.1678-4456.bjvras.2005.26440 |
url |
https://www.revistas.usp.br/bjvras/article/view/26440 |
identifier_str_mv |
10.11606/issn.1678-4456.bjvras.2005.26440 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/26440/62080 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 42 Núm. 2 (2005); 105-112 Brazilian Journal of Veterinary Research and Animal Science; Vol. 42 No. 2 (2005); 105-112 Brazilian Journal of Veterinary Research and Animal Science; v. 42 n. 2 (2005); 105-112 Brazilian Journal of Veterinary Research and Animal Science; V. 42 N. 2 (2005); 105-112 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1822182143768395776 |
dc.identifier.doi.none.fl_str_mv |
10.11606/issn.1678-4456.bjvras.2005.26440 |