Use of spermatocrit to estimate sperm concentration of semen from piabanha (Brycon insignis)

Detalhes bibliográficos
Autor(a) principal: Shimoda, Eduardo
Data de Publicação: 2007
Outros Autores: Andrade, Dalcio Ricardo de, Vidal Junior, Manuel Vazquez, Yasui, George Shigueki, Godinho, Hugo Pereira, Silva, José Frederico Straggiotti, Sousa, Guilherme
Tipo de documento: Artigo
Idioma: por
Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
Texto Completo: https://www.revistas.usp.br/bjvras/article/view/26585
Resumo: The aim of this work was to determine the viability of spermatocrit use in Piabanha's sperm concentration estimation. Gametogenesis of piabanha Brycon insignis (n=29) was induced with crude pituitary extract of carp and semen was further collected. The spermatocrit was determined using cappilar tube micro-centrifuge. The sperm concentration was verified by counting in "Neubauer chamber", after dilution. The verified sperm concentration (mean±standard deviation) was 24,38±3,84x10(9) spermatozoa/mL, and the spermatocrit was 16,14±5,20%. The regression model: v = 14,01.10(9) + 0,6428.10(9).X was significant (P<0,01) and presented adjusted R² = 0,75. Thus, the estimation of sperm concentration using the spermatocrit is possible.
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spelling Use of spermatocrit to estimate sperm concentration of semen from piabanha (Brycon insignis)Utilização do espermatócrito para estimar a concentração espermática no sêmen da piabanha (Brycon insignis)Concentração espermáticaEspermatócritoAnálise seminalPiabanhaSperm concentrationEspermatocritSeminal analysisPiabanhaThe aim of this work was to determine the viability of spermatocrit use in Piabanha's sperm concentration estimation. Gametogenesis of piabanha Brycon insignis (n=29) was induced with crude pituitary extract of carp and semen was further collected. The spermatocrit was determined using cappilar tube micro-centrifuge. The sperm concentration was verified by counting in "Neubauer chamber", after dilution. The verified sperm concentration (mean±standard deviation) was 24,38±3,84x10(9) spermatozoa/mL, and the spermatocrit was 16,14±5,20%. The regression model: v = 14,01.10(9) + 0,6428.10(9).X was significant (P<0,01) and presented adjusted R² = 0,75. Thus, the estimation of sperm concentration using the spermatocrit is possible.O objetivo deste trabalho foi verificar a viabilidade da utilização do espermatócrito para estimar a concentração espermática do sêmen de piabanha. Os exemplares (n=29) de piabanha Brycon insignis foram hipofisados, sendo posteriormente realizada a coleta de sêmen. O espermatócrito foi determinado utilizando-se micro-centrífuga de tubos capilares. A concentração espermática foi verificada por contagem em câmara de Neubauer, após diluição. A concentração espermática verificada (média±desvio padrão) foi de 24,38 ± 3,84 x 10(9) espermatozóides / mL, e o espermatócrito foi de 16,14±5,20%. A equação de regressão: v = 14,01.10(9) + 0,6428.10(9).X foi significativa (P<0,01) e apresentou R² ajustado = 0,75. Deste modo, é possível estimar a concentração através da determinação do espermatócrito.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2007-12-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/2658510.11606/issn.1678-4456.bjvras.2007.26585Brazilian Journal of Veterinary Research and Animal Science; Vol. 44 (2007): Suplemento; 19-24Brazilian Journal of Veterinary Research and Animal Science; Vol. 44 (2007): Suplemento; 19-24Brazilian Journal of Veterinary Research and Animal Science; v. 44 (2007): Suplemento; 19-24Brazilian Journal of Veterinary Research and Animal Science; V. 44 (2007): Suplemento; 19-241678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/26585/28368Shimoda, EduardoAndrade, Dalcio Ricardo deVidal Junior, Manuel VazquezYasui, George ShiguekiGodinho, Hugo PereiraSilva, José Frederico StraggiottiSousa, Guilhermeinfo:eu-repo/semantics/openAccess2020-06-23T04:14:24Zoai:revistas.usp.br:article/26585Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:51.854968Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv Use of spermatocrit to estimate sperm concentration of semen from piabanha (Brycon insignis)
Utilização do espermatócrito para estimar a concentração espermática no sêmen da piabanha (Brycon insignis)
title Use of spermatocrit to estimate sperm concentration of semen from piabanha (Brycon insignis)
spellingShingle Use of spermatocrit to estimate sperm concentration of semen from piabanha (Brycon insignis)
Shimoda, Eduardo
Concentração espermática
Espermatócrito
Análise seminal
Piabanha
Sperm concentration
Espermatocrit
Seminal analysis
Piabanha
title_short Use of spermatocrit to estimate sperm concentration of semen from piabanha (Brycon insignis)
title_full Use of spermatocrit to estimate sperm concentration of semen from piabanha (Brycon insignis)
title_fullStr Use of spermatocrit to estimate sperm concentration of semen from piabanha (Brycon insignis)
title_full_unstemmed Use of spermatocrit to estimate sperm concentration of semen from piabanha (Brycon insignis)
title_sort Use of spermatocrit to estimate sperm concentration of semen from piabanha (Brycon insignis)
author Shimoda, Eduardo
author_facet Shimoda, Eduardo
Andrade, Dalcio Ricardo de
Vidal Junior, Manuel Vazquez
Yasui, George Shigueki
Godinho, Hugo Pereira
Silva, José Frederico Straggiotti
Sousa, Guilherme
author_role author
author2 Andrade, Dalcio Ricardo de
Vidal Junior, Manuel Vazquez
Yasui, George Shigueki
Godinho, Hugo Pereira
Silva, José Frederico Straggiotti
Sousa, Guilherme
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Shimoda, Eduardo
Andrade, Dalcio Ricardo de
Vidal Junior, Manuel Vazquez
Yasui, George Shigueki
Godinho, Hugo Pereira
Silva, José Frederico Straggiotti
Sousa, Guilherme
dc.subject.por.fl_str_mv Concentração espermática
Espermatócrito
Análise seminal
Piabanha
Sperm concentration
Espermatocrit
Seminal analysis
Piabanha
topic Concentração espermática
Espermatócrito
Análise seminal
Piabanha
Sperm concentration
Espermatocrit
Seminal analysis
Piabanha
description The aim of this work was to determine the viability of spermatocrit use in Piabanha's sperm concentration estimation. Gametogenesis of piabanha Brycon insignis (n=29) was induced with crude pituitary extract of carp and semen was further collected. The spermatocrit was determined using cappilar tube micro-centrifuge. The sperm concentration was verified by counting in "Neubauer chamber", after dilution. The verified sperm concentration (mean±standard deviation) was 24,38±3,84x10(9) spermatozoa/mL, and the spermatocrit was 16,14±5,20%. The regression model: v = 14,01.10(9) + 0,6428.10(9).X was significant (P<0,01) and presented adjusted R² = 0,75. Thus, the estimation of sperm concentration using the spermatocrit is possible.
publishDate 2007
dc.date.none.fl_str_mv 2007-12-03
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/26585
10.11606/issn.1678-4456.bjvras.2007.26585
url https://www.revistas.usp.br/bjvras/article/view/26585
identifier_str_mv 10.11606/issn.1678-4456.bjvras.2007.26585
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/26585/28368
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 44 (2007): Suplemento; 19-24
Brazilian Journal of Veterinary Research and Animal Science; Vol. 44 (2007): Suplemento; 19-24
Brazilian Journal of Veterinary Research and Animal Science; v. 44 (2007): Suplemento; 19-24
Brazilian Journal of Veterinary Research and Animal Science; V. 44 (2007): Suplemento; 19-24
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
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