Development of an experimental model of neutrophilic pulmonary response induction in mice

• Autor(a) principal: Pinto,Leonardo Araújo
• Data de Publicação: 2003
• Outros Autores: Camozzato,Camila, Avozani,Monique, Machado,Denise Cantarelli, Jones,Marcus Herbert, Stein,Renato Tetelbom, Pitrez,Paulo Márcio Condessa
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Jornal de Pneumologia
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-35862003000400009
• topic: Animal experimentation; Lung disesases; Inflammation; Neutrophils; Pseudomonas

BACKGROUND: Several lung diseases are characterized by a predominantly neutrophilic inflammation. A better understanding of the mechanisms of action of some drugs on the airway inflammation of such diseases may bring advances to the treatment. OBJECTIVE: To develop a method to induce pulmonary neutrophilic response in mice, without active infection. METHODS: Eight adult Swiss mice were used. The study group (n = 4) received an intranasal challenge with 1 x 10(12) CFU/ml of Pseudomonas aeruginosa (Psa), frozen to death. The control group (n = 4) received an intranasal challenge with saline solution. Two days after the intranasal challenge, a bron­choalveolar lavage (BAL) was performed with total cell and differential cellularity counts. RESULTS: The total cell count was significantly higher in the group with Psa, as compared to the control group (median of 1.17 x 10(6) and 0.08 x 10(6), respectively, p = 0.029). In addition to this, an absolute predominance of neutrophils was found in the differential cellularity of the mice that had received the Psa challenge. CONCLUSIONS: The model of inducing a neutrophilic pulmonary disease using frost-dead bacteria was successfully developed. This neutrophilic inflammatory response induction model in Swiss mice lungs may be an important tool for testing the anti-inflammatory effect of some antimicrobial drugs on the inflammation of the lower airways.