Effect of clarithromycin on the cell profile of bronchoalveolar lavage fluid in mice with neutrophil-predominant lung disease

Detalhes bibliográficos
Autor(a) principal: Pinto,Leonardo Araújo
Data de Publicação: 2004
Outros Autores: Camozzato,Camila, Avozani,Monique, Machado,Denise Cantarelli, Jones,Marcus Herbert, Stein,Renato Tetelbom, Pitrez,Paulo Márcio Condessa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Hospital das Clínicas
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0041-87812004000300002
Resumo: OBJECTIVE: Macrolide antibiotics have anti-inflammatory properties in lung diseases. The aim of this study was to investigate the effect of clarithromycin in pulmonary cellular inflammatory response in mice. METHOD: Eight adult Swiss mice were studied. All animals received an intranasal challenge (80 µL) with dead Pseudomonas aeruginosa (1.0 x 10(12) CFU/mL). Bronchoalveolar lavage was performed 2 days later, with total cell count and differential cell analysis. The study group (n = 4) received clarithromycin treatment (50 mg/kg/day, intraperitoneal) for 5 days. Treatment was initiated 2 days before intranasal challenge. RESULTS: There was no significant difference in total cell count between the groups (mean: 2.0 x 10(6) and 1.3 x 10(6), respectively). In both groups, there was a predominance of neutrophils. However, the study group had a higher percentage of lymphocytes in the bronchoalveolar lavage than the control group (median of 19% vs 2.5%, P = .029). CONCLUSION: Clarithromycin alters the cytological pattern of bronchoalveolar lavage of Swiss mice with neutrophil pulmonary inflammation, significantly increasing the percentage of lymphocytes.
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spelling Effect of clarithromycin on the cell profile of bronchoalveolar lavage fluid in mice with neutrophil-predominant lung diseaseClarithromycinLung inflammationLungMiceNeutrophilOBJECTIVE: Macrolide antibiotics have anti-inflammatory properties in lung diseases. The aim of this study was to investigate the effect of clarithromycin in pulmonary cellular inflammatory response in mice. METHOD: Eight adult Swiss mice were studied. All animals received an intranasal challenge (80 µL) with dead Pseudomonas aeruginosa (1.0 x 10(12) CFU/mL). Bronchoalveolar lavage was performed 2 days later, with total cell count and differential cell analysis. The study group (n = 4) received clarithromycin treatment (50 mg/kg/day, intraperitoneal) for 5 days. Treatment was initiated 2 days before intranasal challenge. RESULTS: There was no significant difference in total cell count between the groups (mean: 2.0 x 10(6) and 1.3 x 10(6), respectively). In both groups, there was a predominance of neutrophils. However, the study group had a higher percentage of lymphocytes in the bronchoalveolar lavage than the control group (median of 19% vs 2.5%, P = .029). CONCLUSION: Clarithromycin alters the cytological pattern of bronchoalveolar lavage of Swiss mice with neutrophil pulmonary inflammation, significantly increasing the percentage of lymphocytes.Faculdade de Medicina / Universidade de São Paulo - FM/USP2004-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0041-87812004000300002Revista do Hospital das Clínicas v.59 n.3 2004reponame:Revista do Hospital das Clínicasinstname:Universidade de São Paulo (USP)instacron:USP10.1590/S0041-87812004000300002info:eu-repo/semantics/openAccessPinto,Leonardo AraújoCamozzato,CamilaAvozani,MoniqueMachado,Denise CantarelliJones,Marcus HerbertStein,Renato TetelbomPitrez,Paulo Márcio Condessaeng2004-07-28T00:00:00Zoai:scielo:S0041-87812004000300002Revistahttp://www.scielo.br/rhcPUBhttps://old.scielo.br/oai/scielo-oai.php||revista.hc@hcnet.usp.br1678-99030041-8781opendoar:2004-07-28T00:00Revista do Hospital das Clínicas - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Effect of clarithromycin on the cell profile of bronchoalveolar lavage fluid in mice with neutrophil-predominant lung disease
title Effect of clarithromycin on the cell profile of bronchoalveolar lavage fluid in mice with neutrophil-predominant lung disease
spellingShingle Effect of clarithromycin on the cell profile of bronchoalveolar lavage fluid in mice with neutrophil-predominant lung disease
Pinto,Leonardo Araújo
Clarithromycin
Lung inflammation
Lung
Mice
Neutrophil
title_short Effect of clarithromycin on the cell profile of bronchoalveolar lavage fluid in mice with neutrophil-predominant lung disease
title_full Effect of clarithromycin on the cell profile of bronchoalveolar lavage fluid in mice with neutrophil-predominant lung disease
title_fullStr Effect of clarithromycin on the cell profile of bronchoalveolar lavage fluid in mice with neutrophil-predominant lung disease
title_full_unstemmed Effect of clarithromycin on the cell profile of bronchoalveolar lavage fluid in mice with neutrophil-predominant lung disease
title_sort Effect of clarithromycin on the cell profile of bronchoalveolar lavage fluid in mice with neutrophil-predominant lung disease
author Pinto,Leonardo Araújo
author_facet Pinto,Leonardo Araújo
Camozzato,Camila
Avozani,Monique
Machado,Denise Cantarelli
Jones,Marcus Herbert
Stein,Renato Tetelbom
Pitrez,Paulo Márcio Condessa
author_role author
author2 Camozzato,Camila
Avozani,Monique
Machado,Denise Cantarelli
Jones,Marcus Herbert
Stein,Renato Tetelbom
Pitrez,Paulo Márcio Condessa
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pinto,Leonardo Araújo
Camozzato,Camila
Avozani,Monique
Machado,Denise Cantarelli
Jones,Marcus Herbert
Stein,Renato Tetelbom
Pitrez,Paulo Márcio Condessa
dc.subject.por.fl_str_mv Clarithromycin
Lung inflammation
Lung
Mice
Neutrophil
topic Clarithromycin
Lung inflammation
Lung
Mice
Neutrophil
description OBJECTIVE: Macrolide antibiotics have anti-inflammatory properties in lung diseases. The aim of this study was to investigate the effect of clarithromycin in pulmonary cellular inflammatory response in mice. METHOD: Eight adult Swiss mice were studied. All animals received an intranasal challenge (80 µL) with dead Pseudomonas aeruginosa (1.0 x 10(12) CFU/mL). Bronchoalveolar lavage was performed 2 days later, with total cell count and differential cell analysis. The study group (n = 4) received clarithromycin treatment (50 mg/kg/day, intraperitoneal) for 5 days. Treatment was initiated 2 days before intranasal challenge. RESULTS: There was no significant difference in total cell count between the groups (mean: 2.0 x 10(6) and 1.3 x 10(6), respectively). In both groups, there was a predominance of neutrophils. However, the study group had a higher percentage of lymphocytes in the bronchoalveolar lavage than the control group (median of 19% vs 2.5%, P = .029). CONCLUSION: Clarithromycin alters the cytological pattern of bronchoalveolar lavage of Swiss mice with neutrophil pulmonary inflammation, significantly increasing the percentage of lymphocytes.
publishDate 2004
dc.date.none.fl_str_mv 2004-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0041-87812004000300002
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0041-87812004000300002
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0041-87812004000300002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Faculdade de Medicina / Universidade de São Paulo - FM/USP
publisher.none.fl_str_mv Faculdade de Medicina / Universidade de São Paulo - FM/USP
dc.source.none.fl_str_mv Revista do Hospital das Clínicas v.59 n.3 2004
reponame:Revista do Hospital das Clínicas
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Revista do Hospital das Clínicas
collection Revista do Hospital das Clínicas
repository.name.fl_str_mv Revista do Hospital das Clínicas - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||revista.hc@hcnet.usp.br
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