Helicobacter pylori genotyping from positive clotests in patients with duodenal ulcer

Detalhes bibliográficos
Autor(a) principal: Mattar,Rejane
Data de Publicação: 2000
Outros Autores: Laudanna,Antonio Atílio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Hospital das Clínicas
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0041-87812000000500001
Resumo: Even though the seroprevalence of H. pylori may be high in the normal population, a minority develops peptic ulcer. Colonization of the gastric mucosa by more pathogenic vacA strains of H. pylori seems to be associated with enhanced gastric inflammation and duodenal ulcer. H. pylori genotyping from positive CLOtests was developed to determine the vacA genotypes and cagA status in 40 duodenal ulcer patients and for routine use. The pathogenic s1b/ m1/ cagA genotype was the most frequently occurring strain (17/42.5%); only two (5%) patients presented the s2/ m2 genotype, the less virulent strain. Multiple strains were also detected in 17 (42.5%) patients. Multiple strains of H. pylori colonizing the human stomach have been underestimated, because genotyping has been performed from cultures of H. pylori. We concluded that genotyping of H. pylori from a positive CLOtest had the advantages of reducing the number of biopsies taken during endoscopy, eliminating the step of culturing H. pylori, and assuring the presence of H. pylori in the specimen being processed.
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spelling Helicobacter pylori genotyping from positive clotests in patients with duodenal ulcerHelicobacter pyloriGenotypeDuodenal ulcerCLOtestEven though the seroprevalence of H. pylori may be high in the normal population, a minority develops peptic ulcer. Colonization of the gastric mucosa by more pathogenic vacA strains of H. pylori seems to be associated with enhanced gastric inflammation and duodenal ulcer. H. pylori genotyping from positive CLOtests was developed to determine the vacA genotypes and cagA status in 40 duodenal ulcer patients and for routine use. The pathogenic s1b/ m1/ cagA genotype was the most frequently occurring strain (17/42.5%); only two (5%) patients presented the s2/ m2 genotype, the less virulent strain. Multiple strains were also detected in 17 (42.5%) patients. Multiple strains of H. pylori colonizing the human stomach have been underestimated, because genotyping has been performed from cultures of H. pylori. We concluded that genotyping of H. pylori from a positive CLOtest had the advantages of reducing the number of biopsies taken during endoscopy, eliminating the step of culturing H. pylori, and assuring the presence of H. pylori in the specimen being processed.Faculdade de Medicina / Universidade de São Paulo - FM/USP2000-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0041-87812000000500001Revista do Hospital das Clínicas v.55 n.5 2000reponame:Revista do Hospital das Clínicasinstname:Universidade de São Paulo (USP)instacron:USP10.1590/S0041-87812000000500001info:eu-repo/semantics/openAccessMattar,RejaneLaudanna,Antonio Atílioeng2001-01-10T00:00:00Zoai:scielo:S0041-87812000000500001Revistahttp://www.scielo.br/rhcPUBhttps://old.scielo.br/oai/scielo-oai.php||revista.hc@hcnet.usp.br1678-99030041-8781opendoar:2001-01-10T00:00Revista do Hospital das Clínicas - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Helicobacter pylori genotyping from positive clotests in patients with duodenal ulcer
title Helicobacter pylori genotyping from positive clotests in patients with duodenal ulcer
spellingShingle Helicobacter pylori genotyping from positive clotests in patients with duodenal ulcer
Mattar,Rejane
Helicobacter pylori
Genotype
Duodenal ulcer
CLOtest
title_short Helicobacter pylori genotyping from positive clotests in patients with duodenal ulcer
title_full Helicobacter pylori genotyping from positive clotests in patients with duodenal ulcer
title_fullStr Helicobacter pylori genotyping from positive clotests in patients with duodenal ulcer
title_full_unstemmed Helicobacter pylori genotyping from positive clotests in patients with duodenal ulcer
title_sort Helicobacter pylori genotyping from positive clotests in patients with duodenal ulcer
author Mattar,Rejane
author_facet Mattar,Rejane
Laudanna,Antonio Atílio
author_role author
author2 Laudanna,Antonio Atílio
author2_role author
dc.contributor.author.fl_str_mv Mattar,Rejane
Laudanna,Antonio Atílio
dc.subject.por.fl_str_mv Helicobacter pylori
Genotype
Duodenal ulcer
CLOtest
topic Helicobacter pylori
Genotype
Duodenal ulcer
CLOtest
description Even though the seroprevalence of H. pylori may be high in the normal population, a minority develops peptic ulcer. Colonization of the gastric mucosa by more pathogenic vacA strains of H. pylori seems to be associated with enhanced gastric inflammation and duodenal ulcer. H. pylori genotyping from positive CLOtests was developed to determine the vacA genotypes and cagA status in 40 duodenal ulcer patients and for routine use. The pathogenic s1b/ m1/ cagA genotype was the most frequently occurring strain (17/42.5%); only two (5%) patients presented the s2/ m2 genotype, the less virulent strain. Multiple strains were also detected in 17 (42.5%) patients. Multiple strains of H. pylori colonizing the human stomach have been underestimated, because genotyping has been performed from cultures of H. pylori. We concluded that genotyping of H. pylori from a positive CLOtest had the advantages of reducing the number of biopsies taken during endoscopy, eliminating the step of culturing H. pylori, and assuring the presence of H. pylori in the specimen being processed.
publishDate 2000
dc.date.none.fl_str_mv 2000-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0041-87812000000500001
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0041-87812000000500001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0041-87812000000500001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Faculdade de Medicina / Universidade de São Paulo - FM/USP
publisher.none.fl_str_mv Faculdade de Medicina / Universidade de São Paulo - FM/USP
dc.source.none.fl_str_mv Revista do Hospital das Clínicas v.55 n.5 2000
reponame:Revista do Hospital das Clínicas
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Revista do Hospital das Clínicas
collection Revista do Hospital das Clínicas
repository.name.fl_str_mv Revista do Hospital das Clínicas - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||revista.hc@hcnet.usp.br
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