Effects of the HeNe laser and of the application mode on healing burns in rats
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Fisioterapia e Pesquisa |
Texto Completo: | https://www.revistas.usp.br/fpusp/article/view/75780 |
Resumo: | The aim of this study was to verify the effect of therapeutic laser in treating burns in Wistar rats. Sixty-two male rats had a 4 cm² area trichotomized at the dorsal region, were submitted to burn with hot vegetable oil at 300ºC, and were then divided into three groups. Treatment begun on the first day following burn. Experimental group 1 was treated with helium-neon (HeNe) array laser at 4 J/cm² and experimental group 2 with punctual laser at the same intensity, on four different points previously marked. Control group didn’t receive any treatment. Histological samples were collected on the 3rd, 7th, 14th, and 21st days after burn, blades being then analysed. Results show that the group treated with array laser was fully healed after 14 days of treatment, presenting normal structure skin, while the other groups only showed improvement after 21 days. Neutrophile cells were noticed on the 14th day in the group treated with punctual laser, suggesting that a new inflammatory process had taken place, probably due to the use of an intensity too high for this mode of laser therapy. Results allow concluding that the HeNe laser at 4 J/cm² intensity speeds up the healing process of skin burns and that, if applied in the array mode, it is still more effective. |
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Effects of the HeNe laser and of the application mode on healing burns in ratsEfeitos do laser HeNe e do modo de aplicação no processo de cicatrização de queimaduras em ratosanálise histológicacicatrização de feridasqueimadurasterapia a laser de baixa intensidade.analysishistologicalburnslaser therapylow levelwound healing.The aim of this study was to verify the effect of therapeutic laser in treating burns in Wistar rats. Sixty-two male rats had a 4 cm² area trichotomized at the dorsal region, were submitted to burn with hot vegetable oil at 300ºC, and were then divided into three groups. Treatment begun on the first day following burn. Experimental group 1 was treated with helium-neon (HeNe) array laser at 4 J/cm² and experimental group 2 with punctual laser at the same intensity, on four different points previously marked. Control group didn’t receive any treatment. Histological samples were collected on the 3rd, 7th, 14th, and 21st days after burn, blades being then analysed. Results show that the group treated with array laser was fully healed after 14 days of treatment, presenting normal structure skin, while the other groups only showed improvement after 21 days. Neutrophile cells were noticed on the 14th day in the group treated with punctual laser, suggesting that a new inflammatory process had taken place, probably due to the use of an intensity too high for this mode of laser therapy. Results allow concluding that the HeNe laser at 4 J/cm² intensity speeds up the healing process of skin burns and that, if applied in the array mode, it is still more effective.Esta pesquisa visou verificar os efeitos do laser terapêutico no tratamento de queimaduras em ratos da raça Wistar. Sessenta e dois ratos machos foram tricotomizados na região dorsal, em uma área de 4 cm², submetidos à queimadura por óleo vegetal quente a 300ºC e então divididos em três grupos. A partir do dia seguinte à lesão iniciou-se o tratamento. O grupo experimental 1 foi tratado com laser de hélio-neônio (HeNe) no modo varredura a 4 J/cm² e o grupo experimental 2 com laser pontual na mesma intensidade, em quatro diferentes pontos previamente demarcados. Os animais do grupo controle não receberam tratamento algum. Foram realizadas coletas histológicas aos 3o, 7o, 14o e 21o dias após a lesão, analisando-se as lâminas. Os resultados mostraram que o grupo tratado com laser varredura obteve cura total da lesão após 14 dias de tratamento, apresentando a pele com estrutura normal, enquanto os demais grupos apresentaram melhora apenas após 21 dias. No grupo tratado com laser pontual percebeu-se a presença de neutrófilos no 14o dia, o que sugere uma nova inflamação, que pode ter sido causada pela utilização de intensidade muito alta para essa forma de laserterapia. Os resultados permitem concluir que o laser HeNe em uma intensidade de 4 J/cm² acelera o processo de cicatrização de queimaduras cutâneas e, quando aplicado no modo varredura, os resultados são ainda mais efetivos.Universidade de São Paulo. Faculdade de Medicina2007-08-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/fpusp/article/view/7578010.1590/fpusp.v14i2.75780Fisioterapia e Pesquisa; Vol. 14 No. 2 (2007); 6-13Fisioterapia e Pesquisa; Vol. 14 Núm. 2 (2007); 6-13Fisioterapia e Pesquisa; v. 14 n. 2 (2007); 6-132316-91171809-2950reponame:Fisioterapia e Pesquisainstname:Universidade de São Paulo (USP)instacron:USPporhttps://www.revistas.usp.br/fpusp/article/view/75780/79265Copyright (c) 2017 Fisioterapia e Pesquisainfo:eu-repo/semantics/openAccessMello, Pâmela BilligSampedro, Renan Maximiliano FernandesPiccinini, Aline Martinelli2014-05-07T22:36:27Zoai:revistas.usp.br:article/75780Revistahttp://www.revistas.usp.br/fpuspPUBhttps://www.revistas.usp.br/fpusp/oai||revfisio@usp.br2316-91171809-2950opendoar:2014-05-07T22:36:27Fisioterapia e Pesquisa - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Effects of the HeNe laser and of the application mode on healing burns in rats Efeitos do laser HeNe e do modo de aplicação no processo de cicatrização de queimaduras em ratos |
title |
Effects of the HeNe laser and of the application mode on healing burns in rats |
spellingShingle |
Effects of the HeNe laser and of the application mode on healing burns in rats Mello, Pâmela Billig análise histológica cicatrização de feridas queimaduras terapia a laser de baixa intensidade. analysis histological burns laser therapy low level wound healing. |
title_short |
Effects of the HeNe laser and of the application mode on healing burns in rats |
title_full |
Effects of the HeNe laser and of the application mode on healing burns in rats |
title_fullStr |
Effects of the HeNe laser and of the application mode on healing burns in rats |
title_full_unstemmed |
Effects of the HeNe laser and of the application mode on healing burns in rats |
title_sort |
Effects of the HeNe laser and of the application mode on healing burns in rats |
author |
Mello, Pâmela Billig |
author_facet |
Mello, Pâmela Billig Sampedro, Renan Maximiliano Fernandes Piccinini, Aline Martinelli |
author_role |
author |
author2 |
Sampedro, Renan Maximiliano Fernandes Piccinini, Aline Martinelli |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Mello, Pâmela Billig Sampedro, Renan Maximiliano Fernandes Piccinini, Aline Martinelli |
dc.subject.por.fl_str_mv |
análise histológica cicatrização de feridas queimaduras terapia a laser de baixa intensidade. analysis histological burns laser therapy low level wound healing. |
topic |
análise histológica cicatrização de feridas queimaduras terapia a laser de baixa intensidade. analysis histological burns laser therapy low level wound healing. |
description |
The aim of this study was to verify the effect of therapeutic laser in treating burns in Wistar rats. Sixty-two male rats had a 4 cm² area trichotomized at the dorsal region, were submitted to burn with hot vegetable oil at 300ºC, and were then divided into three groups. Treatment begun on the first day following burn. Experimental group 1 was treated with helium-neon (HeNe) array laser at 4 J/cm² and experimental group 2 with punctual laser at the same intensity, on four different points previously marked. Control group didn’t receive any treatment. Histological samples were collected on the 3rd, 7th, 14th, and 21st days after burn, blades being then analysed. Results show that the group treated with array laser was fully healed after 14 days of treatment, presenting normal structure skin, while the other groups only showed improvement after 21 days. Neutrophile cells were noticed on the 14th day in the group treated with punctual laser, suggesting that a new inflammatory process had taken place, probably due to the use of an intensity too high for this mode of laser therapy. Results allow concluding that the HeNe laser at 4 J/cm² intensity speeds up the healing process of skin burns and that, if applied in the array mode, it is still more effective. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-08-31 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/fpusp/article/view/75780 10.1590/fpusp.v14i2.75780 |
url |
https://www.revistas.usp.br/fpusp/article/view/75780 |
identifier_str_mv |
10.1590/fpusp.v14i2.75780 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/fpusp/article/view/75780/79265 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Fisioterapia e Pesquisa info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Fisioterapia e Pesquisa |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina |
dc.source.none.fl_str_mv |
Fisioterapia e Pesquisa; Vol. 14 No. 2 (2007); 6-13 Fisioterapia e Pesquisa; Vol. 14 Núm. 2 (2007); 6-13 Fisioterapia e Pesquisa; v. 14 n. 2 (2007); 6-13 2316-9117 1809-2950 reponame:Fisioterapia e Pesquisa instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Fisioterapia e Pesquisa |
collection |
Fisioterapia e Pesquisa |
repository.name.fl_str_mv |
Fisioterapia e Pesquisa - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||revfisio@usp.br |
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1787713734902284289 |