Laserterapia e microcorrente na cicatrização de queimadura em ratos: terapias associadas ou isoladas?
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Fisioterapia e Pesquisa |
Texto Completo: | https://www.revistas.usp.br/fpusp/article/view/78343 |
Resumo: | This study aimed to investigate if there are differences between the associated and isolated therapies from the laser and micro current on healing of burn wound healing in rats. A total of 40 male rats were randomly allocated into four groups: control group (CG), micro current group (MG), laser group (LG) and laser/micro current group (LMG), treated with associated laser and micro current. Thermal damage was done on the back of the animal and a total of ten days therapy was performed. After treatment samples were taken from the lesions to perform semi quantitative histopathological study using Hematoxylin Eosin and Masson Trichrome. The Kruskal-Wallis and Dunn's Test were used for statistical analyses. We observed a significant difference between groups for production of fibroblasts (p=0.0003), collagen (p=0.0153), neoangiogenesis (p=0.0031) and skin annexes (p=0.0004). In semi-quantitative histological analysis, the LMG showed lower values in presence of collagen, fibroblasts and number of skin appendages, only for neoangiogenesis, the associated therapy showed similar values to single modality therapy groups. Laser and microcurrent have beneficial effects on tissue healing. However, it is suggested that the association of these two therapies reduces the effectiveness of the treatment when compared to single mode treatment. |
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Laserterapia e microcorrente na cicatrização de queimadura em ratos: terapias associadas ou isoladas? Laserterapia y microcorriente en la cicatrización de quemaduras en ratas: ¿terapias asociadas o aisladas? Low-level laser therapy and micro current in burn wound healing in rats: associated or isolated therapy? This study aimed to investigate if there are differences between the associated and isolated therapies from the laser and micro current on healing of burn wound healing in rats. A total of 40 male rats were randomly allocated into four groups: control group (CG), micro current group (MG), laser group (LG) and laser/micro current group (LMG), treated with associated laser and micro current. Thermal damage was done on the back of the animal and a total of ten days therapy was performed. After treatment samples were taken from the lesions to perform semi quantitative histopathological study using Hematoxylin Eosin and Masson Trichrome. The Kruskal-Wallis and Dunn's Test were used for statistical analyses. We observed a significant difference between groups for production of fibroblasts (p=0.0003), collagen (p=0.0153), neoangiogenesis (p=0.0031) and skin annexes (p=0.0004). In semi-quantitative histological analysis, the LMG showed lower values in presence of collagen, fibroblasts and number of skin appendages, only for neoangiogenesis, the associated therapy showed similar values to single modality therapy groups. Laser and microcurrent have beneficial effects on tissue healing. However, it is suggested that the association of these two therapies reduces the effectiveness of the treatment when compared to single mode treatment. Este estudio tiene el objetivo de investigar si hay diferencias entre las terapias asociadas y aisladas del láser y microcorrientes en la reparación de lesión por quemadura en ratas. Un total de 40 animales fueron divididos aleatoriamente en cuatro grupos: grupo control (GC), grupo microcorriente (GM), grupo láser (GL) y grupo láser/microcorriente (GLM), tratados con láser asociado a microcorrientes. Después de inducidas las lesiones térmicas en el dorso del animal, fueron realizados en total diez días de tratamiento. Las muestras de tejido fueron recolectadas para el estudio histopatológico semicuantitativo usando Hematoxilina Eosina y Tricómico de Masson. Fueron utilizados los tests de Kruskal-Wallis y post-hoc de Dunn's. Hubo diferencia significativa entre los grupos para la producción de fibroblastos (p=0,0003), colágeno (p=0,0153), neoangiogénesis (p=0,0031) y anexos cutáneos (p=0,0004). En el análisis histológico semicuantitativo, el GLM presentó valores menores en los parámetros histológicos de presencia de colágeno, número de fibroblastos y anexos cutáneos (p Este estudo teve o objetivo de investigar se há diferenças entre as terapias associadas e isoladas do laser e microcorrentes no reparo de lesão por queimadura em ratos. Um total de 40 animais foi dividido aleatoriamente em quatro grupos: grupo controle (GC); grupo microcorrente (GM), grupo laser (GL) e grupo laser/microcorrente (GLM), tratados com laser associado a microcorrentes. Após lesões térmicas induzidas no dorso do animal, foi realizado um total de dez dias de tratamento. Amostras do tecido foram coletadas para estudo histopatológico semiquantitativo com Hematoxilina Eosina e Tricrômico de Masson. Foram utilizados os testes de Kruskal-Wallis e post-hoc de Dunn. Houve diferença significativa entre os grupos para a produção de fibroblastos (p=0,0003), colágeno (p=0,0153), neoangiogênese (p=0,0031) e anexos cutâneos (p=0,0004). Na análise histológica semiquantitativa, o GLM apresentou valores menores nos parâmetros histológicos de presença de colágeno, número de fibroblastos e anexos cutâneos (pUniversidade de São Paulo. Faculdade de Medicina2013-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://www.revistas.usp.br/fpusp/article/view/7834310.1590/S1809-29502013000100005Fisioterapia e Pesquisa; Vol. 20 No. 1 (2013); 24-30Fisioterapia e Pesquisa; Vol. 20 Núm. 1 (2013); 24-30Fisioterapia e Pesquisa; v. 20 n. 1 (2013); 24-302316-91171809-2950reponame:Fisioterapia e Pesquisainstname:Universidade de São Paulo (USP)instacron:USPporenghttps://www.revistas.usp.br/fpusp/article/view/78343/82354https://www.revistas.usp.br/fpusp/article/view/78343/82355Copyright (c) 2017 Fisioterapia e Pesquisainfo:eu-repo/semantics/openAccessFreitas, Rodrigo Pegado de AbreuBarcelos, Ana Paula Medeiros deNóbrega, Brenda Medeiros daMacedo, Aline BarbosaOliveira, Anderson Rodrigues deRamos, Ana Maria de OliveiraVieira, Wouber Hérickson de Brito2014-04-02T17:27:22Zoai:revistas.usp.br:article/78343Revistahttp://www.revistas.usp.br/fpuspPUBhttps://www.revistas.usp.br/fpusp/oai||revfisio@usp.br2316-91171809-2950opendoar:2014-04-02T17:27:22Fisioterapia e Pesquisa - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Laserterapia e microcorrente na cicatrização de queimadura em ratos: terapias associadas ou isoladas? Laserterapia y microcorriente en la cicatrización de quemaduras en ratas: ¿terapias asociadas o aisladas? Low-level laser therapy and micro current in burn wound healing in rats: associated or isolated therapy? |
title |
Laserterapia e microcorrente na cicatrização de queimadura em ratos: terapias associadas ou isoladas? |
spellingShingle |
Laserterapia e microcorrente na cicatrização de queimadura em ratos: terapias associadas ou isoladas? Freitas, Rodrigo Pegado de Abreu |
title_short |
Laserterapia e microcorrente na cicatrização de queimadura em ratos: terapias associadas ou isoladas? |
title_full |
Laserterapia e microcorrente na cicatrização de queimadura em ratos: terapias associadas ou isoladas? |
title_fullStr |
Laserterapia e microcorrente na cicatrização de queimadura em ratos: terapias associadas ou isoladas? |
title_full_unstemmed |
Laserterapia e microcorrente na cicatrização de queimadura em ratos: terapias associadas ou isoladas? |
title_sort |
Laserterapia e microcorrente na cicatrização de queimadura em ratos: terapias associadas ou isoladas? |
author |
Freitas, Rodrigo Pegado de Abreu |
author_facet |
Freitas, Rodrigo Pegado de Abreu Barcelos, Ana Paula Medeiros de Nóbrega, Brenda Medeiros da Macedo, Aline Barbosa Oliveira, Anderson Rodrigues de Ramos, Ana Maria de Oliveira Vieira, Wouber Hérickson de Brito |
author_role |
author |
author2 |
Barcelos, Ana Paula Medeiros de Nóbrega, Brenda Medeiros da Macedo, Aline Barbosa Oliveira, Anderson Rodrigues de Ramos, Ana Maria de Oliveira Vieira, Wouber Hérickson de Brito |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Freitas, Rodrigo Pegado de Abreu Barcelos, Ana Paula Medeiros de Nóbrega, Brenda Medeiros da Macedo, Aline Barbosa Oliveira, Anderson Rodrigues de Ramos, Ana Maria de Oliveira Vieira, Wouber Hérickson de Brito |
description |
This study aimed to investigate if there are differences between the associated and isolated therapies from the laser and micro current on healing of burn wound healing in rats. A total of 40 male rats were randomly allocated into four groups: control group (CG), micro current group (MG), laser group (LG) and laser/micro current group (LMG), treated with associated laser and micro current. Thermal damage was done on the back of the animal and a total of ten days therapy was performed. After treatment samples were taken from the lesions to perform semi quantitative histopathological study using Hematoxylin Eosin and Masson Trichrome. The Kruskal-Wallis and Dunn's Test were used for statistical analyses. We observed a significant difference between groups for production of fibroblasts (p=0.0003), collagen (p=0.0153), neoangiogenesis (p=0.0031) and skin annexes (p=0.0004). In semi-quantitative histological analysis, the LMG showed lower values in presence of collagen, fibroblasts and number of skin appendages, only for neoangiogenesis, the associated therapy showed similar values to single modality therapy groups. Laser and microcurrent have beneficial effects on tissue healing. However, it is suggested that the association of these two therapies reduces the effectiveness of the treatment when compared to single mode treatment. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/fpusp/article/view/78343 10.1590/S1809-29502013000100005 |
url |
https://www.revistas.usp.br/fpusp/article/view/78343 |
identifier_str_mv |
10.1590/S1809-29502013000100005 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/fpusp/article/view/78343/82354 https://www.revistas.usp.br/fpusp/article/view/78343/82355 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Fisioterapia e Pesquisa info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Fisioterapia e Pesquisa |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina |
dc.source.none.fl_str_mv |
Fisioterapia e Pesquisa; Vol. 20 No. 1 (2013); 24-30 Fisioterapia e Pesquisa; Vol. 20 Núm. 1 (2013); 24-30 Fisioterapia e Pesquisa; v. 20 n. 1 (2013); 24-30 2316-9117 1809-2950 reponame:Fisioterapia e Pesquisa instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Fisioterapia e Pesquisa |
collection |
Fisioterapia e Pesquisa |
repository.name.fl_str_mv |
Fisioterapia e Pesquisa - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||revfisio@usp.br |
_version_ |
1787713735878508544 |