An integrative approach to delimit species in the Eulaema cingulata and Eulaema pseudocingulata pair (Hymenoptera: Apidae) using morphometric and molecular evidence

Detalhes bibliográficos
Autor(a) principal: Andrade, Tamires de Oliveira
Data de Publicação: 2021
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Biblioteca Digital de Teses e Dissertações da USP
Texto Completo: https://www.teses.usp.br/teses/disponiveis/38/38131/tde-19042021-113907/
Resumo: The study of species delimitation has been a rich scientific field that makes use of different sources of data in order to identify independently evolving lineages that might be recognized as species. Males of the solitary orchid bees Eulaema cingulata (Fabricius, 1804) and Eulaema pseudocingulata Oliveira, 2006 are morphologically similar, although differ in the shape of the velvety area of the middle leg. These nominal species are relatively commonly recorded in bee diversity studies in Brazil; they have been, however, recognized by conflicting hypotheses of interspecific delimitation. Here we investigate the limits of both nominal species combining distinct methods: geometric morphometrics (GM), phylogenetics, COI-barcoding and pairwise genetic p-distance with a species delimitation method based on multispecies coalescent. We obtained data from 126 representative specimens of the entire geographical range in which the nominal species occur and assess species boundaries under the general lineage concept. We found substantial overlapping in the shape of forewings and heads of species in the GM analysis. The results of COI-barcoding analysis showed pair-wise genetic distances lower than 3% (within E. cingulata 1.3% and within E. pseudocingulata 0.7%, while between the two species 0.95%). The Bayesian phylogenetic reconstruction clustered both species in a strongly supported monophyletic group, however, these forms were not reconstructed as distinct clades. The sequencing of UCEs recovered 2.180 homologous loci that provided no sufficient variability to recognize both forms either as different species or as independent evolutionary units. Our results support the recognition of E. cingulata and E. pseudocingulata as members of the same evolutionary unit.
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spelling An integrative approach to delimit species in the Eulaema cingulata and Eulaema pseudocingulata pair (Hymenoptera: Apidae) using morphometric and molecular evidenceUma abordagem integrativa para delimitar espécies no par Eulaema cingulata e Eulaema pseudocingulata (Hymenoptera: Apidae) utilizando evidência morfométrica e molecularAbelhas das OrquídeasBarcodingBarcodingDelimitação de EspéciesElementos UltraconservadosEuglossiniEuglossiniFilogenômicaOrchid BeesPhylogenomicsSpecies DelimitationTaxonomiaTaxonomyUltraconserved ElementsThe study of species delimitation has been a rich scientific field that makes use of different sources of data in order to identify independently evolving lineages that might be recognized as species. Males of the solitary orchid bees Eulaema cingulata (Fabricius, 1804) and Eulaema pseudocingulata Oliveira, 2006 are morphologically similar, although differ in the shape of the velvety area of the middle leg. These nominal species are relatively commonly recorded in bee diversity studies in Brazil; they have been, however, recognized by conflicting hypotheses of interspecific delimitation. Here we investigate the limits of both nominal species combining distinct methods: geometric morphometrics (GM), phylogenetics, COI-barcoding and pairwise genetic p-distance with a species delimitation method based on multispecies coalescent. We obtained data from 126 representative specimens of the entire geographical range in which the nominal species occur and assess species boundaries under the general lineage concept. We found substantial overlapping in the shape of forewings and heads of species in the GM analysis. The results of COI-barcoding analysis showed pair-wise genetic distances lower than 3% (within E. cingulata 1.3% and within E. pseudocingulata 0.7%, while between the two species 0.95%). The Bayesian phylogenetic reconstruction clustered both species in a strongly supported monophyletic group, however, these forms were not reconstructed as distinct clades. The sequencing of UCEs recovered 2.180 homologous loci that provided no sufficient variability to recognize both forms either as different species or as independent evolutionary units. Our results support the recognition of E. cingulata and E. pseudocingulata as members of the same evolutionary unit.O estudo de delimitação de espécies tem sido um fértil campo da ciência, que faz uso de diferentes fontes de dados para identificar linhagens evolutivas independentes que devam ser reconhecidas como espécies. Os machos das espécies de abelhas solitárias das orquídeas Eulaema cingulata (Fabricius, 1804) e Eulaema pseudocingulata Oliveira, 2006 têm sido reconhecidas como entidades diferentes na literatura, são morfologicamente similares e se diferenciariam pela forma da região aveludada da perna média. Registros dessas espécies nominais são relativamente comuns em estudos de diversidade de abelhas no Brasil; no entanto, estes nomes têm sido reconhecidos a partir de hipóteses conflitantes de delimitação interespecífica. Aqui nós investigamos se existem limites entre ambas as espécies combinando métodos como Morfometria Geométrica (MG), filogenética, COI-barcoding e distância genética com um método de delimitação de espécies baseado em coalescência de múltiplas espécies. Obtivemos dados de 126 espécimes representativos de toda a distribuição geográfica em que as espécies nominais ocorrem e avaliamos os limites das espécies sob o conceito de linhagem geral. Encontramos sobreposições substanciais na forma das asas anteriores e das cabeças na análise de MG. Os resultados da análise do COI-barcoding mostraram distâncias genéticas inferiores a 3% (dentro de E. cingulata 1,3% e dentro de E. pseudocingulata 0,7%, enquanto entre as duas espécies 0,95%). A reconstrução filogenética bayesiana agrupou ambas as formas em um grupo monofilético fortemente suportado, apesar delas não terem sido reconstruídas, entretanto, como clados distintos. O sequenciamento de UCEs recuperou 2.180 loci homólogos que corroboram com análises anteriores e não forneceram variabilidade suficiente para reconhecer ambas as formas como espécies biológicas diferentes ou como unidades evolutivas independentes. Nossos resultados apoiam o reconhecimento de ambas as formas como membros de uma mesma unidade evolutiva.Biblioteca Digitais de Teses e Dissertações da USPBrandão, Carlos Roberto FerreiraRamos, Kelli dos SantosAndrade, Tamires de Oliveira2021-03-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttps://www.teses.usp.br/teses/disponiveis/38/38131/tde-19042021-113907/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPLiberar o conteúdo para acesso público.info:eu-repo/semantics/openAccesseng2024-10-09T13:16:04Zoai:teses.usp.br:tde-19042021-113907Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212024-10-09T13:16:04Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv An integrative approach to delimit species in the Eulaema cingulata and Eulaema pseudocingulata pair (Hymenoptera: Apidae) using morphometric and molecular evidence
Uma abordagem integrativa para delimitar espécies no par Eulaema cingulata e Eulaema pseudocingulata (Hymenoptera: Apidae) utilizando evidência morfométrica e molecular
title An integrative approach to delimit species in the Eulaema cingulata and Eulaema pseudocingulata pair (Hymenoptera: Apidae) using morphometric and molecular evidence
spellingShingle An integrative approach to delimit species in the Eulaema cingulata and Eulaema pseudocingulata pair (Hymenoptera: Apidae) using morphometric and molecular evidence
Andrade, Tamires de Oliveira
Abelhas das Orquídeas
Barcoding
Barcoding
Delimitação de Espécies
Elementos Ultraconservados
Euglossini
Euglossini
Filogenômica
Orchid Bees
Phylogenomics
Species Delimitation
Taxonomia
Taxonomy
Ultraconserved Elements
title_short An integrative approach to delimit species in the Eulaema cingulata and Eulaema pseudocingulata pair (Hymenoptera: Apidae) using morphometric and molecular evidence
title_full An integrative approach to delimit species in the Eulaema cingulata and Eulaema pseudocingulata pair (Hymenoptera: Apidae) using morphometric and molecular evidence
title_fullStr An integrative approach to delimit species in the Eulaema cingulata and Eulaema pseudocingulata pair (Hymenoptera: Apidae) using morphometric and molecular evidence
title_full_unstemmed An integrative approach to delimit species in the Eulaema cingulata and Eulaema pseudocingulata pair (Hymenoptera: Apidae) using morphometric and molecular evidence
title_sort An integrative approach to delimit species in the Eulaema cingulata and Eulaema pseudocingulata pair (Hymenoptera: Apidae) using morphometric and molecular evidence
author Andrade, Tamires de Oliveira
author_facet Andrade, Tamires de Oliveira
author_role author
dc.contributor.none.fl_str_mv Brandão, Carlos Roberto Ferreira
Ramos, Kelli dos Santos
dc.contributor.author.fl_str_mv Andrade, Tamires de Oliveira
dc.subject.por.fl_str_mv Abelhas das Orquídeas
Barcoding
Barcoding
Delimitação de Espécies
Elementos Ultraconservados
Euglossini
Euglossini
Filogenômica
Orchid Bees
Phylogenomics
Species Delimitation
Taxonomia
Taxonomy
Ultraconserved Elements
topic Abelhas das Orquídeas
Barcoding
Barcoding
Delimitação de Espécies
Elementos Ultraconservados
Euglossini
Euglossini
Filogenômica
Orchid Bees
Phylogenomics
Species Delimitation
Taxonomia
Taxonomy
Ultraconserved Elements
description The study of species delimitation has been a rich scientific field that makes use of different sources of data in order to identify independently evolving lineages that might be recognized as species. Males of the solitary orchid bees Eulaema cingulata (Fabricius, 1804) and Eulaema pseudocingulata Oliveira, 2006 are morphologically similar, although differ in the shape of the velvety area of the middle leg. These nominal species are relatively commonly recorded in bee diversity studies in Brazil; they have been, however, recognized by conflicting hypotheses of interspecific delimitation. Here we investigate the limits of both nominal species combining distinct methods: geometric morphometrics (GM), phylogenetics, COI-barcoding and pairwise genetic p-distance with a species delimitation method based on multispecies coalescent. We obtained data from 126 representative specimens of the entire geographical range in which the nominal species occur and assess species boundaries under the general lineage concept. We found substantial overlapping in the shape of forewings and heads of species in the GM analysis. The results of COI-barcoding analysis showed pair-wise genetic distances lower than 3% (within E. cingulata 1.3% and within E. pseudocingulata 0.7%, while between the two species 0.95%). The Bayesian phylogenetic reconstruction clustered both species in a strongly supported monophyletic group, however, these forms were not reconstructed as distinct clades. The sequencing of UCEs recovered 2.180 homologous loci that provided no sufficient variability to recognize both forms either as different species or as independent evolutionary units. Our results support the recognition of E. cingulata and E. pseudocingulata as members of the same evolutionary unit.
publishDate 2021
dc.date.none.fl_str_mv 2021-03-17
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.teses.usp.br/teses/disponiveis/38/38131/tde-19042021-113907/
url https://www.teses.usp.br/teses/disponiveis/38/38131/tde-19042021-113907/
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv
dc.rights.driver.fl_str_mv Liberar o conteúdo para acesso público.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Liberar o conteúdo para acesso público.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
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dc.publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
dc.source.none.fl_str_mv
reponame:Biblioteca Digital de Teses e Dissertações da USP
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Biblioteca Digital de Teses e Dissertações da USP
collection Biblioteca Digital de Teses e Dissertações da USP
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)
repository.mail.fl_str_mv virginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.br
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