Temporal changes in the expression of genes involved in carbon partitioning in apical culms of contrasting sugarcane genotypes

Detalhes bibliográficos
Autor(a) principal: Hosaka, Guilherme Kenichi
Data de Publicação: 2021
Tipo de documento: Tese
Idioma: eng
Título da fonte: Biblioteca Digital de Teses e Dissertações da USP
Texto Completo: https://www.teses.usp.br/teses/disponiveis/105/105131/tde-26032021-104353/
Resumo: Multiple genes in sugarcane control sucrose accumulation and the biosynthesis of cell wall components; however, it is unclear how these genes are expressed in its apical culms. To better understand this process, we sequenced mRNA from +1 stem internodes collected from four genotypes with different concentrations of soluble solids. Culms were collected at four different time points, ranging from six to 12-month-old plants. Here we show differentially expressed genes related to sucrose metabolism and cell wall biosynthesis, including genes encoding invertases, sucrose synthase and cellulose synthase. Our results showed increased expression of invertases in IN84-58, the genotype with lower sugar and higher fiber content, as well as delayed expression of secondary cell wall-related cellulose synthase for the other genotypes. Interestingly, genes involved with hormone metabolism were differentially expressed across time points in the three genotypes with higher soluble solids content. A similar result was observed for genes controlling maturation and transition to reproductive stages, possibly a result of selection against flowering in sugarcane breeding programs. These results indicate that carbon partitioning in apical culms of contrasting genotypes is mainly associated with differential cell wall biosynthesis, and may include early modifications for subsequent sucrose accumulation. Co-expression network analysis identified transcription factors related to growth and development, showing a probable time shift for carbon partitioning occurred in 10-month-old plants.
id USP_44a3eb2563fb9781ed85d499832a6c44
oai_identifier_str oai:teses.usp.br:tde-26032021-104353
network_acronym_str USP
network_name_str Biblioteca Digital de Teses e Dissertações da USP
repository_id_str 2721
spelling Temporal changes in the expression of genes involved in carbon partitioning in apical culms of contrasting sugarcane genotypesAlterações temporais na expressão dos genes envolvidos na partição do carbono em colmos apicais de genótipos contrastantes de cana-de-açúcar SaccharumSaccharumCo-expression networkDifferential expressionExpressão diferencialRede de co-expressãoRNA-SeqRNA-SeqSólidos solúveisSoluble solidsMultiple genes in sugarcane control sucrose accumulation and the biosynthesis of cell wall components; however, it is unclear how these genes are expressed in its apical culms. To better understand this process, we sequenced mRNA from +1 stem internodes collected from four genotypes with different concentrations of soluble solids. Culms were collected at four different time points, ranging from six to 12-month-old plants. Here we show differentially expressed genes related to sucrose metabolism and cell wall biosynthesis, including genes encoding invertases, sucrose synthase and cellulose synthase. Our results showed increased expression of invertases in IN84-58, the genotype with lower sugar and higher fiber content, as well as delayed expression of secondary cell wall-related cellulose synthase for the other genotypes. Interestingly, genes involved with hormone metabolism were differentially expressed across time points in the three genotypes with higher soluble solids content. A similar result was observed for genes controlling maturation and transition to reproductive stages, possibly a result of selection against flowering in sugarcane breeding programs. These results indicate that carbon partitioning in apical culms of contrasting genotypes is mainly associated with differential cell wall biosynthesis, and may include early modifications for subsequent sucrose accumulation. Co-expression network analysis identified transcription factors related to growth and development, showing a probable time shift for carbon partitioning occurred in 10-month-old plants.Múltiplos genes na cana-de-açúcar controlam o acúmulo de sacarose e a biossíntese dos componentes da parede celular; contudo, não está claro como estes genes são expressos nos caules apicais. Para melhor compreender este processo, sequenciamos o mRNA do entrenó +1 de colmos coletados de quatro genótipos com diferentes concentrações de sólidos solúveis. Os colmos foram coletados em quatro tempos diferentes, variando de plantas com seis a 12 meses de idade. Neste trabalho, apresentamos genes diferentemente expressos relacionados ao metabolismo da sacarose e à biossíntese da parede celular, incluindo genes que codificam invertases, sacarose sintase e celulose sintase. Nos nossos resultados mostraram um aumento da expressão de invertases na IN84-58, genótipo com menor teor de açúcar e maior teor de fibras, bem como a expressão tardia da celulose synthase relacionada com a parede celular secundária para os outros genótipos. Curiosamente, os genes envolvidos no metabolismo de hormônios foram diferencialmente expressos ao longo do tempo nos três genótipos com maior conteúdo de sólidos solúveis. Um resultado semelhante foi observado para genes que controlam a maturação e a transição para as fases reprodutivas, possivelmente um resultado da seleção contra a floração em programas de melhoramento de cana-de-açúcar. Estes resultados indicam que a partição de carbono em caules apicais de genótipos contrastantes está principalmente associada na alteração da biossíntese de parede celular, podendo incluir modificações precoces para posterior acúmulo de sacarose. A análise da rede de coexpressão identificou fatores de transcrição relacionados com o crescimento e desenvolvimento, mostrando uma provável mudança de tempo para a partição de carbono ocorrida em plantas com 10 meses de idade.Biblioteca Digitais de Teses e Dissertações da USPMargarido, Gabriel Rodrigues AlvesHosaka, Guilherme Kenichi2021-02-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttps://www.teses.usp.br/teses/disponiveis/105/105131/tde-26032021-104353/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPLiberar o conteúdo para acesso público.info:eu-repo/semantics/openAccesseng2024-08-01T15:12:02Zoai:teses.usp.br:tde-26032021-104353Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212024-08-01T15:12:02Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Temporal changes in the expression of genes involved in carbon partitioning in apical culms of contrasting sugarcane genotypes
Alterações temporais na expressão dos genes envolvidos na partição do carbono em colmos apicais de genótipos contrastantes de cana-de-açúcar
title Temporal changes in the expression of genes involved in carbon partitioning in apical culms of contrasting sugarcane genotypes
spellingShingle Temporal changes in the expression of genes involved in carbon partitioning in apical culms of contrasting sugarcane genotypes
Hosaka, Guilherme Kenichi
Saccharum
Saccharum
Co-expression network
Differential expression
Expressão diferencial
Rede de co-expressão
RNA-Seq
RNA-Seq
Sólidos solúveis
Soluble solids
title_short Temporal changes in the expression of genes involved in carbon partitioning in apical culms of contrasting sugarcane genotypes
title_full Temporal changes in the expression of genes involved in carbon partitioning in apical culms of contrasting sugarcane genotypes
title_fullStr Temporal changes in the expression of genes involved in carbon partitioning in apical culms of contrasting sugarcane genotypes
title_full_unstemmed Temporal changes in the expression of genes involved in carbon partitioning in apical culms of contrasting sugarcane genotypes
title_sort Temporal changes in the expression of genes involved in carbon partitioning in apical culms of contrasting sugarcane genotypes
author Hosaka, Guilherme Kenichi
author_facet Hosaka, Guilherme Kenichi
author_role author
dc.contributor.none.fl_str_mv Margarido, Gabriel Rodrigues Alves
dc.contributor.author.fl_str_mv Hosaka, Guilherme Kenichi
dc.subject.por.fl_str_mv Saccharum
Saccharum
Co-expression network
Differential expression
Expressão diferencial
Rede de co-expressão
RNA-Seq
RNA-Seq
Sólidos solúveis
Soluble solids
topic Saccharum
Saccharum
Co-expression network
Differential expression
Expressão diferencial
Rede de co-expressão
RNA-Seq
RNA-Seq
Sólidos solúveis
Soluble solids
description Multiple genes in sugarcane control sucrose accumulation and the biosynthesis of cell wall components; however, it is unclear how these genes are expressed in its apical culms. To better understand this process, we sequenced mRNA from +1 stem internodes collected from four genotypes with different concentrations of soluble solids. Culms were collected at four different time points, ranging from six to 12-month-old plants. Here we show differentially expressed genes related to sucrose metabolism and cell wall biosynthesis, including genes encoding invertases, sucrose synthase and cellulose synthase. Our results showed increased expression of invertases in IN84-58, the genotype with lower sugar and higher fiber content, as well as delayed expression of secondary cell wall-related cellulose synthase for the other genotypes. Interestingly, genes involved with hormone metabolism were differentially expressed across time points in the three genotypes with higher soluble solids content. A similar result was observed for genes controlling maturation and transition to reproductive stages, possibly a result of selection against flowering in sugarcane breeding programs. These results indicate that carbon partitioning in apical culms of contrasting genotypes is mainly associated with differential cell wall biosynthesis, and may include early modifications for subsequent sucrose accumulation. Co-expression network analysis identified transcription factors related to growth and development, showing a probable time shift for carbon partitioning occurred in 10-month-old plants.
publishDate 2021
dc.date.none.fl_str_mv 2021-02-22
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.teses.usp.br/teses/disponiveis/105/105131/tde-26032021-104353/
url https://www.teses.usp.br/teses/disponiveis/105/105131/tde-26032021-104353/
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv
dc.rights.driver.fl_str_mv Liberar o conteúdo para acesso público.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Liberar o conteúdo para acesso público.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv
dc.publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
dc.source.none.fl_str_mv
reponame:Biblioteca Digital de Teses e Dissertações da USP
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Biblioteca Digital de Teses e Dissertações da USP
collection Biblioteca Digital de Teses e Dissertações da USP
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)
repository.mail.fl_str_mv virginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.br
_version_ 1815257354002759680