Cytotoxicity of Qualea grandiflora Mart. leaf extract (Vochysiaceae) on pre-osteoblast culture. Evaluation of cell viability and expression/activity of MMPs

Detalhes bibliográficos
Autor(a) principal: Tokuhara, Cintia Kazuko
Data de Publicação: 2016
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Biblioteca Digital de Teses e Dissertações da USP
Texto Completo: https://www.teses.usp.br/teses/disponiveis/25/25149/tde-16112021-154312/
Resumo: The use of medicinal plants with the purpose of treatment of many diseases is common. However, a scientific study to ensure the doses considered safe for consumption is required. Qualea grandiflora has been used in folk medicine to treat bloody diarrhea and nervous system depressant, antioxidant and anti-inflammatory activity and among other applications. The aim of this study was to investigate the influence of hydroalcoholic extract obtained from the leaves of Q. grandiflora on MC3T3-E1 pre-osteoblast lineage, correlating the effects of this plant on cell viability and expression and activity of matrix metalloproteinase. It was performed using serial dilution (1000 g/mL, 100 g/mL, 10.0 g/mL, 1.0 g/mL and 0.1 g/mL) of different concentrations of the hydroalcoholic extract of leaves Qualea exposed on preosteoblast MC3T3-E1 cell line. Four periods (24, 48, 72 and 96 h) were stipulated in order to assess cytotoxicity of the plant. From these safe concentrations were chosen (10.0 g/mL, 1.0 g/mL and 0.1 g/mL) and evaluated the expression and activity of MMPs of these cells exposed to the extract Qualea. We found in 70% EtOH extract of the leaves of Q. grandiflora, is mostly derived from gallic acid compounds and other compounds of flavonoid. From the concentrations evaluated, only the lower concentrations (0.1 g/mL, 1.0 g/mL and 10.0 g/mL) showed not be cytotoxic to this cell line and for concentrations higher than 10.0 g/mL caused decreased cell viability. An increase in MMP-9 expression in the group with the extract was found, though the activity was not confirmed by zymography assay. In conclusion, our results indicate what is already practiced by folk medicine as low extract concentrations shown to be safe for this cell line, but high concentrations showed cytotoxicity.
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spelling Cytotoxicity of Qualea grandiflora Mart. leaf extract (Vochysiaceae) on pre-osteoblast culture. Evaluation of cell viability and expression/activity of MMPsCitotoxicidade do extrato de folhas da Qualea grandiflora Mart. (Vochysiaceae) em cultura de pré-osteoblastos. Avaliação da viabilidade celular e expressão/atividade de MMPsCitotoxicidadeCitotoxicityMatrix metalloproteinaseMetaloproteinase de matrizQualea grandifloraQualea grandifloraViabilidadeViabilityThe use of medicinal plants with the purpose of treatment of many diseases is common. However, a scientific study to ensure the doses considered safe for consumption is required. Qualea grandiflora has been used in folk medicine to treat bloody diarrhea and nervous system depressant, antioxidant and anti-inflammatory activity and among other applications. The aim of this study was to investigate the influence of hydroalcoholic extract obtained from the leaves of Q. grandiflora on MC3T3-E1 pre-osteoblast lineage, correlating the effects of this plant on cell viability and expression and activity of matrix metalloproteinase. It was performed using serial dilution (1000 g/mL, 100 g/mL, 10.0 g/mL, 1.0 g/mL and 0.1 g/mL) of different concentrations of the hydroalcoholic extract of leaves Qualea exposed on preosteoblast MC3T3-E1 cell line. Four periods (24, 48, 72 and 96 h) were stipulated in order to assess cytotoxicity of the plant. From these safe concentrations were chosen (10.0 g/mL, 1.0 g/mL and 0.1 g/mL) and evaluated the expression and activity of MMPs of these cells exposed to the extract Qualea. We found in 70% EtOH extract of the leaves of Q. grandiflora, is mostly derived from gallic acid compounds and other compounds of flavonoid. From the concentrations evaluated, only the lower concentrations (0.1 g/mL, 1.0 g/mL and 10.0 g/mL) showed not be cytotoxic to this cell line and for concentrations higher than 10.0 g/mL caused decreased cell viability. An increase in MMP-9 expression in the group with the extract was found, though the activity was not confirmed by zymography assay. In conclusion, our results indicate what is already practiced by folk medicine as low extract concentrations shown to be safe for this cell line, but high concentrations showed cytotoxicity.O uso de plantas medicinais com o propósito de tratamento de inúmeras doenças é algo comum. No entanto, estudos científicos para garantir as doses consideradas seguras para consumo são necessários. A Qualea grandiflora tem sido usada na medicina popular para o tratamento de diarréia com sangue, além de depressor do sistema nervoso, atividade antioxidante e antiinflamatório entre outras aplicações. Assim, o objetivo deste estudo foi investigar a influência do extrato hidroalcoólico obtido das folhas de Q. grandiflora na linhagem MC3T3-E1, pré-osteoblastos, correlacionando os efeitos desta planta sobre a viabilidade celular, expressão e atividade de metaloproteinases da matriz. Foi realizada diluições seriadas (1000 g/mL, 100 g/mL, 10.0 g/mL, 1.0 g/mL e 0.1 g/mL) de diferentes concentrações do extrato hidroalcoólico de folhas Qualea e deixadas em contato com os préosteoblastos. Quatro períodos (24, 48, 72 e 96 h) foram avaliados de modo a verificar a citotoxicidade da planta. A partir das concentrações seguras escolhidas (10.0 g/mL, 1.0 g/mL e 0.1 g/mL) foram avaliados a expressão e atividade de MMPs destas células expostas ao extrato da Qualea. Encontramos no extrato das folhas de Q. grandiflora, principalmente derivados de compostos de ácido gálico e outros compostos de flavonóides. A partir das concentrações testadas, apenas as concentrações mais baixas (0,1 g/mL, 1.0 g/mL e 10.0 g/mL) não mostraram ser citotóxicas para esta linhagem celular e concentrações superiores a 10.0 g/mL, causaram uma diminuição na viabilidade celular. Um aumento da expressão de MMP-9 no grupo com o extrato foi encontrado, embora a atividade não tenha sido confirmada por ensaio de zimografia. Em conclusão, nossos resultados indicam o que já é praticado pela medicina popular, pois baixas concentrações de extrato mostraram ser seguras para esta linhagem celular, mas concentrações elevadas mostraram citotoxicidade.Biblioteca Digitais de Teses e Dissertações da USPOliveira, Rodrigo Cardoso deTokuhara, Cintia Kazuko2016-11-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttps://www.teses.usp.br/teses/disponiveis/25/25149/tde-16112021-154312/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPLiberar o conteúdo para acesso público.info:eu-repo/semantics/openAccesseng2024-08-15T19:41:02Zoai:teses.usp.br:tde-16112021-154312Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212024-08-15T19:41:02Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Cytotoxicity of Qualea grandiflora Mart. leaf extract (Vochysiaceae) on pre-osteoblast culture. Evaluation of cell viability and expression/activity of MMPs
Citotoxicidade do extrato de folhas da Qualea grandiflora Mart. (Vochysiaceae) em cultura de pré-osteoblastos. Avaliação da viabilidade celular e expressão/atividade de MMPs
title Cytotoxicity of Qualea grandiflora Mart. leaf extract (Vochysiaceae) on pre-osteoblast culture. Evaluation of cell viability and expression/activity of MMPs
spellingShingle Cytotoxicity of Qualea grandiflora Mart. leaf extract (Vochysiaceae) on pre-osteoblast culture. Evaluation of cell viability and expression/activity of MMPs
Tokuhara, Cintia Kazuko
Citotoxicidade
Citotoxicity
Matrix metalloproteinase
Metaloproteinase de matriz
Qualea grandiflora
Qualea grandiflora
Viabilidade
Viability
title_short Cytotoxicity of Qualea grandiflora Mart. leaf extract (Vochysiaceae) on pre-osteoblast culture. Evaluation of cell viability and expression/activity of MMPs
title_full Cytotoxicity of Qualea grandiflora Mart. leaf extract (Vochysiaceae) on pre-osteoblast culture. Evaluation of cell viability and expression/activity of MMPs
title_fullStr Cytotoxicity of Qualea grandiflora Mart. leaf extract (Vochysiaceae) on pre-osteoblast culture. Evaluation of cell viability and expression/activity of MMPs
title_full_unstemmed Cytotoxicity of Qualea grandiflora Mart. leaf extract (Vochysiaceae) on pre-osteoblast culture. Evaluation of cell viability and expression/activity of MMPs
title_sort Cytotoxicity of Qualea grandiflora Mart. leaf extract (Vochysiaceae) on pre-osteoblast culture. Evaluation of cell viability and expression/activity of MMPs
author Tokuhara, Cintia Kazuko
author_facet Tokuhara, Cintia Kazuko
author_role author
dc.contributor.none.fl_str_mv Oliveira, Rodrigo Cardoso de
dc.contributor.author.fl_str_mv Tokuhara, Cintia Kazuko
dc.subject.por.fl_str_mv Citotoxicidade
Citotoxicity
Matrix metalloproteinase
Metaloproteinase de matriz
Qualea grandiflora
Qualea grandiflora
Viabilidade
Viability
topic Citotoxicidade
Citotoxicity
Matrix metalloproteinase
Metaloproteinase de matriz
Qualea grandiflora
Qualea grandiflora
Viabilidade
Viability
description The use of medicinal plants with the purpose of treatment of many diseases is common. However, a scientific study to ensure the doses considered safe for consumption is required. Qualea grandiflora has been used in folk medicine to treat bloody diarrhea and nervous system depressant, antioxidant and anti-inflammatory activity and among other applications. The aim of this study was to investigate the influence of hydroalcoholic extract obtained from the leaves of Q. grandiflora on MC3T3-E1 pre-osteoblast lineage, correlating the effects of this plant on cell viability and expression and activity of matrix metalloproteinase. It was performed using serial dilution (1000 g/mL, 100 g/mL, 10.0 g/mL, 1.0 g/mL and 0.1 g/mL) of different concentrations of the hydroalcoholic extract of leaves Qualea exposed on preosteoblast MC3T3-E1 cell line. Four periods (24, 48, 72 and 96 h) were stipulated in order to assess cytotoxicity of the plant. From these safe concentrations were chosen (10.0 g/mL, 1.0 g/mL and 0.1 g/mL) and evaluated the expression and activity of MMPs of these cells exposed to the extract Qualea. We found in 70% EtOH extract of the leaves of Q. grandiflora, is mostly derived from gallic acid compounds and other compounds of flavonoid. From the concentrations evaluated, only the lower concentrations (0.1 g/mL, 1.0 g/mL and 10.0 g/mL) showed not be cytotoxic to this cell line and for concentrations higher than 10.0 g/mL caused decreased cell viability. An increase in MMP-9 expression in the group with the extract was found, though the activity was not confirmed by zymography assay. In conclusion, our results indicate what is already practiced by folk medicine as low extract concentrations shown to be safe for this cell line, but high concentrations showed cytotoxicity.
publishDate 2016
dc.date.none.fl_str_mv 2016-11-08
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language eng
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dc.rights.driver.fl_str_mv Liberar o conteúdo para acesso público.
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dc.publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
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