Anti-adherent and antifungal activity of synthetic and organic adhesive polymers on Candida albicans biofilms

• Autor(a) principal: Toala, Oscar Oswaldo Marcillo
• Data de Publicação: 2015
• Tipo de documento: Dissertação
• Idioma: eng
• Título da fonte: Biblioteca Digital de Teses e Dissertações da USP
• Texto Completo: https://www.teses.usp.br/teses/disponiveis/25/25146/tde-25102021-152415/
• topic: Adesivos; Adesivos Teciduais; Adhesives; Base de dentadura; Candida albicans; Candida albicans; Denture Bases; Tissue adhesives

The objective of this study was to analyze the effect of synthetic and organic adhesive on Candida albicans biofilms. Prevention and management of Candidaassociated denture stomatitis have been widely studied but still looking for an irrefutable mechanism. In the last years, the innovative application of Cyanoacrylates and Fibrin Sealant derived form Snake-venom with antimicrobial purposes has caught attention in medical fields. These coating materials could act directly on the applied surface causing cytopathic or cytotoxic effects on cells and microorganisms. Fibrin sealant and cyanoacrylates with different hydroxyl chains were coated on rectangular specimens (2-3m Ra) and inoculated with Candida albicans SC5314 (1.107 cells/mL) for 24 h. They were submitted to colony-forming units (CFU/mL) quantification assay, Colorimetric assay XTT and one specimen of each group was analyzed using Confocal Laser Scanning Microscopy for a qualitative analysis. The data was analyzed using Kruskal-Wallis and Dunn´s multiple comparisons tests (p<0.05). According to the CFU assay a growth reduction was observed in Octyl- Cyanoacrylate, Conventional Ethyl-Cyanoacrylate, Gel Ethyl-Cyanoacrylate and Butyl-Cyanoacrylate groups with 87, 84, 71 and 60 % respectively. The XTT assay demonstrated a decreased metabolic activity in Octyl-Cyanoacrylate (70%) and Conventional Ethyl-Cyanoacrylate (61%). The Confocal Images showed a reduced fungal colonization in all Cyanoacrylate groups with no sign of non-viable adhered cells on Octyl-Cyanoacrylate. Based on these findings, an anti-adherent effect against Candida albicans can be achieved by coating Octyl-Cyanoacrylate and Conventional Ethyl-Cyanoacrylate on heat-polymerized acrylic resin. Furthermore, more tests must be held on in order to understand the anti-fungal effect.