Generation and expression of circadian oscillation in the stingless bee Melipona quadrifasciata (Hymenoptera; Apinae; Meliponini)

Detalhes bibliográficos
Autor(a) principal: Yamashita, Cintia Etsuko
Data de Publicação: 2013
Tipo de documento: Tese
Idioma: eng
Título da fonte: Biblioteca Digital de Teses e Dissertações da USP
Texto Completo: http://www.teses.usp.br/teses/disponiveis/41/41135/tde-21032014-083209/
Resumo: Daily rhythms of insects are generated by a circadian system localized in the protocerebrum and in the optic lobes of the central nervous system. The circadian system is composed by coupled oscillators connected to input and output pathways. The oscillator generates rhythms by molecular processes, linked in feedback loops. In the input pathways the components are involved in light mediated-transduction. In the output, several neuropeptides are involved. Foragers of the stingless bee Melipona quadrifasciata exhibit a daily activity rhythm. Foragers have been used here to identify circadian components, through three different approaches: I) analysis of gene expression; II) identification of structures in the central nervous system; III) comparative study of neuropeptides possibly related with the circadian system, using Apis mellifera as the reference species. I) Fragments of putative clock genes were cloned. Only period (per) gene showed rhythmic expression, peaked at 1h after lights off. Non statistically significant rhythms were detected in cryptochrome (cry), clock and cycle genes expression. II) Antibodies against PER, CRY (an input pathway protein) and pigment dispersing hormone (an output pathway neuropeptide) evinced several areas in the brain and in the optic lobes. PER and CRY were localized in the optic lobes and in fibers in the protocerebral region, in a rhythmic pattern. PDH was observed in cell bodies in the lateral protocerebrum, in projections in the brain and in some fibers in the optic lobes. III) Neuropeptides probably related to the circadian system, were found in A. mellifera and M. quadrifasciata. Some of them: tachykinin-related peptide, allatostatin, and FMRF-related peptide were rhythmic and present in specie-specific patterns. The circadian system of M. quadrifasciata showed particularities in the putative clock components when compared with A. mellifera and other insects. The expression, localization, distribution and temporal dynamics of the circadian system point out a novel, specific feature
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spelling Generation and expression of circadian oscillation in the stingless bee Melipona quadrifasciata (Hymenoptera; Apinae; Meliponini)Geração e expressão da oscilação circadiana na abelha sem ferrão Melipona quadrifasciata (Hymenoptera; Apinae; Meliponini)Cicardian rhythmsClock genesGenes do relógio biológicoMelipona QuadrifasciadaMelipona QuadrifasciadaRitmo cicardianoDaily rhythms of insects are generated by a circadian system localized in the protocerebrum and in the optic lobes of the central nervous system. The circadian system is composed by coupled oscillators connected to input and output pathways. The oscillator generates rhythms by molecular processes, linked in feedback loops. In the input pathways the components are involved in light mediated-transduction. In the output, several neuropeptides are involved. Foragers of the stingless bee Melipona quadrifasciata exhibit a daily activity rhythm. Foragers have been used here to identify circadian components, through three different approaches: I) analysis of gene expression; II) identification of structures in the central nervous system; III) comparative study of neuropeptides possibly related with the circadian system, using Apis mellifera as the reference species. I) Fragments of putative clock genes were cloned. Only period (per) gene showed rhythmic expression, peaked at 1h after lights off. Non statistically significant rhythms were detected in cryptochrome (cry), clock and cycle genes expression. II) Antibodies against PER, CRY (an input pathway protein) and pigment dispersing hormone (an output pathway neuropeptide) evinced several areas in the brain and in the optic lobes. PER and CRY were localized in the optic lobes and in fibers in the protocerebral region, in a rhythmic pattern. PDH was observed in cell bodies in the lateral protocerebrum, in projections in the brain and in some fibers in the optic lobes. III) Neuropeptides probably related to the circadian system, were found in A. mellifera and M. quadrifasciata. Some of them: tachykinin-related peptide, allatostatin, and FMRF-related peptide were rhythmic and present in specie-specific patterns. The circadian system of M. quadrifasciata showed particularities in the putative clock components when compared with A. mellifera and other insects. The expression, localization, distribution and temporal dynamics of the circadian system point out a novel, specific featureRitmos diários em insetos são gerados por um sistema circadiano localizado no protocerebrum e nos lobos ópticos do sistema nervoso central. O sistema circadiano é composto por osciladores acoplados às vias de aferência e eferência. O oscilador gera ritmos através de mecanismos moleculares, integrantes de alças de retroalimentação. Nas vias de aferência estão envolvidos componentes que participam da transdução mediada da luz. Diversos neuropeptídeos fazem parte das vias de eferência. Forrageiras da abelha sem ferrão Melipona quadrifasciata exibem um ritmo diário de atividade. Forrageiras foram utilizadas neste trabalho para identificar componentes circadianos, através de três diferentes abordagens: I) análise da expressão gênica, II) identificação de estruturas no sistema nervoso central, III) estudo comparado de neuropeptídeos possivelmente relacionados com o sistema circadiano, utilizando como espécie referência Apis mellifera. Fragmentos de prováveis genes do relógio foram clonados. Somente o gene period (per) mostrou expressão rítmica, o pico ocorreu 1h após o início do escuro. cryptochrome (cry), clock e cycle não apresentaram diferença estatística na expressão rítmica. I) Anticorpos contra PER, CRY (proteína da via de aferência) e \"pigment dispersing hormone\" (PDH, neuropeptídeo da via de eferência) marcaram diversas áreas no cérebro e nos lobos ópticos. PER e CRY foram localizados nos lobos ópticos, em fibras da região protocerebral, com um padrão rítmico. PDH foi observado em corpos celulares no protocerebrum lateral, em projeções no cérebro e em algumas fibras nos lobos ópticos. II) Neuropeptídeos, provavelmente relacionados com o sistema circadiano, foram detectados em A. mellifera e M. quadrifasciata. Alguns deles: \"tachykinins-related peptides\", alatostatinas, e \"FMRF-related peptides\" são rítmicos, com padrões espécie-específicos. O sistema circadiano de M. quadrifasciata mostrou particularidades nos prováveis componentes do relógio quando comparados com A. mellifera e outros insetos. A expressão, localização, distribuição e dinâmicas temporais apontam para características específicas da organização do sistema circadianoBiblioteca Digitais de Teses e Dissertações da USPHartfelder, Klaus HartmannMarques, Mirian DavidYamashita, Cintia Etsuko2013-10-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttp://www.teses.usp.br/teses/disponiveis/41/41135/tde-21032014-083209/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPLiberar o conteúdo para acesso público.info:eu-repo/semantics/openAccesseng2019-03-20T06:00:33Zoai:teses.usp.br:tde-21032014-083209Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212019-03-20T06:00:33Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Generation and expression of circadian oscillation in the stingless bee Melipona quadrifasciata (Hymenoptera; Apinae; Meliponini)
Geração e expressão da oscilação circadiana na abelha sem ferrão Melipona quadrifasciata (Hymenoptera; Apinae; Meliponini)
title Generation and expression of circadian oscillation in the stingless bee Melipona quadrifasciata (Hymenoptera; Apinae; Meliponini)
spellingShingle Generation and expression of circadian oscillation in the stingless bee Melipona quadrifasciata (Hymenoptera; Apinae; Meliponini)
Yamashita, Cintia Etsuko
Cicardian rhythms
Clock genes
Genes do relógio biológico
Melipona Quadrifasciada
Melipona Quadrifasciada
Ritmo cicardiano
title_short Generation and expression of circadian oscillation in the stingless bee Melipona quadrifasciata (Hymenoptera; Apinae; Meliponini)
title_full Generation and expression of circadian oscillation in the stingless bee Melipona quadrifasciata (Hymenoptera; Apinae; Meliponini)
title_fullStr Generation and expression of circadian oscillation in the stingless bee Melipona quadrifasciata (Hymenoptera; Apinae; Meliponini)
title_full_unstemmed Generation and expression of circadian oscillation in the stingless bee Melipona quadrifasciata (Hymenoptera; Apinae; Meliponini)
title_sort Generation and expression of circadian oscillation in the stingless bee Melipona quadrifasciata (Hymenoptera; Apinae; Meliponini)
author Yamashita, Cintia Etsuko
author_facet Yamashita, Cintia Etsuko
author_role author
dc.contributor.none.fl_str_mv Hartfelder, Klaus Hartmann
Marques, Mirian David
dc.contributor.author.fl_str_mv Yamashita, Cintia Etsuko
dc.subject.por.fl_str_mv Cicardian rhythms
Clock genes
Genes do relógio biológico
Melipona Quadrifasciada
Melipona Quadrifasciada
Ritmo cicardiano
topic Cicardian rhythms
Clock genes
Genes do relógio biológico
Melipona Quadrifasciada
Melipona Quadrifasciada
Ritmo cicardiano
description Daily rhythms of insects are generated by a circadian system localized in the protocerebrum and in the optic lobes of the central nervous system. The circadian system is composed by coupled oscillators connected to input and output pathways. The oscillator generates rhythms by molecular processes, linked in feedback loops. In the input pathways the components are involved in light mediated-transduction. In the output, several neuropeptides are involved. Foragers of the stingless bee Melipona quadrifasciata exhibit a daily activity rhythm. Foragers have been used here to identify circadian components, through three different approaches: I) analysis of gene expression; II) identification of structures in the central nervous system; III) comparative study of neuropeptides possibly related with the circadian system, using Apis mellifera as the reference species. I) Fragments of putative clock genes were cloned. Only period (per) gene showed rhythmic expression, peaked at 1h after lights off. Non statistically significant rhythms were detected in cryptochrome (cry), clock and cycle genes expression. II) Antibodies against PER, CRY (an input pathway protein) and pigment dispersing hormone (an output pathway neuropeptide) evinced several areas in the brain and in the optic lobes. PER and CRY were localized in the optic lobes and in fibers in the protocerebral region, in a rhythmic pattern. PDH was observed in cell bodies in the lateral protocerebrum, in projections in the brain and in some fibers in the optic lobes. III) Neuropeptides probably related to the circadian system, were found in A. mellifera and M. quadrifasciata. Some of them: tachykinin-related peptide, allatostatin, and FMRF-related peptide were rhythmic and present in specie-specific patterns. The circadian system of M. quadrifasciata showed particularities in the putative clock components when compared with A. mellifera and other insects. The expression, localization, distribution and temporal dynamics of the circadian system point out a novel, specific feature
publishDate 2013
dc.date.none.fl_str_mv 2013-10-25
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.teses.usp.br/teses/disponiveis/41/41135/tde-21032014-083209/
url http://www.teses.usp.br/teses/disponiveis/41/41135/tde-21032014-083209/
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv
dc.rights.driver.fl_str_mv Liberar o conteúdo para acesso público.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Liberar o conteúdo para acesso público.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
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dc.publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
dc.source.none.fl_str_mv
reponame:Biblioteca Digital de Teses e Dissertações da USP
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
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institution USP
reponame_str Biblioteca Digital de Teses e Dissertações da USP
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repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)
repository.mail.fl_str_mv virginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.br
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