Genetic analysis of ROS modulation in Sporisorium scitamineum - energy cane interaction

• Autor(a) principal: Ferreti, Joyce Dellavechia
• Data de Publicação: 2022
• Tipo de documento: Dissertação
• Idioma: eng
• Título da fonte: Biblioteca Digital de Teses e Dissertações da USP
• Texto Completo: https://www.teses.usp.br/teses/disponiveis/11/11135/tde-12072022-141606/
• topic: Antioxidant system; Breeding programs; Differentially expressed genes; Doença do carvão; Espécies reativas de oxigênio; Genes diferencialmente expressos; Melhoramento genético; Reactive oxygen species; Sistema antioxidante; Smut diseases

Energy cane is a crop developed for bioenergy production and shows a high economic potential for the country. Considering the energy cane increasing relevance, studies on the factors that may impact crop production are particularly important. Among loss production causes, the biotrophic fungus Sporisorium scitamineum causal agent of smut disease, is a concern in the development of new resistant varieties given the yield decrease caused by the disease. Therefore, several studies have been developed to improve the understanding of host defense mechanisms to improve genetic breeding programs and disease control. In this study, a genetic analysis was performed for host defense aspects related to reactive oxygen species (ROS) metabolism of susceptible (Vertix1) and resistant (Vertix2) energy cane genotypes in interaction with S. scitamineum. This dissertation is submitted in the format of 3 chapters, beginning with a review in chapter 1. In chapter 2, analyses from the two genotypes transcriptome were performed to further understand the genetic modulation involved in ROS metabolism at 48 hours post-inoculation with S. scitamineum. Differentially expressed genes (DEGs), related to ROS metabolism, were observed in common in both genotypes due the fungal presence, however showed contrasting expression patterns. Specific DEGs were also functionally analyzed for resistant and susceptible varieties against smut inoculation. In the third chapter, the results obtained through gene expression analysis of genes related to the antioxidant system, triggered by the oxidative stress response in the interaction at 48 hpi and 72 hpi, showed significant differences for TRX gene (in susceptible genotype) and SOD gene (in resistant genotype) only for analyses at 48 hpi. We observed in energy cane varieties a different pattern than already studied for conventional sugarcane in S. scitamineum interaction, involving these same ROS modulation genes. This knowledge is relevant for the new research development related to genetic breeding strategies for energy cane genotypes regarding smut disease resistance.