Characterization of the foliar uptake of zinc sources by soybean (Glycine max L.)

Detalhes bibliográficos
Autor(a) principal: Gomes, Marcos Henrique Feresin
Data de Publicação: 2021
Tipo de documento: Tese
Idioma: eng
Título da fonte: Biblioteca Digital de Teses e Dissertações da USP
Texto Completo: https://www.teses.usp.br/teses/disponiveis/64/64135/tde-31102023-093912/
Resumo: Zinc (Zn) is an essential element for plant, animal and human nutrition. Around the world, ca. 800,000 children under 5 years die annually due to a Zn-deficient diet. The application of zinc in crops can be performed on plant leaves or in the soil, however, the low use-efficiency is remarkable. This study aims to understand and characterize the absorption, transport and metabolization of Zn when applied on soybean leaves by the most common fertilizers used by worldwide, i.e. inorganic salts, complexes/chelates, and concentrated suspensions, as well as evaluate possibility of the employment of new technologies, such as nanoparticles and cellulose microspheres. X-ray fluorescence (XRF) and X-ray absorption (XANES) spectroscopy were employed to perform in vivo analysis on soybean plants together with greenhouse trials. The absorption and transport of Zn depended on the type of Zn source. Zinc from ZnSO4 was absorbed and transported faster than ZnO commercial suspension, while Zn applied as Zn-phosphite was transported faster than Zn-EDTA. The XANES analysis demonstrated that Zn from ZnSO4 and Zn phosphite was transported bound with organic acids such as malate and citrate. Conversely, Zn supplied by Zn-EDTA was transported in its pristine form. In a short-term experiment, i.e. few days, cellulose microspheres were able to reduce the toxicity caused by ZnSO4 salts and increase Zn transport trough leaf petiole. However, under longer evaluation, i.e. a couple of weeks, the root application of ZnSO4 increased by 42% the Zn accumulation in soybean plants compared to the control (low Zn supply). Additionally, root uptake mechanism under hydroponics was more efficient than the foliar application of ZnSO4, ZnO nanoparticles and ZnSO4 + cellulose microspheres. The foliar and root application of Zn did not affect the activity of ezymes related to the metabolism of reactive oxygen species. Understanding the mechanisms ruling the foliar absorption and metabolization of nutrients is fundamental for the development of the next generation of fertilizers. Therefore, more studies on this subject are necessary
id USP_73fac10948900d1f4269623a023cfe73
oai_identifier_str oai:teses.usp.br:tde-31102023-093912
network_acronym_str USP
network_name_str Biblioteca Digital de Teses e Dissertações da USP
repository_id_str 2721
spelling Characterization of the foliar uptake of zinc sources by soybean (Glycine max L.)Caracterização da absorção foliar de fontes de zinco pela cultura da soja (Glycine max L.)Adubação foliarCellulose microbeads. NanoparticlesEspectroscopia de Absorção de Raios X Próximo da Borda (XANES)Espectroscopia por Fluorescência de Raios X (XRF)Foliar fertilizationMicroesferas de celulose. NanopartículasX-Ray Absorption Near Edge Spectroscopy (XANES)X-Ray Fluorescence Spectroscopy (XRF)ZnZinc (Zn) is an essential element for plant, animal and human nutrition. Around the world, ca. 800,000 children under 5 years die annually due to a Zn-deficient diet. The application of zinc in crops can be performed on plant leaves or in the soil, however, the low use-efficiency is remarkable. This study aims to understand and characterize the absorption, transport and metabolization of Zn when applied on soybean leaves by the most common fertilizers used by worldwide, i.e. inorganic salts, complexes/chelates, and concentrated suspensions, as well as evaluate possibility of the employment of new technologies, such as nanoparticles and cellulose microspheres. X-ray fluorescence (XRF) and X-ray absorption (XANES) spectroscopy were employed to perform in vivo analysis on soybean plants together with greenhouse trials. The absorption and transport of Zn depended on the type of Zn source. Zinc from ZnSO4 was absorbed and transported faster than ZnO commercial suspension, while Zn applied as Zn-phosphite was transported faster than Zn-EDTA. The XANES analysis demonstrated that Zn from ZnSO4 and Zn phosphite was transported bound with organic acids such as malate and citrate. Conversely, Zn supplied by Zn-EDTA was transported in its pristine form. In a short-term experiment, i.e. few days, cellulose microspheres were able to reduce the toxicity caused by ZnSO4 salts and increase Zn transport trough leaf petiole. However, under longer evaluation, i.e. a couple of weeks, the root application of ZnSO4 increased by 42% the Zn accumulation in soybean plants compared to the control (low Zn supply). Additionally, root uptake mechanism under hydroponics was more efficient than the foliar application of ZnSO4, ZnO nanoparticles and ZnSO4 + cellulose microspheres. The foliar and root application of Zn did not affect the activity of ezymes related to the metabolism of reactive oxygen species. Understanding the mechanisms ruling the foliar absorption and metabolization of nutrients is fundamental for the development of the next generation of fertilizers. Therefore, more studies on this subject are necessaryO zinco (Zn) é um elemento essencial para a nutrição vegetal, animal e humana. Em todo o mundo, aproximadamente 800.000 crianças menores de 5 anos morrem anualmente devido a deficiência de Zn em suas dietas. A aplicação de Zn nas lavouras pode ser realizada nas folhas ou no solo, porém, é notável a baixa eficiência de sua utilização. Este estudo buscou caracterizar e entender a absorção, transporte e metabolização de Zn quando aplicado em folhas de soja pelas fontes de fertilizantes mais utilizadas mundialmente, i.e. sais inorgânicos, complexos/quelatos e suspensões concentradas, bem como avaliar a possibilidade de utilização de novas tecnologias, tais como nanopartículas e microesferas de celulose. As espectrometrias de fluorescência (XRF) e absorção de raios X (XANES) foram empregadas na realização de análises in vivo, juntamente com ensaios em casa de vegetação. A absorção e transporte de Zn foram dependentes da fonte aplicada. O Zn aplicado como ZnSO4 foi mais rapidamente absorvido e transportado do que aquele provindo de suspensão concentrada comercial, ao passo que o Zn aplicado como fosfito de Zn foi transportado mais rápido quando comparado ao Zn-EDTA. A ferramenta XANES demonstrou que o Zn aplicado como ZnSO4 e fosfito de Zn foi transportado ligado a ácidos orgânicos, como malato e citrato, por outro lado, o Zn advindo do Zn-EDTA permaneceu em sua forma primitiva. Em um curto período, i.e. alguns dias, as microesferas de celulose foram capazes de reduzir a toxicidade causada pelo ZnSO4 e aumentar o transporte de Zn no pecíolo das folhas. Contudo, em avaliações mais longas, i.e. na escala de semanas, a aplicação de ZnSO4 na raiz aumentou em 42% o acúmulo de Zn nas plantas de soja em relação ao controle (baixo suprimento de Zn). A capacidade de transferência de Zn pela via radicular, em solução nutritiva, também foi maior do que aquela oferecida pela aplicação foliar de ZnSO4, nanopartículas de ZnO e ZnSO4 + microesferas de celulose. A aplicação foliar e radicular de Zn não afetou o a atividade das enzimas do sistema antioxidante. Compreender os mecanismos que regem a absorção foliar e a metabolização de nutrientes é fundamental para o desenvolvimento da próxima geração de fertilizantes, portanto mais estudos nesta temática são necessáriosBiblioteca Digitais de Teses e Dissertações da USPCarvalho, Hudson Wallace Pereira deGomes, Marcos Henrique Feresin2021-10-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttps://www.teses.usp.br/teses/disponiveis/64/64135/tde-31102023-093912/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPLiberar o conteúdo para acesso público.info:eu-repo/semantics/openAccesseng2023-11-21T12:36:02Zoai:teses.usp.br:tde-31102023-093912Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212023-11-21T12:36:02Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Characterization of the foliar uptake of zinc sources by soybean (Glycine max L.)
Caracterização da absorção foliar de fontes de zinco pela cultura da soja (Glycine max L.)
title Characterization of the foliar uptake of zinc sources by soybean (Glycine max L.)
spellingShingle Characterization of the foliar uptake of zinc sources by soybean (Glycine max L.)
Gomes, Marcos Henrique Feresin
Adubação foliar
Cellulose microbeads. Nanoparticles
Espectroscopia de Absorção de Raios X Próximo da Borda (XANES)
Espectroscopia por Fluorescência de Raios X (XRF)
Foliar fertilization
Microesferas de celulose. Nanopartículas
X-Ray Absorption Near Edge Spectroscopy (XANES)
X-Ray Fluorescence Spectroscopy (XRF)
Zn
title_short Characterization of the foliar uptake of zinc sources by soybean (Glycine max L.)
title_full Characterization of the foliar uptake of zinc sources by soybean (Glycine max L.)
title_fullStr Characterization of the foliar uptake of zinc sources by soybean (Glycine max L.)
title_full_unstemmed Characterization of the foliar uptake of zinc sources by soybean (Glycine max L.)
title_sort Characterization of the foliar uptake of zinc sources by soybean (Glycine max L.)
author Gomes, Marcos Henrique Feresin
author_facet Gomes, Marcos Henrique Feresin
author_role author
dc.contributor.none.fl_str_mv Carvalho, Hudson Wallace Pereira de
dc.contributor.author.fl_str_mv Gomes, Marcos Henrique Feresin
dc.subject.por.fl_str_mv Adubação foliar
Cellulose microbeads. Nanoparticles
Espectroscopia de Absorção de Raios X Próximo da Borda (XANES)
Espectroscopia por Fluorescência de Raios X (XRF)
Foliar fertilization
Microesferas de celulose. Nanopartículas
X-Ray Absorption Near Edge Spectroscopy (XANES)
X-Ray Fluorescence Spectroscopy (XRF)
Zn
topic Adubação foliar
Cellulose microbeads. Nanoparticles
Espectroscopia de Absorção de Raios X Próximo da Borda (XANES)
Espectroscopia por Fluorescência de Raios X (XRF)
Foliar fertilization
Microesferas de celulose. Nanopartículas
X-Ray Absorption Near Edge Spectroscopy (XANES)
X-Ray Fluorescence Spectroscopy (XRF)
Zn
description Zinc (Zn) is an essential element for plant, animal and human nutrition. Around the world, ca. 800,000 children under 5 years die annually due to a Zn-deficient diet. The application of zinc in crops can be performed on plant leaves or in the soil, however, the low use-efficiency is remarkable. This study aims to understand and characterize the absorption, transport and metabolization of Zn when applied on soybean leaves by the most common fertilizers used by worldwide, i.e. inorganic salts, complexes/chelates, and concentrated suspensions, as well as evaluate possibility of the employment of new technologies, such as nanoparticles and cellulose microspheres. X-ray fluorescence (XRF) and X-ray absorption (XANES) spectroscopy were employed to perform in vivo analysis on soybean plants together with greenhouse trials. The absorption and transport of Zn depended on the type of Zn source. Zinc from ZnSO4 was absorbed and transported faster than ZnO commercial suspension, while Zn applied as Zn-phosphite was transported faster than Zn-EDTA. The XANES analysis demonstrated that Zn from ZnSO4 and Zn phosphite was transported bound with organic acids such as malate and citrate. Conversely, Zn supplied by Zn-EDTA was transported in its pristine form. In a short-term experiment, i.e. few days, cellulose microspheres were able to reduce the toxicity caused by ZnSO4 salts and increase Zn transport trough leaf petiole. However, under longer evaluation, i.e. a couple of weeks, the root application of ZnSO4 increased by 42% the Zn accumulation in soybean plants compared to the control (low Zn supply). Additionally, root uptake mechanism under hydroponics was more efficient than the foliar application of ZnSO4, ZnO nanoparticles and ZnSO4 + cellulose microspheres. The foliar and root application of Zn did not affect the activity of ezymes related to the metabolism of reactive oxygen species. Understanding the mechanisms ruling the foliar absorption and metabolization of nutrients is fundamental for the development of the next generation of fertilizers. Therefore, more studies on this subject are necessary
publishDate 2021
dc.date.none.fl_str_mv 2021-10-22
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.teses.usp.br/teses/disponiveis/64/64135/tde-31102023-093912/
url https://www.teses.usp.br/teses/disponiveis/64/64135/tde-31102023-093912/
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv
dc.rights.driver.fl_str_mv Liberar o conteúdo para acesso público.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Liberar o conteúdo para acesso público.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv
dc.publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
dc.source.none.fl_str_mv
reponame:Biblioteca Digital de Teses e Dissertações da USP
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Biblioteca Digital de Teses e Dissertações da USP
collection Biblioteca Digital de Teses e Dissertações da USP
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)
repository.mail.fl_str_mv virginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.br
_version_ 1815257520240852992