Ex vivo lipid peroxidation profile of the subjects stratum corneum by the HPLC-TBARS-EVSC protocol

Detalhes bibliográficos
Autor(a) principal: Marques, Gabriela de Argollo
Data de Publicação: 2023
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Biblioteca Digital de Teses e Dissertações da USP
Texto Completo: https://www.teses.usp.br/teses/disponiveis/9/9139/tde-18082023-151307/
Resumo: Background: Considering the need to improve safety and efficacy protocols to evaluate the human stratum corneum (CS) and its interaction with topical and cosmetic formulations by minimally or non-invasive methodologies, the objective of our research work was to improve the performance of the HPLC-TBARS-EVSC methodology (high performance liquid chromatography - thiobarbituric acid reactive substances - stratum corneum ex vivo), exploring the results of a group of subjects. Methods: The study included 18 healthy subjects aged between 19-57 years old (9 females and 9 males) with phototypes from II to V. Two sites in the forearm of each volunteer were randomly delimited and the SC was collected by the tape stripping technique. High performance liquid chromatography (HPLC) was used to quantify the MDA-TBA2 adduct from the tape-stripped SC, irradiated and not by an ultraviolet simulator chamber. Conclusions: Observing the findings of our present investigation, and the statistical approach applied, the use of the ratio between the treatment site and control would be an adequate strategy to better discriminate and evaluate the results. Additionally, an optimal selection of the volunteers to respond specifically to the purpose of the ex vivo assay also can be considered advantageous. It seems that in future studies focusing on the impact of lipid peroxidation induced by UV irradiation, the most suitable subjects are females aged less than 35 years old, with phototype II.
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spelling Ex vivo lipid peroxidation profile of the subjects stratum corneum by the HPLC-TBARS-EVSC protocolPerfil ex vivo da peroxidação lipídica do estrato córneo de participantes de pesquisa por meio do protocolo HPLC-TBARS-EVSCCLAEEstrato córneoFototipoHPLCLipid peroxidationPeroxidação lipídicaPhototypeStratum corneumTBARSTBARSBackground: Considering the need to improve safety and efficacy protocols to evaluate the human stratum corneum (CS) and its interaction with topical and cosmetic formulations by minimally or non-invasive methodologies, the objective of our research work was to improve the performance of the HPLC-TBARS-EVSC methodology (high performance liquid chromatography - thiobarbituric acid reactive substances - stratum corneum ex vivo), exploring the results of a group of subjects. Methods: The study included 18 healthy subjects aged between 19-57 years old (9 females and 9 males) with phototypes from II to V. Two sites in the forearm of each volunteer were randomly delimited and the SC was collected by the tape stripping technique. High performance liquid chromatography (HPLC) was used to quantify the MDA-TBA2 adduct from the tape-stripped SC, irradiated and not by an ultraviolet simulator chamber. Conclusions: Observing the findings of our present investigation, and the statistical approach applied, the use of the ratio between the treatment site and control would be an adequate strategy to better discriminate and evaluate the results. Additionally, an optimal selection of the volunteers to respond specifically to the purpose of the ex vivo assay also can be considered advantageous. It seems that in future studies focusing on the impact of lipid peroxidation induced by UV irradiation, the most suitable subjects are females aged less than 35 years old, with phototype II.Introdução: Considerando a necessidade de melhorar os protocolos de segurança e eficácia para avaliar o estrato córneo humano (SC) e sua interação com formulações tópicas e cosméticas por metodologias minimamente ou não invasivas, o objetivo de nosso trabalho de pesquisa foi melhorar o desempenho da metodologia HPLC-TBARSEVSC (cromatografia líquida de alta eficiência - substâncias reativas ao ácido tiobarbitúrico - estrato córneo ex vivo), explorando os resultados de um grupo de sujeitos. Métodos: O estudo incluiu 18 indivíduos saudáveis com idades entre 19-57 anos (9 mulheres e 9 homens) com fototipos de II a V. Dois locais no antebraço de cada voluntário foram delimitados aleatoriamente e o SC foi coletado pela técnica de decapagem de fita. Cromatografia líquida de alta eficiência (CLAE) foi utilizada para quantificar o aduto MDA-TBA2 a partir do SC despojado de fita, irradiado e não por uma câmara simuladora ultravioleta. Conclusões: Observando os achados de nossa presente investigação e a abordagem estatística aplicada, a utilização da razão entre o local de tratamento e o controle seria uma estratégia adequada para melhor discriminar e avaliar os resultados. Ademais, uma seleção ideal dos voluntários para responder especificamente ao propósito do ensaio ex vivo também pode ser considerada vantajosa. Parece que em estudos futuros com foco no impacto da peroxidação lipídica induzida pela irradiação UV, os sujeitos mais adequados são mulheres com idade inferior a 35 anos, com fototipo II.Biblioteca Digitais de Teses e Dissertações da USPBaby, André RolimMarques, Gabriela de Argollo2023-07-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttps://www.teses.usp.br/teses/disponiveis/9/9139/tde-18082023-151307/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPLiberar o conteúdo para acesso público.info:eu-repo/semantics/openAccesseng2023-08-31T23:08:02Zoai:teses.usp.br:tde-18082023-151307Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212023-08-31T23:08:02Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Ex vivo lipid peroxidation profile of the subjects stratum corneum by the HPLC-TBARS-EVSC protocol
Perfil ex vivo da peroxidação lipídica do estrato córneo de participantes de pesquisa por meio do protocolo HPLC-TBARS-EVSC
title Ex vivo lipid peroxidation profile of the subjects stratum corneum by the HPLC-TBARS-EVSC protocol
spellingShingle Ex vivo lipid peroxidation profile of the subjects stratum corneum by the HPLC-TBARS-EVSC protocol
Marques, Gabriela de Argollo
CLAE
Estrato córneo
Fototipo
HPLC
Lipid peroxidation
Peroxidação lipídica
Phototype
Stratum corneum
TBARS
TBARS
title_short Ex vivo lipid peroxidation profile of the subjects stratum corneum by the HPLC-TBARS-EVSC protocol
title_full Ex vivo lipid peroxidation profile of the subjects stratum corneum by the HPLC-TBARS-EVSC protocol
title_fullStr Ex vivo lipid peroxidation profile of the subjects stratum corneum by the HPLC-TBARS-EVSC protocol
title_full_unstemmed Ex vivo lipid peroxidation profile of the subjects stratum corneum by the HPLC-TBARS-EVSC protocol
title_sort Ex vivo lipid peroxidation profile of the subjects stratum corneum by the HPLC-TBARS-EVSC protocol
author Marques, Gabriela de Argollo
author_facet Marques, Gabriela de Argollo
author_role author
dc.contributor.none.fl_str_mv Baby, André Rolim
dc.contributor.author.fl_str_mv Marques, Gabriela de Argollo
dc.subject.por.fl_str_mv CLAE
Estrato córneo
Fototipo
HPLC
Lipid peroxidation
Peroxidação lipídica
Phototype
Stratum corneum
TBARS
TBARS
topic CLAE
Estrato córneo
Fototipo
HPLC
Lipid peroxidation
Peroxidação lipídica
Phototype
Stratum corneum
TBARS
TBARS
description Background: Considering the need to improve safety and efficacy protocols to evaluate the human stratum corneum (CS) and its interaction with topical and cosmetic formulations by minimally or non-invasive methodologies, the objective of our research work was to improve the performance of the HPLC-TBARS-EVSC methodology (high performance liquid chromatography - thiobarbituric acid reactive substances - stratum corneum ex vivo), exploring the results of a group of subjects. Methods: The study included 18 healthy subjects aged between 19-57 years old (9 females and 9 males) with phototypes from II to V. Two sites in the forearm of each volunteer were randomly delimited and the SC was collected by the tape stripping technique. High performance liquid chromatography (HPLC) was used to quantify the MDA-TBA2 adduct from the tape-stripped SC, irradiated and not by an ultraviolet simulator chamber. Conclusions: Observing the findings of our present investigation, and the statistical approach applied, the use of the ratio between the treatment site and control would be an adequate strategy to better discriminate and evaluate the results. Additionally, an optimal selection of the volunteers to respond specifically to the purpose of the ex vivo assay also can be considered advantageous. It seems that in future studies focusing on the impact of lipid peroxidation induced by UV irradiation, the most suitable subjects are females aged less than 35 years old, with phototype II.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-07
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.teses.usp.br/teses/disponiveis/9/9139/tde-18082023-151307/
url https://www.teses.usp.br/teses/disponiveis/9/9139/tde-18082023-151307/
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv
dc.rights.driver.fl_str_mv Liberar o conteúdo para acesso público.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Liberar o conteúdo para acesso público.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv
dc.publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
dc.source.none.fl_str_mv
reponame:Biblioteca Digital de Teses e Dissertações da USP
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Biblioteca Digital de Teses e Dissertações da USP
collection Biblioteca Digital de Teses e Dissertações da USP
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)
repository.mail.fl_str_mv virginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.br
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