Hemicellulases and acessory proteins from filamentous fungi and actinomycetes for lignocellulose biomass deconstruction.

Detalhes bibliográficos
Autor(a) principal: Robl, Diogo
Data de Publicação: 2015
Tipo de documento: Tese
Idioma: eng
Título da fonte: Biblioteca Digital de Teses e Dissertações da USP
Texto Completo: http://www.teses.usp.br/teses/disponiveis/87/87131/tde-04092015-151800/
Resumo: Endophytic microorganisms were screened for hemicellulases production using plate assays and liquid cultivations. Two strains were selected and used in further studies. Aspergillus niger DR02 strain and Annulohypoxylon stigyum DR47. In A.niger fed-batch submerged cultivation approaches were developed using liquor from hydrothermal sugar cane pretreatment, and maximum xylanase activities obtained were 458.1 U/mL for constant fed-batch mode. For A. stygium DR47 media optimization and bioreactor cultivation using citrus bagasse and soybean bran were explored and revealed a maximum production of 6.26 U/mL of pectinase and 10.13 U/mL of β-glucosidase. Improved hemicellulase production was also done by genetic modifications at carbon catabolic repression levels in A. niger. Mass spectrometric studies were done in enzymatic extracts produced and contributed to understand the enzymatic supplementation of Celluclast 1.5L.In addition the extracts produced were tested for an enzyme formulation and that was able to increase sugar cane bagasse hydrolysis.
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spelling Hemicellulases and acessory proteins from filamentous fungi and actinomycetes for lignocellulose biomass deconstruction.Hemicelulases e proteínas acessórias de fungos filamentosos e de actinomicetos para desconstrução de biomassa lignocelulósica.ActinomicetosActinomycetesCana de açúcarEndofíticosEndophyticsFungiFungosHemicellulasesHemicelulasesSugar caneEndophytic microorganisms were screened for hemicellulases production using plate assays and liquid cultivations. Two strains were selected and used in further studies. Aspergillus niger DR02 strain and Annulohypoxylon stigyum DR47. In A.niger fed-batch submerged cultivation approaches were developed using liquor from hydrothermal sugar cane pretreatment, and maximum xylanase activities obtained were 458.1 U/mL for constant fed-batch mode. For A. stygium DR47 media optimization and bioreactor cultivation using citrus bagasse and soybean bran were explored and revealed a maximum production of 6.26 U/mL of pectinase and 10.13 U/mL of β-glucosidase. Improved hemicellulase production was also done by genetic modifications at carbon catabolic repression levels in A. niger. Mass spectrometric studies were done in enzymatic extracts produced and contributed to understand the enzymatic supplementation of Celluclast 1.5L.In addition the extracts produced were tested for an enzyme formulation and that was able to increase sugar cane bagasse hydrolysis.Microrganismos endofíticos foram selecionados para a produção de hemicelulases através de testes em placa e de cultivo líquido. Duas linhagens foram selecionadas para posteriores cultivos, Aspergillus niger DR02 e Annulohypoxylon stigyum DR47. Para o fungo A.niger batelada alimentada em cultivo submerso com licor do tratamento hidrotérmico do bagaço de cana de açúcar permitiram obtenção de máxima atividade de xilanase de 458,1 U/mL. Para a linhagem A. stygium DR47 otimização do cultivo utilizando bagaço cítrico e farelo de soja foram explorados e revelaram produção de 6,26 U/mL de pectinase e 10,13 U/mL de β-glucosidase. Melhoramento da produção de hemicelulase foi realizado com modificações genéticas ao nível de repressão catabólica de carbono em A. niger. Estudos de espectrometria de massas foram realizados nos extratos enzimáticos produzidos e contribuíram para entender a suplementação da Celluclast 1.5L. Além disso, os extratos produzidos foram testados para uma formulação que foi capaz de aumentar a hidrólise do bagaço de cana de açúcar.Biblioteca Digitais de Teses e Dissertações da USPMaldonado, Gabriel PadillaPradella, Jose Geraldo da CruzRobl, Diogo2015-04-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttp://www.teses.usp.br/teses/disponiveis/87/87131/tde-04092015-151800/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPLiberar o conteúdo para acesso público.info:eu-repo/semantics/openAccesseng2017-09-03T06:00:10Zoai:teses.usp.br:tde-04092015-151800Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212017-09-03T06:00:10Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Hemicellulases and acessory proteins from filamentous fungi and actinomycetes for lignocellulose biomass deconstruction.
Hemicelulases e proteínas acessórias de fungos filamentosos e de actinomicetos para desconstrução de biomassa lignocelulósica.
title Hemicellulases and acessory proteins from filamentous fungi and actinomycetes for lignocellulose biomass deconstruction.
spellingShingle Hemicellulases and acessory proteins from filamentous fungi and actinomycetes for lignocellulose biomass deconstruction.
Robl, Diogo
Actinomicetos
Actinomycetes
Cana de açúcar
Endofíticos
Endophytics
Fungi
Fungos
Hemicellulases
Hemicelulases
Sugar cane
title_short Hemicellulases and acessory proteins from filamentous fungi and actinomycetes for lignocellulose biomass deconstruction.
title_full Hemicellulases and acessory proteins from filamentous fungi and actinomycetes for lignocellulose biomass deconstruction.
title_fullStr Hemicellulases and acessory proteins from filamentous fungi and actinomycetes for lignocellulose biomass deconstruction.
title_full_unstemmed Hemicellulases and acessory proteins from filamentous fungi and actinomycetes for lignocellulose biomass deconstruction.
title_sort Hemicellulases and acessory proteins from filamentous fungi and actinomycetes for lignocellulose biomass deconstruction.
author Robl, Diogo
author_facet Robl, Diogo
author_role author
dc.contributor.none.fl_str_mv Maldonado, Gabriel Padilla
Pradella, Jose Geraldo da Cruz
dc.contributor.author.fl_str_mv Robl, Diogo
dc.subject.por.fl_str_mv Actinomicetos
Actinomycetes
Cana de açúcar
Endofíticos
Endophytics
Fungi
Fungos
Hemicellulases
Hemicelulases
Sugar cane
topic Actinomicetos
Actinomycetes
Cana de açúcar
Endofíticos
Endophytics
Fungi
Fungos
Hemicellulases
Hemicelulases
Sugar cane
description Endophytic microorganisms were screened for hemicellulases production using plate assays and liquid cultivations. Two strains were selected and used in further studies. Aspergillus niger DR02 strain and Annulohypoxylon stigyum DR47. In A.niger fed-batch submerged cultivation approaches were developed using liquor from hydrothermal sugar cane pretreatment, and maximum xylanase activities obtained were 458.1 U/mL for constant fed-batch mode. For A. stygium DR47 media optimization and bioreactor cultivation using citrus bagasse and soybean bran were explored and revealed a maximum production of 6.26 U/mL of pectinase and 10.13 U/mL of β-glucosidase. Improved hemicellulase production was also done by genetic modifications at carbon catabolic repression levels in A. niger. Mass spectrometric studies were done in enzymatic extracts produced and contributed to understand the enzymatic supplementation of Celluclast 1.5L.In addition the extracts produced were tested for an enzyme formulation and that was able to increase sugar cane bagasse hydrolysis.
publishDate 2015
dc.date.none.fl_str_mv 2015-04-30
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.teses.usp.br/teses/disponiveis/87/87131/tde-04092015-151800/
url http://www.teses.usp.br/teses/disponiveis/87/87131/tde-04092015-151800/
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv
dc.rights.driver.fl_str_mv Liberar o conteúdo para acesso público.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Liberar o conteúdo para acesso público.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv
dc.publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
dc.source.none.fl_str_mv
reponame:Biblioteca Digital de Teses e Dissertações da USP
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Biblioteca Digital de Teses e Dissertações da USP
collection Biblioteca Digital de Teses e Dissertações da USP
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)
repository.mail.fl_str_mv virginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.br
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