Integrative taxonomy to clarify the identity of Anastrepha dissimilis Stone, 1942 (Diptera: Tephritidae), with an illustrated key to the species of Anastrepha pseudoparallela group recorded on passion fruit (Passiflora sp.) in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da USP |
Texto Completo: | https://www.teses.usp.br/teses/disponiveis/11/11146/tde-11052022-110916/ |
Resumo: | The fruit flies of the genus Anastrepha Schiner comprise a group of phytophagous Diptera. Some species are pests of fruticulture, as they use economically significant fruits as a nutritional resource for the larval stage. Taxonomically, the genus is divided into multiple groups of species, according to morphological and molecular evidence. The pseudoparallela group comprises 31 described species, 11 of which are registered in Brazil. Species of the pseudoparallela group mainly infest passion fruits (Passiflora spp.). However, in Brazil, the taxonomic knowledge of these species is still limited. So far, there is no taxonomic key for identifying the species of this group recorded in passion fruit in Brazil. Furthermore, most study of species in this group has focused on species that attack fruits of commercial species of Passiflora, such as Anastrepha consobrina and A. pseudoparallela. Among the poorly known species of the pseudoparallela group, is Anastrepha dissimilis Stone, 1942, which has a wide distribution in Brazil, occurring in 20 states. Morphological comparisons of the A. dissimilis paratype from Bonito, Pernambuco, Brazil with the holotype collected in Plaisance, Haiti, suggest that more than one species is confused under a single nominal species. Therefore, this research aims to carry out a taxonomic study of Brazilian populations of A. dissimilis using an integrative approach and to produce an illustrated key to the Brazilian species of the pseudoparallela group recorded in passion fruit. The specific objectives of this thesis plan are: (1) to compare the morphology of the aculeus of A. dissimilis from multiple localities; (2) to carry out a morphometric study of the wing, aculeus, and mesonotum of Brazilian populations of A. dissimilis, using linear and geometric analysis; (3) to perform a molecular study of Brazilian populations of A. dissimilis, using the subunit I of the mitochondrial gene Cytochrome Oxidase (COI); (4) to develop an illustrated key to the species of the pseudoparallela group recorded in passion fruit in Brazil; and (5) to enhance the collection of the Museu de Entomologia Luiz de Queiroz (MELQ) of the Escola Superior de Agricultura Luiz de Queiroz (ESALQ/USP) with the specimens collected and identified in the study. Morphological and molecular analyses of specimens from different localities in Brazil, that would be identified as A. dissimilis using the current identification key, suggested that they are A. chiclayae Greene. Furthermore, the morphometric study showed considerable morphological variation among several populations, suggesting the existence of at least two morphospecies. Thus, in this study, the integrative taxonomic approach allowed us to register, for the first time, the occurrence of A. chiclayae in Brazil. In addition, a checklist of species of the A. pseudoparallela group registered in passion fruit in Brazil was prepared using data from the literature. Finally, an illustrated taxonomic key for identifying these species was developed. |
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Integrative taxonomy to clarify the identity of Anastrepha dissimilis Stone, 1942 (Diptera: Tephritidae), with an illustrated key to the species of Anastrepha pseudoparallela group recorded on passion fruit (Passiflora sp.) in BrazilTaxonomia integrativa para esclarecer a identidade de Anastrepha dissimilis Stone, 1942 (Diptera: Tephritidae) e chave ilustrada para as espécies brasileiras do grupo Anastrepha pseudoparallela registradas em maracujá (Passiflora sp.) no BrasilFruit fliesMaracujáMoscas-das-frutasPassion fruitTaxonomiaTaxonomyTrypetinaeTrypetinaeThe fruit flies of the genus Anastrepha Schiner comprise a group of phytophagous Diptera. Some species are pests of fruticulture, as they use economically significant fruits as a nutritional resource for the larval stage. Taxonomically, the genus is divided into multiple groups of species, according to morphological and molecular evidence. The pseudoparallela group comprises 31 described species, 11 of which are registered in Brazil. Species of the pseudoparallela group mainly infest passion fruits (Passiflora spp.). However, in Brazil, the taxonomic knowledge of these species is still limited. So far, there is no taxonomic key for identifying the species of this group recorded in passion fruit in Brazil. Furthermore, most study of species in this group has focused on species that attack fruits of commercial species of Passiflora, such as Anastrepha consobrina and A. pseudoparallela. Among the poorly known species of the pseudoparallela group, is Anastrepha dissimilis Stone, 1942, which has a wide distribution in Brazil, occurring in 20 states. Morphological comparisons of the A. dissimilis paratype from Bonito, Pernambuco, Brazil with the holotype collected in Plaisance, Haiti, suggest that more than one species is confused under a single nominal species. Therefore, this research aims to carry out a taxonomic study of Brazilian populations of A. dissimilis using an integrative approach and to produce an illustrated key to the Brazilian species of the pseudoparallela group recorded in passion fruit. The specific objectives of this thesis plan are: (1) to compare the morphology of the aculeus of A. dissimilis from multiple localities; (2) to carry out a morphometric study of the wing, aculeus, and mesonotum of Brazilian populations of A. dissimilis, using linear and geometric analysis; (3) to perform a molecular study of Brazilian populations of A. dissimilis, using the subunit I of the mitochondrial gene Cytochrome Oxidase (COI); (4) to develop an illustrated key to the species of the pseudoparallela group recorded in passion fruit in Brazil; and (5) to enhance the collection of the Museu de Entomologia Luiz de Queiroz (MELQ) of the Escola Superior de Agricultura Luiz de Queiroz (ESALQ/USP) with the specimens collected and identified in the study. Morphological and molecular analyses of specimens from different localities in Brazil, that would be identified as A. dissimilis using the current identification key, suggested that they are A. chiclayae Greene. Furthermore, the morphometric study showed considerable morphological variation among several populations, suggesting the existence of at least two morphospecies. Thus, in this study, the integrative taxonomic approach allowed us to register, for the first time, the occurrence of A. chiclayae in Brazil. In addition, a checklist of species of the A. pseudoparallela group registered in passion fruit in Brazil was prepared using data from the literature. Finally, an illustrated taxonomic key for identifying these species was developed.O gênero Anastrepha Schiner, de moscas-das-frutas, compreende um grupo de dípteros fitófagos e algumas espécies são pragas da fruticultura, já que utilizam frutos de importância econômica como recurso nutricional para o estágio larval. Taxonomicamente, o gênero está dividido em vários grupos de espécies, de acordo com evidências morfológicas e moleculares. O grupo pseudoparallela compreende um total de 31 espécies descritas, sendo 11 registradas no Brasil. Geralmente, as espécies desse grupo são conhecidas por infestarem frutos de várias espécies de maracujazeiro (Passiflora spp.). No Brasil, o conhecimento taxonômico do grupo pseudoparallela ainda é limitado e não existia, até então, uma chave taxonômica para a identificação das espécies registradas em maracujá. Além disso, o estudo das espécies desse grupo é restrito àquelas que atacam frutos de espécies comerciais de Passiflora, como Anastrepha consobrina e A. pseudoparallela. Entre as espécies pouco conhecidas do grupo, está Anastrepha dissimilis Stone, 1942, que possui ampla distribuição em território brasileiro, ocorrendo em 20 estados. Comparações morfológicas entre um parátipo de A. dissimilis coletado em Bonito, Pernambuco, Brasil com o holótipo, coletado em Plaisance, Haiti, sugere que os espécimes brasileiros e o holótipo não se tratam da mesma espécie. Portanto, o objetivo deste trabalho foi realizar um estudo taxonômico de populações brasileiras de A. dissimilis sob uma abordagem integrativa e elaborar uma chave ilustrada para as espécies brasileiras do grupo pseudoparallela registradas em maracujá. Os objetivos específicos deste estudo foram: (1) comparar a morfologia do acúleo das fêmeas de diferentes populações brasileiras de A. dissimilis; (2) realizar um estudo morfométrico das asas, acúleo e mesonoto de populações brasileiras de A. dissimilis, utilizando a morfometria linear e geométrica; (3) investigar a variabilidade molecular entre populações brasileiras de A. dissimilis, utilizando a subunidade I do gene mitocondrial Citocromo Oxidase (COI); (4) elaborar uma chave ilustrada para a identificação das espécies do grupo pseudoparallela registradas em maracujá no Brasil e (5) incrementar e melhorar a curadoria da coleção do Museu de Entomologia Luiz de Queiroz (MELQ) da Escola Superior de Agricultura Luiz de Queiroz (ESALQ/USP) com os espécimes estudados. As análises morfológicas e moleculares dos espécimes de A. dissimilis oriundas de diversas localidades do Brasil sugeriram que, na verdade, se tratam de A. chiclayae. Além disso, o estudo morfométrico foi capaz de evidenciar uma considerável variação morfológica entre as diferentes populações, mostrando a existência de, pelo menos, duas morfoespécies. Desta forma, com a abordagem taxonômica integrativa, é registrada pela primeira vez para o Brasil, a espécie A. chiclayae Greene. Além disso, uma lista comentada das espécies do grupo A. pseudoparallela, registradas em maracujá no Brasil, foi realizada com base nos dados da literatura. Por fim, uma chave taxonômica ilustrada para a identificação dessas espécies foi elaborada.Biblioteca Digitais de Teses e Dissertações da USPSavaris, MarcoandreAraújo, Alexandre Santos2022-02-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttps://www.teses.usp.br/teses/disponiveis/11/11146/tde-11052022-110916/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPLiberar o conteúdo para acesso público.info:eu-repo/semantics/openAccesseng2022-05-12T11:18:40Zoai:teses.usp.br:tde-11052022-110916Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212022-05-12T11:18:40Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Integrative taxonomy to clarify the identity of Anastrepha dissimilis Stone, 1942 (Diptera: Tephritidae), with an illustrated key to the species of Anastrepha pseudoparallela group recorded on passion fruit (Passiflora sp.) in Brazil Taxonomia integrativa para esclarecer a identidade de Anastrepha dissimilis Stone, 1942 (Diptera: Tephritidae) e chave ilustrada para as espécies brasileiras do grupo Anastrepha pseudoparallela registradas em maracujá (Passiflora sp.) no Brasil |
title |
Integrative taxonomy to clarify the identity of Anastrepha dissimilis Stone, 1942 (Diptera: Tephritidae), with an illustrated key to the species of Anastrepha pseudoparallela group recorded on passion fruit (Passiflora sp.) in Brazil |
spellingShingle |
Integrative taxonomy to clarify the identity of Anastrepha dissimilis Stone, 1942 (Diptera: Tephritidae), with an illustrated key to the species of Anastrepha pseudoparallela group recorded on passion fruit (Passiflora sp.) in Brazil Araújo, Alexandre Santos Fruit flies Maracujá Moscas-das-frutas Passion fruit Taxonomia Taxonomy Trypetinae Trypetinae |
title_short |
Integrative taxonomy to clarify the identity of Anastrepha dissimilis Stone, 1942 (Diptera: Tephritidae), with an illustrated key to the species of Anastrepha pseudoparallela group recorded on passion fruit (Passiflora sp.) in Brazil |
title_full |
Integrative taxonomy to clarify the identity of Anastrepha dissimilis Stone, 1942 (Diptera: Tephritidae), with an illustrated key to the species of Anastrepha pseudoparallela group recorded on passion fruit (Passiflora sp.) in Brazil |
title_fullStr |
Integrative taxonomy to clarify the identity of Anastrepha dissimilis Stone, 1942 (Diptera: Tephritidae), with an illustrated key to the species of Anastrepha pseudoparallela group recorded on passion fruit (Passiflora sp.) in Brazil |
title_full_unstemmed |
Integrative taxonomy to clarify the identity of Anastrepha dissimilis Stone, 1942 (Diptera: Tephritidae), with an illustrated key to the species of Anastrepha pseudoparallela group recorded on passion fruit (Passiflora sp.) in Brazil |
title_sort |
Integrative taxonomy to clarify the identity of Anastrepha dissimilis Stone, 1942 (Diptera: Tephritidae), with an illustrated key to the species of Anastrepha pseudoparallela group recorded on passion fruit (Passiflora sp.) in Brazil |
author |
Araújo, Alexandre Santos |
author_facet |
Araújo, Alexandre Santos |
author_role |
author |
dc.contributor.none.fl_str_mv |
Savaris, Marcoandre |
dc.contributor.author.fl_str_mv |
Araújo, Alexandre Santos |
dc.subject.por.fl_str_mv |
Fruit flies Maracujá Moscas-das-frutas Passion fruit Taxonomia Taxonomy Trypetinae Trypetinae |
topic |
Fruit flies Maracujá Moscas-das-frutas Passion fruit Taxonomia Taxonomy Trypetinae Trypetinae |
description |
The fruit flies of the genus Anastrepha Schiner comprise a group of phytophagous Diptera. Some species are pests of fruticulture, as they use economically significant fruits as a nutritional resource for the larval stage. Taxonomically, the genus is divided into multiple groups of species, according to morphological and molecular evidence. The pseudoparallela group comprises 31 described species, 11 of which are registered in Brazil. Species of the pseudoparallela group mainly infest passion fruits (Passiflora spp.). However, in Brazil, the taxonomic knowledge of these species is still limited. So far, there is no taxonomic key for identifying the species of this group recorded in passion fruit in Brazil. Furthermore, most study of species in this group has focused on species that attack fruits of commercial species of Passiflora, such as Anastrepha consobrina and A. pseudoparallela. Among the poorly known species of the pseudoparallela group, is Anastrepha dissimilis Stone, 1942, which has a wide distribution in Brazil, occurring in 20 states. Morphological comparisons of the A. dissimilis paratype from Bonito, Pernambuco, Brazil with the holotype collected in Plaisance, Haiti, suggest that more than one species is confused under a single nominal species. Therefore, this research aims to carry out a taxonomic study of Brazilian populations of A. dissimilis using an integrative approach and to produce an illustrated key to the Brazilian species of the pseudoparallela group recorded in passion fruit. The specific objectives of this thesis plan are: (1) to compare the morphology of the aculeus of A. dissimilis from multiple localities; (2) to carry out a morphometric study of the wing, aculeus, and mesonotum of Brazilian populations of A. dissimilis, using linear and geometric analysis; (3) to perform a molecular study of Brazilian populations of A. dissimilis, using the subunit I of the mitochondrial gene Cytochrome Oxidase (COI); (4) to develop an illustrated key to the species of the pseudoparallela group recorded in passion fruit in Brazil; and (5) to enhance the collection of the Museu de Entomologia Luiz de Queiroz (MELQ) of the Escola Superior de Agricultura Luiz de Queiroz (ESALQ/USP) with the specimens collected and identified in the study. Morphological and molecular analyses of specimens from different localities in Brazil, that would be identified as A. dissimilis using the current identification key, suggested that they are A. chiclayae Greene. Furthermore, the morphometric study showed considerable morphological variation among several populations, suggesting the existence of at least two morphospecies. Thus, in this study, the integrative taxonomic approach allowed us to register, for the first time, the occurrence of A. chiclayae in Brazil. In addition, a checklist of species of the A. pseudoparallela group registered in passion fruit in Brazil was prepared using data from the literature. Finally, an illustrated taxonomic key for identifying these species was developed. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-02-21 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.teses.usp.br/teses/disponiveis/11/11146/tde-11052022-110916/ |
url |
https://www.teses.usp.br/teses/disponiveis/11/11146/tde-11052022-110916/ |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
|
dc.rights.driver.fl_str_mv |
Liberar o conteúdo para acesso público. info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Liberar o conteúdo para acesso público. |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
|
dc.publisher.none.fl_str_mv |
Biblioteca Digitais de Teses e Dissertações da USP |
publisher.none.fl_str_mv |
Biblioteca Digitais de Teses e Dissertações da USP |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da USP instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Biblioteca Digital de Teses e Dissertações da USP |
collection |
Biblioteca Digital de Teses e Dissertações da USP |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
virginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.br |
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1809090615787388928 |