Use of Doppler ultrasonography for selection of recipients in embryo transfer programs in horses
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da USP |
Texto Completo: | https://www.teses.usp.br/teses/disponiveis/10/10131/tde-25052023-113534/ |
Resumo: | We aimed to evaluate the impact of corpus luteum (CL) and uterine characteristics accessed by B-mode and color Doppler ultrasonography in recipient mares at the time of embryo transfer (ET) on pregnancy outcomes. Recipient mares (n=110), between days 3 to 9 after spontaneous ovulation, received a fresh embryo. Immediately before ET, the reproductive system was assessed by transrectal palpation for the following parameters: uterine tone (0-3), CL echogenicity (0-6), CL type (homogeneous, trabecular or anechoic center), luteal area (cm2), uterine echogenicity (0-3), uterine edema (0-3), uterine echotexture (0-3), luteal blood perfusion (0-100%) and uterine blood perfusion (1-4). Additionally, a blood sample was collected by puncture of the jugular vein for plasma P4 dosage. Retrospectively, recipients were classified according to the luteal area (small [≤ 6 cm2] or large [> 6 cm2]), luteal blood perfusion (low [≤ 55%] or high [> 55%]), and plasma concentration of P4 (low ≤ 9.98 ng/mL or high > 9.98 ng/mL). Pregnancy diagnosis was performed at 15 and 30 days of gestation. Luteal blood perfusion was significantly higher (P= 0.03) in pregnant recipients (n= 83) than in non-pregnant recipients (n= 27). Overall P/ET was higher (P≤ 0.02) in mares with high luteal blood perfusion and high P4. Luteal blood perfusion was the most adequate significant (P= 0.01) predictor of pregnancy compared with the luteal area and plasma P4 concentration. Only luteal blood perfusion showed a linear (P= 0.03) and cubic (P= 0.004) effect on P/ET. In conclusion, CL blood perfusion determined by color-Doppler can be used in real-time to select recipients with the greatest chance of maintaining pregnancy in equine ET programs. |
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Use of Doppler ultrasonography for selection of recipients in embryo transfer programs in horsesUso da ultrassonografia Doppler na seleção de receptoras em programas de transferência de embriões em equinosBlood perfusionColor dopplerCorpo lúteoCorpus luteumDoppler coloridoÉguas receptorasPerfusão sanguíneaRecipient maresWe aimed to evaluate the impact of corpus luteum (CL) and uterine characteristics accessed by B-mode and color Doppler ultrasonography in recipient mares at the time of embryo transfer (ET) on pregnancy outcomes. Recipient mares (n=110), between days 3 to 9 after spontaneous ovulation, received a fresh embryo. Immediately before ET, the reproductive system was assessed by transrectal palpation for the following parameters: uterine tone (0-3), CL echogenicity (0-6), CL type (homogeneous, trabecular or anechoic center), luteal area (cm2), uterine echogenicity (0-3), uterine edema (0-3), uterine echotexture (0-3), luteal blood perfusion (0-100%) and uterine blood perfusion (1-4). Additionally, a blood sample was collected by puncture of the jugular vein for plasma P4 dosage. Retrospectively, recipients were classified according to the luteal area (small [≤ 6 cm2] or large [> 6 cm2]), luteal blood perfusion (low [≤ 55%] or high [> 55%]), and plasma concentration of P4 (low ≤ 9.98 ng/mL or high > 9.98 ng/mL). Pregnancy diagnosis was performed at 15 and 30 days of gestation. Luteal blood perfusion was significantly higher (P= 0.03) in pregnant recipients (n= 83) than in non-pregnant recipients (n= 27). Overall P/ET was higher (P≤ 0.02) in mares with high luteal blood perfusion and high P4. Luteal blood perfusion was the most adequate significant (P= 0.01) predictor of pregnancy compared with the luteal area and plasma P4 concentration. Only luteal blood perfusion showed a linear (P= 0.03) and cubic (P= 0.004) effect on P/ET. In conclusion, CL blood perfusion determined by color-Doppler can be used in real-time to select recipients with the greatest chance of maintaining pregnancy in equine ET programs.Objetivamos avaliar o impacto das características do corpo lúteo (CL) e do útero acessadas pela ultrassonografia em modo-B e Doppler-colorido em éguas receptoras no momento da transferência de embriões (TE) sobre os resultados da prenhez em um programa de TE. Éguas receptoras (n= 110), entre dias 3 a 9 após a ovulação espontânea, receberam um embrião fresco. Imediatamente antes da TE, o sistema reprodutor foi avaliado por palpação transretal quanto aos parâmetros a seguir: tônus uterino (0-3), ecogenicidade do CL (0-6), tipo de CL (homogêneo, trabeculado ou centro anecoico), área lútea (cm2 ), ecogenicidade uterina (0-3), edema uterino (0-3), ecotextura uterina (0-3), perfusão sanguínea luteal (0-100%) e perfusão sanguínea uterina (1-4). Adicionalmente, uma amostra de sangue foi coletada por punção da veia jugular para dosagem plasmática de P4. Restrospectivamente, as receptoras foram classificadas de acordo com a área luteal (pequeno [≤ 6 cm2 ] ou grande [> 6 cm2 ]), perfusão sanguínea luteal (baixa [≤ 55%] ou alta [> 55%]) e concentração plasmática de P4 (baixa ≤ 9,98 ng/mL ou alto > 9,98 ng/mL). O diagnóstico de gestação foi realizado aos 15 e 30 dias de gestação. A perfusão sanguínea luteal foi significativamente maior (P= 0,03) em receptoras gestantes (n= 83) do que em receptoras não-gestantes (n= 27). A P/TE foi estatisticamente superior (P≤ 0,02) em éguas com perfusão sanguínea luteal e concentração plasmática de P4 altas. A perfusão sanguínea luteal foi o preditor significativo (P= 0,01) mais adequado da prenhez em comparação com a área luteal e concentração plasmática de P4. Adicionalmente, somente a perfusão sanguínea luteal apresentou efeito linear (P= 0,03) e cúbico (P= 0,004) sobre a P/TE. Em conclusão, a perfusão sanguínea do CL determinada pelo Doppler-colorido pode ser utilizada em tempo real para selecionar as receptoras com maiores chances de manter a prenhez em programas de TE em equinos.Biblioteca Digitais de Teses e Dissertações da USPPugliesi, GuilhermeMorelli, Karine Galhego2023-03-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttps://www.teses.usp.br/teses/disponiveis/10/10131/tde-25052023-113534/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPLiberar o conteúdo para acesso público.info:eu-repo/semantics/openAccesseng2023-09-29T13:10:03Zoai:teses.usp.br:tde-25052023-113534Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212023-09-29T13:10:03Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Use of Doppler ultrasonography for selection of recipients in embryo transfer programs in horses Uso da ultrassonografia Doppler na seleção de receptoras em programas de transferência de embriões em equinos |
title |
Use of Doppler ultrasonography for selection of recipients in embryo transfer programs in horses |
spellingShingle |
Use of Doppler ultrasonography for selection of recipients in embryo transfer programs in horses Morelli, Karine Galhego Blood perfusion Color doppler Corpo lúteo Corpus luteum Doppler colorido Éguas receptoras Perfusão sanguínea Recipient mares |
title_short |
Use of Doppler ultrasonography for selection of recipients in embryo transfer programs in horses |
title_full |
Use of Doppler ultrasonography for selection of recipients in embryo transfer programs in horses |
title_fullStr |
Use of Doppler ultrasonography for selection of recipients in embryo transfer programs in horses |
title_full_unstemmed |
Use of Doppler ultrasonography for selection of recipients in embryo transfer programs in horses |
title_sort |
Use of Doppler ultrasonography for selection of recipients in embryo transfer programs in horses |
author |
Morelli, Karine Galhego |
author_facet |
Morelli, Karine Galhego |
author_role |
author |
dc.contributor.none.fl_str_mv |
Pugliesi, Guilherme |
dc.contributor.author.fl_str_mv |
Morelli, Karine Galhego |
dc.subject.por.fl_str_mv |
Blood perfusion Color doppler Corpo lúteo Corpus luteum Doppler colorido Éguas receptoras Perfusão sanguínea Recipient mares |
topic |
Blood perfusion Color doppler Corpo lúteo Corpus luteum Doppler colorido Éguas receptoras Perfusão sanguínea Recipient mares |
description |
We aimed to evaluate the impact of corpus luteum (CL) and uterine characteristics accessed by B-mode and color Doppler ultrasonography in recipient mares at the time of embryo transfer (ET) on pregnancy outcomes. Recipient mares (n=110), between days 3 to 9 after spontaneous ovulation, received a fresh embryo. Immediately before ET, the reproductive system was assessed by transrectal palpation for the following parameters: uterine tone (0-3), CL echogenicity (0-6), CL type (homogeneous, trabecular or anechoic center), luteal area (cm2), uterine echogenicity (0-3), uterine edema (0-3), uterine echotexture (0-3), luteal blood perfusion (0-100%) and uterine blood perfusion (1-4). Additionally, a blood sample was collected by puncture of the jugular vein for plasma P4 dosage. Retrospectively, recipients were classified according to the luteal area (small [≤ 6 cm2] or large [> 6 cm2]), luteal blood perfusion (low [≤ 55%] or high [> 55%]), and plasma concentration of P4 (low ≤ 9.98 ng/mL or high > 9.98 ng/mL). Pregnancy diagnosis was performed at 15 and 30 days of gestation. Luteal blood perfusion was significantly higher (P= 0.03) in pregnant recipients (n= 83) than in non-pregnant recipients (n= 27). Overall P/ET was higher (P≤ 0.02) in mares with high luteal blood perfusion and high P4. Luteal blood perfusion was the most adequate significant (P= 0.01) predictor of pregnancy compared with the luteal area and plasma P4 concentration. Only luteal blood perfusion showed a linear (P= 0.03) and cubic (P= 0.004) effect on P/ET. In conclusion, CL blood perfusion determined by color-Doppler can be used in real-time to select recipients with the greatest chance of maintaining pregnancy in equine ET programs. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-03-17 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.teses.usp.br/teses/disponiveis/10/10131/tde-25052023-113534/ |
url |
https://www.teses.usp.br/teses/disponiveis/10/10131/tde-25052023-113534/ |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
|
dc.rights.driver.fl_str_mv |
Liberar o conteúdo para acesso público. info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Liberar o conteúdo para acesso público. |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
|
dc.publisher.none.fl_str_mv |
Biblioteca Digitais de Teses e Dissertações da USP |
publisher.none.fl_str_mv |
Biblioteca Digitais de Teses e Dissertações da USP |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da USP instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Biblioteca Digital de Teses e Dissertações da USP |
collection |
Biblioteca Digital de Teses e Dissertações da USP |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
virginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.br |
_version_ |
1809091065619152896 |