Antifungal activity of essential oils associated with edible coatings for the postharvest control of spoilage fungi in strawberry (Fragaria spp.)

Detalhes bibliográficos
Autor(a) principal: Oliveira, Jacqueline de
Data de Publicação: 2019
Tipo de documento: Tese
Idioma: eng
Título da fonte: Biblioteca Digital de Teses e Dissertações da USP
Texto Completo: http://www.teses.usp.br/teses/disponiveis/11/11141/tde-24052019-145152/
Resumo: Strawberry cultivation presents as limiting problem the phytosanitary management, which, associated with the residues of the toxic fungicides, the resistance of pathogens and the fragility of the fruit during transport and storage, make the commercialization of this product a challenge. The identification and development of new products with antifungal action, such as essential oils (EOs), is a promising alternative. Therefore, the present study addresses: the in vitro antifungal activity of three essential oils, Eucalyptus staigeriana, Lippia sidoides and Pimenta pseudocaryophyllus; the chemical composition and the mode of action of the EO with the highest potential and its in vivo activity when associated with carboxymethylcellulose (CMC), as well as its effects on the sensory quality and postharvest of strawberries. In chapters two, three and four, the studies were directed to Colletotrichum acutatum, Rhizopus stolonifer and Botrytis cinerea, respectively. In the last chapter, the antifungal activity of a commercial, alternative formulation with low toxicity was evaluated against the same three pathogens. Among the EOs evaluated, the one from L. sidoides presented the best antifungal activity, with the predominance of thymol in its composition, causing morphological alterations in the pathogens. in vivo, the strawberries treated with CMC associated to the EO presented a reduction in the severity of the diseases and improvements in the physicochemical properties with little alteration in the sensory quality of the fruit. The commercial formulation also presented a potential for the control, in vitro and in vivo, of the main postharvest strawberry-deteriorating fungi.
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spelling Antifungal activity of essential oils associated with edible coatings for the postharvest control of spoilage fungi in strawberry (Fragaria spp.)Atividade antifúngica de óleos essenciais associados a recobrimentos comestíveis para o controle pós-colheita de fungos deterioradores do morango (Fragaria spp.)Botrytis cinereaBotrytis cinereaColetotrichum acutatumColetotrichum acutatumLippia sidoidesLippia sidoidesRhizopus stoloniferRhizopus stoloniferCarboxymethylcelluloseStrawberry cultivation presents as limiting problem the phytosanitary management, which, associated with the residues of the toxic fungicides, the resistance of pathogens and the fragility of the fruit during transport and storage, make the commercialization of this product a challenge. The identification and development of new products with antifungal action, such as essential oils (EOs), is a promising alternative. Therefore, the present study addresses: the in vitro antifungal activity of three essential oils, Eucalyptus staigeriana, Lippia sidoides and Pimenta pseudocaryophyllus; the chemical composition and the mode of action of the EO with the highest potential and its in vivo activity when associated with carboxymethylcellulose (CMC), as well as its effects on the sensory quality and postharvest of strawberries. In chapters two, three and four, the studies were directed to Colletotrichum acutatum, Rhizopus stolonifer and Botrytis cinerea, respectively. In the last chapter, the antifungal activity of a commercial, alternative formulation with low toxicity was evaluated against the same three pathogens. Among the EOs evaluated, the one from L. sidoides presented the best antifungal activity, with the predominance of thymol in its composition, causing morphological alterations in the pathogens. in vivo, the strawberries treated with CMC associated to the EO presented a reduction in the severity of the diseases and improvements in the physicochemical properties with little alteration in the sensory quality of the fruit. The commercial formulation also presented a potential for the control, in vitro and in vivo, of the main postharvest strawberry-deteriorating fungi.A cultura do morangueiro tem como problemática limitante o manejo fitossanitário, o que associado aos resíduos dos fungicidas tóxicos, à resistência dos patógenos e à fragilidade dos frutos durante o transporte e armazenamento, tornam a comercialização deste produto um desafio. A identificação e o desenvolvimento de novos produtos com ação antifúngica, como óleos essenciais (OEs), é uma alternativa promissora. Assim, o presente estudo discute: a atividade antifúngica in vitro de três óleos essenciais, Eucalyptus staigeriana, Lippia sidoides e Pimenta pseudocaryophyllus; a composição química e o modo de ação do OE de maior potencial e sua atividade in vivo quando associado com carboximetilcelulose (CMC), assim como seus efeitos na qualidade sensorial e pós-colheita dos morangos. Nos capítulos dois, três e quatro os estudos foram direcionados para Colletotrichum acutatum, Rhizopus stolonifer e Botrytis cinerea, respectivamente. No último capítulo avaliou-se a atividade antifúngica de uma formulação comercial, alternativa e de baixa toxidez contra os mesmos três patógenos. Dentre os OEs avaliados, o de L. sidoides apresentou melhor atividade antifúngica, com a predominância do timol em sua composição, causando alterações morfológicas nos patógenos. in vivo, os morangos tratados com CMC associado ao OE apresentaram redução na severidade das doenças e melhorias nas propriedades físico-químicas com pouca alteração na qualidade sensorial da fruta. A formulação comercial também apresentou potencial de controle, in vitro e in vivo, dos principais fungos deteriorantes pós-coheita do morango.Biblioteca Digitais de Teses e Dissertações da USPSpoto, Marta Helena FilletOliveira, Jacqueline de2019-02-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttp://www.teses.usp.br/teses/disponiveis/11/11141/tde-24052019-145152/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPLiberar o conteúdo para acesso público.info:eu-repo/semantics/openAccesseng2019-06-07T17:56:45Zoai:teses.usp.br:tde-24052019-145152Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212019-06-07T17:56:45Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Antifungal activity of essential oils associated with edible coatings for the postharvest control of spoilage fungi in strawberry (Fragaria spp.)
Atividade antifúngica de óleos essenciais associados a recobrimentos comestíveis para o controle pós-colheita de fungos deterioradores do morango (Fragaria spp.)
title Antifungal activity of essential oils associated with edible coatings for the postharvest control of spoilage fungi in strawberry (Fragaria spp.)
spellingShingle Antifungal activity of essential oils associated with edible coatings for the postharvest control of spoilage fungi in strawberry (Fragaria spp.)
Oliveira, Jacqueline de
Botrytis cinerea
Botrytis cinerea
Coletotrichum acutatum
Coletotrichum acutatum
Lippia sidoides
Lippia sidoides
Rhizopus stolonifer
Rhizopus stolonifer
Carboxymethylcellulose
title_short Antifungal activity of essential oils associated with edible coatings for the postharvest control of spoilage fungi in strawberry (Fragaria spp.)
title_full Antifungal activity of essential oils associated with edible coatings for the postharvest control of spoilage fungi in strawberry (Fragaria spp.)
title_fullStr Antifungal activity of essential oils associated with edible coatings for the postharvest control of spoilage fungi in strawberry (Fragaria spp.)
title_full_unstemmed Antifungal activity of essential oils associated with edible coatings for the postharvest control of spoilage fungi in strawberry (Fragaria spp.)
title_sort Antifungal activity of essential oils associated with edible coatings for the postharvest control of spoilage fungi in strawberry (Fragaria spp.)
author Oliveira, Jacqueline de
author_facet Oliveira, Jacqueline de
author_role author
dc.contributor.none.fl_str_mv Spoto, Marta Helena Fillet
dc.contributor.author.fl_str_mv Oliveira, Jacqueline de
dc.subject.por.fl_str_mv Botrytis cinerea
Botrytis cinerea
Coletotrichum acutatum
Coletotrichum acutatum
Lippia sidoides
Lippia sidoides
Rhizopus stolonifer
Rhizopus stolonifer
Carboxymethylcellulose
topic Botrytis cinerea
Botrytis cinerea
Coletotrichum acutatum
Coletotrichum acutatum
Lippia sidoides
Lippia sidoides
Rhizopus stolonifer
Rhizopus stolonifer
Carboxymethylcellulose
description Strawberry cultivation presents as limiting problem the phytosanitary management, which, associated with the residues of the toxic fungicides, the resistance of pathogens and the fragility of the fruit during transport and storage, make the commercialization of this product a challenge. The identification and development of new products with antifungal action, such as essential oils (EOs), is a promising alternative. Therefore, the present study addresses: the in vitro antifungal activity of three essential oils, Eucalyptus staigeriana, Lippia sidoides and Pimenta pseudocaryophyllus; the chemical composition and the mode of action of the EO with the highest potential and its in vivo activity when associated with carboxymethylcellulose (CMC), as well as its effects on the sensory quality and postharvest of strawberries. In chapters two, three and four, the studies were directed to Colletotrichum acutatum, Rhizopus stolonifer and Botrytis cinerea, respectively. In the last chapter, the antifungal activity of a commercial, alternative formulation with low toxicity was evaluated against the same three pathogens. Among the EOs evaluated, the one from L. sidoides presented the best antifungal activity, with the predominance of thymol in its composition, causing morphological alterations in the pathogens. in vivo, the strawberries treated with CMC associated to the EO presented a reduction in the severity of the diseases and improvements in the physicochemical properties with little alteration in the sensory quality of the fruit. The commercial formulation also presented a potential for the control, in vitro and in vivo, of the main postharvest strawberry-deteriorating fungi.
publishDate 2019
dc.date.none.fl_str_mv 2019-02-05
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.teses.usp.br/teses/disponiveis/11/11141/tde-24052019-145152/
url http://www.teses.usp.br/teses/disponiveis/11/11141/tde-24052019-145152/
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv
dc.rights.driver.fl_str_mv Liberar o conteúdo para acesso público.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Liberar o conteúdo para acesso público.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv
dc.publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
dc.source.none.fl_str_mv
reponame:Biblioteca Digital de Teses e Dissertações da USP
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
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institution USP
reponame_str Biblioteca Digital de Teses e Dissertações da USP
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repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)
repository.mail.fl_str_mv virginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.br
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