Study on leaf-cutting ants: from laboratory maintenance to applied personality studies with behavioral and molecular approaches

Detalhes bibliográficos
Autor(a) principal: Silva, Janiele Pereira da
Data de Publicação: 2022
Tipo de documento: Tese
Idioma: eng
Título da fonte: Biblioteca Digital de Teses e Dissertações da USP
Texto Completo: https://www.teses.usp.br/teses/disponiveis/47/47132/tde-06092022-084659/
Resumo: Atta sexdens, popularly known as leaf-cutting ant, is a species of biological and economic importance. Although these leaf-cutting ants have been studied for decades, there are still unanswered questions. An alternative that helps the development of research difficult to be executed in field is the maintenance of colonies under controlled conditions, such as in a laboratory. Tests realized in a laboratory environment can assess different lines of research, such as behavior and genetics. Considering the advantages of maintaining colonies of leaf-cutting ants in the laboratory, this thesis addresses the relationship between the species and research, covering the initial stages of creation until its application in studies in different areas. The thesis is divided into chapters. Chapter 1 introduces the topics explored and outlines of the thesis. Chapter 2 is composed by the protocol for collecting and maintaining leaf-cutting ants in the laboratory, followed by results exemplifying research and emphasizing the educational importance of ants. In Chapter 3, behavioral tests were performed to assess the existence of personality and behavioral syndrome in leaf-cutting ants. The results indicate that both exist in multilevels (colony and caste), in addition to some traits being related to worker size and subcastes. Chapter 4 (under development) investigated the reproduction system and genetic diversity associated with the personality of leaf-cutting ants. Preliminary results indicate that polyandry level is higher than literature recorded, but increasing the genetic variability of the colony. And low paternity skew within colonies gives evidence of uniform use of sperm. But only with the conclusion of the analysis will it be possible to clarify the genetic relationship within the colony with the personality of the workers
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spelling Study on leaf-cutting ants: from laboratory maintenance to applied personality studies with behavioral and molecular approachesEstudo de formigas-cortadeiras: da criação em laboratório aos estudos aplicados de personalidade com abordagens comportamentais e molecularesAtta sexdensAtta sexdensbehaviorbehavior syndromecomportamentogeneticgenéticaprotocolprotocolosíndrome comportamentalAtta sexdens, popularly known as leaf-cutting ant, is a species of biological and economic importance. Although these leaf-cutting ants have been studied for decades, there are still unanswered questions. An alternative that helps the development of research difficult to be executed in field is the maintenance of colonies under controlled conditions, such as in a laboratory. Tests realized in a laboratory environment can assess different lines of research, such as behavior and genetics. Considering the advantages of maintaining colonies of leaf-cutting ants in the laboratory, this thesis addresses the relationship between the species and research, covering the initial stages of creation until its application in studies in different areas. The thesis is divided into chapters. Chapter 1 introduces the topics explored and outlines of the thesis. Chapter 2 is composed by the protocol for collecting and maintaining leaf-cutting ants in the laboratory, followed by results exemplifying research and emphasizing the educational importance of ants. In Chapter 3, behavioral tests were performed to assess the existence of personality and behavioral syndrome in leaf-cutting ants. The results indicate that both exist in multilevels (colony and caste), in addition to some traits being related to worker size and subcastes. Chapter 4 (under development) investigated the reproduction system and genetic diversity associated with the personality of leaf-cutting ants. Preliminary results indicate that polyandry level is higher than literature recorded, but increasing the genetic variability of the colony. And low paternity skew within colonies gives evidence of uniform use of sperm. But only with the conclusion of the analysis will it be possible to clarify the genetic relationship within the colony with the personality of the workersA Atta sexdens, popularmente conhecida como formiga cortadeira, é uma espécie de importância biológica e econômica. Embora essas formigas-cortadeiras tenham sido estudadas por décadas, ainda existem perguntas sem resposta. Uma alternativa que auxilia o desenvolvimento de pesquisas difíceis de serem realizadas em campo, é a criação de colônias em condições controladas, como em laboratório. Testes realizados em ambiente laboratorial podem avaliar diferentes linhas de pesquisa, como comportamento e genética. Considerando as vantagens de manter colônias de formigas-cortadeiras em laboratório, essa tese aborda a relação da espécie com a pesquisa, abordando as etapas iniciais de criação até a aplicação em estudos de diversas áreas. A tese está dividida em capítulos. O Capítulo 1 introduz os temas abordados e linhas gerais da tese. O Capítulo 2 é composto pelo protocolo de coleta e manutenção de formigas-cortadeiras em laboratório, seguido de resultados exemplificando pesquisas e salientando a importância educacional das formigas. No Capítulo 3 foram realizados testes comportamentais para avaliar a existência de personalidade e síndrome comportamental em formigas-cortadeiras. Os resultados indicam que ambos existem em multiníveis (colônia e casta), além de alguns traços estarem relacionados com o tamanho das operárias e as subcastas. O capítulo 4 (em desenvolvimento) investigou o sistema de reprodução e diversidade genética associado a personalidade das formigas-cortadeiras. Resultados preliminares indicam que poliandria na espécie é maior do que os registros na literatura, aumentando a variabilidade genética da colônia. E o baixo desvio de paternidade dentro das colônias evidencia o uso uniforme de esperma. Mas apenas com a conclusão das análises será possível esclarecer a relação genética dentro colônia com a personalidade das operáriasBiblioteca Digitais de Teses e Dissertações da USPBueno, Odair CorreaResende, Briseida Dogo deSilva, Janiele Pereira da2022-06-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttps://www.teses.usp.br/teses/disponiveis/47/47132/tde-06092022-084659/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPLiberar o conteúdo para acesso público.info:eu-repo/semantics/openAccesseng2022-09-12T20:08:02Zoai:teses.usp.br:tde-06092022-084659Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212022-09-12T20:08:02Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Study on leaf-cutting ants: from laboratory maintenance to applied personality studies with behavioral and molecular approaches
Estudo de formigas-cortadeiras: da criação em laboratório aos estudos aplicados de personalidade com abordagens comportamentais e moleculares
title Study on leaf-cutting ants: from laboratory maintenance to applied personality studies with behavioral and molecular approaches
spellingShingle Study on leaf-cutting ants: from laboratory maintenance to applied personality studies with behavioral and molecular approaches
Silva, Janiele Pereira da
Atta sexdens
Atta sexdens
behavior
behavior syndrome
comportamento
genetic
genética
protocol
protocolo
síndrome comportamental
title_short Study on leaf-cutting ants: from laboratory maintenance to applied personality studies with behavioral and molecular approaches
title_full Study on leaf-cutting ants: from laboratory maintenance to applied personality studies with behavioral and molecular approaches
title_fullStr Study on leaf-cutting ants: from laboratory maintenance to applied personality studies with behavioral and molecular approaches
title_full_unstemmed Study on leaf-cutting ants: from laboratory maintenance to applied personality studies with behavioral and molecular approaches
title_sort Study on leaf-cutting ants: from laboratory maintenance to applied personality studies with behavioral and molecular approaches
author Silva, Janiele Pereira da
author_facet Silva, Janiele Pereira da
author_role author
dc.contributor.none.fl_str_mv Bueno, Odair Correa
Resende, Briseida Dogo de
dc.contributor.author.fl_str_mv Silva, Janiele Pereira da
dc.subject.por.fl_str_mv Atta sexdens
Atta sexdens
behavior
behavior syndrome
comportamento
genetic
genética
protocol
protocolo
síndrome comportamental
topic Atta sexdens
Atta sexdens
behavior
behavior syndrome
comportamento
genetic
genética
protocol
protocolo
síndrome comportamental
description Atta sexdens, popularly known as leaf-cutting ant, is a species of biological and economic importance. Although these leaf-cutting ants have been studied for decades, there are still unanswered questions. An alternative that helps the development of research difficult to be executed in field is the maintenance of colonies under controlled conditions, such as in a laboratory. Tests realized in a laboratory environment can assess different lines of research, such as behavior and genetics. Considering the advantages of maintaining colonies of leaf-cutting ants in the laboratory, this thesis addresses the relationship between the species and research, covering the initial stages of creation until its application in studies in different areas. The thesis is divided into chapters. Chapter 1 introduces the topics explored and outlines of the thesis. Chapter 2 is composed by the protocol for collecting and maintaining leaf-cutting ants in the laboratory, followed by results exemplifying research and emphasizing the educational importance of ants. In Chapter 3, behavioral tests were performed to assess the existence of personality and behavioral syndrome in leaf-cutting ants. The results indicate that both exist in multilevels (colony and caste), in addition to some traits being related to worker size and subcastes. Chapter 4 (under development) investigated the reproduction system and genetic diversity associated with the personality of leaf-cutting ants. Preliminary results indicate that polyandry level is higher than literature recorded, but increasing the genetic variability of the colony. And low paternity skew within colonies gives evidence of uniform use of sperm. But only with the conclusion of the analysis will it be possible to clarify the genetic relationship within the colony with the personality of the workers
publishDate 2022
dc.date.none.fl_str_mv 2022-06-30
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.teses.usp.br/teses/disponiveis/47/47132/tde-06092022-084659/
url https://www.teses.usp.br/teses/disponiveis/47/47132/tde-06092022-084659/
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv
dc.rights.driver.fl_str_mv Liberar o conteúdo para acesso público.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Liberar o conteúdo para acesso público.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv
dc.publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
publisher.none.fl_str_mv Biblioteca Digitais de Teses e Dissertações da USP
dc.source.none.fl_str_mv
reponame:Biblioteca Digital de Teses e Dissertações da USP
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Biblioteca Digital de Teses e Dissertações da USP
collection Biblioteca Digital de Teses e Dissertações da USP
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)
repository.mail.fl_str_mv virginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.br
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