Experimental approaches to study the responses to Al stress in cultivated crops
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Tese |
Idioma: | eng |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da USP |
Texto Completo: | http://www.teses.usp.br/teses/disponiveis/11/11151/tde-10062014-105216/ |
Resumo: | This thesis was performed in the scope of International Graduation Program in which Universidade de São Paulo and The Ohio State University are inserted. In the first year of Ph.D. work we aimed to understand the effect of aluminum (Al) in root growth of Eucalyptus spp. and the relationship with biochemical parameters and transcriptional responses since there is little information about these responses for tree species. Aluminum is found naturally in the Earth crust, but under acidic conditions Al3+ ion becomes available and toxic to the plants. United States and Brazil possess large areas with Al toxicity and great area of forests growing under these conditions. In order to perform the transcriptional analysis, Eucalyptus RNA was transported to United States, however, the method of transportation was not efficient to guarantee RNA integrity. Therefore we changed our objectives and we performed new experiments using maize as biological system because this species has economical importance to U.S. and Brazil. Furthermore, substantial area of this crop is growing in soils under these conditions. In these experiments, we aimed to verify if pre-treating maize seeds before germination could be efficient to induce Al-tolerance in Al-sensitive genotypes. This thesis was organized in order to build a story based on the sequences of the experiments. In general, we obtained interesting results for Al-sensitive maize that presented reduction in lignin content and gene expression in shoots, also for Al-tolerant variety that present increase in growth, productivity and improvement of nutritional quality of the kernels. |
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Experimental approaches to study the responses to Al stress in cultivated cropsAbordagens experimentais para o estudo das respostas ao estresse por Al em plantas cultivadasAcidic soilAlumínio trocávelEstresse oxidativoExchangeable aluminumLigninLigninaOxidative stressProductivityProdutividadeSolo ácidoThis thesis was performed in the scope of International Graduation Program in which Universidade de São Paulo and The Ohio State University are inserted. In the first year of Ph.D. work we aimed to understand the effect of aluminum (Al) in root growth of Eucalyptus spp. and the relationship with biochemical parameters and transcriptional responses since there is little information about these responses for tree species. Aluminum is found naturally in the Earth crust, but under acidic conditions Al3+ ion becomes available and toxic to the plants. United States and Brazil possess large areas with Al toxicity and great area of forests growing under these conditions. In order to perform the transcriptional analysis, Eucalyptus RNA was transported to United States, however, the method of transportation was not efficient to guarantee RNA integrity. Therefore we changed our objectives and we performed new experiments using maize as biological system because this species has economical importance to U.S. and Brazil. Furthermore, substantial area of this crop is growing in soils under these conditions. In these experiments, we aimed to verify if pre-treating maize seeds before germination could be efficient to induce Al-tolerance in Al-sensitive genotypes. This thesis was organized in order to build a story based on the sequences of the experiments. In general, we obtained interesting results for Al-sensitive maize that presented reduction in lignin content and gene expression in shoots, also for Al-tolerant variety that present increase in growth, productivity and improvement of nutritional quality of the kernels.Esta tese foi realizada no âmbito do Programa Internacional de Pós-Graduação em que a Universidade de São Paulo e The Ohio State University estão inseridos. No primeiro ano do trabalho de doutorado, o objetivo foi de compreender o efeito do alumínio (Al) no crescimento de raízes de Eucalyptus spp. e a relação com parâmetros bioquímicos e respostas de transcrição já que há pouca informação sobre estas respostas para espécies arbóreas. O alumínio é encontrado naturalmente na crosta terrestre, porém sob condições ácidas o íon Al3+ torna-se disponível e tóxico para as plantas. Os Estados Unidos e o Brasil possuem grandes áreas com Al tóxico e grandes áreas de florestas crescem sob estas condições. A fim de executar a análise transcricional, o RNA dos materiais de eucalipto foi transportado para os Estados Unidos, entretanto, o método de transporte não foi eficiente para garantir a integridade do RNA. Assim sendo, mudamos nossos objetivos e realizamos novos experimentos utilizando milho como sistema biológico, porque esta espécie tem importância econômica para os EUA e também para o Brasil. Ademais, substanciais áreas de cultivo do milho se encontram em solos sob estas condições. Nos novos experimentos realizados, o objetivo foi de verificar se o pré-tratamento de sementes de milho antes da germinação poderia ser eficiente para induzir tolerância ao alumínio em genótipos Al-sensíveis. Esta tese foi organizada a fim de construir uma história com base nas sequencias dos experimentos. Em geral, resultados interessantes foram obtidos para a linhagem de milho sensível ao Al que apresentou redução no teor de lignina e na sua expressão gênica em parte aérea, também para a variedade tolerante ao Al que apresentou aumento no crescimento, produtividade e melhoria da qualidade nutricional dos grãos.Biblioteca Digitais de Teses e Dissertações da USPAzevedo, Ricardo Antunes deAlcantara, Berenice Kussumoto de2014-05-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisapplication/pdfhttp://www.teses.usp.br/teses/disponiveis/11/11151/tde-10062014-105216/reponame:Biblioteca Digital de Teses e Dissertações da USPinstname:Universidade de São Paulo (USP)instacron:USPLiberar o conteúdo para acesso público.info:eu-repo/semantics/openAccesseng2016-07-28T16:11:49Zoai:teses.usp.br:tde-10062014-105216Biblioteca Digital de Teses e Dissertaçõeshttp://www.teses.usp.br/PUBhttp://www.teses.usp.br/cgi-bin/mtd2br.plvirginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.bropendoar:27212016-07-28T16:11:49Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Experimental approaches to study the responses to Al stress in cultivated crops Abordagens experimentais para o estudo das respostas ao estresse por Al em plantas cultivadas |
title |
Experimental approaches to study the responses to Al stress in cultivated crops |
spellingShingle |
Experimental approaches to study the responses to Al stress in cultivated crops Alcantara, Berenice Kussumoto de Acidic soil Alumínio trocável Estresse oxidativo Exchangeable aluminum Lignin Lignina Oxidative stress Productivity Produtividade Solo ácido |
title_short |
Experimental approaches to study the responses to Al stress in cultivated crops |
title_full |
Experimental approaches to study the responses to Al stress in cultivated crops |
title_fullStr |
Experimental approaches to study the responses to Al stress in cultivated crops |
title_full_unstemmed |
Experimental approaches to study the responses to Al stress in cultivated crops |
title_sort |
Experimental approaches to study the responses to Al stress in cultivated crops |
author |
Alcantara, Berenice Kussumoto de |
author_facet |
Alcantara, Berenice Kussumoto de |
author_role |
author |
dc.contributor.none.fl_str_mv |
Azevedo, Ricardo Antunes de |
dc.contributor.author.fl_str_mv |
Alcantara, Berenice Kussumoto de |
dc.subject.por.fl_str_mv |
Acidic soil Alumínio trocável Estresse oxidativo Exchangeable aluminum Lignin Lignina Oxidative stress Productivity Produtividade Solo ácido |
topic |
Acidic soil Alumínio trocável Estresse oxidativo Exchangeable aluminum Lignin Lignina Oxidative stress Productivity Produtividade Solo ácido |
description |
This thesis was performed in the scope of International Graduation Program in which Universidade de São Paulo and The Ohio State University are inserted. In the first year of Ph.D. work we aimed to understand the effect of aluminum (Al) in root growth of Eucalyptus spp. and the relationship with biochemical parameters and transcriptional responses since there is little information about these responses for tree species. Aluminum is found naturally in the Earth crust, but under acidic conditions Al3+ ion becomes available and toxic to the plants. United States and Brazil possess large areas with Al toxicity and great area of forests growing under these conditions. In order to perform the transcriptional analysis, Eucalyptus RNA was transported to United States, however, the method of transportation was not efficient to guarantee RNA integrity. Therefore we changed our objectives and we performed new experiments using maize as biological system because this species has economical importance to U.S. and Brazil. Furthermore, substantial area of this crop is growing in soils under these conditions. In these experiments, we aimed to verify if pre-treating maize seeds before germination could be efficient to induce Al-tolerance in Al-sensitive genotypes. This thesis was organized in order to build a story based on the sequences of the experiments. In general, we obtained interesting results for Al-sensitive maize that presented reduction in lignin content and gene expression in shoots, also for Al-tolerant variety that present increase in growth, productivity and improvement of nutritional quality of the kernels. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-05-21 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.teses.usp.br/teses/disponiveis/11/11151/tde-10062014-105216/ |
url |
http://www.teses.usp.br/teses/disponiveis/11/11151/tde-10062014-105216/ |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
|
dc.rights.driver.fl_str_mv |
Liberar o conteúdo para acesso público. info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Liberar o conteúdo para acesso público. |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
|
dc.publisher.none.fl_str_mv |
Biblioteca Digitais de Teses e Dissertações da USP |
publisher.none.fl_str_mv |
Biblioteca Digitais de Teses e Dissertações da USP |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da USP instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Biblioteca Digital de Teses e Dissertações da USP |
collection |
Biblioteca Digital de Teses e Dissertações da USP |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da USP - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
virginia@if.usp.br|| atendimento@aguia.usp.br||virginia@if.usp.br |
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1815256912973791232 |