Role of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceus

Detalhes bibliográficos
Autor(a) principal: Portes, Ciro da Silva
Data de Publicação: 2013
Outros Autores: Oliveira, Andriélen Virke de, Simer, Patrícia, Machado-Lunkes, Alessandra, Coelho, Alexandre Rodrigo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UTFPR (da Universidade Tecnológica Federal do Paraná (RIUT))
Texto Completo: http://repositorio.utfpr.edu.br/jspui/handle/1/29978
https://doi.org/10.1590/S1516-89132013000400012
Resumo: This work evaluated the antagonism of killer positive yeast strains (isolated from 11 samples of different frozen fruit pulps) against the strains of Penicillium expansum and Aspergillus ochraceus. Of the total 41 killer yeasts tested in YM agar, 19 showed antibiosis against P. expansum and A. ochraceus, with inhibition zone ranging from 10 to 18 mm and 10 to 19 mm, respectively. In the following step, the extracellular activity of Kluyveromyces sp. FP4(13) was tested performing the assay in YM broth. The antifungal activity of Kluyveromyces sp. FP4(13) cell-free culture supernatant (25ºC/96 h) was more effective against the conidia germination, showing inhibition rates of 93.33 and 86.44% for P. expansum and A. ochraceus, respectively. The micelial growth inhibition was 28.45 and 21.0%, respectively. The antagonism showed by the selected yeasts could be used as a promising alternative tool to reduce and control the postharvest fungal spoilage of the fruits. However, further studies should be carried out in order to better elucidate the role of innocuous characters in antagonistic microorganisms, as well as the purification and characterization of new killer toxins.
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spelling 2022-10-21T21:27:11Z2022-10-21T21:27:11Z2013PORTES, Ciro da Silva et al. Role of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceus. Brazilian Archives of Biology and Technology, Curitiba, v. 56, n. 4, p. 619-627, July/Aug 2013. Disponível em: https://www.scielo.br/j/babt/a/g8cXpMH5jNtMzGPrSWD4Qdq/?lang=en. Acesso em: 21 out. 2022.1678-4324http://repositorio.utfpr.edu.br/jspui/handle/1/29978https://doi.org/10.1590/S1516-89132013000400012This work evaluated the antagonism of killer positive yeast strains (isolated from 11 samples of different frozen fruit pulps) against the strains of Penicillium expansum and Aspergillus ochraceus. Of the total 41 killer yeasts tested in YM agar, 19 showed antibiosis against P. expansum and A. ochraceus, with inhibition zone ranging from 10 to 18 mm and 10 to 19 mm, respectively. In the following step, the extracellular activity of Kluyveromyces sp. FP4(13) was tested performing the assay in YM broth. The antifungal activity of Kluyveromyces sp. FP4(13) cell-free culture supernatant (25ºC/96 h) was more effective against the conidia germination, showing inhibition rates of 93.33 and 86.44% for P. expansum and A. ochraceus, respectively. The micelial growth inhibition was 28.45 and 21.0%, respectively. The antagonism showed by the selected yeasts could be used as a promising alternative tool to reduce and control the postharvest fungal spoilage of the fruits. However, further studies should be carried out in order to better elucidate the role of innocuous characters in antagonistic microorganisms, as well as the purification and characterization of new killer toxins.engBrazilian Archives of Biology and Technologyhttps://www.scielo.br/j/babt/a/g8cXpMH5jNtMzGPrSWD4Qdq/?lang=enhttps://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessCNPQ::CIENCIAS BIOLOGICASLeveduras (Fungos)Mofo (Botânica)AntibioseYeast fungiMolds (Fungi)AntibiosisRole of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLondrinaBrasil564Portes, Ciro da SilvaOliveira, Andriélen Virke deSimer, PatríciaMachado-Lunkes, AlessandraCoelho, Alexandre Rodrigoreponame:Repositório Institucional da UTFPR (da Universidade Tecnológica Federal do Paraná (RIUT))instname:Universidade Tecnológica Federal do Paraná (UTFPR)instacron:UTFPRORIGINALinhibitoryactivitybiocontrolyeasts.pdfinhibitoryactivitybiocontrolyeasts.pdfapplication/pdf175382http://repositorio.utfpr.edu.br:8080/jspui/bitstream/1/29978/1/inhibitoryactivitybiocontrolyeasts.pdffaff6c3a8497e7d148aeb17c52208fbcMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914http://repositorio.utfpr.edu.br:8080/jspui/bitstream/1/29978/2/license_rdf24013099e9e6abb1575dc6ce0855efd5MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81290http://repositorio.utfpr.edu.br:8080/jspui/bitstream/1/29978/3/license.txtb9d82215ab23456fa2d8b49c5df1b95bMD53TEXTinhibitoryactivitybiocontrolyeasts.pdf.txtinhibitoryactivitybiocontrolyeasts.pdf.txtExtracted texttext/plain32072http://repositorio.utfpr.edu.br:8080/jspui/bitstream/1/29978/4/inhibitoryactivitybiocontrolyeasts.pdf.txt8e6fad81bbbd0634cb71fdbf1bca43b5MD54THUMBNAILinhibitoryactivitybiocontrolyeasts.pdf.jpginhibitoryactivitybiocontrolyeasts.pdf.jpgGenerated Thumbnailimage/jpeg1666http://repositorio.utfpr.edu.br:8080/jspui/bitstream/1/29978/5/inhibitoryactivitybiocontrolyeasts.pdf.jpgdf1110f2eb1c953ab657a5fd94791364MD551/299782022-10-22 03:07:02.941oai:repositorio.utfpr.edu.br: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ório de PublicaçõesPUBhttp://repositorio.utfpr.edu.br:8080/oai/requestopendoar:2022-10-22T06:07:02Repositório Institucional da UTFPR (da Universidade Tecnológica Federal do Paraná (RIUT)) - Universidade Tecnológica Federal do Paraná (UTFPR)false
dc.title.pt_BR.fl_str_mv Role of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceus
title Role of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceus
spellingShingle Role of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceus
Portes, Ciro da Silva
CNPQ::CIENCIAS BIOLOGICAS
Leveduras (Fungos)
Mofo (Botânica)
Antibiose
Yeast fungi
Molds (Fungi)
Antibiosis
title_short Role of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceus
title_full Role of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceus
title_fullStr Role of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceus
title_full_unstemmed Role of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceus
title_sort Role of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceus
author Portes, Ciro da Silva
author_facet Portes, Ciro da Silva
Oliveira, Andriélen Virke de
Simer, Patrícia
Machado-Lunkes, Alessandra
Coelho, Alexandre Rodrigo
author_role author
author2 Oliveira, Andriélen Virke de
Simer, Patrícia
Machado-Lunkes, Alessandra
Coelho, Alexandre Rodrigo
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Portes, Ciro da Silva
Oliveira, Andriélen Virke de
Simer, Patrícia
Machado-Lunkes, Alessandra
Coelho, Alexandre Rodrigo
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS
topic CNPQ::CIENCIAS BIOLOGICAS
Leveduras (Fungos)
Mofo (Botânica)
Antibiose
Yeast fungi
Molds (Fungi)
Antibiosis
dc.subject.por.fl_str_mv Leveduras (Fungos)
Mofo (Botânica)
Antibiose
Yeast fungi
Molds (Fungi)
Antibiosis
description This work evaluated the antagonism of killer positive yeast strains (isolated from 11 samples of different frozen fruit pulps) against the strains of Penicillium expansum and Aspergillus ochraceus. Of the total 41 killer yeasts tested in YM agar, 19 showed antibiosis against P. expansum and A. ochraceus, with inhibition zone ranging from 10 to 18 mm and 10 to 19 mm, respectively. In the following step, the extracellular activity of Kluyveromyces sp. FP4(13) was tested performing the assay in YM broth. The antifungal activity of Kluyveromyces sp. FP4(13) cell-free culture supernatant (25ºC/96 h) was more effective against the conidia germination, showing inhibition rates of 93.33 and 86.44% for P. expansum and A. ochraceus, respectively. The micelial growth inhibition was 28.45 and 21.0%, respectively. The antagonism showed by the selected yeasts could be used as a promising alternative tool to reduce and control the postharvest fungal spoilage of the fruits. However, further studies should be carried out in order to better elucidate the role of innocuous characters in antagonistic microorganisms, as well as the purification and characterization of new killer toxins.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2022-10-21T21:27:11Z
dc.date.available.fl_str_mv 2022-10-21T21:27:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv PORTES, Ciro da Silva et al. Role of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceus. Brazilian Archives of Biology and Technology, Curitiba, v. 56, n. 4, p. 619-627, July/Aug 2013. Disponível em: https://www.scielo.br/j/babt/a/g8cXpMH5jNtMzGPrSWD4Qdq/?lang=en. Acesso em: 21 out. 2022.
dc.identifier.uri.fl_str_mv http://repositorio.utfpr.edu.br/jspui/handle/1/29978
dc.identifier.issn.pt_BR.fl_str_mv 1678-4324
dc.identifier.doi.pt_BR.fl_str_mv https://doi.org/10.1590/S1516-89132013000400012
identifier_str_mv PORTES, Ciro da Silva et al. Role of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceus. Brazilian Archives of Biology and Technology, Curitiba, v. 56, n. 4, p. 619-627, July/Aug 2013. Disponível em: https://www.scielo.br/j/babt/a/g8cXpMH5jNtMzGPrSWD4Qdq/?lang=en. Acesso em: 21 out. 2022.
1678-4324
url http://repositorio.utfpr.edu.br/jspui/handle/1/29978
https://doi.org/10.1590/S1516-89132013000400012
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language eng
dc.relation.ispartof.pt_BR.fl_str_mv Brazilian Archives of Biology and Technology
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