Role of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceus
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UTFPR (da Universidade Tecnológica Federal do Paraná (RIUT)) |
Texto Completo: | http://repositorio.utfpr.edu.br/jspui/handle/1/29978 https://doi.org/10.1590/S1516-89132013000400012 |
Resumo: | This work evaluated the antagonism of killer positive yeast strains (isolated from 11 samples of different frozen fruit pulps) against the strains of Penicillium expansum and Aspergillus ochraceus. Of the total 41 killer yeasts tested in YM agar, 19 showed antibiosis against P. expansum and A. ochraceus, with inhibition zone ranging from 10 to 18 mm and 10 to 19 mm, respectively. In the following step, the extracellular activity of Kluyveromyces sp. FP4(13) was tested performing the assay in YM broth. The antifungal activity of Kluyveromyces sp. FP4(13) cell-free culture supernatant (25ºC/96 h) was more effective against the conidia germination, showing inhibition rates of 93.33 and 86.44% for P. expansum and A. ochraceus, respectively. The micelial growth inhibition was 28.45 and 21.0%, respectively. The antagonism showed by the selected yeasts could be used as a promising alternative tool to reduce and control the postharvest fungal spoilage of the fruits. However, further studies should be carried out in order to better elucidate the role of innocuous characters in antagonistic microorganisms, as well as the purification and characterization of new killer toxins. |
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2022-10-21T21:27:11Z2022-10-21T21:27:11Z2013PORTES, Ciro da Silva et al. Role of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceus. Brazilian Archives of Biology and Technology, Curitiba, v. 56, n. 4, p. 619-627, July/Aug 2013. Disponível em: https://www.scielo.br/j/babt/a/g8cXpMH5jNtMzGPrSWD4Qdq/?lang=en. Acesso em: 21 out. 2022.1678-4324http://repositorio.utfpr.edu.br/jspui/handle/1/29978https://doi.org/10.1590/S1516-89132013000400012This work evaluated the antagonism of killer positive yeast strains (isolated from 11 samples of different frozen fruit pulps) against the strains of Penicillium expansum and Aspergillus ochraceus. Of the total 41 killer yeasts tested in YM agar, 19 showed antibiosis against P. expansum and A. ochraceus, with inhibition zone ranging from 10 to 18 mm and 10 to 19 mm, respectively. In the following step, the extracellular activity of Kluyveromyces sp. FP4(13) was tested performing the assay in YM broth. The antifungal activity of Kluyveromyces sp. FP4(13) cell-free culture supernatant (25ºC/96 h) was more effective against the conidia germination, showing inhibition rates of 93.33 and 86.44% for P. expansum and A. ochraceus, respectively. The micelial growth inhibition was 28.45 and 21.0%, respectively. The antagonism showed by the selected yeasts could be used as a promising alternative tool to reduce and control the postharvest fungal spoilage of the fruits. However, further studies should be carried out in order to better elucidate the role of innocuous characters in antagonistic microorganisms, as well as the purification and characterization of new killer toxins.engBrazilian Archives of Biology and Technologyhttps://www.scielo.br/j/babt/a/g8cXpMH5jNtMzGPrSWD4Qdq/?lang=enhttps://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessCNPQ::CIENCIAS BIOLOGICASLeveduras (Fungos)Mofo (Botânica)AntibioseYeast fungiMolds (Fungi)AntibiosisRole of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLondrinaBrasil564Portes, Ciro da SilvaOliveira, Andriélen Virke deSimer, PatríciaMachado-Lunkes, AlessandraCoelho, Alexandre Rodrigoreponame:Repositório Institucional da UTFPR (da Universidade Tecnológica Federal do Paraná (RIUT))instname:Universidade Tecnológica Federal do Paraná (UTFPR)instacron:UTFPRORIGINALinhibitoryactivitybiocontrolyeasts.pdfinhibitoryactivitybiocontrolyeasts.pdfapplication/pdf175382http://repositorio.utfpr.edu.br:8080/jspui/bitstream/1/29978/1/inhibitoryactivitybiocontrolyeasts.pdffaff6c3a8497e7d148aeb17c52208fbcMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914http://repositorio.utfpr.edu.br:8080/jspui/bitstream/1/29978/2/license_rdf24013099e9e6abb1575dc6ce0855efd5MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81290http://repositorio.utfpr.edu.br:8080/jspui/bitstream/1/29978/3/license.txtb9d82215ab23456fa2d8b49c5df1b95bMD53TEXTinhibitoryactivitybiocontrolyeasts.pdf.txtinhibitoryactivitybiocontrolyeasts.pdf.txtExtracted texttext/plain32072http://repositorio.utfpr.edu.br:8080/jspui/bitstream/1/29978/4/inhibitoryactivitybiocontrolyeasts.pdf.txt8e6fad81bbbd0634cb71fdbf1bca43b5MD54THUMBNAILinhibitoryactivitybiocontrolyeasts.pdf.jpginhibitoryactivitybiocontrolyeasts.pdf.jpgGenerated Thumbnailimage/jpeg1666http://repositorio.utfpr.edu.br:8080/jspui/bitstream/1/29978/5/inhibitoryactivitybiocontrolyeasts.pdf.jpgdf1110f2eb1c953ab657a5fd94791364MD551/299782022-10-22 03:07:02.941oai:repositorio.utfpr.edu.br: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ório de PublicaçõesPUBhttp://repositorio.utfpr.edu.br:8080/oai/requestopendoar:2022-10-22T06:07:02Repositório Institucional da UTFPR (da Universidade Tecnológica Federal do Paraná (RIUT)) - Universidade Tecnológica Federal do Paraná (UTFPR)false |
dc.title.pt_BR.fl_str_mv |
Role of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceus |
title |
Role of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceus |
spellingShingle |
Role of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceus Portes, Ciro da Silva CNPQ::CIENCIAS BIOLOGICAS Leveduras (Fungos) Mofo (Botânica) Antibiose Yeast fungi Molds (Fungi) Antibiosis |
title_short |
Role of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceus |
title_full |
Role of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceus |
title_fullStr |
Role of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceus |
title_full_unstemmed |
Role of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceus |
title_sort |
Role of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceus |
author |
Portes, Ciro da Silva |
author_facet |
Portes, Ciro da Silva Oliveira, Andriélen Virke de Simer, Patrícia Machado-Lunkes, Alessandra Coelho, Alexandre Rodrigo |
author_role |
author |
author2 |
Oliveira, Andriélen Virke de Simer, Patrícia Machado-Lunkes, Alessandra Coelho, Alexandre Rodrigo |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Portes, Ciro da Silva Oliveira, Andriélen Virke de Simer, Patrícia Machado-Lunkes, Alessandra Coelho, Alexandre Rodrigo |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS |
topic |
CNPQ::CIENCIAS BIOLOGICAS Leveduras (Fungos) Mofo (Botânica) Antibiose Yeast fungi Molds (Fungi) Antibiosis |
dc.subject.por.fl_str_mv |
Leveduras (Fungos) Mofo (Botânica) Antibiose Yeast fungi Molds (Fungi) Antibiosis |
description |
This work evaluated the antagonism of killer positive yeast strains (isolated from 11 samples of different frozen fruit pulps) against the strains of Penicillium expansum and Aspergillus ochraceus. Of the total 41 killer yeasts tested in YM agar, 19 showed antibiosis against P. expansum and A. ochraceus, with inhibition zone ranging from 10 to 18 mm and 10 to 19 mm, respectively. In the following step, the extracellular activity of Kluyveromyces sp. FP4(13) was tested performing the assay in YM broth. The antifungal activity of Kluyveromyces sp. FP4(13) cell-free culture supernatant (25ºC/96 h) was more effective against the conidia germination, showing inhibition rates of 93.33 and 86.44% for P. expansum and A. ochraceus, respectively. The micelial growth inhibition was 28.45 and 21.0%, respectively. The antagonism showed by the selected yeasts could be used as a promising alternative tool to reduce and control the postharvest fungal spoilage of the fruits. However, further studies should be carried out in order to better elucidate the role of innocuous characters in antagonistic microorganisms, as well as the purification and characterization of new killer toxins. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013 |
dc.date.accessioned.fl_str_mv |
2022-10-21T21:27:11Z |
dc.date.available.fl_str_mv |
2022-10-21T21:27:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PORTES, Ciro da Silva et al. Role of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceus. Brazilian Archives of Biology and Technology, Curitiba, v. 56, n. 4, p. 619-627, July/Aug 2013. Disponível em: https://www.scielo.br/j/babt/a/g8cXpMH5jNtMzGPrSWD4Qdq/?lang=en. Acesso em: 21 out. 2022. |
dc.identifier.uri.fl_str_mv |
http://repositorio.utfpr.edu.br/jspui/handle/1/29978 |
dc.identifier.issn.pt_BR.fl_str_mv |
1678-4324 |
dc.identifier.doi.pt_BR.fl_str_mv |
https://doi.org/10.1590/S1516-89132013000400012 |
identifier_str_mv |
PORTES, Ciro da Silva et al. Role of killer factors in the inhibitory activity of bio-control yeasts against Penicillium expansum and Aspergillus ochraceus. Brazilian Archives of Biology and Technology, Curitiba, v. 56, n. 4, p. 619-627, July/Aug 2013. Disponível em: https://www.scielo.br/j/babt/a/g8cXpMH5jNtMzGPrSWD4Qdq/?lang=en. Acesso em: 21 out. 2022. 1678-4324 |
url |
http://repositorio.utfpr.edu.br/jspui/handle/1/29978 https://doi.org/10.1590/S1516-89132013000400012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian Archives of Biology and Technology |
dc.relation.uri.pt_BR.fl_str_mv |
https://www.scielo.br/j/babt/a/g8cXpMH5jNtMzGPrSWD4Qdq/?lang=en |
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https://creativecommons.org/licenses/by-nc/4.0/ info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by-nc/4.0/ |
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openAccess |
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Londrina |
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Brasil |
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Londrina |
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