Control of reducing sugars in sugarcane bioenergy industries by colorimetric methods: Is it always possible?
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista Brasileira de Tecnologia Agroindustrial |
Texto Completo: | https://periodicos.utfpr.edu.br/rbta/article/view/15265 |
Resumo: | Interferents influence analytical results of colorimetric methods. This work evaluated the level of analytical influence by metallic ions (K+, Ca2+ and Fe3+) on reducing sugars analysis. The difference on the reducing sugars (RS) content was analyzed by the 3,5-DNS, Somogyi-Nelson, Lane-Eynon and ion chromatography (IC) methods. The RS contents were compared to each other, using sugarcane juice samples. The same sample presented, generally, Somogyi-Nelson with higher contents, while 3,5-DNS lower. Therefore, mixed solutions with 1.5 g L-1 (proportion 1:1, v/v) of glucose and fructose were prepared with increasing doses of K+, Ca2+and Fe3+ ions (2.6; 5.2; 7.8; 10.4; 13.0 mmolc L-1). K+ underestimated the results of 3,5-DNS. Nevertheless, Ca2+ overestimated the 3,5-DNS and Lane-Eynon results. While, Fe3+ overestimated the RS content in 3,5-DNS and underestimated in Somogyi-Nelson. Regardless of the metallic ion added, ion chromatography was more stable and showed the most accurate and exact methodology. Lastly, the use of colorimetric methods can interfere with sugarcane process monitoring, especially fermentable yield. |
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Control of reducing sugars in sugarcane bioenergy industries by colorimetric methods: Is it always possible?Ciência e Tecnologia de AlimentosColorimetric methods; Cane juice analysis; Fructose; Glucose; Ion chromatographyInterferents influence analytical results of colorimetric methods. This work evaluated the level of analytical influence by metallic ions (K+, Ca2+ and Fe3+) on reducing sugars analysis. The difference on the reducing sugars (RS) content was analyzed by the 3,5-DNS, Somogyi-Nelson, Lane-Eynon and ion chromatography (IC) methods. The RS contents were compared to each other, using sugarcane juice samples. The same sample presented, generally, Somogyi-Nelson with higher contents, while 3,5-DNS lower. Therefore, mixed solutions with 1.5 g L-1 (proportion 1:1, v/v) of glucose and fructose were prepared with increasing doses of K+, Ca2+and Fe3+ ions (2.6; 5.2; 7.8; 10.4; 13.0 mmolc L-1). K+ underestimated the results of 3,5-DNS. Nevertheless, Ca2+ overestimated the 3,5-DNS and Lane-Eynon results. While, Fe3+ overestimated the RS content in 3,5-DNS and underestimated in Somogyi-Nelson. Regardless of the metallic ion added, ion chromatography was more stable and showed the most accurate and exact methodology. Lastly, the use of colorimetric methods can interfere with sugarcane process monitoring, especially fermentable yield.Universidade Tecnológica Federal do Paraná (UTFPR)Ogando, Felipe Iwagaki BragaSantos, Carlos BernardoNouer, Pedro AggioAlves, Marcelo EduardoAguiar, Claudio Lima2022-12-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.utfpr.edu.br/rbta/article/view/1526510.3895/rbta.v16n2.15265Revista Brasileira de Tecnologia Agroindustrial; v. 16, n. 2 (2022)1981-368610.3895/rbta.v16n2reponame:Revista Brasileira de Tecnologia Agroindustrialinstname:Universidade Tecnológica Federal do Paraná (UTFPR)instacron:UTFPRporhttps://periodicos.utfpr.edu.br/rbta/article/view/15265/9379Direitos autorais 2022 CC-BYhttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess2023-01-16T17:50:51Zoai:periodicos.utfpr:article/15265Revistahttps://periodicos.utfpr.edu.br/rbtaPUBhttps://periodicos.utfpr.edu.br/rbta/oai||rbta-pg@utfpr.edu.br1981-36861981-3686opendoar:2023-01-16T17:50:51Revista Brasileira de Tecnologia Agroindustrial - Universidade Tecnológica Federal do Paraná (UTFPR)false |
dc.title.none.fl_str_mv |
Control of reducing sugars in sugarcane bioenergy industries by colorimetric methods: Is it always possible? |
title |
Control of reducing sugars in sugarcane bioenergy industries by colorimetric methods: Is it always possible? |
spellingShingle |
Control of reducing sugars in sugarcane bioenergy industries by colorimetric methods: Is it always possible? Ogando, Felipe Iwagaki Braga Ciência e Tecnologia de Alimentos Colorimetric methods; Cane juice analysis; Fructose; Glucose; Ion chromatography |
title_short |
Control of reducing sugars in sugarcane bioenergy industries by colorimetric methods: Is it always possible? |
title_full |
Control of reducing sugars in sugarcane bioenergy industries by colorimetric methods: Is it always possible? |
title_fullStr |
Control of reducing sugars in sugarcane bioenergy industries by colorimetric methods: Is it always possible? |
title_full_unstemmed |
Control of reducing sugars in sugarcane bioenergy industries by colorimetric methods: Is it always possible? |
title_sort |
Control of reducing sugars in sugarcane bioenergy industries by colorimetric methods: Is it always possible? |
author |
Ogando, Felipe Iwagaki Braga |
author_facet |
Ogando, Felipe Iwagaki Braga Santos, Carlos Bernardo Nouer, Pedro Aggio Alves, Marcelo Eduardo Aguiar, Claudio Lima |
author_role |
author |
author2 |
Santos, Carlos Bernardo Nouer, Pedro Aggio Alves, Marcelo Eduardo Aguiar, Claudio Lima |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
|
dc.contributor.author.fl_str_mv |
Ogando, Felipe Iwagaki Braga Santos, Carlos Bernardo Nouer, Pedro Aggio Alves, Marcelo Eduardo Aguiar, Claudio Lima |
dc.subject.por.fl_str_mv |
Ciência e Tecnologia de Alimentos Colorimetric methods; Cane juice analysis; Fructose; Glucose; Ion chromatography |
topic |
Ciência e Tecnologia de Alimentos Colorimetric methods; Cane juice analysis; Fructose; Glucose; Ion chromatography |
description |
Interferents influence analytical results of colorimetric methods. This work evaluated the level of analytical influence by metallic ions (K+, Ca2+ and Fe3+) on reducing sugars analysis. The difference on the reducing sugars (RS) content was analyzed by the 3,5-DNS, Somogyi-Nelson, Lane-Eynon and ion chromatography (IC) methods. The RS contents were compared to each other, using sugarcane juice samples. The same sample presented, generally, Somogyi-Nelson with higher contents, while 3,5-DNS lower. Therefore, mixed solutions with 1.5 g L-1 (proportion 1:1, v/v) of glucose and fructose were prepared with increasing doses of K+, Ca2+and Fe3+ ions (2.6; 5.2; 7.8; 10.4; 13.0 mmolc L-1). K+ underestimated the results of 3,5-DNS. Nevertheless, Ca2+ overestimated the 3,5-DNS and Lane-Eynon results. While, Fe3+ overestimated the RS content in 3,5-DNS and underestimated in Somogyi-Nelson. Regardless of the metallic ion added, ion chromatography was more stable and showed the most accurate and exact methodology. Lastly, the use of colorimetric methods can interfere with sugarcane process monitoring, especially fermentable yield. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-29 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.utfpr.edu.br/rbta/article/view/15265 10.3895/rbta.v16n2.15265 |
url |
https://periodicos.utfpr.edu.br/rbta/article/view/15265 |
identifier_str_mv |
10.3895/rbta.v16n2.15265 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://periodicos.utfpr.edu.br/rbta/article/view/15265/9379 |
dc.rights.driver.fl_str_mv |
Direitos autorais 2022 CC-BY http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Direitos autorais 2022 CC-BY http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Tecnológica Federal do Paraná (UTFPR) |
publisher.none.fl_str_mv |
Universidade Tecnológica Federal do Paraná (UTFPR) |
dc.source.none.fl_str_mv |
Revista Brasileira de Tecnologia Agroindustrial; v. 16, n. 2 (2022) 1981-3686 10.3895/rbta.v16n2 reponame:Revista Brasileira de Tecnologia Agroindustrial instname:Universidade Tecnológica Federal do Paraná (UTFPR) instacron:UTFPR |
instname_str |
Universidade Tecnológica Federal do Paraná (UTFPR) |
instacron_str |
UTFPR |
institution |
UTFPR |
reponame_str |
Revista Brasileira de Tecnologia Agroindustrial |
collection |
Revista Brasileira de Tecnologia Agroindustrial |
repository.name.fl_str_mv |
Revista Brasileira de Tecnologia Agroindustrial - Universidade Tecnológica Federal do Paraná (UTFPR) |
repository.mail.fl_str_mv |
||rbta-pg@utfpr.edu.br |
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1809282203211792384 |