IMMUNOSENSOR BASED ON INK PRINTED ELECTRODE FOR STAPHYLOCOCCAL ENTEROTOXIN DETECTIONdoi: http://dx.doi.org/10.5892/ruvrd.v12i1.1400

• Autor(a) principal: PIMENTA-MARTINS, Maria Gardenny Ribeiro; Universidade Estadual do Ceará
• Data de Publicação: 2014
• Outros Autores: FURTADO, Roselayne Ferro, DUTRA, Rosa Fireman, HENEINE, Luiz Guilherme Dias, DIAS, Ricardo Souza, BORGES, Maria de Fátima, ALVES, Carlucio Roberto
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista da Universidade Vale do Rio Verde (Online)
• Texto Completo: http://periodicos.unincor.br/index.php/revistaunincor/article/view/1400
• topic: Ciência e Tecnologia de Alimentos; Química, Física, Físico-Química e Bioquí mica dos Alim. e das Mat.-Primas Alimentares; Screen-printed carbon (SPCE); Staphylococcal enterotoxin B; Biosensor; Food safety; Analysis.; Biossensor

Staphylococcal enterotoxin is one of the more aggressive enterotoxins produced by Staphylococcus aureus strains and it is a common cause of food poisoning. Analytical methods that are sensitive, low cost and easy to use are needed to evaluate the food quality. This work describes the development of a label free immunosensor based on screen-printed AuNPs/carbon and the characterization of its analytical response for staphylococcal enterotoxin B (SEB) detection. The biosensor was constructed from self-assembled monolayer of thiols and protein A for the oriented immobilization of the polyclonal antibodies against SEB. As electrons mediator, potassium ferrocyanide was used. The electrochemical detection was direct with the parameters following: -0.2 to 0.6 V with the pulse amplitude of 0.075 V and the pulse width of 75 ms. The immunosensor showed detection and quantification limits of 0.4 µg mL-1 and 1.6 µg mL-1,respectively. The immunosensor showed quite satisfactory performance in contaminated and non-contaminated cheese samples.