Aminoguanidine: effect on granular enzymes myeloperoxidase and elastase in human neutrophils / Aminoguanidina: efeito sobre as enzimas granulares mieloperoxidase e elastase em neutrófilos humanos

• Autor(a) principal: Lima, Tayra Ferreira Oliveira
• Data de Publicação: 2021
• Outros Autores: Ferreira, Cláudia de Souza, Araújo, Tomaz Henrique, Figueiredo, Sônia Aparecida, Paula, Fernanda Borges de Araújo, Duarte, Stella Maris da Silveira, Mortara, Renato Arruda, Rodrigues, Maria Rita
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista Veras
• Texto Completo: https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/29926
• topic: Aminoguanidine; Degranulation; Myeloperoxidase; Elastase; Neutrophils.

The neutrophil is one of the main effector cells of innate immunity and has diverse mechanisms to execute microbial killing including phagocytosis, generation of reactive oxygen species (ROS) and degranulation (exocytosis of granular enzymes). Neutrophil contains granules with potentially cytotoxic substances, such as myeloperoxidase (MPO) and elastase (NE), which are released into the phagosome or into the extracellular compartments. Compounds that improve effector functions of neutrophils are an alternative for the control of infections, especially in situations of neutropenia and neutrophil dysfunction. Aminoguanidine (AMG) is a potent inhibitor of formation of advanced glycation end products (AGEs), it also has an antioxidant effect and increases the microbicidal activity of neutrophils. The aim of this study was to evaluate the effect of AMG on granular enzymes MPO and NE in human neutrophils isolated from peripheral blood. Our results showed that AMG increased the oxidative activity of MPO. In addition, AMG did not influence NE activity and had no effect on MPO and NE content. These results together suggest that AMG does not influence the neutrophil degranulation process. However, increases the MPO oxidative activity, which can play an important role in the killing of microorganisms.