Induction of diabetes in Wistar rats: is the streptozotocin model feasible at any age? / Indução de diabetes em ratos Wistar: o modelo da estreptozotocina é válido em qualquer idade?
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Veras |
Texto Completo: | https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/12114 |
Resumo: | Diabetes mellitus (DM) is an epidemic that affects millions of people worldwide. Because of its high morbidity and mortality, animal models of diabetes are indispensable to achieve a better understanding of the pathophysiological mechanisms and discover new treatments. The classic used model to induce type 1 DM involves streptozotocin (STZ) administration and it is well known that the association of streptozotocin with a high fat diet also induces type 2 DM. However, these studies are performed in rats between 60-90 days and should be performed in older animals because aging is a major risk factor for other diseases, that occur concomitantly with DM. Thus, this study evaluated diabetes in aged Wistar rats at 300 days. Diabetes was induced by an intraperitoneal injection of 60 mg/kg STZ. Three days after DM induction, the rats exhibited alterations in clinical signs and increases in the levels of aspartate and alanine aminotransferase, urea, and creatinine. Microscopic changes were observed in the kidneys and liver. These findings indicate that this model is not feasible in rats at 300 days of age and should not be attempted by other research groups or should be performed by researchers who want to study the complications of diabetes. |
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Induction of diabetes in Wistar rats: is the streptozotocin model feasible at any age? / Indução de diabetes em ratos Wistar: o modelo da estreptozotocina é válido em qualquer idade?Animal modelDrug-induced toxicityHyperinsulinemiaPreclinical studiesToxicity.Diabetes mellitus (DM) is an epidemic that affects millions of people worldwide. Because of its high morbidity and mortality, animal models of diabetes are indispensable to achieve a better understanding of the pathophysiological mechanisms and discover new treatments. The classic used model to induce type 1 DM involves streptozotocin (STZ) administration and it is well known that the association of streptozotocin with a high fat diet also induces type 2 DM. However, these studies are performed in rats between 60-90 days and should be performed in older animals because aging is a major risk factor for other diseases, that occur concomitantly with DM. Thus, this study evaluated diabetes in aged Wistar rats at 300 days. Diabetes was induced by an intraperitoneal injection of 60 mg/kg STZ. Three days after DM induction, the rats exhibited alterations in clinical signs and increases in the levels of aspartate and alanine aminotransferase, urea, and creatinine. Microscopic changes were observed in the kidneys and liver. These findings indicate that this model is not feasible in rats at 300 days of age and should not be attempted by other research groups or should be performed by researchers who want to study the complications of diabetes. Brazilian Journals Publicações de Periódicos e Editora Ltda.2020-06-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/1211410.34117/bjdv6n6-523Brazilian Journal of Development; Vol. 6 No. 6 (2020); 40153-40164Brazilian Journal of Development; Vol. 6 Núm. 6 (2020); 40153-40164Brazilian Journal of Development; v. 6 n. 6 (2020); 40153-401642525-876110.34117/bjd.v6i6reponame:Revista Verasinstname:Instituto Superior de Educação Vera Cruz (VeraCruz)instacron:VERACRUZenghttps://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/12114/10197Copyright (c) 2020 Brazilian Journal of Developmentinfo:eu-repo/semantics/openAccessMendes, Tatiane CamachoSilva, Gustavo Ratti daShirabayashi, Jaqueline de BortoliGerônimo, EdsonGermano, Ricardo de MeloLovato, Evellyn Claudia WietzikoskiAcco, AlexandraLívero, Francislaine Aparecida dos Reis2020-07-21T19:01:17Zoai:ojs2.ojs.brazilianjournals.com.br:article/12114Revistahttp://site.veracruz.edu.br:8087/instituto/revistaveras/index.php/revistaveras/PRIhttp://site.veracruz.edu.br:8087/instituto/revistaveras/index.php/revistaveras/oai||revistaveras@veracruz.edu.br2236-57292236-5729opendoar:2024-10-15T16:07:29.506169Revista Veras - Instituto Superior de Educação Vera Cruz (VeraCruz)false |
dc.title.none.fl_str_mv |
Induction of diabetes in Wistar rats: is the streptozotocin model feasible at any age? / Indução de diabetes em ratos Wistar: o modelo da estreptozotocina é válido em qualquer idade? |
title |
Induction of diabetes in Wistar rats: is the streptozotocin model feasible at any age? / Indução de diabetes em ratos Wistar: o modelo da estreptozotocina é válido em qualquer idade? |
spellingShingle |
Induction of diabetes in Wistar rats: is the streptozotocin model feasible at any age? / Indução de diabetes em ratos Wistar: o modelo da estreptozotocina é válido em qualquer idade? Mendes, Tatiane Camacho Animal model Drug-induced toxicity Hyperinsulinemia Preclinical studies Toxicity. |
title_short |
Induction of diabetes in Wistar rats: is the streptozotocin model feasible at any age? / Indução de diabetes em ratos Wistar: o modelo da estreptozotocina é válido em qualquer idade? |
title_full |
Induction of diabetes in Wistar rats: is the streptozotocin model feasible at any age? / Indução de diabetes em ratos Wistar: o modelo da estreptozotocina é válido em qualquer idade? |
title_fullStr |
Induction of diabetes in Wistar rats: is the streptozotocin model feasible at any age? / Indução de diabetes em ratos Wistar: o modelo da estreptozotocina é válido em qualquer idade? |
title_full_unstemmed |
Induction of diabetes in Wistar rats: is the streptozotocin model feasible at any age? / Indução de diabetes em ratos Wistar: o modelo da estreptozotocina é válido em qualquer idade? |
title_sort |
Induction of diabetes in Wistar rats: is the streptozotocin model feasible at any age? / Indução de diabetes em ratos Wistar: o modelo da estreptozotocina é válido em qualquer idade? |
author |
Mendes, Tatiane Camacho |
author_facet |
Mendes, Tatiane Camacho Silva, Gustavo Ratti da Shirabayashi, Jaqueline de Bortoli Gerônimo, Edson Germano, Ricardo de Melo Lovato, Evellyn Claudia Wietzikoski Acco, Alexandra Lívero, Francislaine Aparecida dos Reis |
author_role |
author |
author2 |
Silva, Gustavo Ratti da Shirabayashi, Jaqueline de Bortoli Gerônimo, Edson Germano, Ricardo de Melo Lovato, Evellyn Claudia Wietzikoski Acco, Alexandra Lívero, Francislaine Aparecida dos Reis |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Mendes, Tatiane Camacho Silva, Gustavo Ratti da Shirabayashi, Jaqueline de Bortoli Gerônimo, Edson Germano, Ricardo de Melo Lovato, Evellyn Claudia Wietzikoski Acco, Alexandra Lívero, Francislaine Aparecida dos Reis |
dc.subject.por.fl_str_mv |
Animal model Drug-induced toxicity Hyperinsulinemia Preclinical studies Toxicity. |
topic |
Animal model Drug-induced toxicity Hyperinsulinemia Preclinical studies Toxicity. |
description |
Diabetes mellitus (DM) is an epidemic that affects millions of people worldwide. Because of its high morbidity and mortality, animal models of diabetes are indispensable to achieve a better understanding of the pathophysiological mechanisms and discover new treatments. The classic used model to induce type 1 DM involves streptozotocin (STZ) administration and it is well known that the association of streptozotocin with a high fat diet also induces type 2 DM. However, these studies are performed in rats between 60-90 days and should be performed in older animals because aging is a major risk factor for other diseases, that occur concomitantly with DM. Thus, this study evaluated diabetes in aged Wistar rats at 300 days. Diabetes was induced by an intraperitoneal injection of 60 mg/kg STZ. Three days after DM induction, the rats exhibited alterations in clinical signs and increases in the levels of aspartate and alanine aminotransferase, urea, and creatinine. Microscopic changes were observed in the kidneys and liver. These findings indicate that this model is not feasible in rats at 300 days of age and should not be attempted by other research groups or should be performed by researchers who want to study the complications of diabetes. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-06-23 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/12114 10.34117/bjdv6n6-523 |
url |
https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/12114 |
identifier_str_mv |
10.34117/bjdv6n6-523 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/12114/10197 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Brazilian Journal of Development info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Brazilian Journal of Development |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Brazilian Journals Publicações de Periódicos e Editora Ltda. |
publisher.none.fl_str_mv |
Brazilian Journals Publicações de Periódicos e Editora Ltda. |
dc.source.none.fl_str_mv |
Brazilian Journal of Development; Vol. 6 No. 6 (2020); 40153-40164 Brazilian Journal of Development; Vol. 6 Núm. 6 (2020); 40153-40164 Brazilian Journal of Development; v. 6 n. 6 (2020); 40153-40164 2525-8761 10.34117/bjd.v6i6 reponame:Revista Veras instname:Instituto Superior de Educação Vera Cruz (VeraCruz) instacron:VERACRUZ |
instname_str |
Instituto Superior de Educação Vera Cruz (VeraCruz) |
instacron_str |
VERACRUZ |
institution |
VERACRUZ |
reponame_str |
Revista Veras |
collection |
Revista Veras |
repository.name.fl_str_mv |
Revista Veras - Instituto Superior de Educação Vera Cruz (VeraCruz) |
repository.mail.fl_str_mv |
||revistaveras@veracruz.edu.br |
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1813645459879624704 |