Induction of diabetes in Wistar rats: is the streptozotocin model feasible at any age? / Indução de diabetes em ratos Wistar: o modelo da estreptozotocina é válido em qualquer idade?

Detalhes bibliográficos
Autor(a) principal: Mendes, Tatiane Camacho
Data de Publicação: 2020
Outros Autores: Silva, Gustavo Ratti da, Shirabayashi, Jaqueline de Bortoli, Gerônimo, Edson, Germano, Ricardo de Melo, Lovato, Evellyn Claudia Wietzikoski, Acco, Alexandra, Lívero, Francislaine Aparecida dos Reis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Veras
Texto Completo: https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/12114
Resumo: Diabetes mellitus (DM) is an epidemic that affects millions of people worldwide. Because of its high morbidity and mortality, animal models of diabetes are indispensable to achieve a better understanding of the pathophysiological mechanisms and discover new treatments. The classic used model to induce type 1 DM involves streptozotocin (STZ) administration and it is well known that the association of streptozotocin with a high fat diet also induces type 2 DM. However, these studies are performed in rats between 60-90 days and should be performed in older animals because aging is a major risk factor for other diseases, that occur concomitantly with DM. Thus, this study evaluated diabetes in aged Wistar rats at 300 days. Diabetes was induced by an intraperitoneal injection of 60 mg/kg STZ. Three days after DM induction, the rats exhibited alterations in clinical signs and increases in the levels of aspartate and alanine aminotransferase, urea, and creatinine. Microscopic changes were observed in the kidneys and liver. These findings indicate that this model is not feasible in rats at 300 days of age and should not be attempted by other research groups or should be performed by researchers who want to study the complications of diabetes.  
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spelling Induction of diabetes in Wistar rats: is the streptozotocin model feasible at any age? / Indução de diabetes em ratos Wistar: o modelo da estreptozotocina é válido em qualquer idade?Animal modelDrug-induced toxicityHyperinsulinemiaPreclinical studiesToxicity.Diabetes mellitus (DM) is an epidemic that affects millions of people worldwide. Because of its high morbidity and mortality, animal models of diabetes are indispensable to achieve a better understanding of the pathophysiological mechanisms and discover new treatments. The classic used model to induce type 1 DM involves streptozotocin (STZ) administration and it is well known that the association of streptozotocin with a high fat diet also induces type 2 DM. However, these studies are performed in rats between 60-90 days and should be performed in older animals because aging is a major risk factor for other diseases, that occur concomitantly with DM. Thus, this study evaluated diabetes in aged Wistar rats at 300 days. Diabetes was induced by an intraperitoneal injection of 60 mg/kg STZ. Three days after DM induction, the rats exhibited alterations in clinical signs and increases in the levels of aspartate and alanine aminotransferase, urea, and creatinine. Microscopic changes were observed in the kidneys and liver. These findings indicate that this model is not feasible in rats at 300 days of age and should not be attempted by other research groups or should be performed by researchers who want to study the complications of diabetes.  Brazilian Journals Publicações de Periódicos e Editora Ltda.2020-06-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/1211410.34117/bjdv6n6-523Brazilian Journal of Development; Vol. 6 No. 6 (2020); 40153-40164Brazilian Journal of Development; Vol. 6 Núm. 6 (2020); 40153-40164Brazilian Journal of Development; v. 6 n. 6 (2020); 40153-401642525-876110.34117/bjd.v6i6reponame:Revista Verasinstname:Instituto Superior de Educação Vera Cruz (VeraCruz)instacron:VERACRUZenghttps://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/12114/10197Copyright (c) 2020 Brazilian Journal of Developmentinfo:eu-repo/semantics/openAccessMendes, Tatiane CamachoSilva, Gustavo Ratti daShirabayashi, Jaqueline de BortoliGerônimo, EdsonGermano, Ricardo de MeloLovato, Evellyn Claudia WietzikoskiAcco, AlexandraLívero, Francislaine Aparecida dos Reis2020-07-21T19:01:17Zoai:ojs2.ojs.brazilianjournals.com.br:article/12114Revistahttp://site.veracruz.edu.br:8087/instituto/revistaveras/index.php/revistaveras/PRIhttp://site.veracruz.edu.br:8087/instituto/revistaveras/index.php/revistaveras/oai||revistaveras@veracruz.edu.br2236-57292236-5729opendoar:2024-10-15T16:07:29.506169Revista Veras - Instituto Superior de Educação Vera Cruz (VeraCruz)false
dc.title.none.fl_str_mv Induction of diabetes in Wistar rats: is the streptozotocin model feasible at any age? / Indução de diabetes em ratos Wistar: o modelo da estreptozotocina é válido em qualquer idade?
title Induction of diabetes in Wistar rats: is the streptozotocin model feasible at any age? / Indução de diabetes em ratos Wistar: o modelo da estreptozotocina é válido em qualquer idade?
spellingShingle Induction of diabetes in Wistar rats: is the streptozotocin model feasible at any age? / Indução de diabetes em ratos Wistar: o modelo da estreptozotocina é válido em qualquer idade?
Mendes, Tatiane Camacho
Animal model
Drug-induced toxicity
Hyperinsulinemia
Preclinical studies
Toxicity.
title_short Induction of diabetes in Wistar rats: is the streptozotocin model feasible at any age? / Indução de diabetes em ratos Wistar: o modelo da estreptozotocina é válido em qualquer idade?
title_full Induction of diabetes in Wistar rats: is the streptozotocin model feasible at any age? / Indução de diabetes em ratos Wistar: o modelo da estreptozotocina é válido em qualquer idade?
title_fullStr Induction of diabetes in Wistar rats: is the streptozotocin model feasible at any age? / Indução de diabetes em ratos Wistar: o modelo da estreptozotocina é válido em qualquer idade?
title_full_unstemmed Induction of diabetes in Wistar rats: is the streptozotocin model feasible at any age? / Indução de diabetes em ratos Wistar: o modelo da estreptozotocina é válido em qualquer idade?
title_sort Induction of diabetes in Wistar rats: is the streptozotocin model feasible at any age? / Indução de diabetes em ratos Wistar: o modelo da estreptozotocina é válido em qualquer idade?
author Mendes, Tatiane Camacho
author_facet Mendes, Tatiane Camacho
Silva, Gustavo Ratti da
Shirabayashi, Jaqueline de Bortoli
Gerônimo, Edson
Germano, Ricardo de Melo
Lovato, Evellyn Claudia Wietzikoski
Acco, Alexandra
Lívero, Francislaine Aparecida dos Reis
author_role author
author2 Silva, Gustavo Ratti da
Shirabayashi, Jaqueline de Bortoli
Gerônimo, Edson
Germano, Ricardo de Melo
Lovato, Evellyn Claudia Wietzikoski
Acco, Alexandra
Lívero, Francislaine Aparecida dos Reis
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mendes, Tatiane Camacho
Silva, Gustavo Ratti da
Shirabayashi, Jaqueline de Bortoli
Gerônimo, Edson
Germano, Ricardo de Melo
Lovato, Evellyn Claudia Wietzikoski
Acco, Alexandra
Lívero, Francislaine Aparecida dos Reis
dc.subject.por.fl_str_mv Animal model
Drug-induced toxicity
Hyperinsulinemia
Preclinical studies
Toxicity.
topic Animal model
Drug-induced toxicity
Hyperinsulinemia
Preclinical studies
Toxicity.
description Diabetes mellitus (DM) is an epidemic that affects millions of people worldwide. Because of its high morbidity and mortality, animal models of diabetes are indispensable to achieve a better understanding of the pathophysiological mechanisms and discover new treatments. The classic used model to induce type 1 DM involves streptozotocin (STZ) administration and it is well known that the association of streptozotocin with a high fat diet also induces type 2 DM. However, these studies are performed in rats between 60-90 days and should be performed in older animals because aging is a major risk factor for other diseases, that occur concomitantly with DM. Thus, this study evaluated diabetes in aged Wistar rats at 300 days. Diabetes was induced by an intraperitoneal injection of 60 mg/kg STZ. Three days after DM induction, the rats exhibited alterations in clinical signs and increases in the levels of aspartate and alanine aminotransferase, urea, and creatinine. Microscopic changes were observed in the kidneys and liver. These findings indicate that this model is not feasible in rats at 300 days of age and should not be attempted by other research groups or should be performed by researchers who want to study the complications of diabetes.  
publishDate 2020
dc.date.none.fl_str_mv 2020-06-23
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/12114
10.34117/bjdv6n6-523
url https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/12114
identifier_str_mv 10.34117/bjdv6n6-523
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/12114/10197
dc.rights.driver.fl_str_mv Copyright (c) 2020 Brazilian Journal of Development
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Brazilian Journal of Development
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Brazilian Journals Publicações de Periódicos e Editora Ltda.
publisher.none.fl_str_mv Brazilian Journals Publicações de Periódicos e Editora Ltda.
dc.source.none.fl_str_mv Brazilian Journal of Development; Vol. 6 No. 6 (2020); 40153-40164
Brazilian Journal of Development; Vol. 6 Núm. 6 (2020); 40153-40164
Brazilian Journal of Development; v. 6 n. 6 (2020); 40153-40164
2525-8761
10.34117/bjd.v6i6
reponame:Revista Veras
instname:Instituto Superior de Educação Vera Cruz (VeraCruz)
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instname_str Instituto Superior de Educação Vera Cruz (VeraCruz)
instacron_str VERACRUZ
institution VERACRUZ
reponame_str Revista Veras
collection Revista Veras
repository.name.fl_str_mv Revista Veras - Instituto Superior de Educação Vera Cruz (VeraCruz)
repository.mail.fl_str_mv ||revistaveras@veracruz.edu.br
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