Detection of enterotoxigenic genes and mecA gene in Staphylococcus aureus isolated from goat milk / Detecção de genes enterotoxigêncos e do gene mecA em Staphylococcus aureus isolados de leite caprino
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Veras |
Texto Completo: | https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/13049 |
Resumo: | Staphylococcus aureus is a concern to food producers, because it is responsible for causing a variety of illnesses in people, among which are the toxin-mediated diseases, and because there is an increasing resistance of S. aureus to antibiotics. Thus, we aimed to detect the presence of enterotoxigenic genes and the mecA gene in S. aureus isolated from goat milk. We collected 49 samples of goat milk in which we performed a S. aureus search and resistance profile analysis by the diffusion in disks technique in Mueller-Hinton Agar using disks impregnated with the following antibiotics: sulfamethoxazole-trimethoprim (25?g), vancomycin (30?g), tetracycline (30?g), gentamicin (10?g), oxacillin (1?g), ciprofloxacin (5?g), erythromycin (15?g) and penicillin G (10?g). Next, we searched the genes of interest to the study using the Polymerase Chain Reaction (PCR). Results from the phenotypic evaluation of the resistance profile of S. aureus showed increased resistance to the antibiotics penicillin G (87.75%) and oxacillin (75.51%). S. aureus was molecularly identified by the 16S rRNA gene, with amplification in 100% of the strains isolated. We also verified the percentage of amplification for the sea, sec, sed, seg, seh, sei, sej and mecA genes. The highest percentage of amplification was of the sej gene (79.5%), followed by sei gene (48.9%). The mecA gene was detected in 4.08% of the samples. These results suggest that it is of fundamental importance to analyze the staphylococcal enterotoxins on goat milk, to protect consumers from food poisoning and to ensure public health. |
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Detection of enterotoxigenic genes and mecA gene in Staphylococcus aureus isolated from goat milk / Detecção de genes enterotoxigêncos e do gene mecA em Staphylococcus aureus isolados de leite caprinoFood poisoningMolecular biologyResistancePCR.Staphylococcus aureus is a concern to food producers, because it is responsible for causing a variety of illnesses in people, among which are the toxin-mediated diseases, and because there is an increasing resistance of S. aureus to antibiotics. Thus, we aimed to detect the presence of enterotoxigenic genes and the mecA gene in S. aureus isolated from goat milk. We collected 49 samples of goat milk in which we performed a S. aureus search and resistance profile analysis by the diffusion in disks technique in Mueller-Hinton Agar using disks impregnated with the following antibiotics: sulfamethoxazole-trimethoprim (25?g), vancomycin (30?g), tetracycline (30?g), gentamicin (10?g), oxacillin (1?g), ciprofloxacin (5?g), erythromycin (15?g) and penicillin G (10?g). Next, we searched the genes of interest to the study using the Polymerase Chain Reaction (PCR). Results from the phenotypic evaluation of the resistance profile of S. aureus showed increased resistance to the antibiotics penicillin G (87.75%) and oxacillin (75.51%). S. aureus was molecularly identified by the 16S rRNA gene, with amplification in 100% of the strains isolated. We also verified the percentage of amplification for the sea, sec, sed, seg, seh, sei, sej and mecA genes. The highest percentage of amplification was of the sej gene (79.5%), followed by sei gene (48.9%). The mecA gene was detected in 4.08% of the samples. These results suggest that it is of fundamental importance to analyze the staphylococcal enterotoxins on goat milk, to protect consumers from food poisoning and to ensure public health. Brazilian Journals Publicações de Periódicos e Editora Ltda.2020-07-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/1304910.34117/bjdv6n7-263Brazilian Journal of Development; Vol. 6 No. 7 (2020); 45692-45703Brazilian Journal of Development; Vol. 6 Núm. 7 (2020); 45692-45703Brazilian Journal of Development; v. 6 n. 7 (2020); 45692-457032525-8761reponame:Revista Verasinstname:Instituto Superior de Educação Vera Cruz (VeraCruz)instacron:VERACRUZenghttps://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/13049/10959Copyright (c) 2020 Brazilian Journal of Developmentinfo:eu-repo/semantics/openAccessRebouças, Germana GuimarãesAbrantes, Maria RocieneRebouças, Manoela de OliveiraAraújo, Bruno Vinicios Silva deSakamoto, Sidnei MiyoshiSilva, Jean Berg Alves da2020-08-11T15:17:21Zoai:ojs2.ojs.brazilianjournals.com.br:article/13049Revistahttp://site.veracruz.edu.br:8087/instituto/revistaveras/index.php/revistaveras/PRIhttp://site.veracruz.edu.br:8087/instituto/revistaveras/index.php/revistaveras/oai||revistaveras@veracruz.edu.br2236-57292236-5729opendoar:2024-10-15T16:07:55.618768Revista Veras - Instituto Superior de Educação Vera Cruz (VeraCruz)false |
dc.title.none.fl_str_mv |
Detection of enterotoxigenic genes and mecA gene in Staphylococcus aureus isolated from goat milk / Detecção de genes enterotoxigêncos e do gene mecA em Staphylococcus aureus isolados de leite caprino |
title |
Detection of enterotoxigenic genes and mecA gene in Staphylococcus aureus isolated from goat milk / Detecção de genes enterotoxigêncos e do gene mecA em Staphylococcus aureus isolados de leite caprino |
spellingShingle |
Detection of enterotoxigenic genes and mecA gene in Staphylococcus aureus isolated from goat milk / Detecção de genes enterotoxigêncos e do gene mecA em Staphylococcus aureus isolados de leite caprino Rebouças, Germana Guimarães Food poisoning Molecular biology Resistance PCR. |
title_short |
Detection of enterotoxigenic genes and mecA gene in Staphylococcus aureus isolated from goat milk / Detecção de genes enterotoxigêncos e do gene mecA em Staphylococcus aureus isolados de leite caprino |
title_full |
Detection of enterotoxigenic genes and mecA gene in Staphylococcus aureus isolated from goat milk / Detecção de genes enterotoxigêncos e do gene mecA em Staphylococcus aureus isolados de leite caprino |
title_fullStr |
Detection of enterotoxigenic genes and mecA gene in Staphylococcus aureus isolated from goat milk / Detecção de genes enterotoxigêncos e do gene mecA em Staphylococcus aureus isolados de leite caprino |
title_full_unstemmed |
Detection of enterotoxigenic genes and mecA gene in Staphylococcus aureus isolated from goat milk / Detecção de genes enterotoxigêncos e do gene mecA em Staphylococcus aureus isolados de leite caprino |
title_sort |
Detection of enterotoxigenic genes and mecA gene in Staphylococcus aureus isolated from goat milk / Detecção de genes enterotoxigêncos e do gene mecA em Staphylococcus aureus isolados de leite caprino |
author |
Rebouças, Germana Guimarães |
author_facet |
Rebouças, Germana Guimarães Abrantes, Maria Rociene Rebouças, Manoela de Oliveira Araújo, Bruno Vinicios Silva de Sakamoto, Sidnei Miyoshi Silva, Jean Berg Alves da |
author_role |
author |
author2 |
Abrantes, Maria Rociene Rebouças, Manoela de Oliveira Araújo, Bruno Vinicios Silva de Sakamoto, Sidnei Miyoshi Silva, Jean Berg Alves da |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Rebouças, Germana Guimarães Abrantes, Maria Rociene Rebouças, Manoela de Oliveira Araújo, Bruno Vinicios Silva de Sakamoto, Sidnei Miyoshi Silva, Jean Berg Alves da |
dc.subject.por.fl_str_mv |
Food poisoning Molecular biology Resistance PCR. |
topic |
Food poisoning Molecular biology Resistance PCR. |
description |
Staphylococcus aureus is a concern to food producers, because it is responsible for causing a variety of illnesses in people, among which are the toxin-mediated diseases, and because there is an increasing resistance of S. aureus to antibiotics. Thus, we aimed to detect the presence of enterotoxigenic genes and the mecA gene in S. aureus isolated from goat milk. We collected 49 samples of goat milk in which we performed a S. aureus search and resistance profile analysis by the diffusion in disks technique in Mueller-Hinton Agar using disks impregnated with the following antibiotics: sulfamethoxazole-trimethoprim (25?g), vancomycin (30?g), tetracycline (30?g), gentamicin (10?g), oxacillin (1?g), ciprofloxacin (5?g), erythromycin (15?g) and penicillin G (10?g). Next, we searched the genes of interest to the study using the Polymerase Chain Reaction (PCR). Results from the phenotypic evaluation of the resistance profile of S. aureus showed increased resistance to the antibiotics penicillin G (87.75%) and oxacillin (75.51%). S. aureus was molecularly identified by the 16S rRNA gene, with amplification in 100% of the strains isolated. We also verified the percentage of amplification for the sea, sec, sed, seg, seh, sei, sej and mecA genes. The highest percentage of amplification was of the sej gene (79.5%), followed by sei gene (48.9%). The mecA gene was detected in 4.08% of the samples. These results suggest that it is of fundamental importance to analyze the staphylococcal enterotoxins on goat milk, to protect consumers from food poisoning and to ensure public health. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-07-13 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/13049 10.34117/bjdv6n7-263 |
url |
https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/13049 |
identifier_str_mv |
10.34117/bjdv6n7-263 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/13049/10959 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Brazilian Journal of Development info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Brazilian Journal of Development |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Brazilian Journals Publicações de Periódicos e Editora Ltda. |
publisher.none.fl_str_mv |
Brazilian Journals Publicações de Periódicos e Editora Ltda. |
dc.source.none.fl_str_mv |
Brazilian Journal of Development; Vol. 6 No. 7 (2020); 45692-45703 Brazilian Journal of Development; Vol. 6 Núm. 7 (2020); 45692-45703 Brazilian Journal of Development; v. 6 n. 7 (2020); 45692-45703 2525-8761 reponame:Revista Veras instname:Instituto Superior de Educação Vera Cruz (VeraCruz) instacron:VERACRUZ |
instname_str |
Instituto Superior de Educação Vera Cruz (VeraCruz) |
instacron_str |
VERACRUZ |
institution |
VERACRUZ |
reponame_str |
Revista Veras |
collection |
Revista Veras |
repository.name.fl_str_mv |
Revista Veras - Instituto Superior de Educação Vera Cruz (VeraCruz) |
repository.mail.fl_str_mv |
||revistaveras@veracruz.edu.br |
_version_ |
1813645463285399552 |