Detection of enterotoxigenic genes and mecA gene in Staphylococcus aureus isolated from goat milk / Detecção de genes enterotoxigêncos e do gene mecA em Staphylococcus aureus isolados de leite caprino

Detalhes bibliográficos
Autor(a) principal: Rebouças, Germana Guimarães
Data de Publicação: 2020
Outros Autores: Abrantes, Maria Rociene, Rebouças, Manoela de Oliveira, Araújo, Bruno Vinicios Silva de, Sakamoto, Sidnei Miyoshi, Silva, Jean Berg Alves da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Veras
Texto Completo: https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/13049
Resumo: Staphylococcus aureus is a concern to food producers, because it is responsible for causing a variety of illnesses in people, among which are the toxin-mediated diseases, and because there is an increasing resistance of S. aureus to antibiotics. Thus, we aimed to detect the presence of enterotoxigenic genes and the mecA gene in S. aureus isolated from goat milk. We collected 49 samples of goat milk in which we performed a S. aureus search and resistance profile analysis by the diffusion in disks technique in Mueller-Hinton Agar using disks impregnated with the following antibiotics: sulfamethoxazole-trimethoprim (25?g), vancomycin (30?g), tetracycline (30?g), gentamicin (10?g), oxacillin (1?g), ciprofloxacin (5?g), erythromycin (15?g) and penicillin G (10?g). Next, we searched the genes of interest to the study using the Polymerase Chain Reaction (PCR). Results from the phenotypic evaluation of the resistance profile of S. aureus showed increased resistance to the antibiotics penicillin G (87.75%) and oxacillin (75.51%). S. aureus was molecularly identified by the 16S rRNA gene, with amplification in 100% of the strains isolated. We also verified the percentage of amplification for the sea, sec, sed, seg, seh, sei, sej and mecA genes. The highest percentage of amplification was of the sej gene (79.5%), followed by sei gene (48.9%). The mecA gene was detected in 4.08% of the samples. These results suggest that it is of fundamental importance to analyze the staphylococcal enterotoxins on goat milk, to protect consumers from food poisoning and to ensure public health.  
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spelling Detection of enterotoxigenic genes and mecA gene in Staphylococcus aureus isolated from goat milk / Detecção de genes enterotoxigêncos e do gene mecA em Staphylococcus aureus isolados de leite caprinoFood poisoningMolecular biologyResistancePCR.Staphylococcus aureus is a concern to food producers, because it is responsible for causing a variety of illnesses in people, among which are the toxin-mediated diseases, and because there is an increasing resistance of S. aureus to antibiotics. Thus, we aimed to detect the presence of enterotoxigenic genes and the mecA gene in S. aureus isolated from goat milk. We collected 49 samples of goat milk in which we performed a S. aureus search and resistance profile analysis by the diffusion in disks technique in Mueller-Hinton Agar using disks impregnated with the following antibiotics: sulfamethoxazole-trimethoprim (25?g), vancomycin (30?g), tetracycline (30?g), gentamicin (10?g), oxacillin (1?g), ciprofloxacin (5?g), erythromycin (15?g) and penicillin G (10?g). Next, we searched the genes of interest to the study using the Polymerase Chain Reaction (PCR). Results from the phenotypic evaluation of the resistance profile of S. aureus showed increased resistance to the antibiotics penicillin G (87.75%) and oxacillin (75.51%). S. aureus was molecularly identified by the 16S rRNA gene, with amplification in 100% of the strains isolated. We also verified the percentage of amplification for the sea, sec, sed, seg, seh, sei, sej and mecA genes. The highest percentage of amplification was of the sej gene (79.5%), followed by sei gene (48.9%). The mecA gene was detected in 4.08% of the samples. These results suggest that it is of fundamental importance to analyze the staphylococcal enterotoxins on goat milk, to protect consumers from food poisoning and to ensure public health.  Brazilian Journals Publicações de Periódicos e Editora Ltda.2020-07-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/1304910.34117/bjdv6n7-263Brazilian Journal of Development; Vol. 6 No. 7 (2020); 45692-45703Brazilian Journal of Development; Vol. 6 Núm. 7 (2020); 45692-45703Brazilian Journal of Development; v. 6 n. 7 (2020); 45692-457032525-8761reponame:Revista Verasinstname:Instituto Superior de Educação Vera Cruz (VeraCruz)instacron:VERACRUZenghttps://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/13049/10959Copyright (c) 2020 Brazilian Journal of Developmentinfo:eu-repo/semantics/openAccessRebouças, Germana GuimarãesAbrantes, Maria RocieneRebouças, Manoela de OliveiraAraújo, Bruno Vinicios Silva deSakamoto, Sidnei MiyoshiSilva, Jean Berg Alves da2020-08-11T15:17:21Zoai:ojs2.ojs.brazilianjournals.com.br:article/13049Revistahttp://site.veracruz.edu.br:8087/instituto/revistaveras/index.php/revistaveras/PRIhttp://site.veracruz.edu.br:8087/instituto/revistaveras/index.php/revistaveras/oai||revistaveras@veracruz.edu.br2236-57292236-5729opendoar:2024-10-15T16:07:55.618768Revista Veras - Instituto Superior de Educação Vera Cruz (VeraCruz)false
dc.title.none.fl_str_mv Detection of enterotoxigenic genes and mecA gene in Staphylococcus aureus isolated from goat milk / Detecção de genes enterotoxigêncos e do gene mecA em Staphylococcus aureus isolados de leite caprino
title Detection of enterotoxigenic genes and mecA gene in Staphylococcus aureus isolated from goat milk / Detecção de genes enterotoxigêncos e do gene mecA em Staphylococcus aureus isolados de leite caprino
spellingShingle Detection of enterotoxigenic genes and mecA gene in Staphylococcus aureus isolated from goat milk / Detecção de genes enterotoxigêncos e do gene mecA em Staphylococcus aureus isolados de leite caprino
Rebouças, Germana Guimarães
Food poisoning
Molecular biology
Resistance
PCR.
title_short Detection of enterotoxigenic genes and mecA gene in Staphylococcus aureus isolated from goat milk / Detecção de genes enterotoxigêncos e do gene mecA em Staphylococcus aureus isolados de leite caprino
title_full Detection of enterotoxigenic genes and mecA gene in Staphylococcus aureus isolated from goat milk / Detecção de genes enterotoxigêncos e do gene mecA em Staphylococcus aureus isolados de leite caprino
title_fullStr Detection of enterotoxigenic genes and mecA gene in Staphylococcus aureus isolated from goat milk / Detecção de genes enterotoxigêncos e do gene mecA em Staphylococcus aureus isolados de leite caprino
title_full_unstemmed Detection of enterotoxigenic genes and mecA gene in Staphylococcus aureus isolated from goat milk / Detecção de genes enterotoxigêncos e do gene mecA em Staphylococcus aureus isolados de leite caprino
title_sort Detection of enterotoxigenic genes and mecA gene in Staphylococcus aureus isolated from goat milk / Detecção de genes enterotoxigêncos e do gene mecA em Staphylococcus aureus isolados de leite caprino
author Rebouças, Germana Guimarães
author_facet Rebouças, Germana Guimarães
Abrantes, Maria Rociene
Rebouças, Manoela de Oliveira
Araújo, Bruno Vinicios Silva de
Sakamoto, Sidnei Miyoshi
Silva, Jean Berg Alves da
author_role author
author2 Abrantes, Maria Rociene
Rebouças, Manoela de Oliveira
Araújo, Bruno Vinicios Silva de
Sakamoto, Sidnei Miyoshi
Silva, Jean Berg Alves da
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Rebouças, Germana Guimarães
Abrantes, Maria Rociene
Rebouças, Manoela de Oliveira
Araújo, Bruno Vinicios Silva de
Sakamoto, Sidnei Miyoshi
Silva, Jean Berg Alves da
dc.subject.por.fl_str_mv Food poisoning
Molecular biology
Resistance
PCR.
topic Food poisoning
Molecular biology
Resistance
PCR.
description Staphylococcus aureus is a concern to food producers, because it is responsible for causing a variety of illnesses in people, among which are the toxin-mediated diseases, and because there is an increasing resistance of S. aureus to antibiotics. Thus, we aimed to detect the presence of enterotoxigenic genes and the mecA gene in S. aureus isolated from goat milk. We collected 49 samples of goat milk in which we performed a S. aureus search and resistance profile analysis by the diffusion in disks technique in Mueller-Hinton Agar using disks impregnated with the following antibiotics: sulfamethoxazole-trimethoprim (25?g), vancomycin (30?g), tetracycline (30?g), gentamicin (10?g), oxacillin (1?g), ciprofloxacin (5?g), erythromycin (15?g) and penicillin G (10?g). Next, we searched the genes of interest to the study using the Polymerase Chain Reaction (PCR). Results from the phenotypic evaluation of the resistance profile of S. aureus showed increased resistance to the antibiotics penicillin G (87.75%) and oxacillin (75.51%). S. aureus was molecularly identified by the 16S rRNA gene, with amplification in 100% of the strains isolated. We also verified the percentage of amplification for the sea, sec, sed, seg, seh, sei, sej and mecA genes. The highest percentage of amplification was of the sej gene (79.5%), followed by sei gene (48.9%). The mecA gene was detected in 4.08% of the samples. These results suggest that it is of fundamental importance to analyze the staphylococcal enterotoxins on goat milk, to protect consumers from food poisoning and to ensure public health.  
publishDate 2020
dc.date.none.fl_str_mv 2020-07-13
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/13049
10.34117/bjdv6n7-263
url https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/13049
identifier_str_mv 10.34117/bjdv6n7-263
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/13049/10959
dc.rights.driver.fl_str_mv Copyright (c) 2020 Brazilian Journal of Development
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Brazilian Journal of Development
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Brazilian Journals Publicações de Periódicos e Editora Ltda.
publisher.none.fl_str_mv Brazilian Journals Publicações de Periódicos e Editora Ltda.
dc.source.none.fl_str_mv Brazilian Journal of Development; Vol. 6 No. 7 (2020); 45692-45703
Brazilian Journal of Development; Vol. 6 Núm. 7 (2020); 45692-45703
Brazilian Journal of Development; v. 6 n. 7 (2020); 45692-45703
2525-8761
reponame:Revista Veras
instname:Instituto Superior de Educação Vera Cruz (VeraCruz)
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instname_str Instituto Superior de Educação Vera Cruz (VeraCruz)
instacron_str VERACRUZ
institution VERACRUZ
reponame_str Revista Veras
collection Revista Veras
repository.name.fl_str_mv Revista Veras - Instituto Superior de Educação Vera Cruz (VeraCruz)
repository.mail.fl_str_mv ||revistaveras@veracruz.edu.br
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