New model of biological membrane (shed snakeskin) for studies of antioxidant activity in photoprotective formulation/ Novo modelo de membrana biológica (ecdise de pele de cobra) para estudos de atividade antioxidante em formulação fotoprotetora
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Veras |
Texto Completo: | https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/29023 |
Resumo: | Antioxidants of natural origin are used in medicines and cosmetics with several benefits, such as: photoprotective action, anti-aging, moisturizing and anti-pollutant. The human epidermis has an important barrier effect and limited anti-oxidative capacity, so studies with the epidermis is essential. Shed snakeskin (SS) is composed of the stratum corneum and provide a barrier like human stratum corneum. This alternative does not show a tendency to microbiological degradation and can be considered ecologically correct. This study intends to present, in an innovative way, the Electron Paramagnetic Resonance spectroscopy (EPR) and The Forster Resonance Energy Transfer (FRET) were employed to evaluate the natural antioxidant substances (Resveratrol/ RES 3.0 w/w and Ferulic acid/ FA 1.0 w/w) associated with organic sunscreens ingredients (Ethylhexyl Methoxycinnamate/ EHMC 10.0%w/w and Butyl Methoxydibenzoylmethano/ BMBM 5.0%w/w in a photoprotective emulsion (PB). Furthermore, the use of SS seedlings as a possible alternative to the use of human or animal skin ex-vivo. RES and FA can absorb the energy emitted by the EHMC in FRET, preventing the passage through the triplet state, favoring the photostability of this sunscreen, the same not ocorred with the BMBM. Antioxidant activity of the photoprotective formulations was evaluated in vitro by the percentual inhibition of the radical 2,2-diphenyl-1-picrihydrazyl (DPPH•). The antioxidant activity with RES, 97.0% inhibition of DPPH• in the PB, was higher than PB + FA (91.0%), however the concentration of RES in PB was higher than FA. The sample SS + PB + FA was the one with the lowest number of free radicals after irradiation, which corroborated the high percentage of radical inhibition in vitro and it was the better association with the photoprotective formulation. |
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New model of biological membrane (shed snakeskin) for studies of antioxidant activity in photoprotective formulation/ Novo modelo de membrana biológica (ecdise de pele de cobra) para estudos de atividade antioxidante em formulação fotoprotetorasnakeskinEPRphotoprotective formulationFerulic acidresveratrol.Antioxidants of natural origin are used in medicines and cosmetics with several benefits, such as: photoprotective action, anti-aging, moisturizing and anti-pollutant. The human epidermis has an important barrier effect and limited anti-oxidative capacity, so studies with the epidermis is essential. Shed snakeskin (SS) is composed of the stratum corneum and provide a barrier like human stratum corneum. This alternative does not show a tendency to microbiological degradation and can be considered ecologically correct. This study intends to present, in an innovative way, the Electron Paramagnetic Resonance spectroscopy (EPR) and The Forster Resonance Energy Transfer (FRET) were employed to evaluate the natural antioxidant substances (Resveratrol/ RES 3.0 w/w and Ferulic acid/ FA 1.0 w/w) associated with organic sunscreens ingredients (Ethylhexyl Methoxycinnamate/ EHMC 10.0%w/w and Butyl Methoxydibenzoylmethano/ BMBM 5.0%w/w in a photoprotective emulsion (PB). Furthermore, the use of SS seedlings as a possible alternative to the use of human or animal skin ex-vivo. RES and FA can absorb the energy emitted by the EHMC in FRET, preventing the passage through the triplet state, favoring the photostability of this sunscreen, the same not ocorred with the BMBM. Antioxidant activity of the photoprotective formulations was evaluated in vitro by the percentual inhibition of the radical 2,2-diphenyl-1-picrihydrazyl (DPPH•). The antioxidant activity with RES, 97.0% inhibition of DPPH• in the PB, was higher than PB + FA (91.0%), however the concentration of RES in PB was higher than FA. The sample SS + PB + FA was the one with the lowest number of free radicals after irradiation, which corroborated the high percentage of radical inhibition in vitro and it was the better association with the photoprotective formulation. Brazilian Journals Publicações de Periódicos e Editora Ltda.2021-04-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/2902310.34117/bjdv7n4-674Brazilian Journal of Development; Vol. 7 No. 4 (2021); 43214-43236Brazilian Journal of Development; Vol. 7 Núm. 4 (2021); 43214-43236Brazilian Journal of Development; v. 7 n. 4 (2021); 43214-432362525-876110.34117/bjd.v7i4reponame:Revista Verasinstname:Instituto Superior de Educação Vera Cruz (VeraCruz)instacron:VERACRUZenghttps://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/29023/23087Copyright (c) 2021 Brazilian Journal of Developmentinfo:eu-repo/semantics/openAccessFreire, Thamires BatelloJúnior, Orlando RodriguesPinto, Claudinéia Aparecida Sales de OliveiraEscudeiro, Cassiano CarlosBaby, André RolimVelasco, Maria Valéria Robles2021-07-28T18:56:47Zoai:ojs2.ojs.brazilianjournals.com.br:article/29023Revistahttp://site.veracruz.edu.br:8087/instituto/revistaveras/index.php/revistaveras/PRIhttp://site.veracruz.edu.br:8087/instituto/revistaveras/index.php/revistaveras/oai||revistaveras@veracruz.edu.br2236-57292236-5729opendoar:2024-10-15T16:15:29.291790Revista Veras - Instituto Superior de Educação Vera Cruz (VeraCruz)false |
dc.title.none.fl_str_mv |
New model of biological membrane (shed snakeskin) for studies of antioxidant activity in photoprotective formulation/ Novo modelo de membrana biológica (ecdise de pele de cobra) para estudos de atividade antioxidante em formulação fotoprotetora |
title |
New model of biological membrane (shed snakeskin) for studies of antioxidant activity in photoprotective formulation/ Novo modelo de membrana biológica (ecdise de pele de cobra) para estudos de atividade antioxidante em formulação fotoprotetora |
spellingShingle |
New model of biological membrane (shed snakeskin) for studies of antioxidant activity in photoprotective formulation/ Novo modelo de membrana biológica (ecdise de pele de cobra) para estudos de atividade antioxidante em formulação fotoprotetora Freire, Thamires Batello snakeskin EPR photoprotective formulation Ferulic acid resveratrol. |
title_short |
New model of biological membrane (shed snakeskin) for studies of antioxidant activity in photoprotective formulation/ Novo modelo de membrana biológica (ecdise de pele de cobra) para estudos de atividade antioxidante em formulação fotoprotetora |
title_full |
New model of biological membrane (shed snakeskin) for studies of antioxidant activity in photoprotective formulation/ Novo modelo de membrana biológica (ecdise de pele de cobra) para estudos de atividade antioxidante em formulação fotoprotetora |
title_fullStr |
New model of biological membrane (shed snakeskin) for studies of antioxidant activity in photoprotective formulation/ Novo modelo de membrana biológica (ecdise de pele de cobra) para estudos de atividade antioxidante em formulação fotoprotetora |
title_full_unstemmed |
New model of biological membrane (shed snakeskin) for studies of antioxidant activity in photoprotective formulation/ Novo modelo de membrana biológica (ecdise de pele de cobra) para estudos de atividade antioxidante em formulação fotoprotetora |
title_sort |
New model of biological membrane (shed snakeskin) for studies of antioxidant activity in photoprotective formulation/ Novo modelo de membrana biológica (ecdise de pele de cobra) para estudos de atividade antioxidante em formulação fotoprotetora |
author |
Freire, Thamires Batello |
author_facet |
Freire, Thamires Batello Júnior, Orlando Rodrigues Pinto, Claudinéia Aparecida Sales de Oliveira Escudeiro, Cassiano Carlos Baby, André Rolim Velasco, Maria Valéria Robles |
author_role |
author |
author2 |
Júnior, Orlando Rodrigues Pinto, Claudinéia Aparecida Sales de Oliveira Escudeiro, Cassiano Carlos Baby, André Rolim Velasco, Maria Valéria Robles |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Freire, Thamires Batello Júnior, Orlando Rodrigues Pinto, Claudinéia Aparecida Sales de Oliveira Escudeiro, Cassiano Carlos Baby, André Rolim Velasco, Maria Valéria Robles |
dc.subject.por.fl_str_mv |
snakeskin EPR photoprotective formulation Ferulic acid resveratrol. |
topic |
snakeskin EPR photoprotective formulation Ferulic acid resveratrol. |
description |
Antioxidants of natural origin are used in medicines and cosmetics with several benefits, such as: photoprotective action, anti-aging, moisturizing and anti-pollutant. The human epidermis has an important barrier effect and limited anti-oxidative capacity, so studies with the epidermis is essential. Shed snakeskin (SS) is composed of the stratum corneum and provide a barrier like human stratum corneum. This alternative does not show a tendency to microbiological degradation and can be considered ecologically correct. This study intends to present, in an innovative way, the Electron Paramagnetic Resonance spectroscopy (EPR) and The Forster Resonance Energy Transfer (FRET) were employed to evaluate the natural antioxidant substances (Resveratrol/ RES 3.0 w/w and Ferulic acid/ FA 1.0 w/w) associated with organic sunscreens ingredients (Ethylhexyl Methoxycinnamate/ EHMC 10.0%w/w and Butyl Methoxydibenzoylmethano/ BMBM 5.0%w/w in a photoprotective emulsion (PB). Furthermore, the use of SS seedlings as a possible alternative to the use of human or animal skin ex-vivo. RES and FA can absorb the energy emitted by the EHMC in FRET, preventing the passage through the triplet state, favoring the photostability of this sunscreen, the same not ocorred with the BMBM. Antioxidant activity of the photoprotective formulations was evaluated in vitro by the percentual inhibition of the radical 2,2-diphenyl-1-picrihydrazyl (DPPH•). The antioxidant activity with RES, 97.0% inhibition of DPPH• in the PB, was higher than PB + FA (91.0%), however the concentration of RES in PB was higher than FA. The sample SS + PB + FA was the one with the lowest number of free radicals after irradiation, which corroborated the high percentage of radical inhibition in vitro and it was the better association with the photoprotective formulation. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-04-29 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/29023 10.34117/bjdv7n4-674 |
url |
https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/29023 |
identifier_str_mv |
10.34117/bjdv7n4-674 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/29023/23087 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Brazilian Journal of Development info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Brazilian Journal of Development |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Brazilian Journals Publicações de Periódicos e Editora Ltda. |
publisher.none.fl_str_mv |
Brazilian Journals Publicações de Periódicos e Editora Ltda. |
dc.source.none.fl_str_mv |
Brazilian Journal of Development; Vol. 7 No. 4 (2021); 43214-43236 Brazilian Journal of Development; Vol. 7 Núm. 4 (2021); 43214-43236 Brazilian Journal of Development; v. 7 n. 4 (2021); 43214-43236 2525-8761 10.34117/bjd.v7i4 reponame:Revista Veras instname:Instituto Superior de Educação Vera Cruz (VeraCruz) instacron:VERACRUZ |
instname_str |
Instituto Superior de Educação Vera Cruz (VeraCruz) |
instacron_str |
VERACRUZ |
institution |
VERACRUZ |
reponame_str |
Revista Veras |
collection |
Revista Veras |
repository.name.fl_str_mv |
Revista Veras - Instituto Superior de Educação Vera Cruz (VeraCruz) |
repository.mail.fl_str_mv |
||revistaveras@veracruz.edu.br |
_version_ |
1813645528586518528 |