Development and validation of high-performance liquid chromatography method for determination of atorvastatin in polymeric nanocapsules / Desenvolvimento e validação de método de cromatografia líquida de alta eficiência para determinação de atorvastatina em nanocápsulas poliméricas

• Autor(a) principal: Viçozzi, Gabriel Pedroso
• Data de Publicação: 2020
• Outros Autores: Neto, Luiz Torres, Silva, Pedro Santos da, Nenê, Luiza Rodrigues, Colomé, Letícia Marques, Bender, Eduardo André
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista Veras
• Texto Completo: https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/15894
• topic: HPLC method validation; atorvastatin; nanocapsules; essential oils.

This study aimed to develop and validate a HPLC based analytical methodology for determination of atorvastatin within nanocapsules containing ginger or grape seed essential oils (NCAtG and NCAtU, respectively). Chromatographic separation was achieved by employing a Venusil XBR C18 column as stationary phase and a ternary mixture of acetonitrile, 0.1% phosphoric acid, and methanol (60:30:10) as the mobile phase. The validated method proved to be linear for atorvastatin in the range of both particles were 10 to 50 ?g.mL?1. Detection and quantification limits for atorvastatin were, respectively, 30 ng.mL?1 and 300 ng.mL?1 for NCAtG, while for NCAtU the values were 39 ng.mL?1 and 390 ng.mL?1, what assures the methodology sensitivity. The method was also precise (1.28% RSD for NCAtG and NCAtU), besides showing recovery rates close to 100% for both nanocapsules formulations when accuracy was accessed. Minor alterations over chromatographic (pH, mobile phase, flow and detector) setup have confirmed methodology robustness. The present methodology was able to detect and quantify atorvastatin in nanocapsules containing ginger or grape seed essential oils. Thus, proving to be an analytical alternative for the quality control of this dosage form.