Bioprospection of cellulose-decomposers soil bacteria / Bioprospecção de bactérias do solo degradadoras de celulose
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Veras |
Texto Completo: | https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/13555 |
Resumo: | Soil microorganisms have a high bacterial biodiversity and an important group is the cellulose-decomposers bacteria, which through their endoglucanases, exoglucanases and ?-glycosidases enzymatic complexes are responsible for the planet organic biomass degradation. Therefore, to understand and apply their potentials in the biotechnology area, is necessary to know their biologic, biochemical and environmental characteristics and proprieties of the microorganisms involved in these degradation processes. Ten bacteria isolates where obtained. To verify the degradation, the filter paper and solid medium Luria-Bertani was used. In the extracellular enzymatic production analysis, the substrate used was the Whatman N°1 filter paper for the ß-1,4 exoglucanase and carboxymethyl cellulose for the ß-1,4 endoglucanase. Also the microbial biomass analysis was made. The results showed that, the most effective cellulose degradation isolates having as substrates, the BC5 and the BC2 bacteria respectively. In the endoglucanase ß-1,4 enzymatic activity was the BC5 and for the ß-1,4 exoglucanase was the BC8, meanwhile for the microbial biomass production, the best result was for the BC9 isolate bacteria. The results showed that between the different bacteria species from the different locals, significant differences were presented, when each of the analysis were compared and therefore this can be associated to the different types of ecosystems and to the biochemical and environmental characteristics in each type of soil, having different mechanisms directly related with the cellulose degradation. Therefore, these microorganisms have a potential use in diverse biotechnological and bioremediation cellulose degradation processes. |
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Bioprospection of cellulose-decomposers soil bacteria / Bioprospecção de bactérias do solo degradadoras de celulosecellulolytic enzymescellulolytic microorganismsendoglucanaseexoglucanasesoilmicrobiology environmental.Soil microorganisms have a high bacterial biodiversity and an important group is the cellulose-decomposers bacteria, which through their endoglucanases, exoglucanases and ?-glycosidases enzymatic complexes are responsible for the planet organic biomass degradation. Therefore, to understand and apply their potentials in the biotechnology area, is necessary to know their biologic, biochemical and environmental characteristics and proprieties of the microorganisms involved in these degradation processes. Ten bacteria isolates where obtained. To verify the degradation, the filter paper and solid medium Luria-Bertani was used. In the extracellular enzymatic production analysis, the substrate used was the Whatman N°1 filter paper for the ß-1,4 exoglucanase and carboxymethyl cellulose for the ß-1,4 endoglucanase. Also the microbial biomass analysis was made. The results showed that, the most effective cellulose degradation isolates having as substrates, the BC5 and the BC2 bacteria respectively. In the endoglucanase ß-1,4 enzymatic activity was the BC5 and for the ß-1,4 exoglucanase was the BC8, meanwhile for the microbial biomass production, the best result was for the BC9 isolate bacteria. The results showed that between the different bacteria species from the different locals, significant differences were presented, when each of the analysis were compared and therefore this can be associated to the different types of ecosystems and to the biochemical and environmental characteristics in each type of soil, having different mechanisms directly related with the cellulose degradation. Therefore, these microorganisms have a potential use in diverse biotechnological and bioremediation cellulose degradation processes. Brazilian Journals Publicações de Periódicos e Editora Ltda.2020-07-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/1355510.34117/bjdv6n7-506Brazilian Journal of Development; Vol. 6 No. 7 (2020); 48831-48851Brazilian Journal of Development; Vol. 6 Núm. 7 (2020); 48831-48851Brazilian Journal of Development; v. 6 n. 7 (2020); 48831-488512525-8761reponame:Revista Verasinstname:Instituto Superior de Educação Vera Cruz (VeraCruz)instacron:VERACRUZenghttps://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/13555/11358Copyright (c) 2020 Brazilian Journal of Developmentinfo:eu-repo/semantics/openAccessHernández, Yohana Melania LópezKuss, Anelise VicentiniPeil, Greice Hartwig SchwankeVillarreal, José PabloGamaro, Giovana Duzzo2020-08-11T15:17:25Zoai:ojs2.ojs.brazilianjournals.com.br:article/13555Revistahttp://site.veracruz.edu.br:8087/instituto/revistaveras/index.php/revistaveras/PRIhttp://site.veracruz.edu.br:8087/instituto/revistaveras/index.php/revistaveras/oai||revistaveras@veracruz.edu.br2236-57292236-5729opendoar:2024-10-15T16:08:10.519738Revista Veras - Instituto Superior de Educação Vera Cruz (VeraCruz)false |
dc.title.none.fl_str_mv |
Bioprospection of cellulose-decomposers soil bacteria / Bioprospecção de bactérias do solo degradadoras de celulose |
title |
Bioprospection of cellulose-decomposers soil bacteria / Bioprospecção de bactérias do solo degradadoras de celulose |
spellingShingle |
Bioprospection of cellulose-decomposers soil bacteria / Bioprospecção de bactérias do solo degradadoras de celulose Hernández, Yohana Melania López cellulolytic enzymes cellulolytic microorganisms endoglucanase exoglucanase soil microbiology environmental. |
title_short |
Bioprospection of cellulose-decomposers soil bacteria / Bioprospecção de bactérias do solo degradadoras de celulose |
title_full |
Bioprospection of cellulose-decomposers soil bacteria / Bioprospecção de bactérias do solo degradadoras de celulose |
title_fullStr |
Bioprospection of cellulose-decomposers soil bacteria / Bioprospecção de bactérias do solo degradadoras de celulose |
title_full_unstemmed |
Bioprospection of cellulose-decomposers soil bacteria / Bioprospecção de bactérias do solo degradadoras de celulose |
title_sort |
Bioprospection of cellulose-decomposers soil bacteria / Bioprospecção de bactérias do solo degradadoras de celulose |
author |
Hernández, Yohana Melania López |
author_facet |
Hernández, Yohana Melania López Kuss, Anelise Vicentini Peil, Greice Hartwig Schwanke Villarreal, José Pablo Gamaro, Giovana Duzzo |
author_role |
author |
author2 |
Kuss, Anelise Vicentini Peil, Greice Hartwig Schwanke Villarreal, José Pablo Gamaro, Giovana Duzzo |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Hernández, Yohana Melania López Kuss, Anelise Vicentini Peil, Greice Hartwig Schwanke Villarreal, José Pablo Gamaro, Giovana Duzzo |
dc.subject.por.fl_str_mv |
cellulolytic enzymes cellulolytic microorganisms endoglucanase exoglucanase soil microbiology environmental. |
topic |
cellulolytic enzymes cellulolytic microorganisms endoglucanase exoglucanase soil microbiology environmental. |
description |
Soil microorganisms have a high bacterial biodiversity and an important group is the cellulose-decomposers bacteria, which through their endoglucanases, exoglucanases and ?-glycosidases enzymatic complexes are responsible for the planet organic biomass degradation. Therefore, to understand and apply their potentials in the biotechnology area, is necessary to know their biologic, biochemical and environmental characteristics and proprieties of the microorganisms involved in these degradation processes. Ten bacteria isolates where obtained. To verify the degradation, the filter paper and solid medium Luria-Bertani was used. In the extracellular enzymatic production analysis, the substrate used was the Whatman N°1 filter paper for the ß-1,4 exoglucanase and carboxymethyl cellulose for the ß-1,4 endoglucanase. Also the microbial biomass analysis was made. The results showed that, the most effective cellulose degradation isolates having as substrates, the BC5 and the BC2 bacteria respectively. In the endoglucanase ß-1,4 enzymatic activity was the BC5 and for the ß-1,4 exoglucanase was the BC8, meanwhile for the microbial biomass production, the best result was for the BC9 isolate bacteria. The results showed that between the different bacteria species from the different locals, significant differences were presented, when each of the analysis were compared and therefore this can be associated to the different types of ecosystems and to the biochemical and environmental characteristics in each type of soil, having different mechanisms directly related with the cellulose degradation. Therefore, these microorganisms have a potential use in diverse biotechnological and bioremediation cellulose degradation processes. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-07-20 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/13555 10.34117/bjdv6n7-506 |
url |
https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/13555 |
identifier_str_mv |
10.34117/bjdv6n7-506 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/13555/11358 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Brazilian Journal of Development info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Brazilian Journal of Development |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Brazilian Journals Publicações de Periódicos e Editora Ltda. |
publisher.none.fl_str_mv |
Brazilian Journals Publicações de Periódicos e Editora Ltda. |
dc.source.none.fl_str_mv |
Brazilian Journal of Development; Vol. 6 No. 7 (2020); 48831-48851 Brazilian Journal of Development; Vol. 6 Núm. 7 (2020); 48831-48851 Brazilian Journal of Development; v. 6 n. 7 (2020); 48831-48851 2525-8761 reponame:Revista Veras instname:Instituto Superior de Educação Vera Cruz (VeraCruz) instacron:VERACRUZ |
instname_str |
Instituto Superior de Educação Vera Cruz (VeraCruz) |
instacron_str |
VERACRUZ |
institution |
VERACRUZ |
reponame_str |
Revista Veras |
collection |
Revista Veras |
repository.name.fl_str_mv |
Revista Veras - Instituto Superior de Educação Vera Cruz (VeraCruz) |
repository.mail.fl_str_mv |
||revistaveras@veracruz.edu.br |
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1813645465877479424 |