Bioprospection of cellulose-decomposers soil bacteria / Bioprospecção de bactérias do solo degradadoras de celulose

Detalhes bibliográficos
Autor(a) principal: Hernández, Yohana Melania López
Data de Publicação: 2020
Outros Autores: Kuss, Anelise Vicentini, Peil, Greice Hartwig Schwanke, Villarreal, José Pablo, Gamaro, Giovana Duzzo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista Veras
Texto Completo: https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/13555
Resumo: Soil microorganisms have a high bacterial biodiversity and an important group is the cellulose-decomposers bacteria, which through their endoglucanases, exoglucanases and ?-glycosidases enzymatic complexes are responsible for the planet organic biomass degradation. Therefore, to understand and apply their potentials in the biotechnology area, is necessary to know their biologic, biochemical and environmental characteristics and proprieties of the microorganisms involved in these degradation processes. Ten bacteria isolates where obtained. To verify the degradation, the filter paper and solid medium Luria-Bertani was used. In the extracellular enzymatic production analysis, the substrate used was the Whatman N°1 filter paper for the ß-1,4 exoglucanase and carboxymethyl cellulose for the ß-1,4 endoglucanase. Also the microbial biomass analysis was made. The results showed that, the most effective cellulose degradation isolates having as substrates, the BC5 and the BC2 bacteria respectively. In the endoglucanase ß-1,4 enzymatic activity was the BC5 and for the ß-1,4 exoglucanase was the BC8, meanwhile for the microbial biomass production, the best result was for the BC9 isolate bacteria. The results showed that between the different bacteria species from the different locals, significant differences were presented, when each of the analysis were compared and therefore this can be associated to the different types of ecosystems and to the biochemical and environmental characteristics in each type of soil, having different mechanisms directly related with the cellulose degradation. Therefore, these microorganisms have a potential use in diverse biotechnological and bioremediation cellulose degradation processes. 
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spelling Bioprospection of cellulose-decomposers soil bacteria / Bioprospecção de bactérias do solo degradadoras de celulosecellulolytic enzymescellulolytic microorganismsendoglucanaseexoglucanasesoilmicrobiology environmental.Soil microorganisms have a high bacterial biodiversity and an important group is the cellulose-decomposers bacteria, which through their endoglucanases, exoglucanases and ?-glycosidases enzymatic complexes are responsible for the planet organic biomass degradation. Therefore, to understand and apply their potentials in the biotechnology area, is necessary to know their biologic, biochemical and environmental characteristics and proprieties of the microorganisms involved in these degradation processes. Ten bacteria isolates where obtained. To verify the degradation, the filter paper and solid medium Luria-Bertani was used. In the extracellular enzymatic production analysis, the substrate used was the Whatman N°1 filter paper for the ß-1,4 exoglucanase and carboxymethyl cellulose for the ß-1,4 endoglucanase. Also the microbial biomass analysis was made. The results showed that, the most effective cellulose degradation isolates having as substrates, the BC5 and the BC2 bacteria respectively. In the endoglucanase ß-1,4 enzymatic activity was the BC5 and for the ß-1,4 exoglucanase was the BC8, meanwhile for the microbial biomass production, the best result was for the BC9 isolate bacteria. The results showed that between the different bacteria species from the different locals, significant differences were presented, when each of the analysis were compared and therefore this can be associated to the different types of ecosystems and to the biochemical and environmental characteristics in each type of soil, having different mechanisms directly related with the cellulose degradation. Therefore, these microorganisms have a potential use in diverse biotechnological and bioremediation cellulose degradation processes. Brazilian Journals Publicações de Periódicos e Editora Ltda.2020-07-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/1355510.34117/bjdv6n7-506Brazilian Journal of Development; Vol. 6 No. 7 (2020); 48831-48851Brazilian Journal of Development; Vol. 6 Núm. 7 (2020); 48831-48851Brazilian Journal of Development; v. 6 n. 7 (2020); 48831-488512525-8761reponame:Revista Verasinstname:Instituto Superior de Educação Vera Cruz (VeraCruz)instacron:VERACRUZenghttps://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/13555/11358Copyright (c) 2020 Brazilian Journal of Developmentinfo:eu-repo/semantics/openAccessHernández, Yohana Melania LópezKuss, Anelise VicentiniPeil, Greice Hartwig SchwankeVillarreal, José PabloGamaro, Giovana Duzzo2020-08-11T15:17:25Zoai:ojs2.ojs.brazilianjournals.com.br:article/13555Revistahttp://site.veracruz.edu.br:8087/instituto/revistaveras/index.php/revistaveras/PRIhttp://site.veracruz.edu.br:8087/instituto/revistaveras/index.php/revistaveras/oai||revistaveras@veracruz.edu.br2236-57292236-5729opendoar:2024-10-15T16:08:10.519738Revista Veras - Instituto Superior de Educação Vera Cruz (VeraCruz)false
dc.title.none.fl_str_mv Bioprospection of cellulose-decomposers soil bacteria / Bioprospecção de bactérias do solo degradadoras de celulose
title Bioprospection of cellulose-decomposers soil bacteria / Bioprospecção de bactérias do solo degradadoras de celulose
spellingShingle Bioprospection of cellulose-decomposers soil bacteria / Bioprospecção de bactérias do solo degradadoras de celulose
Hernández, Yohana Melania López
cellulolytic enzymes
cellulolytic microorganisms
endoglucanase
exoglucanase
soil
microbiology environmental.
title_short Bioprospection of cellulose-decomposers soil bacteria / Bioprospecção de bactérias do solo degradadoras de celulose
title_full Bioprospection of cellulose-decomposers soil bacteria / Bioprospecção de bactérias do solo degradadoras de celulose
title_fullStr Bioprospection of cellulose-decomposers soil bacteria / Bioprospecção de bactérias do solo degradadoras de celulose
title_full_unstemmed Bioprospection of cellulose-decomposers soil bacteria / Bioprospecção de bactérias do solo degradadoras de celulose
title_sort Bioprospection of cellulose-decomposers soil bacteria / Bioprospecção de bactérias do solo degradadoras de celulose
author Hernández, Yohana Melania López
author_facet Hernández, Yohana Melania López
Kuss, Anelise Vicentini
Peil, Greice Hartwig Schwanke
Villarreal, José Pablo
Gamaro, Giovana Duzzo
author_role author
author2 Kuss, Anelise Vicentini
Peil, Greice Hartwig Schwanke
Villarreal, José Pablo
Gamaro, Giovana Duzzo
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Hernández, Yohana Melania López
Kuss, Anelise Vicentini
Peil, Greice Hartwig Schwanke
Villarreal, José Pablo
Gamaro, Giovana Duzzo
dc.subject.por.fl_str_mv cellulolytic enzymes
cellulolytic microorganisms
endoglucanase
exoglucanase
soil
microbiology environmental.
topic cellulolytic enzymes
cellulolytic microorganisms
endoglucanase
exoglucanase
soil
microbiology environmental.
description Soil microorganisms have a high bacterial biodiversity and an important group is the cellulose-decomposers bacteria, which through their endoglucanases, exoglucanases and ?-glycosidases enzymatic complexes are responsible for the planet organic biomass degradation. Therefore, to understand and apply their potentials in the biotechnology area, is necessary to know their biologic, biochemical and environmental characteristics and proprieties of the microorganisms involved in these degradation processes. Ten bacteria isolates where obtained. To verify the degradation, the filter paper and solid medium Luria-Bertani was used. In the extracellular enzymatic production analysis, the substrate used was the Whatman N°1 filter paper for the ß-1,4 exoglucanase and carboxymethyl cellulose for the ß-1,4 endoglucanase. Also the microbial biomass analysis was made. The results showed that, the most effective cellulose degradation isolates having as substrates, the BC5 and the BC2 bacteria respectively. In the endoglucanase ß-1,4 enzymatic activity was the BC5 and for the ß-1,4 exoglucanase was the BC8, meanwhile for the microbial biomass production, the best result was for the BC9 isolate bacteria. The results showed that between the different bacteria species from the different locals, significant differences were presented, when each of the analysis were compared and therefore this can be associated to the different types of ecosystems and to the biochemical and environmental characteristics in each type of soil, having different mechanisms directly related with the cellulose degradation. Therefore, these microorganisms have a potential use in diverse biotechnological and bioremediation cellulose degradation processes. 
publishDate 2020
dc.date.none.fl_str_mv 2020-07-20
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/13555
10.34117/bjdv6n7-506
url https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/13555
identifier_str_mv 10.34117/bjdv6n7-506
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/13555/11358
dc.rights.driver.fl_str_mv Copyright (c) 2020 Brazilian Journal of Development
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Brazilian Journal of Development
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Brazilian Journals Publicações de Periódicos e Editora Ltda.
publisher.none.fl_str_mv Brazilian Journals Publicações de Periódicos e Editora Ltda.
dc.source.none.fl_str_mv Brazilian Journal of Development; Vol. 6 No. 7 (2020); 48831-48851
Brazilian Journal of Development; Vol. 6 Núm. 7 (2020); 48831-48851
Brazilian Journal of Development; v. 6 n. 7 (2020); 48831-48851
2525-8761
reponame:Revista Veras
instname:Instituto Superior de Educação Vera Cruz (VeraCruz)
instacron:VERACRUZ
instname_str Instituto Superior de Educação Vera Cruz (VeraCruz)
instacron_str VERACRUZ
institution VERACRUZ
reponame_str Revista Veras
collection Revista Veras
repository.name.fl_str_mv Revista Veras - Instituto Superior de Educação Vera Cruz (VeraCruz)
repository.mail.fl_str_mv ||revistaveras@veracruz.edu.br
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