Performance of chimeric trypanosoma cruzi antigens in serological screening for chagas disease in blood banks
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/52081 |
Resumo: | Coordenação de Aperfeiçoamento de Pessoal de Nível-Brasil (CAPES). Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). Inova Fiocruz. Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). |
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Santos, Emily Ferreira dosSilva, Ângelo Antônio OliveiraFreitas, Natália Erdens MaronLeony, Leonardo MaiaDaltro, Ramona TavaresSantos, Carlos Antônio de Souza TelesAlmeida, Maria da Conceição Chagas deAraújo, Fernando Luiz Vieira deCeledon, Paola Alejandra FioraniKrieger, Marco AurélioZanchin, Nilson Ivo ToninReis, Mitermayer Galvão dosSantos, Fred Luciano Neves2022-04-07T13:26:58Z2022-04-07T13:26:58Z2022SANTOS, Emily Ferreira dos et al. Performance of chimeric trypanosoma cruzi antigens in serological screening for chagas disease in blood banks. Frontiers in Medicine, v. 9, p. 1-14, 2022.2296-858Xhttps://www.arca.fiocruz.br/handle/icict/5208110.3389/fmed.2022.852864Coordenação de Aperfeiçoamento de Pessoal de Nível-Brasil (CAPES). Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). Inova Fiocruz. Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Público Avançado de Saúde. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Público Avançado de Saúde. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Público Avançado de Saúde. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Público Avançado de Saúde. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Público Avançado de Saúde. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Centro de Integração de Dados e Conhecimento em Saúde. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Epidemiologia Molecular e Bioestatística. Salvador, BA, Brasil.Fundação de Hematologia e Hemoterapia do Estado da Bahia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Molecular de Tripanossomatídeos. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciência e Tecnologia Aplicada em Saúde. Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Programa Translacional Integrado em Doença de Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Biologia Estrutural e Engenharia de Proteínas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Programa Translacional Integrado em Doença de Chagas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Yale University. Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, United States.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Público Avançado de Saúde. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Programa Translacional Integrado em Doença de Chagas. Rio de Janeiro, RJ, Brasil.Chagas disease (CD) is among the top 10 causes of inability to blood donation. Blood donation centers screen for anti-Trypanosoma cruzi antibodies using highly sensitive immunoenzymatic (ELISA) or chemiluminescent methods, which can lead to false positive results. Since positive samples cannot be used, to avoid the loss of valuable blood donations, it is necessary to improve specificity without reducing the sensitivity of the tests used for blood screening. For this purpose, our group has developed four chimeric proteins (IBMP-8.1, IBMP-8.2, IBMP-8.3, and IBMP-8.4) that have been evaluated in phase I and II studies with high performance and low cross-reactivity rates. The study included a panel of 5,014 serum samples collected from volunteer blood donors at the Hematology and Hemotherapy Foundation of the State of Bahia (Brazil). They were subjected to the detection of anti-T. cruzi antibodies, using all four IBMP antigens individually and latent class analysis (LCA) as a reference test, since there is no gold standard test for this purpose. Considering the sample size analyzed, LCA classified 4,993 (99.6%) samples as T. cruzi-negative and 21 (0.42%) as T. cruzi-positive. Sensitivity values ranged from 85.71% for IBMP-8.1 and 90.48% for IBMP-8.2–95.24% for IBMP-8.3 and 100% for IBMP-8.4, while specificity ranged from 99.98% for IBMP-8.3 and IBMP-8.4–100% for IBMP-8.1 and IBMP-8.2. Accuracy values ranged from 99.4 to 99.98%. The pretest probability for the molecules was 0.42, whereas the positive posttest probability ranged from 95.24 to 99.95% and the negative posttest probability ranged from 0.00001 to 0.0006% for all antigens. The higher odds ratio diagnosis was found for IBMP-8.4, which has been shown to be a safe single antigen for serological screening of CD in blood samples. The use of chimeric IBMP antigens is an alternative to reduce the number of bags discarded due to false-positive results. These molecules have high diagnostic performance and were shown to be suitable for use in screening CD in blood banks, isolated (IBMP-8.4) or in combination; and their use in blood banks could significantly reduce unnecessary disposal of blood bags or the risk of T. cruzi transmission.engFrontiers MediaDoença de chagasBancos de sangueSorologiaDiagnósticoChagas diseaseBlood banksSerologyDiagnosisEnfermedad de chagasBancos de sangreSerologíaDiagnósticoMaladie de chagasBanques de sangSérologieDiagnosticPerformance of chimeric trypanosoma cruzi antigens in serological screening for chagas disease in blood banksinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/52081/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSantos, Emily F. dos - Performance of Chimeric Trypanosoma cruzi.pdfSantos, Emily F. dos - Performance of Chimeric Trypanosoma cruzi.pdfapplication/pdf6861934https://www.arca.fiocruz.br/bitstream/icict/52081/2/Santos%2c%20%20Emily%20F.%20dos%20-%20Performance%20of%20Chimeric%20Trypanosoma%20cruzi.pdf10b836f23f091f550d2a937271486513MD52icict/520812023-03-15 14:34:34.206oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:34:34Repositório Institucional da FIOCRUZ (ARCA) - 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| dc.title.pt_BR.fl_str_mv |
Performance of chimeric trypanosoma cruzi antigens in serological screening for chagas disease in blood banks |
| title |
Performance of chimeric trypanosoma cruzi antigens in serological screening for chagas disease in blood banks |
| spellingShingle |
Performance of chimeric trypanosoma cruzi antigens in serological screening for chagas disease in blood banks Santos, Emily Ferreira dos Doença de chagas Bancos de sangue Sorologia Diagnóstico Chagas disease Blood banks Serology Diagnosis Enfermedad de chagas Bancos de sangre Serología Diagnóstico Maladie de chagas Banques de sang Sérologie Diagnostic |
| title_short |
Performance of chimeric trypanosoma cruzi antigens in serological screening for chagas disease in blood banks |
| title_full |
Performance of chimeric trypanosoma cruzi antigens in serological screening for chagas disease in blood banks |
| title_fullStr |
Performance of chimeric trypanosoma cruzi antigens in serological screening for chagas disease in blood banks |
| title_full_unstemmed |
Performance of chimeric trypanosoma cruzi antigens in serological screening for chagas disease in blood banks |
| title_sort |
Performance of chimeric trypanosoma cruzi antigens in serological screening for chagas disease in blood banks |
| author |
Santos, Emily Ferreira dos |
| author_facet |
Santos, Emily Ferreira dos Silva, Ângelo Antônio Oliveira Freitas, Natália Erdens Maron Leony, Leonardo Maia Daltro, Ramona Tavares Santos, Carlos Antônio de Souza Teles Almeida, Maria da Conceição Chagas de Araújo, Fernando Luiz Vieira de Celedon, Paola Alejandra Fiorani Krieger, Marco Aurélio Zanchin, Nilson Ivo Tonin Reis, Mitermayer Galvão dos Santos, Fred Luciano Neves |
| author_role |
author |
| author2 |
Silva, Ângelo Antônio Oliveira Freitas, Natália Erdens Maron Leony, Leonardo Maia Daltro, Ramona Tavares Santos, Carlos Antônio de Souza Teles Almeida, Maria da Conceição Chagas de Araújo, Fernando Luiz Vieira de Celedon, Paola Alejandra Fiorani Krieger, Marco Aurélio Zanchin, Nilson Ivo Tonin Reis, Mitermayer Galvão dos Santos, Fred Luciano Neves |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Emily Ferreira dos Silva, Ângelo Antônio Oliveira Freitas, Natália Erdens Maron Leony, Leonardo Maia Daltro, Ramona Tavares Santos, Carlos Antônio de Souza Teles Almeida, Maria da Conceição Chagas de Araújo, Fernando Luiz Vieira de Celedon, Paola Alejandra Fiorani Krieger, Marco Aurélio Zanchin, Nilson Ivo Tonin Reis, Mitermayer Galvão dos Santos, Fred Luciano Neves |
| dc.subject.other.pt_BR.fl_str_mv |
Doença de chagas Bancos de sangue Sorologia Diagnóstico |
| topic |
Doença de chagas Bancos de sangue Sorologia Diagnóstico Chagas disease Blood banks Serology Diagnosis Enfermedad de chagas Bancos de sangre Serología Diagnóstico Maladie de chagas Banques de sang Sérologie Diagnostic |
| dc.subject.en.pt_BR.fl_str_mv |
Chagas disease Blood banks Serology Diagnosis |
| dc.subject.es.pt_BR.fl_str_mv |
Enfermedad de chagas Bancos de sangre Serología Diagnóstico |
| dc.subject.fr.pt_BR.fl_str_mv |
Maladie de chagas Banques de sang Sérologie Diagnostic |
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Coordenação de Aperfeiçoamento de Pessoal de Nível-Brasil (CAPES). Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). Inova Fiocruz. Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). |
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2022 |
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2022-04-07T13:26:58Z |
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2022-04-07T13:26:58Z |
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2022 |
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info:eu-repo/semantics/article |
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SANTOS, Emily Ferreira dos et al. Performance of chimeric trypanosoma cruzi antigens in serological screening for chagas disease in blood banks. Frontiers in Medicine, v. 9, p. 1-14, 2022. |
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https://www.arca.fiocruz.br/handle/icict/52081 |
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2296-858X |
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10.3389/fmed.2022.852864 |
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SANTOS, Emily Ferreira dos et al. Performance of chimeric trypanosoma cruzi antigens in serological screening for chagas disease in blood banks. Frontiers in Medicine, v. 9, p. 1-14, 2022. 2296-858X 10.3389/fmed.2022.852864 |
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