Assessment of Liaison XL Murex Chagas diagnostic performance in blood screening for Chagas disease using a reference array of chimeric antigens
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/48579 |
Resumo: | Conselho Nacional de Desenvolvimento Científico e Tecnologico, Grant/Award Numbers: 309263/2020-4, 312195/2015-0, 590032/2011-9 |
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Santos, Emily Ferreira dosLeony, Leonardo MaiaSilva, Ângelo Antônio OliveiraDaltro, Ramona TavaresFreitas, Natália Erdens Maron deVasconcelos, Larissa de Carvalho MedradoAraújo, Fernando Luiz Vieira deCeledon, Paola Alejandra FioraniKrieger, Marco AurélioZanchin, Nilson Ivo ToninSantos, Fred Luciano Neves2021-08-11T17:08:32Z2021-08-11T17:08:32Z2021SANTOS, Emily Ferreira dos et al. Assessment of Liaison XL Murex Chagas diagnostic performance in blood screening for Chagas disease using a reference array of chimeric antigens. Transfusion, v. 61, n. 9, p. 2701-2709, Sept. 2021.0041-1132https://www.arca.fiocruz.br/handle/icict/4857910.1111/trf.165831537-2995Conselho Nacional de Desenvolvimento Científico e Tecnologico, Grant/Award Numbers: 309263/2020-4, 312195/2015-0, 590032/2011-9Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Grant/Award Number: 001Fundação de Amparo à Pesquisa do Estado da Bahia, Grant/Award Numbers: BOL0543/2020, BOL0932/2020Inova Fiocruz/VPPCB, Grant/Award Number: VPPCB-008-FIO-18-2-20Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil.Fundação de Hematologia e Hemoterapia do Estado da Bahia. Salvador, BA, Brasil.Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil.Background: Chagas disease (CD) serological screening at blood banks is usually performed by a single highly sensitive serological assay, with chemiluminescent immunoassays (CLIAs) being the method of choice. CLIAs employ recombinant, fusion peptides and/or chimeric antigens that selectively capture anti-Trypanosoma cruzi antibodies. However, despite high sensitivity, the ability of these tests to identify CD-positive cases should be evaluated against T. cruzi strains circulating in specific locales. Herein, we used a latent class analysis (LCA) approach employing an array of four chimeric antigens to assess the diagnostic performance of the Liaison XL Murex Chagas CLIA for the detection of anti-T. cruzi IgG in serum samples. Study Design and Methods: The study included a panel of 5014 serum samples collected from volunteer blood donors at the Hematology and Hemotherapy Foundation of the State of Bahia, submitted to anti-T. cruzi antibody detection using Liaison Chagas CLIA and LCA as a reference test in the absence of a gold standard. Results: LCA classified 4993 samples as negative, while positivity for T. cruzi antibodies was predicted in 21 samples. Compared with LCA, CLIA demonstrated sensitivity and specificity of 76.2% and 99.5%, respectively, providing an overall accuracy of 99.4%. Discussion: In blood banks lacking a de facto highly sensitive screening immunoassay, the low sensitivity offered by Liaison Chagas CLIA renders it unsuitable for standalone use in serological screening procedures for CD. Moreover, blood banks are encouraged to carefully assess the ability of diagnostic methods to identify local T. cruzi strains in circulation.engWileyDoença de ChagasLuminescênciaImunoensaioAnálise de classes latentesTriagem sorológicaChagas diseaseChemiluminescenceImmunoassayLatent class analysisSerological screeningEnfermedad de ChagasLuminiscenciaInmunoensayoAnálisis de Clases LatentesSerologíaMaladie de ChagasLuminescenceDosage immunologiqueAnalyse de structure latenteSérologieDoença de ChagasLuminescênciaImunoensaioAnálise de Classes LatentesSorologiaAssessment of Liaison XL Murex Chagas diagnostic performance in blood screening for Chagas disease using a reference array of chimeric antigensinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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| dc.title.pt_BR.fl_str_mv |
Assessment of Liaison XL Murex Chagas diagnostic performance in blood screening for Chagas disease using a reference array of chimeric antigens |
| title |
Assessment of Liaison XL Murex Chagas diagnostic performance in blood screening for Chagas disease using a reference array of chimeric antigens |
| spellingShingle |
Assessment of Liaison XL Murex Chagas diagnostic performance in blood screening for Chagas disease using a reference array of chimeric antigens Santos, Emily Ferreira dos Doença de Chagas Luminescência Imunoensaio Análise de classes latentes Triagem sorológica Chagas disease Chemiluminescence Immunoassay Latent class analysis Serological screening Enfermedad de Chagas Luminiscencia Inmunoensayo Análisis de Clases Latentes Serología Maladie de Chagas Luminescence Dosage immunologique Analyse de structure latente Sérologie Doença de Chagas Luminescência Imunoensaio Análise de Classes Latentes Sorologia |
| title_short |
Assessment of Liaison XL Murex Chagas diagnostic performance in blood screening for Chagas disease using a reference array of chimeric antigens |
| title_full |
Assessment of Liaison XL Murex Chagas diagnostic performance in blood screening for Chagas disease using a reference array of chimeric antigens |
| title_fullStr |
Assessment of Liaison XL Murex Chagas diagnostic performance in blood screening for Chagas disease using a reference array of chimeric antigens |
| title_full_unstemmed |
Assessment of Liaison XL Murex Chagas diagnostic performance in blood screening for Chagas disease using a reference array of chimeric antigens |
| title_sort |
Assessment of Liaison XL Murex Chagas diagnostic performance in blood screening for Chagas disease using a reference array of chimeric antigens |
| author |
Santos, Emily Ferreira dos |
| author_facet |
Santos, Emily Ferreira dos Leony, Leonardo Maia Silva, Ângelo Antônio Oliveira Daltro, Ramona Tavares Freitas, Natália Erdens Maron de Vasconcelos, Larissa de Carvalho Medrado Araújo, Fernando Luiz Vieira de Celedon, Paola Alejandra Fiorani Krieger, Marco Aurélio Zanchin, Nilson Ivo Tonin Santos, Fred Luciano Neves |
| author_role |
author |
| author2 |
Leony, Leonardo Maia Silva, Ângelo Antônio Oliveira Daltro, Ramona Tavares Freitas, Natália Erdens Maron de Vasconcelos, Larissa de Carvalho Medrado Araújo, Fernando Luiz Vieira de Celedon, Paola Alejandra Fiorani Krieger, Marco Aurélio Zanchin, Nilson Ivo Tonin Santos, Fred Luciano Neves |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Emily Ferreira dos Leony, Leonardo Maia Silva, Ângelo Antônio Oliveira Daltro, Ramona Tavares Freitas, Natália Erdens Maron de Vasconcelos, Larissa de Carvalho Medrado Araújo, Fernando Luiz Vieira de Celedon, Paola Alejandra Fiorani Krieger, Marco Aurélio Zanchin, Nilson Ivo Tonin Santos, Fred Luciano Neves |
| dc.subject.other.pt_BR.fl_str_mv |
Doença de Chagas Luminescência Imunoensaio Análise de classes latentes Triagem sorológica |
| topic |
Doença de Chagas Luminescência Imunoensaio Análise de classes latentes Triagem sorológica Chagas disease Chemiluminescence Immunoassay Latent class analysis Serological screening Enfermedad de Chagas Luminiscencia Inmunoensayo Análisis de Clases Latentes Serología Maladie de Chagas Luminescence Dosage immunologique Analyse de structure latente Sérologie Doença de Chagas Luminescência Imunoensaio Análise de Classes Latentes Sorologia |
| dc.subject.en.en_US.fl_str_mv |
Chagas disease Chemiluminescence Immunoassay Latent class analysis Serological screening |
| dc.subject.es.pt_BR.fl_str_mv |
Enfermedad de Chagas Luminiscencia Inmunoensayo Análisis de Clases Latentes Serología |
| dc.subject.fr.pt_BR.fl_str_mv |
Maladie de Chagas Luminescence Dosage immunologique Analyse de structure latente Sérologie |
| dc.subject.decs.pt_BR.fl_str_mv |
Doença de Chagas Luminescência Imunoensaio Análise de Classes Latentes Sorologia |
| description |
Conselho Nacional de Desenvolvimento Científico e Tecnologico, Grant/Award Numbers: 309263/2020-4, 312195/2015-0, 590032/2011-9 |
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2021 |
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2021-08-11T17:08:32Z |
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2021-08-11T17:08:32Z |
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2021 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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SANTOS, Emily Ferreira dos et al. Assessment of Liaison XL Murex Chagas diagnostic performance in blood screening for Chagas disease using a reference array of chimeric antigens. Transfusion, v. 61, n. 9, p. 2701-2709, Sept. 2021. |
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https://www.arca.fiocruz.br/handle/icict/48579 |
| dc.identifier.issn.pt_BR.fl_str_mv |
0041-1132 |
| dc.identifier.doi.pt_BR.fl_str_mv |
10.1111/trf.16583 |
| dc.identifier.eissn.pt_BR.fl_str_mv |
1537-2995 |
| identifier_str_mv |
SANTOS, Emily Ferreira dos et al. Assessment of Liaison XL Murex Chagas diagnostic performance in blood screening for Chagas disease using a reference array of chimeric antigens. Transfusion, v. 61, n. 9, p. 2701-2709, Sept. 2021. 0041-1132 10.1111/trf.16583 1537-2995 |
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https://www.arca.fiocruz.br/handle/icict/48579 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Wiley |
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Wiley |
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