Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection

Bibliographic Details
Main Author: Arruda, Mauro Maciel de
Publication Date: 2016
Other Authors: Figueiredo, Fabiano Borges, Marcelino, Andreza Pain, Barbosa, José Ronaldo, Werneck, Guilherme Loureiro, Noronha, Elza Ferreira, Romero, Gustavo Adolfo Sierra
Format: Article
Language: eng
Source: Repositório Institucional da FIOCRUZ (ARCA)
Download full: https://www.arca.fiocruz.br/handle/icict/32590
Summary: Universidade de Brasília. Núcleo de Medicina Tropical. Brasília, DF, Brasil.
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spelling Arruda, Mauro Maciel deFigueiredo, Fabiano BorgesMarcelino, Andreza PainBarbosa, José RonaldoWerneck, Guilherme LoureiroNoronha, Elza FerreiraRomero, Gustavo Adolfo Sierra2019-04-18T13:54:50Z2019-04-18T13:54:50Z2016ARRUDA, Mauro Maciel de et al. Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 111, n. 3, p. 168-173, Mar. 2016.0074-0276https://www.arca.fiocruz.br/handle/icict/3259010.1590/0074-027601503641678-8060engInstituto Oswaldo CruzSensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de Brasília. Núcleo de Medicina Tropical. Brasília, DF, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatozoonoses em Animais Domésticos, Rio de Janeiro, RJ, Brasil.Fundação Ezequiel Dias. Belo Horizonte, MG, Brasil.Fundação Ezequiel Dias. Belo Horizonte, MG, Brasil.Universidade do Estado do Rio de Janeiro. Instituto de Medicina Social. Rio de Janeiro, RJ, Brasil.Universidade de Brasília. Núcleo de Medicina Tropical. Brasília, DF, Brasil.Universidade de Brasília. Núcleo de Medicina Tropical. Brasília, DF, Brasil.In Brazil, human and canine visceral leishmaniasis (CVL) caused by Leishmania infantum has undergone urbanisation since 1980, constituting a public health problem, and serological tests are tools of choice for identifying infected dogs. Until recently, the Brazilian zoonoses control program recommended enzyme-linked immunosorbent assays (ELISA) and indirect immunofluorescence assays (IFA) as the screening and confirmatory methods, respectively, for the detection of canine infection. The purpose of this study was to estimate the accuracy of ELISA and IFA in parallel or serial combinations. The reference standard comprised the results of direct visualisation of parasites in histological sections, immunohistochemical test, or isolation of the parasite in culture. Samples from 98 cases and 1,327 noncases were included. Individually, both tests presented sensitivity of 91.8% and 90.8%, and specificity of 83.4 and 53.4%, for the ELISA and IFA, respectively. When tests were used in parallel combination, sensitivity attained 99.2%, while specificity dropped to 44.8%. When used in serial combination (ELISA followed by IFA), decreased sensitivity (83.3%) and increased specificity (92.5%) were observed. Serial testing approach improved specificity with moderate loss in sensitivity. This strategy could partially fulfill the needs of public health and dog owners for a more accurate diagnosis of CVL.DogsSensitivitySpecificityVisceral leishmaniasisLeishmania infantumSerologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83104https://www.arca.fiocruz.br/bitstream/icict/32590/1/license.txt79178e5f2a0eb066867a274556814938MD51ORIGINALve_Arruda_Mauro_etal_INI_2016.pdfve_Arruda_Mauro_etal_INI_2016.pdfapplication/pdf364751https://www.arca.fiocruz.br/bitstream/icict/32590/2/ve_Arruda_Mauro_etal_INI_2016.pdf360224b1c9e1b6d55ebebba405c8f2a8MD52TEXTve_Arruda_Mauro_etal_INI_2016.pdf.txtve_Arruda_Mauro_etal_INI_2016.pdf.txtExtracted texttext/plain29805https://www.arca.fiocruz.br/bitstream/icict/32590/3/ve_Arruda_Mauro_etal_INI_2016.pdf.txt56241cd238d2656619c5da14b5ebef5bMD53icict/325902019-04-19 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dc.title.pt_BR.fl_str_mv Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection
title Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection
spellingShingle Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection
Arruda, Mauro Maciel de
Dogs
Sensitivity
Specificity
Visceral leishmaniasis
Leishmania infantum
Serology
title_short Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection
title_full Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection
title_fullStr Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection
title_full_unstemmed Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection
title_sort Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection
author Arruda, Mauro Maciel de
author_facet Arruda, Mauro Maciel de
Figueiredo, Fabiano Borges
Marcelino, Andreza Pain
Barbosa, José Ronaldo
Werneck, Guilherme Loureiro
Noronha, Elza Ferreira
Romero, Gustavo Adolfo Sierra
author_role author
author2 Figueiredo, Fabiano Borges
Marcelino, Andreza Pain
Barbosa, José Ronaldo
Werneck, Guilherme Loureiro
Noronha, Elza Ferreira
Romero, Gustavo Adolfo Sierra
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Arruda, Mauro Maciel de
Figueiredo, Fabiano Borges
Marcelino, Andreza Pain
Barbosa, José Ronaldo
Werneck, Guilherme Loureiro
Noronha, Elza Ferreira
Romero, Gustavo Adolfo Sierra
dc.subject.en.pt_BR.fl_str_mv Dogs
Sensitivity
Specificity
Visceral leishmaniasis
Leishmania infantum
Serology
topic Dogs
Sensitivity
Specificity
Visceral leishmaniasis
Leishmania infantum
Serology
description Universidade de Brasília. Núcleo de Medicina Tropical. Brasília, DF, Brasil.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2019-04-18T13:54:50Z
dc.date.available.fl_str_mv 2019-04-18T13:54:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ARRUDA, Mauro Maciel de et al. Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 111, n. 3, p. 168-173, Mar. 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32590
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760150364
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv ARRUDA, Mauro Maciel de et al. Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 111, n. 3, p. 168-173, Mar. 2016.
0074-0276
10.1590/0074-02760150364
1678-8060
url https://www.arca.fiocruz.br/handle/icict/32590
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz
publisher.none.fl_str_mv Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/32590/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/32590/2/ve_Arruda_Mauro_etal_INI_2016.pdf
https://www.arca.fiocruz.br/bitstream/icict/32590/3/ve_Arruda_Mauro_etal_INI_2016.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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