Crystal structure of Staphylococcus aureus exfoliative toxin D-like protein: Structural basis for the high specificity of exfoliative toxins

Detalhes bibliográficos
Autor(a) principal: Mariutti, Ricardo B.
Data de Publicação: 2015
Outros Autores: Souza, Tatiana de Arruda Campos Brasil de, Ullah, Anwar, Caruso, Icaro P., Moraes, Fábio R. de, Zanphorlin, Leticia Maria, Tartaglia, Natayme R., Seyffert, Nubia, Azevedo, Vasco A., Loir, Yves Le, Murakami, Mario Tyago, Arni, Raghuvir K.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/16235
Resumo: Universidade Estadual Paulista. Instituto de Biociências, Letras e Ciências Exatas. Departamento de Física. Centro Multiusuário para Inovação Biomolecular. São José do Rio Preto, SP, Brasil.
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spelling Mariutti, Ricardo B.Souza, Tatiana de Arruda Campos Brasil deUllah, AnwarCaruso, Icaro P.Moraes, Fábio R. deZanphorlin, Leticia MariaTartaglia, Natayme R.Seyffert, NubiaAzevedo, Vasco A.Loir, Yves LeMurakami, Mario TyagoArni, Raghuvir K.2016-10-13T19:07:57Z2016-10-13T19:07:57Z2015MARIUTTI, Ricardo B. et al. Crystal structure of Staphylococcus aureus exfoliative toxin D-like protein: Structural basis for the high specificity of exfoliative toxins. Biochemical and Biophysical Research Communications, v. 467, n. 1, p. 171-177, Nov. 2015.0006-291Xhttps://www.arca.fiocruz.br/handle/icict/1623510.1016/j.bbrc.2015.08.083engW. BaumeisterCrystal structure of Staphylococcus aureus exfoliative toxin D-like protein: Structural basis for the high specificity of exfoliative toxinsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual Paulista. Instituto de Biociências, Letras e Ciências Exatas. Departamento de Física. Centro Multiusuário para Inovação Biomolecular. São José do Rio Preto, SP, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Estadual Paulista. Instituto de Biociências, Letras e Ciências Exatas. Departamento de Física. Centro Multiusuário para Inovação Biomolecular. São José do Rio Preto, SP, Brasil.Universidade Estadual Paulista. Instituto de Biociências, Letras e Ciências Exatas. Departamento de Física. Centro Multiusuário para Inovação Biomolecular. São José do Rio Preto, SP, Brasil.Universidade Estadual Paulista. Instituto de Biociências, Letras e Ciências Exatas. Departamento de Física. Centro Multiusuário para Inovação Biomolecular. São José do Rio Preto, SP, Brasil.Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Laboratório de Ciência e Tecnologia de Bioetanol. Campinas, SP, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Genética Celular e Molecular. Belo Horizonte, MG, Brasil / French National Institute for Agricultural Research. Laboratoire du Science et Technologie du Lait et de l’œuf. Rennes, France / Agrocampus Ouest. Rennes, France.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Genética Celular e Molecular. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Genética Celular e Molecular. Belo Horizonte, MG, Brasil.Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Biosciências Brasileiras. Campinas, SP, Brasil.Universidade Estadual Paulista. Instituto de Biociências, Letras e Ciências Exatas. Departamento de Física. Centro Multiusuário para Inovação Biomolecular. São José do Rio Preto, SP, Brasil.Exfoliative toxins are serine proteases secreted by Staphylococcus aureus that are associated with toxin-mediated staphylococcal syndromes. To date, four different serotypes of exfoliative toxins have been identified and 3 of them (ETA, ETB, and ETD) are linked to human infection. Among these toxins, only the ETD structure remained unknown, limiting our understanding of the structural determinants for the functional differentiation between these toxins. We recently identified an ETD-like protein associated to S. aureus strains involved in mild mastitis in sheep. The crystal structure of this ETD-like protein was determined at 1.95 Å resolution and the structural analysis provide insights into the oligomerization, stability and specificity and enabled a comprehensive structural comparison with ETA and ETB. Despite the highly conserved molecular architecture, significant differences in the composition of the loops and in both the N- and C-terminal α-helices seem to define ETD-like specificity. Molecular dynamics simulations indicate that these regions defining ET specificity present different degrees of flexibility and may undergo conformational changes upon substrate recognition and binding. DLS and AUC experiments indicated that the ETD-like is monomeric in solution whereas it is present as a dimer in the asymmetric unit indicating that oligomerization is not related to functional differentiation among these toxins. Differential scanning calorimetry and circular dichroism assays demonstrated an endothermic transition centered at 52 °C, and an exothermic aggregation in temperatures up to 64 °C. All these together provide insights about the mode of action of a toxin often secreted in syndromes that are not associated with either ETA or ETB.Exfoliative toxin D-like proteinStaphylococcus aureusCrystal structureToxin-mediated staphylococcal syndromesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83074https://www.arca.fiocruz.br/bitstream/icict/16235/1/license.txt0271fb68963c017aa933c7aff60379f4MD51ORIGINALCrystal structure of Staphylococcus aureus.pdfCrystal structure of Staphylococcus aureus.pdfapplication/pdf1416047https://www.arca.fiocruz.br/bitstream/icict/16235/2/Crystal%20structure%20of%20Staphylococcus%20aureus.pdf53f125cbb3d4cf997582ffceb05b570eMD52TEXTCrystal structure of Staphylococcus aureus.pdf.txtCrystal structure of Staphylococcus aureus.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Crystal structure of Staphylococcus aureus exfoliative toxin D-like protein: Structural basis for the high specificity of exfoliative toxins
title Crystal structure of Staphylococcus aureus exfoliative toxin D-like protein: Structural basis for the high specificity of exfoliative toxins
spellingShingle Crystal structure of Staphylococcus aureus exfoliative toxin D-like protein: Structural basis for the high specificity of exfoliative toxins
Mariutti, Ricardo B.
Exfoliative toxin D-like protein
Staphylococcus aureus
Crystal structure
Toxin-mediated staphylococcal syndromes
title_short Crystal structure of Staphylococcus aureus exfoliative toxin D-like protein: Structural basis for the high specificity of exfoliative toxins
title_full Crystal structure of Staphylococcus aureus exfoliative toxin D-like protein: Structural basis for the high specificity of exfoliative toxins
title_fullStr Crystal structure of Staphylococcus aureus exfoliative toxin D-like protein: Structural basis for the high specificity of exfoliative toxins
title_full_unstemmed Crystal structure of Staphylococcus aureus exfoliative toxin D-like protein: Structural basis for the high specificity of exfoliative toxins
title_sort Crystal structure of Staphylococcus aureus exfoliative toxin D-like protein: Structural basis for the high specificity of exfoliative toxins
author Mariutti, Ricardo B.
author_facet Mariutti, Ricardo B.
Souza, Tatiana de Arruda Campos Brasil de
Ullah, Anwar
Caruso, Icaro P.
Moraes, Fábio R. de
Zanphorlin, Leticia Maria
Tartaglia, Natayme R.
Seyffert, Nubia
Azevedo, Vasco A.
Loir, Yves Le
Murakami, Mario Tyago
Arni, Raghuvir K.
author_role author
author2 Souza, Tatiana de Arruda Campos Brasil de
Ullah, Anwar
Caruso, Icaro P.
Moraes, Fábio R. de
Zanphorlin, Leticia Maria
Tartaglia, Natayme R.
Seyffert, Nubia
Azevedo, Vasco A.
Loir, Yves Le
Murakami, Mario Tyago
Arni, Raghuvir K.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mariutti, Ricardo B.
Souza, Tatiana de Arruda Campos Brasil de
Ullah, Anwar
Caruso, Icaro P.
Moraes, Fábio R. de
Zanphorlin, Leticia Maria
Tartaglia, Natayme R.
Seyffert, Nubia
Azevedo, Vasco A.
Loir, Yves Le
Murakami, Mario Tyago
Arni, Raghuvir K.
dc.subject.en.pt_BR.fl_str_mv Exfoliative toxin D-like protein
Staphylococcus aureus
Crystal structure
Toxin-mediated staphylococcal syndromes
topic Exfoliative toxin D-like protein
Staphylococcus aureus
Crystal structure
Toxin-mediated staphylococcal syndromes
description Universidade Estadual Paulista. Instituto de Biociências, Letras e Ciências Exatas. Departamento de Física. Centro Multiusuário para Inovação Biomolecular. São José do Rio Preto, SP, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-10-13T19:07:57Z
dc.date.available.fl_str_mv 2016-10-13T19:07:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARIUTTI, Ricardo B. et al. Crystal structure of Staphylococcus aureus exfoliative toxin D-like protein: Structural basis for the high specificity of exfoliative toxins. Biochemical and Biophysical Research Communications, v. 467, n. 1, p. 171-177, Nov. 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/16235
dc.identifier.issn.pt_BR.fl_str_mv 0006-291X
dc.identifier.doi.pt_BR.fl_str_mv 10.1016/j.bbrc.2015.08.083
identifier_str_mv MARIUTTI, Ricardo B. et al. Crystal structure of Staphylococcus aureus exfoliative toxin D-like protein: Structural basis for the high specificity of exfoliative toxins. Biochemical and Biophysical Research Communications, v. 467, n. 1, p. 171-177, Nov. 2015.
0006-291X
10.1016/j.bbrc.2015.08.083
url https://www.arca.fiocruz.br/handle/icict/16235
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv W. Baumeister
publisher.none.fl_str_mv W. Baumeister
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/16235/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/16235/2/Crystal%20structure%20of%20Staphylococcus%20aureus.pdf
https://www.arca.fiocruz.br/bitstream/icict/16235/3/Crystal%20structure%20of%20Staphylococcus%20aureus.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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